Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_F15
(518 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (... 147 1e-35
sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (... 145 7e-35
sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (... 144 2e-34
sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (... 138 9e-33
sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (... 136 3e-32
sp|P97343|UHMK1_MOUSE Serine/threonine-protein kinase Kist ... 62 6e-10
sp|Q8TAS1|UHMK1_HUMAN Serine/threonine-protein kinase Kist ... 62 6e-10
sp|Q63285|UHMK1_RAT Serine/threonine-protein kinase Kist (K... 62 6e-10
sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (... 55 1e-07
sp|Q8JZX4|SPF45_MOUSE Splicing factor 45 (45 kDa splicing f... 50 2e-06
>sp|Q24562|U2AF2_DROME Splicing factor U2AF 50 kDa subunit (U2 auxiliary factor 50 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 416
Score = 147 bits (372), Expect = 1e-35
Identities = 82/152 (53%), Positives = 99/152 (65%), Gaps = 4/152 (2%)
Frame = +2
Query: 11 AGLNGMQLGDKKIIVQRASIGAKHGIMPIMPFGNASFE----SLLAVGGPGATKVLCLMN 178
AGLNGMQLGDKK+IVQRAS+GAK+ + + S + GP T+VLCL+N
Sbjct: 266 AGLNGMQLGDKKLIVQRASVGAKNAQNAANTTQSVMLQVPGLSNVVTSGP-PTEVLCLLN 324
Query: 179 MXXXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFVEFESVIDC 358
M I EC+KYG V S+EIPRP +EV G GK+FVEF SV+DC
Sbjct: 325 MVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVPGCGKVFVEFNSVLDC 384
Query: 359 QKAVAALTGRKFNNRVVVTSYYDLDKYNRREF 454
QKA ALTGRKF++RVVVTSY+D DKY+RREF
Sbjct: 385 QKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 416
>sp|P26369|U2AF2_MOUSE Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
Length = 475
Score = 145 bits (365), Expect = 7e-35
Identities = 83/159 (52%), Positives = 98/159 (61%), Gaps = 9/159 (5%)
Frame = +2
Query: 5 ACAGLNGMQLGDKKIIVQRASIGAKHGIMPIMPFG-NASFESLLAVG--------GPGAT 157
A AGLNGMQLGDKK++VQRAS+GAK+ + +P N + +L G G T
Sbjct: 316 AIAGLNGMQLGDKKLLVQRASVGAKNATLVSLPSTINQTPVTLQVPGLMSSQVQMGGHPT 375
Query: 158 KVLCLMNMXXXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFVE 337
+VLCLMNM + ECSKYG V S+EIPRP +EV G GKIFVE
Sbjct: 376 EVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVE 435
Query: 338 FESVIDCQKAVAALTGRKFNNRVVVTSYYDLDKYNRREF 454
F SV DCQKA+ LTGRKF NRVVVT Y D D Y+RR+F
Sbjct: 436 FTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDF 474
>sp|P26368|U2AF2_HUMAN Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(hU2AF(65))
Length = 475
Score = 144 bits (362), Expect = 2e-34
Identities = 82/160 (51%), Positives = 97/160 (60%), Gaps = 10/160 (6%)
Frame = +2
Query: 5 ACAGLNGMQLGDKKIIVQRASIGAKHGIM----------PIMPFGNASFESLLAVGGPGA 154
A AGLNGMQLGDKK++VQRAS+GAK+ + P+ S + +GG
Sbjct: 316 AIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGH-P 374
Query: 155 TKVLCLMNMXXXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFV 334
T+VLCLMNM + ECSKYG V S+EIPRP +EV G GKIFV
Sbjct: 375 TEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFV 434
Query: 335 EFESVIDCQKAVAALTGRKFNNRVVVTSYYDLDKYNRREF 454
EF SV DCQKA+ LTGRKF NRVVVT Y D D Y+RR+F
Sbjct: 435 EFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDF 474
>sp|P90978|U2AF2_CAEEL Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 496
Score = 138 bits (347), Expect = 9e-33
Identities = 76/150 (50%), Positives = 91/150 (60%)
Frame = +2
Query: 5 ACAGLNGMQLGDKKIIVQRASIGAKHGIMPIMPFGNASFESLLAVGGPGATKVLCLMNMX 184
A AGLNGMQLGDK+++VQ A + + +A L+ G AT++LCLMNM
Sbjct: 347 AIAGLNGMQLGDKQLVVQLACANQQRHNTNLPNSASAIAGIDLSQGAGRATEILCLMNMV 406
Query: 185 XXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFVEFESVIDCQK 364
+ ECSKYG V SLEIPRP V GVGK+FVEF S DCQ+
Sbjct: 407 TEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPYEDHPVPGVGKVFVEFASTSDCQR 466
Query: 365 AVAALTGRKFNNRVVVTSYYDLDKYNRREF 454
A AALTGRKF NR VVTSYYD+DKY+ R+F
Sbjct: 467 AQAALTGRKFANRTVVTSYYDVDKYHNRQF 496
>sp|P90727|U2AF2_CAEBR Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF65)
Length = 488
Score = 136 bits (342), Expect = 3e-32
Identities = 75/150 (50%), Positives = 90/150 (60%)
