Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_A12
(745 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UBE0|ULE1A_HUMAN Ubiquitin-like 1-activating enzyme E1... 97 5e-20
sp|Q9R1T2|ULE1A_MOUSE Ubiquitin-like 1-activating enzyme E1... 95 2e-19
sp|P79064|RAD31_SCHPO DNA damage tolerance protein rad31 64 6e-10
sp|Q29504|UBE1_RABIT Ubiquitin-activating enzyme E1 48 3e-05
sp|P22314|UBE1_HUMAN Ubiquitin-activating enzyme E1 (A1S9 p... 46 9e-05
sp|Q06624|RHC31_YEAST DNA damage tolerance protein RHC31 (R... 46 1e-04
sp|P20973|UBE11_WHEAT Ubiquitin-activating enzyme E1 1 45 3e-04
sp|P31251|UBE12_WHEAT Ubiquitin-activating enzyme E1 2 45 3e-04
sp|P31252|UBE13_WHEAT Ubiquitin-activating enzyme E1 3 44 6e-04
sp|Q02053|UBE1_MOUSE Ubiquitin-activating enzyme E1 1 42 0.001
>sp|Q9UBE0|ULE1A_HUMAN Ubiquitin-like 1-activating enzyme E1A (SUMO-1-activating enzyme
subunit 1)
Length = 346
Score = 97.1 bits (240), Expect = 5e-20
Identities = 58/194 (29%), Positives = 101/194 (52%), Gaps = 15/194 (7%)
Frame = +1
Query: 31 INEKCRKYNKIFFGMDSFGWHGLTFMDLGRHDYVKEELAIQKSGE-----------KPNV 177
+++ C K + FF D FG+HG TF +LG H++V+E+ + K + K +
Sbjct: 142 VDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDS 201
Query: 178 NASKWIPETKQFCPLDEVLQIDWSEPRQIKKLKRIPKIQFIMLAMHKFVAEHRRYSCAN- 354
+ + + + FCP+ E L++DWS + LKR F++ + KF + R ++
Sbjct: 202 SETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDT 261
Query: 355 ---DKDDIFDIWCRFAEKSGLSSDLLNHNDFEECVGAGNVPVNSVLGGLVTQEIIKAISK 525
D + + I + G+S DLL DF + PV +V+GG++ QEI+KA+S+
Sbjct: 262 YEEDSELLLQIRNDVLDSLGISPDLL-PEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQ 320
Query: 526 KNEPVGNFVLFNGV 567
++ P NF F+G+
Sbjct: 321 RDPPHNNFFFFDGM 334
>sp|Q9R1T2|ULE1A_MOUSE Ubiquitin-like 1-activating enzyme E1A (SUMO-1-activating enzyme
subunit 1)
Length = 350
Score = 95.1 bits (235), Expect = 2e-19
Identities = 58/194 (29%), Positives = 101/194 (52%), Gaps = 15/194 (7%)
Frame = +1
Query: 31 INEKCRKYNKIFFGMDSFGWHGLTFMDLGRHDYVKEELAIQKSGE-----------KPNV 177
+++ C + + FF D FG+HG TF +LG H++V+E+ + K + K +
Sbjct: 146 VDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDS 205
Query: 178 NASKWIPETKQFCPLDEVLQIDWSEPRQIKKLKRIPKIQFIMLAMHKFVAEHRR----YS 345
+ + + + FCP+ E L++DWS + LKR F++ + KF + R S
Sbjct: 206 SETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQVLLKFRTDKGRDPTSES 265
Query: 346 CANDKDDIFDIWCRFAEKSGLSSDLLNHNDFEECVGAGNVPVNSVLGGLVTQEIIKAISK 525
