Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_D19-2
(593 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8UVX0|PIWI_BRARE Piwi protein 133 4e-31
sp|Q9VKM1|PIWI_DROME Piwi protein 132 9e-31
sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein 89 1e-17
sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 88 1e-17
sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromo... 87 4e-17
sp|O04379|AGO1_ARATH Argonaute protein 85 1e-16
sp|Q9XGW1|PINH_ARATH PINHEAD protein (ZWILLE protein) 84 3e-16
sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor... 83 6e-16
sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation fact... 82 8e-16
sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation fact... 82 1e-15
>sp|Q8UVX0|PIWI_BRARE Piwi protein
Length = 858
Score = 133 bits (334), Expect = 4e-31
Identities = 72/200 (36%), Positives = 114/200 (57%), Gaps = 4/200 (2%)
Frame = +2
Query: 5 SISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQL 184
S++ S++ S E + L + A+ A+ + ++P R I+YRDGVGD L
Sbjct: 647 SLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGML 706
Query: 185 YLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFFK--DG--ANPNPGTVVDEK 352
+E +M+ ++ + ++ P++ VVVKKRIS +FF DG ANP PGTV+D +
Sbjct: 707 QSVVDYEVPQIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTE 765
Query: 353 IVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHL 532
+ +P +Y+F++VSQ G +PT+YNV+ D S + P +Q++TY L H+
Sbjct: 766 VTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDN----------SGLKPDHMQRLTYKLCHM 815
Query: 533 YFNWMGTIRVPVPVHYADRL 592
Y+NW G +RVP P YA +L
Sbjct: 816 YYNWQGIVRVPAPCQYAHKL 835
>sp|Q9VKM1|PIWI_DROME Piwi protein
Length = 843
Score = 132 bits (331), Expect = 9e-31
Identities = 69/192 (35%), Positives = 103/192 (53%), Gaps = 2/192 (1%)
Frame = +2
Query: 23 SQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKF 202
S Y S V + L A+ +Q + +P R + YRDGV L +F
Sbjct: 639 STYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEF 698
Query: 203 ETDAVMKMVEKIYENQNL--PQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYE 376
E +++ ++ Y L PQ+ Y+VV + ++ +FF +G NP PGT+VD+ I P Y+
Sbjct: 699 EVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYD 758
Query: 377 FYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTI 556
FYLVSQ+ +GT SPT+YNVL + ++P +Q++TY + HLY+NW GT
Sbjct: 759 FYLVSQQVRQGTVSPTSYNVLYSS----------MGLSPEKMQKLTYKMCHLYYNWSGTT 808
Query: 557 RVPVPVHYADRL 592
RVP YA +L
Sbjct: 809 RVPAVCQYAKKL 820
>sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein
Length = 1214
Score = 88.6 bits (218), Expect = 1e-17
Identities = 58/185 (31%), Positives = 89/185 (48%), Gaps = 8/185 (4%)
Frame = +2
Query: 53 KGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVE 232
+G E E++ L + +++ P I YRDGV D Q K E + + +
Sbjct: 1001 RGALEEIEDMFSITLEHLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACD 1060
Query: 233 KIYENQNLPQIIYVVVKKRISVKFFKDGA--------NPNPGTVVDEKIVKPNFYEFYLV 388
K+ P+I V+V KR +FF G N +PGTVVD IV PN +F++V
Sbjct: 1061 KVGCK---PKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMV 1117
Query: 389 SQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPV 568
S + +GTA PT YNV+ +T + +LQQ+TY+L H++ ++ P
Sbjct: 1118 SHQAIQGTAKPTRYNVIENT----------GNLDIDLLQQLTYNLCHMFPRCNRSVSYPA 1167
