Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_E18
(345 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q37376|NU2M_ACACA NADH-ubiquinone oxidoreductase chain 2... 38 0.006
sp|P02921|MELB_ECOLI Melibiose carrier protein (Thiomethylg... 32 0.35
sp|P30878|MELB_SALTY Melibiose carrier protein (Thiomethylg... 32 0.45
sp|Q8D247|THIC_WIGBR Thiamine biosynthesis protein thiC 30 1.7
sp|P16552|RAFB_ECOLI Raffinose permease 29 3.8
sp|P15577|NU2M_PARTE NADH-ubiquinone oxidoreductase chain 2... 28 5.0
sp|P26361|CFTR_MOUSE Cystic fibrosis transmembrane conducta... 28 6.6
sp|P38745|YHB7_YEAST Hypothetical 61.2 kDa protein in APM2-... 28 8.6
sp|P50076|ALG10_YEAST Alpha-1,2 glucosyltransferase ALG10 (... 28 8.6
sp|P23637|ERF2_PICPI Eukaryotic peptide chain release facto... 28 8.6
>sp|Q37376|NU2M_ACACA NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 527
Score = 38.1 bits (87), Expect = 0.006
Identities = 23/90 (25%), Positives = 40/90 (44%)
Frame = +3
Query: 48 NYFSDVVGLDNGYGFWIXXXXXXXCRFPPMLG*VLKFTVYSTILKYTILFFLFLVYLFST 227
N FS+++ ++ F I PP+LG KF ++ LKY + + L +FS
Sbjct: 403 NLFSNLLEVNPSLSFSIFILLFSIAGIPPLLGFYSKFFLFLFSLKYKMYWMTILFVIFSV 462
Query: 228 FGYIVVLRCFFVLLP*RNLFKIYFYRLPFI 317
+R ++ R I+ Y +PF+
Sbjct: 463 VSVFYYIRLVKLMYFNRTTGWIFLYDIPFL 492
>sp|P02921|MELB_ECOLI Melibiose carrier protein (Thiomethylgalactoside permease II)
(Melibiose permease) (Na+ (Li+)/melibiose symporter)
(Melibiose transporter)
Length = 469
Score = 32.3 bits (72), Expect = 0.35
Identities = 27/100 (27%), Positives = 41/100 (41%), Gaps = 12/100 (12%)
Frame = +3
Query: 3 FGISFLIYLCYSFEYNYFSDVVGLDNGY-GFWIXXXXXXXCRFPPMLG*VLKFT------ 161
FG F I + Y + Y++DVVGL G G P++G ++ T
Sbjct: 12 FGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGK 71
Query: 162 -----VYSTILKYTILFFLFLVYLFSTFGYIVVLRCFFVL 266
+ T+ ILF LF +LF IV + ++L
Sbjct: 72 FKPWILIGTLANSVILFLLFSAHLFEGTTQIVFVCVTYIL 111
>sp|P30878|MELB_SALTY Melibiose carrier protein (Thiomethylgalactoside permease II)
(Melibiose permease) (Na+ (Li+)/melibiose symporter)
(Melibiose transporter)
Length = 476
Score = 32.0 bits (71), Expect = 0.45
Identities = 25/100 (25%), Positives = 41/100 (41%), Gaps = 12/100 (12%)
Frame = +3
Query: 3 FGISFLIYLCYSFEYNYFSDVVGLDNGY-GFWIXXXXXXXCRFPPMLG*VLKFT------ 161
FG F I + Y + Y++DVVGL G G P++G ++ T
Sbjct: 16 FGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGK 75
Query: 162 -----VYSTILKYTILFFLFLVYLFSTFGYIVVLRCFFVL 266
+ T+ +LF LF +LF +V + ++L
Sbjct: 76 FKPWILIGTLTNSLVLFLLFSAHLFEGTAQVVFVCVTYIL 115
>sp|Q8D247|THIC_WIGBR Thiamine biosynthesis protein thiC
Length = 604
Score = 30.0 bits (66), Expect = 1.7
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -1
Query: 126 IYKRIKIEIISKNHNHCLNQQHRKNNYTQNYNRDISKK 13
I R +E+ISKN LN +++ NN+ +N + ISKK
Sbjct: 98 IIDRKDVELISKN----LNLENKNNNFKKNIDHYISKK 131
>sp|P16552|RAFB_ECOLI Raffinose permease
Length = 425
Score = 28.9 bits (63), Expect = 3.8