D + + I + G+S DLL +DF + PV +V+GG++ QEI+KA+S+
Sbjct: 266 YKEDAELLLQIRNDVFDSLGISPDLL-PDDFVRYCFSEMAPVCAVVGGILAQEIVKALSQ 324
Query: 526 KNEPVGNFVLFNGV 567
++ P NF F+G+
Sbjct: 325 RDPPHNNFFFFDGM 338
>sp|P79064|RAD31_SCHPO DNA damage tolerance protein rad31
Length = 307
Score = 63.5 bits (153), Expect = 6e-10
Identities = 52/179 (29%), Positives = 84/179 (46%), Gaps = 2/179 (1%)
Frame = +1
Query: 31 INEKCRKYNKIFFGMDSFGWHGLTFMDLGRHDYVKEELAIQKSGEKPNVNASKWIPETKQ 210
INE R N F+ FG +G F DL H++ AI + + V +I +
Sbjct: 137 INELTRICNASFYATSCFGLYGFAFCDLINHNF-----AIDRVVDNTKVEEDMFIVQK-- 189
Query: 211 FCPLDEVLQIDWSEPRQIKKLKRIPKIQFIMLAMHKFVAEHRRYSCANDKDDIFDIWC-- 384
P+ E Q E + + K+IP + ML++ K S +D D I +
Sbjct: 190 --PMKEAFQSILGETLKPRLAKKIPTLYPAMLSLLK--------SKKSDPDSIRQVCIEQ 239
Query: 385 RFAEKSGLSSDLLNHNDFEECVGAGNVPVNSVLGGLVTQEIIKAISKKNEPVGNFVLFN 561
+ EK+ L+ + L+ F + PV SV+GG+V+Q+ + +ISKK P+ NF +F+
Sbjct: 240 KLNEKTVLNGEFLSK--FSSNISFQWTPVMSVVGGVVSQDALNSISKKQFPIDNFWIFD 296
>sp|Q29504|UBE1_RABIT Ubiquitin-activating enzyme E1
Length = 1058
Score = 47.8 bits (112), Expect = 3e-05
Identities = 29/110 (26%), Positives = 55/110 (50%), Gaps = 8/110 (7%)
Frame = +1
Query: 268 KLKRIPKIQFIMLAMHKFVAEHRRYSCANDKDDIFDIWCRFAEKSGLSSDLLNHNDFEE- 444
K R ++ A+HKF A+H R +++D ++ + +S + + +E
Sbjct: 322 KFSRPAQLHIGFQALHKFCAQHSRPPRPRNEEDAAELVTLARAVNSKASSAVQQDSLDED 381
Query: 445 ------CVGAGNV-PVNSVLGGLVTQEIIKAISKKNEPVGNFVLFNGVIC 573
V AG++ P+N+ +GGL QE++KA S K P+ ++ F+ + C
Sbjct: 382 LIRNLAFVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALEC 431
>sp|P22314|UBE1_HUMAN Ubiquitin-activating enzyme E1 (A1S9 protein)
Length = 1058
Score = 46.2 bits (108), Expect = 9e-05
Identities = 29/120 (24%), Positives = 58/120 (48%), Gaps = 8/120 (6%)
Frame = +1
Query: 268 KLKRIPKIQFIMLAMHKFVAEHRRYSCANDKDDIFDIWCRFAEKSGLSSDLLNHNDFEE- 444
K R ++ A+H+F A+H R +++D ++ + + + N+ +E
Sbjct: 322 KFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDED 381
Query: 445 ------CVGAGNV-PVNSVLGGLVTQEIIKAISKKNEPVGNFVLFNGVICSADSTFIKNE 603
V AG++ P+N+ +GGL QE++KA S K P+ ++ F+ + C + + E
Sbjct: 382 LIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTE 441
>sp|Q06624|RHC31_YEAST DNA damage tolerance protein RHC31 (RAD31 homolog)
Length = 347
Score = 45.8 bits (107), Expect = 1e-04
Identities = 45/206 (21%), Positives = 90/206 (43%), Gaps = 27/206 (13%)
Frame = +1
