Planarian EST Database


Dr_sW_027_E07

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_027_E07
         (418 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q5ZIJ9|MIB2_CHICK  Ubiquitin ligase protein MIB2 (Mind bo...    38   0.007
sp|Q02989|LITA_LATMA  Alpha-latroinsectotoxin precursor (Alp...    37   0.021
sp|Q04861|NFKB1_CHICK  Nuclear factor NF-kappa-B p105 subuni...    36   0.028
sp|P19838|NFKB1_HUMAN  Nuclear factor NF-kappa-B p105 subuni...    35   0.080
sp|P25799|NFKB1_MOUSE  Nuclear factor NF-kappa-B p105 subuni...    34   0.14 
sp|Q63369|NFKB1_RAT  Nuclear factor NF-kappa-B p105 subunit ...    34   0.14 
sp|Q9VUX2|MIB_DROME  Ubiquitin ligase protein mind-bomb (Min...    33   0.18 
sp|Q68LP1|MIB2_RAT  Ubiquitin ligase protein MIB2 (Mind bomb...    33   0.18 
sp|Q05823|RN5A_HUMAN  2-5A-dependent ribonuclease (2-5A-depe...    33   0.23 
sp|Q8R516|MIB2_MOUSE  Ubiquitin ligase protein MIB2 (Mind bo...    33   0.23 
>sp|Q5ZIJ9|MIB2_CHICK Ubiquitin ligase protein MIB2 (Mind bomb homolog 2)
          Length = 954

 Score = 38.1 bits (87), Expect = 0.007
 Identities = 16/45 (35%), Positives = 29/45 (64%)
 Frame = +3

Query: 180 INMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           +N++N   ++PL+LA+I  ++ L+ + V E  D N  D +GDT +
Sbjct: 659 VNVKNNRNQTPLHLAIIQGHVGLVQLLVSEGSDVNAEDEDGDTAM 703
>sp|Q02989|LITA_LATMA Alpha-latroinsectotoxin precursor (Alpha-LIT)
          Length = 1411

 Score = 36.6 bits (83), Expect = 0.021
 Identities = 18/47 (38%), Positives = 28/47 (59%)
 Frame = +3

Query: 174  SYINMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
            S +N++NI  E P YLA+  +   + D FV +  + N  D NG+T+L
Sbjct: 861  SLMNVKNIRNEYPFYLAVEKRYKDIFDYFVSKDANVNEVDHNGNTLL 907

 Score = 28.1 bits (61), Expect = 7.5
 Identities = 12/38 (31%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
 Frame = +3

Query: 174 SYINMRNISGESPLYLALITKNLSLIDVFV-IEYVDFN 284
           +Y+N  + +G +PL+ A +T NL ++D  +  +Y++ N
Sbjct: 659 AYLNDGDANGMTPLHYAAMTGNLEMVDFLLNQQYININ 696

 Score = 27.7 bits (60), Expect = 9.8
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = +3

Query: 180 INMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           IN+++  G +PL+LA +  NLS+  +   +    N  D NG T L
Sbjct: 628 INIKSKVGLTPLHLATLQNNLSVSKLLAGKGAYLNDGDANGMTPL 672
>sp|Q04861|NFKB1_CHICK Nuclear factor NF-kappa-B p105 subunit [Contains: Nuclear factor
           NF-kappa-B p50 subunit]
          Length = 984

 Score = 36.2 bits (82), Expect = 0.028
 Identities = 18/45 (40%), Positives = 30/45 (66%)
 Frame = +3

Query: 180 INMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           INMRN   ++PL+LA+ITK   +++  +    + NL D +G++VL
Sbjct: 573 INMRNDLYQTPLHLAVITKQAEVVEDLLKAGANVNLLDRHGNSVL 617
>sp|P19838|NFKB1_HUMAN Nuclear factor NF-kappa-B p105 subunit (DNA-binding factor KBF1)
           (EBP-1) [Contains: Nuclear factor NF-kappa-B p50
           subunit]
          Length = 968

 Score = 34.7 bits (78), Expect = 0.080
 Identities = 18/48 (37%), Positives = 30/48 (62%)
 Frame = +3

