Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_F04
(867 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 85 2e-16
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 84 5e-16
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 83 1e-15
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 82 1e-15
sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19... 82 1e-15
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 82 2e-15
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 81 4e-15
sp|Q60700|M3K12_MOUSE Mitogen-activated protein kinase kina... 80 7e-15
sp|Q63796|M3K12_RAT Mitogen-activated protein kinase kinase... 80 7e-15
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 80 7e-15
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 85.1 bits (209), Expect = 2e-16
Identities = 67/191 (35%), Positives = 100/191 (52%), Gaps = 7/191 (3%)
Frame = +2
Query: 308 DLDTPPETC---VIFQYDISRTNVKNFKMLKYEMVTRFKLPYDTSRFVQLLSMYEE--EN 472
D+DT E V F D T+ K L+ E+ L ++ R VQ + E
Sbjct: 381 DVDTGRELASKQVQFDPDSPETS-KEVSALECEIQLLKNLQHE--RIVQYYGCLRDRAEK 437
Query: 473 SVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVL 649
++ I ME M S++ LK LTE +KY+ +IL+G+SY+H I+HR + ++L
Sbjct: 438 TLTIFMEYMPGGSVKDQLKAYGA-LTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANIL 496
Query: 650 IDCNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKADIW 826
D N KL +F SK L C + ++ V G+ W++PEV+ GE YG KAD+W
Sbjct: 497 RDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPY-----WMSPEVISGEGYGRKADVW 551
Query: 827 SFGVTMLEMMT 859
S G T++EM+T
Sbjct: 552 SLGCTVVEMLT 562
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 84.0 bits (206), Expect = 5e-16
Identities = 67/191 (35%), Positives = 99/191 (51%), Gaps = 7/191 (3%)
Frame = +2
Query: 308 DLDTPPETC---VIFQYDISRTNVKNFKMLKYEMVTRFKLPYDTSRFVQLLSMYEE--EN 472
D+DT E V F D T+ K L+ E+ L ++ R VQ + E
Sbjct: 381 DVDTGRELASKQVQFDPDSPETS-KEVSALECEIQLLKNLQHE--RIVQYYGCLRDRAEK 437
Query: 473 SVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVL 649
+ I ME M S++ LK LTE +KY+ +IL+G+SY+H I+HR + ++L
Sbjct: 438 ILTIFMEYMPGGSVKDQLKAYGA-LTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANIL 496
Query: 650 IDCNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKADIW 826
D N KL +F SK L C + ++ V G+ W++PEV+ GE YG KAD+W
Sbjct: 497 RDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPY-----WMSPEVISGEGYGRKADVW 551
Query: 827 SFGVTMLEMMT 859
S G T++EM+T
Sbjct: 552 SLGCTVVEMLT 562
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 82.8 bits (203), Expect = 1e-15
Identities = 52/134 (38%), Positives = 78/134 (58%), Gaps = 2/134 (1%)
Frame = +2
Query: 464 EENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 640
+E ++ I ME M S++ LK LTE+ +KY+ +IL+G+ Y+H ILHR +
Sbjct: 428 QEKTLSIFMEYMPGGSIKDQLKAYGA-LTENGTRKYTRQILEGVHYLHSNMILHRDIKGA 486
Query: 641 SVLIDCNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKA 817
++L D N KL +F SK L C + +K V G+ W++PEV+ G+ YG KA
Sbjct: 487 NILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPY-----WMSPEVISGQGYGRKA 541
Query: 818 DIWSFGVTMLEMMT 859
DIWS T++EM+T
Sbjct: 542 DIWSVACTVVEMLT 555
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 82.4 bits (202), Expect = 1e-15
Identities = 46/142 (32%), Positives = 78/142 (54%)
Frame = +2
Query: 440 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 619
V+ L E++++ I++E + S L E ++ Y+ ++L GL Y+H I+
Sbjct: 135 VRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIM 194
Query: 620 HRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLGE 799
HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+L
Sbjct: 195 HRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPY-----WMAPEVILQT 249
Query: 800 PYGFKADIWSFGVTMLEMMTGR 865
+ F ADIWS G T++EM+TG+
Sbjct: 250 GHSFSADIWSVGCTVIEMVTGK 271
>sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19 (WRKY DNA-binding protein 19)
Length = 1895
Score = 82.4 bits (202), Expect = 1e-15
Identities = 49/140 (35%), Positives = 78/140 (55%), Gaps = 5/140 (3%)
Frame = +2
Query: 461 EEENSVFIVMERMAS-SLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNC 637
++E++++I +E + SLR L + + L + + Y+ +ILDGL Y+H +HR++ C
Sbjct: 1695 KDESNLYIFLELVTQGSLRKLYQRN--QLGDSVVSLYTRQILDGLKYLHDKGFIHRNIKC 1752