Frame = +2
Query: 5 ACAGLNGMQLGDKKIIVQRASIGAKHGIMPIMPFGNASFESLLAVGGPGATKVLCLMNMX 184
A AGLNGMQLGDK+++VQ A + +A L+ G AT++LCLMNM
Sbjct: 338 AIAGLNGMQLGDKQLVVQLACANQTRHNTHLPNSASAIAGIDLSQGAGRATEILCLMNMV 397
Query: 185 XXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFVEFESVIDCQK 364
+ ECSKYG V SLEIPRP V GVGK+FVEF + DCQ+
Sbjct: 398 TEDELRSDEDYEEILEDVREECSKYGIVRSLEIPRPYDDHPVPGVGKVFVEFATTSDCQR 457
Query: 365 AVAALTGRKFNNRVVVTSYYDLDKYNRREF 454
A AALTGRKF NR VVTSYYD+DKY+ R+F
Sbjct: 458 AQAALTGRKFANRTVVTSYYDVDKYHNRQF 487
>sp|P97343|UHMK1_MOUSE Serine/threonine-protein kinase Kist (Kinase interacting with
stathmin) (U2AF homology motif kinase 1)
Length = 419
Score = 62.4 bits (150), Expect = 6e-10
Identities = 34/97 (35%), Positives = 49/97 (50%)
Frame = +2
Query: 155 TKVLCLMNMXXXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFV 334
T VL L+N+ + EC KYG V+SL +P+ NP G G++FV
Sbjct: 320 TPVLRLLNVLDDDYLENEDEYEDVVEDVKEECQKYGPVVSLLVPKENP-----GRGQVFV 374
Query: 335 EFESVIDCQKAVAALTGRKFNNRVVVTSYYDLDKYNR 445
E+ + D + A LTGR F+ + VV ++Y L Y R
Sbjct: 375 EYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKR 411
>sp|Q8TAS1|UHMK1_HUMAN Serine/threonine-protein kinase Kist (Kinase interacting with
stathmin) (U2AF homology motif kinase 1)
Length = 419
Score = 62.4 bits (150), Expect = 6e-10
Identities = 34/97 (35%), Positives = 49/97 (50%)
Frame = +2
Query: 155 TKVLCLMNMXXXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFV 334
T VL L+N+ + EC KYG V+SL +P+ NP G G++FV
Sbjct: 320 TPVLRLLNVLDDDYLENEEEYEDVVEDVKEECQKYGPVVSLLVPKENP-----GRGQVFV 374
Query: 335 EFESVIDCQKAVAALTGRKFNNRVVVTSYYDLDKYNR 445
E+ + D + A LTGR F+ + VV ++Y L Y R
Sbjct: 375 EYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKR 411
>sp|Q63285|UHMK1_RAT Serine/threonine-protein kinase Kist (Kinase interacting with
stathmin) (U2AF homology motif kinase 1) (PAM
COOH-terminal interactor protein 2) (P-CIP2)
Length = 419
Score = 62.4 bits (150), Expect = 6e-10
Identities = 34/97 (35%), Positives = 49/97 (50%)
Frame = +2
Query: 155 TKVLCLMNMXXXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVAGVGKIFV 334
T VL L+N+ + EC KYG V+SL +P+ NP G G++FV
Sbjct: 320 TPVLRLLNVLDDDYLENEDEYEDVVEDVKEECQKYGPVVSLLVPKENP-----GRGQVFV 374
Query: 335 EFESVIDCQKAVAALTGRKFNNRVVVTSYYDLDKYNR 445
E+ + D + A LTGR F+ + VV ++Y L Y R
Sbjct: 375 EYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKR 411
>sp|P36629|U2AF2_SCHPO Splicing factor U2AF 59 kDa subunit (U2 auxiliary factor 59 kDa
subunit) (U2 snRNP auxiliary factor large subunit)
(U2AF59)
Length = 517
Score = 54.7 bits (130), Expect = 1e-07
Identities = 39/154 (25%), Positives = 68/154 (44%), Gaps = 8/154 (5%)
Frame = +2
Query: 2 IACAGLNGMQLGDKKIIVQRASIGAK-------HGIMPIMPFGNASFESLLAVGGPGATK 160
+A +GL+G K+ Q A +G +G+ + AS +S+ T+
Sbjct: 366 VAISGLDGKDTYGNKLHAQFACVGLNQAMIDKSNGMAILTELAKASSQSI-------PTR 418
Query: 161 VLCLMNMXXXXXXXXXXXXXXXXXXINSECSKYGKVISLEIPRPNPSMEVA-GVGKIFVE 337
VL L N+ + ++ S YG +I ++IPR + G GK+FV
Sbjct: 419 VLQLHNLITGDEIMDVQEYEDIYESVKTQFSNYGPLIDIKIPRSIGTRNSGLGTGKVFVR 478
Query: 338 FESVIDCQKAVAALTGRKFNNRVVVTSYYDLDKY 439
+ + + A+ + G KFN+R +V ++Y D Y
Sbjct: 479 YSDIRSAEVAMEEMKGCKFNDRTIVIAFYGEDCY 512
>sp|Q8JZX4|SPF45_MOUSE Splicing factor 45 (45 kDa splicing factor) (RNA-binding motif
protein 17)
Length = 405
Score = 50.4 bits (119), Expect = 2e-06
Identities = 31/68 (45%), Positives = 37/68 (54%), Gaps = 3/68 (4%)
Frame = +2
Query: 245 ECSKYGKV---ISLEIPRPNPSMEVAGVGKIFVEFESVIDCQKAVAALTGRKFNNRVVVT 415
EC KYGKV + EIP V +IF+EFE V KAV L GR F RVV
Sbjct: 333 ECEKYGKVGKCVIFEIPGAPDDEAV----RIFLEFERVESAIKAVVDLNGRYFGGRVVKA 388
Query: 416 SYYDLDKY 439
+Y+LDK+
Sbjct: 389 CFYNLDKF 396
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,821,347
Number of Sequences: 369166
Number of extensions: 999818
Number of successful extensions: 2525
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2433
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2510
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3403581975
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)