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 11/85 (12%)
Frame = +3
Query: 3 FGISFLIYL-----CYSFEYNYFSDVVGLD-NGYGFWIXXXXXXXCRFPPMLG*V----- 149
FG+ F +Y C+ F + SDVVGL G F P+LG +
Sbjct: 15 FGLFFFLYFFIMATCFPFLPVWLSDVVGLSKTDTGIVFSCLSLFAISFQPLLGVISDRLG 74
Query: 150 LKFTVYSTILKYTILFFLFLVYLFS 224
LK + +I + F F +Y+F+
Sbjct: 75 LKKNLIWSISLLLVFFAPFFLYVFA 99
>sp|P15577|NU2M_PARTE NADH-ubiquinone oxidoreductase chain 2 (NADH dehydrogenase subunit
2)
Length = 193
Score = 28.5 bits (62), Expect = 5.0
Identities = 23/101 (22%), Positives = 40/101 (39%), Gaps = 14/101 (13%)
Frame = +3
Query: 84 YGFWIXXXXXXXCRFPPMLG*VLKFTVYSTILKYTILFFLFLVYLF---STFGYIVVLRC 254
+ F++ PP+LG LKF ++ + T L F+ + F + F Y+ ++
Sbjct: 81 FKFFVLIFFLNLAGIPPLLGFFLKFLIFFFLFFKTNLAFILIFLGFNMATLFFYLSTVKS 140
Query: 255 F-----------FVLLP*RNLFKIYFYRLPFIIVFFG*FLL 344
F F L +YF+ + +FF F L
Sbjct: 141 FVNRKQASVLNSFNFFIRAELSFLYFFNFFYFFLFFAFFFL 181
>sp|P26361|CFTR_MOUSE Cystic fibrosis transmembrane conductance regulator (CFTR)
(cAMP-dependent chloride channel)
Length = 1476
Score = 28.1 bits (61), Expect = 6.6
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +3
Query: 156 FTVYSTILKYTILFFLFLVYLFSTFGYIVVLR 251
F V+ ++L YT++ + L +F+T + +VLR
Sbjct: 316 FVVFLSVLPYTVINGIVLRKIFTTISFCIVLR 347
>sp|P38745|YHB7_YEAST Hypothetical 61.2 kDa protein in APM2-DUR3 intergenic region
precursor
Length = 532
Score = 27.7 bits (60), Expect = 8.6
Identities = 16/33 (48%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = +3
Query: 147 VLKFTVYST--ILKYTILFFLFLVYLFSTFGYI 239
V+K +Y IL Y LF LFL L STF Y+
Sbjct: 367 VVKLNMYRKLIILCYISLFILFLGLLVSTFAYV 399
>sp|P50076|ALG10_YEAST Alpha-1,2 glucosyltransferase ALG10 (Alpha-2-glucosyltransferase
ALG10) (Dolichyl-phosphoglucose-dependent
glucosyltransferase ALG10) (Asparagine-linked
glycosylation protein 10)
Length = 525
Score = 27.7 bits (60), Expect = 8.6
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +3
Query: 168 STILKYTILFFLFLVYLFSTFGYIVVLR 251
S ++KY FF+ +Y FSTF Y+ V+R
Sbjct: 374 SRLIKY---FFMTPIYHFSTFAYLEVMR 398
>sp|P23637|ERF2_PICPI Eukaryotic peptide chain release factor GTP-binding subunit (ERF2)
(Translation release factor 3) (ERF3) (ERF-3)
(Omnipotent suppressor protein 2)
Length = 741
Score = 27.7 bits (60), Expect = 8.6
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -1
Query: 90 NHNHCLNQQHRKNNYTQNYNRDISKKISQ 4
N+N+ N + NNY NYN+ + SQ
Sbjct: 119 NNNYANNSYNNNNNYNNNYNQGYNNYNSQ 147
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,945,168
Number of Sequences: 369166
Number of extensions: 551420
Number of successful extensions: 1680
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1599
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1674
length of database: 68,354,980
effective HSP length: 82
effective length of database: 53,206,710
effective search space used: 1702614720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)