Query: 171 ESYINMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           +  INMRN   ++PL+LA+ITK   +++  +    D +L D  G++VL
Sbjct: 572 DDIINMRNDLYQTPLHLAVITKQEDVVEDLLRAGADLSLLDRLGNSVL 619
>sp|P25799|NFKB1_MOUSE Nuclear factor NF-kappa-B p105 subunit (DNA-binding factor KBF1)
           (EBP-1) (NF-kappa-B1 p84/NF-kappa-B1 p98) [Contains:
           Nuclear factor NF-kappa-B p50 subunit]
          Length = 971

 Score = 33.9 bits (76), Expect = 0.14
 Identities = 18/48 (37%), Positives = 30/48 (62%)
 Frame = +3

Query: 171 ESYINMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           +  INMRN   ++PL+LA+ITK   +++  +    D +L D  G++VL
Sbjct: 568 DDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVL 615
>sp|Q63369|NFKB1_RAT Nuclear factor NF-kappa-B p105 subunit (DNA-binding factor KBF1)
           (EBP-1) [Contains: Nuclear factor NF-kappa-B p50
           subunit]
          Length = 522

 Score = 33.9 bits (76), Expect = 0.14
 Identities = 18/48 (37%), Positives = 30/48 (62%)
 Frame = +3

Query: 171 ESYINMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           +  INMRN   ++PL+LA+ITK   +++  +    D +L D  G++VL
Sbjct: 119 DDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVL 166
>sp|Q9VUX2|MIB_DROME Ubiquitin ligase protein mind-bomb (Mind bomb homolog) (D-mib)
          Length = 1226

 Score = 33.5 bits (75), Expect = 0.18
 Identities = 13/45 (28%), Positives = 31/45 (68%)
 Frame = +3

Query: 180 INMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           ++ +N++ ++ L+LA+  +++ ++ + V +  D N+PD +GDT L
Sbjct: 763 MDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPL 807
>sp|Q68LP1|MIB2_RAT Ubiquitin ligase protein MIB2 (Mind bomb homolog 2)
           (RBSC-skeletrophin/dystrophin-like polypeptide)
          Length = 971

 Score = 33.5 bits (75), Expect = 0.18
 Identities = 17/45 (37%), Positives = 26/45 (57%)
 Frame = +3

Query: 180 INMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           +N+RN   +SPL+LA+   +L L+ + V    + N  D  GDT L
Sbjct: 675 VNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCNVNTEDEEGDTAL 719
>sp|Q05823|RN5A_HUMAN 2-5A-dependent ribonuclease (2-5A-dependent RNase) (Ribonuclease L)
           (RNase L) (Ribonuclease 4)
          Length = 741

 Score = 33.1 bits (74), Expect = 0.23
 Identities = 22/75 (29%), Positives = 40/75 (53%), Gaps = 1/75 (1%)
 Frame = +3

Query: 180 INMRNISGESPLYLALITKNLSLID-VFVIEYVDFNLPDTNGDTVLCTLFKTFTNATDIP 356
           +N+R   G++PL LA+  K+L L+  +   E+++ N  D++G T L    +         
Sbjct: 232 VNVRGERGKTPLILAVEKKHLGLVQRLLEQEHIEINDTDSDGKTALLLAVEL-------- 283

Query: 357 YLEKTAKIMINGGAS 401
            L+K A+++   GAS
Sbjct: 284 KLKKIAELLCKRGAS 298
>sp|Q8R516|MIB2_MOUSE Ubiquitin ligase protein MIB2 (Mind bomb homolog 2) (Mind bomb-2)
           (Skeletrophin) (Dystrophin-like protein) (Dyslike)
          Length = 973

 Score = 33.1 bits (74), Expect = 0.23
 Identities = 17/45 (37%), Positives = 25/45 (55%)
 Frame = +3

Query: 180 INMRNISGESPLYLALITKNLSLIDVFVIEYVDFNLPDTNGDTVL 314
           +N+RN   +SPL+LA+   +L L+ + V      N  D  GDT L
Sbjct: 677 VNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTAL 721
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,055,596
Number of Sequences: 369166
Number of extensions: 722434
Number of successful extensions: 1792
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1736
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1792
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 1952582385
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)