Query: 638 GSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLG----EPY 805
+VL+D N KL +F L+K + T W+APEV+L + Y
Sbjct: 1753 ANVLVDANGTVKLADFGLAKVMSLWRTPYW-------------NWMAPEVILNPKDYDGY 1799
Query: 806 GFKADIWSFGVTMLEMMTGR 865
G ADIWS G T+LEM+TG+
Sbjct: 1800 GTPADIWSLGCTVLEMLTGQ 1819
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 82.0 bits (201), Expect = 2e-15
Identities = 46/142 (32%), Positives = 77/142 (54%)
Frame = +2
Query: 440 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 619
V+ L E+ ++ I++E + S L E ++ Y+ ++L GL Y+H I+
Sbjct: 134 VRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIM 193
Query: 620 HRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLGE 799
HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+L
Sbjct: 194 HRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPY-----WMAPEVILQT 248
Query: 800 PYGFKADIWSFGVTMLEMMTGR 865
+ F ADIWS G T++EM+TG+
Sbjct: 249 GHSFSADIWSVGCTVIEMVTGK 270
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 80.9 bits (198), Expect = 4e-15
Identities = 48/142 (33%), Positives = 75/142 (52%)
Frame = +2
Query: 440 VQLLSMYEEENSVFIVMERMASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKIL 619
V+ L E +S+ I+ME + S L E I Y+ ++L GL Y+H I+
Sbjct: 134 VRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIM 193
Query: 620 HRSLNCGSVLIDCNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLGE 799
HR + ++L+D +L +F SK + + +T K +KG+ W+APEV+L
Sbjct: 194 HRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPY-----WMAPEVILQT 248
Query: 800 PYGFKADIWSFGVTMLEMMTGR 865
+ F ADIWS G T++EM TG+
Sbjct: 249 GHSFSADIWSVGCTVIEMATGK 270
>sp|Q60700|M3K12_MOUSE Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 888
Score = 80.1 bits (196), Expect = 7e-15
Identities = 53/171 (30%), Positives = 84/171 (49%)
Frame = +2
Query: 350 DISRTNVKNFKMLKYEMVTRFKLPYDTSRFVQLLSMYEEENSVFIVMERMASSLRSLLKP 529
D+ T++K+ + LK+ + FK + +L + + ++ V+ +P
Sbjct: 189 DLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAG--------RP 240
Query: 530 DTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVLIDCNDNAKLCNFHLSKFLFD 709
T L D +S I G++Y+H KI+HR L ++LI +D K+ +F SK L D
Sbjct: 241 VTPSLLVD----WSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSD 296
Query: 710 CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKADIWSFGVTMLEMMTG 862
ST+ G W+APEV+ EP K DIWSFGV + E++TG
Sbjct: 297 KSTKMSFAGTVA--------WMAPEVIRNEPVSEKVDIWSFGVVLWELLTG 339
>sp|Q63796|M3K12_RAT Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 888
Score = 80.1 bits (196), Expect = 7e-15
Identities = 53/171 (30%), Positives = 84/171 (49%)
Frame = +2
Query: 350 DISRTNVKNFKMLKYEMVTRFKLPYDTSRFVQLLSMYEEENSVFIVMERMASSLRSLLKP 529
D+ T++K+ + LK+ + FK + +L + + ++ V+ +P
Sbjct: 189 DLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAG--------RP 240
Query: 530 DTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCGSVLIDCNDNAKLCNFHLSKFLFD 709
T L D +S I G++Y+H KI+HR L ++LI +D K+ +F SK L D
Sbjct: 241 VTPSLLVD----WSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSD 296
Query: 710 CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKADIWSFGVTMLEMMTG 862
ST+ G W+APEV+ EP K DIWSFGV + E++TG
Sbjct: 297 KSTKMSFAGTVA--------WMAPEVIRNEPVSEKVDIWSFGVVLWELLTG 339
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 80.1 bits (196), Expect = 7e-15
Identities = 51/134 (38%), Positives = 77/134 (57%), Gaps = 2/134 (1%)
Frame = +2
Query: 464 EENSVFIVMERM-ASSLRSLLKPDTQHLTEDKIKKYSYEILDGLSYMHKLKILHRSLNCG 640
+E ++ I ME S++ LK LTE+ +KY+ +IL+G+ Y+H I+HR +
Sbjct: 429 QEKTLSIFMELSPGGSIKDQLKAYGA-LTENVTRKYTRQILEGVHYLHSNMIVHRDIKGA 487
Query: 641 SVLIDCNDNAKLCNFHLSKFLFD-CSTEQLLKGVKGSKIEDDECWVAPEVLLGEPYGFKA 817
++L D N KL +F SK L C + +K V G+ W++PEV+ GE YG KA
Sbjct: 488 NILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPY-----WMSPEVISGEGYGRKA 542
Query: 818 DIWSFGVTMLEMMT 859
DIWS T++EM+T
Sbjct: 543 DIWSVACTVVEMLT 556
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,673,256
Number of Sequences: 369166
Number of extensions: 2202194
Number of successful extensions: 9144
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7711
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8135
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)