Query: 569 PVHYA 583
P + A
Sbjct: 1168 PAYLA 1172
>sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880
Length = 997
Score = 88.2 bits (217), Expect = 1e-17
Identities = 53/168 (31%), Positives = 82/168 (48%), Gaps = 10/168 (5%)
Frame = +2
Query: 110 AFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKR 289
AF++ G +P R I YRDGV + Q E A+ K + EN +P++ +V+V+KR
Sbjct: 790 AFRRATGQIPQRIIFYRDGVSEGQFSQVLLHEMTAIRKACNSLQENY-VPRVTFVIVQKR 848
Query: 290 ISVKFFKD----------GANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVL 439
+ F + N PGTVVD KI PN ++FYL S +GT+ P +Y+VL
Sbjct: 849 HHTRLFPEQHGNRDMTDKSGNIQPGTVVDTKICHPNEFDFYLNSHAGIQGTSRPAHYHVL 908
Query: 440 MDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 583
+D + LQ +T +L + Y ++ + P +YA
Sbjct: 909 LDE----------NGFTADQLQMLTNNLCYTYARCTKSVSIVPPAYYA 946
>sp|P34681|YO43_CAEEL Hypothetical protein ZK757.3 in chromosome III
Length = 1040
Score = 86.7 bits (213), Expect = 4e-17
Identities = 56/169 (33%), Positives = 79/169 (46%), Gaps = 8/169 (4%)
Frame = +2
Query: 104 ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVK 283
I F + P R I+YRDGV + Q + E ++ I E+ P I Y+VV+
Sbjct: 798 IITFYRHTKQKPARIIVYRDGVSEGQFSEVLREEIQSIRTACLAIAEDFR-PPITYIVVQ 856
Query: 284 KRISVKFF--------KDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVL 439
KR + F N PGT VD IV P ++FYL S +GT+ P Y+VL
Sbjct: 857 KRHHARIFCKYQNDMVGKAKNVPPGTTVDTGIVSPEGFDFYLCSHYGVQGTSRPARYHVL 916
Query: 440 MDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYAD 586
+D C +E+ Q ITY + H Y ++ +P PV+YAD
Sbjct: 917 LDE--CKFTADEI--------QSITYGMCHTYGRCTRSVSIPTPVYYAD 955
>sp|O04379|AGO1_ARATH Argonaute protein
Length = 1048
Score = 85.1 bits (209), Expect = 1e-16
Identities = 54/170 (31%), Positives = 79/170 (46%), Gaps = 10/170 (5%)
Frame = +2
Query: 104 ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVK 283
+ AF++ G P R I YRDGV + Q Y +E DA+ K + E P + +VVV+
Sbjct: 827 LIAFRRSTGHKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASL-EAGYQPPVTFVVVQ 885
Query: 284 KRISVKFFKDGANPN----------PGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYN 433
KR + F N PGTVVD KI P ++FYL S +GT+ P +Y+
Sbjct: 886 KRHHTRLFAQNHNDRHSVDRSGNILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYH 945
Query: 434 VLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 583
VL D + LQ +T +L + Y ++ + P +YA
Sbjct: 946 VLWDE----------NNFTADGLQSLTNNLCYTYARCTRSVSIVPPAYYA 985
>sp|Q9XGW1|PINH_ARATH PINHEAD protein (ZWILLE protein)
Length = 988
Score = 83.6 bits (205), Expect = 3e-16
Identities = 51/170 (30%), Positives = 80/170 (47%), Gaps = 10/170 (5%)
Frame = +2
Query: 104 ITAFQQKFGTMPHRFIIYRDGVGDSQLYLTKKFETDAVMKMVEKIYENQNLPQIIYVVVK 283
+ +F++ G P R I YRDGV + Q Y +E DA+ K + N P + ++VV+
Sbjct: 776 LISFRKATGQKPLRIIFYRDGVSEGQFYQVLLYELDAIRKACASLEPNYQ-PPVTFIVVQ 834
Query: 284 KRISVKFFKDG----------ANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYN 433
KR + F + N PGTVVD KI P ++FYL S +GT+ P +Y+
Sbjct: 835 KRHHTRLFANNHRDKNSTDRSGNILPGTVVDTKICHPTEFDFYLCSHAGIQGTSRPAHYH 894
Query: 434 VLMDTKYCNKKTNEVSPMAPSVLQQITYSLTHLYFNWMGTIRVPVPVHYA 583
VL D + +Q +T +L + Y ++ + P +YA
Sbjct: 895 VLWDE----------NNFTADGIQSLTNNLCYTYARCTRSVSIVPPAYYA 934
>sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2)
(Argonaute-2) (Golgi ER protein 95 kDa) (GERp95)
Length = 860
Score = 82.8 bits (203), Expect = 6e-16
Identities = 58/202 (28%), Positives = 92/202 (45%), Gaps = 9/202 (4%)
Frame = +2
Query: 5 SISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQL 184
S+ + ++Y + V + + E ++L + F + P R I YRDGV + Q
Sbjct: 618 SMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQF 677
Query: 185 YLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF---------KDGANPNPGT 337
E A+ + K+ E + P I ++VV+KR + F K G P GT
Sbjct: 678 QQVLHHELLAIREACIKL-EKEYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIP-AGT 735
Query: 338 VVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITY 517
VD KI P ++FYL S +GT+ P++Y+VL D + + LQ +TY
Sbjct: 736 TVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD----------NRFSSDELQILTY 785
Query: 518 SLTHLYFNWMGTIRVPVPVHYA 583
L H Y ++ +P P +YA
Sbjct: 786 QLCHTYVRCTRSVSIPAPAYYA 807
>sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3)
(Argonaute-3) (Piwi/argonaute family protein meIF2C3)
Length = 860
Score = 82.4 bits (202), Expect = 8e-16
Identities = 59/204 (28%), Positives = 93/204 (45%), Gaps = 11/204 (5%)
Frame = +2
Query: 5 SISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQL 184
S+ + S+Y + V + + E ++L + F + P R I YRDGV + Q
Sbjct: 618 SMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQF 677
Query: 185 YLTKKFETDAVMKM---VEKIYENQNLPQIIYVVVKKRISVKFF------KDGANPN--P 331
+E A+ + +EK Y+ P I Y+VV+KR + F + G + N
Sbjct: 678 RQVLYYELLAIREACISLEKDYQ----PGITYIVVQKRHHTRLFCADRTERVGRSGNIPA 733
Query: 332 GTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQI 511
GT VD I P ++FYL S +GT+ P++Y+VL D + LQ +
Sbjct: 734 GTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNF----------FTADELQLL 783
Query: 512 TYSLTHLYFNWMGTIRVPVPVHYA 583
TY L H Y ++ +P P +YA
Sbjct: 784 TYQLCHTYVRCTRSVSIPAPAYYA 807
>sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation factor 2C 1 (eIF2C 1) (eIF-2C 1)
(Argonaute-1) (Piwi/argonaute family protein meIF2C1)
Length = 857
Score = 82.0 bits (201), Expect = 1e-15
Identities = 63/202 (31%), Positives = 92/202 (45%), Gaps = 9/202 (4%)
Frame = +2
Query: 5 SISSKFSQYMSFVNSPKGKNEFHENLGKNFLTAITAFQQKFGTMPHRFIIYRDGVGDSQL 184
S+ + S+Y + V + + E E+L + F + P R I YRDGV + QL
Sbjct: 615 SMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQL 674
Query: 185 YLTKKFETDAVMKMVEKIYENQNLPQIIYVVVKKRISVKFF---------KDGANPNPGT 337
+E A+ K+ E P I Y+VV+KR + F K G P GT
Sbjct: 675 PQILHYELLAIRDACIKL-EKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIP-AGT 732
Query: 338 VVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYCNKKTNEVSPMAPSVLQQITY 517
VD I P ++FYL S +GT+ P++Y VL D N+ T + LQ +TY
Sbjct: 733 TVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDD---NRFTAD-------ELQILTY 782
Query: 518 SLTHLYFNWMGTIRVPVPVHYA 583
L H Y ++ +P P +YA
Sbjct: 783 QLCHTYVRCTRSVSIPAPAYYA 804
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,067,866
Number of Sequences: 369166
Number of extensions: 1415475
Number of successful extensions: 2980
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2948
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4504118060
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)