Query: 31 INEKCRKYNKIFFGMDSFGWHGLTFMDLGRHDYVKEELAIQ------------------- 153
IN RK N + S G F+DL +++ E+ +Q
Sbjct: 139 INTLTRKLNIPLYVAGSNGLFAYVFIDL--IEFISEDEKLQSVRPTTVGPISSNRSIIEV 196
Query: 154 --KSGEKPNVNASKWIPETKQFCPLDEVLQI-DWSEPRQIKKLKRIPKIQFIMLAMHKFV 324
+ E+ + I + PL+EVL E ++LKR+ I + L++ ++
Sbjct: 197 TTRKDEEDEKKTYERIKTKNCYRPLNEVLSTATLKEKMTQRQLKRVTSILPLTLSLLQYG 256
Query: 325 AEHRRYSCAND--KDDIFDIWCRFAEKSGLSSDLLNHNDFEECV---GAGNVPVNSVLGG 489
+ + + + K D +WC E G+ + ++ + ++ + G PV +++GG
Sbjct: 257 LNQKGKAISFEQMKRDAA-VWC---ENLGVPATVVKDDYIQQFIKQKGIEFAPVAAIIGG 312
Query: 490 LVTQEIIKAISKKNEPVGNFVLFNGV 567
V Q++I + K+ P+ NF++F+G+
Sbjct: 313 AVAQDVINILGKRLSPLNNFIVFDGI 338
>sp|P20973|UBE11_WHEAT Ubiquitin-activating enzyme E1 1
Length = 1051
Score = 44.7 bits (104), Expect = 3e-04
Identities = 41/140 (29%), Positives = 61/140 (43%), Gaps = 16/140 (11%)
Frame = +1
Query: 196 PETKQFCPLDEVLQIDWSEPRQI-----KKLKRIPKIQFIMLAMHKFVAEHRRYSCANDK 360
P+ +F PL E + SEP + K +R P + A+ KF E R+ A
Sbjct: 294 PKVIKFKPLKEAM----SEPGEFLMSDFSKFERPPLLHLAFQALDKFRTELSRFPVAGST 349
Query: 361 DDIFDIWCRFAEKSGLSSDLLNHNDFEE---------CVGAGNV--PVNSVLGGLVTQEI 507
DD+ R E + +D L EE G+ V P+ ++ GG+V QE+
Sbjct: 350 DDVQ----RVIEYAISINDTLGDRKLEEIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEV 405
Query: 508 IKAISKKNEPVGNFVLFNGV 567
+KA S K P+ F F+ V
Sbjct: 406 VKACSGKFHPLYQFFYFDSV 425
>sp|P31251|UBE12_WHEAT Ubiquitin-activating enzyme E1 2
Length = 1051
Score = 44.7 bits (104), Expect = 3e-04
Identities = 41/140 (29%), Positives = 61/140 (43%), Gaps = 16/140 (11%)
Frame = +1
Query: 196 PETKQFCPLDEVLQIDWSEPRQI-----KKLKRIPKIQFIMLAMHKFVAEHRRYSCANDK 360
P+ +F PL E + SEP + K +R P + A+ KF E R+ A
Sbjct: 294 PKVIKFKPLKEAM----SEPGEFLMSDFSKFERPPLLHLAFQALDKFRTELSRFPVAGST 349
Query: 361 DDIFDIWCRFAEKSGLSSDLLNHNDFEE---------CVGAGNV--PVNSVLGGLVTQEI 507
DD+ R E + +D L EE G+ V P+ ++ GG+V QE+
Sbjct: 350 DDVQ----RVIEYAISINDTLGDRKLEEIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEV 405
Query: 508 IKAISKKNEPVGNFVLFNGV 567
+KA S K P+ F F+ V
Sbjct: 406 VKACSGKFHPLYQFFYFDSV 425
>sp|P31252|UBE13_WHEAT Ubiquitin-activating enzyme E1 3
Length = 1053
Score = 43.5 bits (101), Expect = 6e-04
Identities = 43/179 (24%), Positives = 71/179 (39%), Gaps = 20/179 (11%)
Frame = +1
Query: 91 HGLTFMDLGRHDYVKEELAIQKSGEKPNVNASKWIP--------ETKQFCPLDEVLQIDW 246
HG+T ++ G+ VK S E+ N ++ E K C + L+
Sbjct: 252 HGMTELNDGKPRKVKNARPFSFSIEEDTSNFGIYVKGGIVTQVKEPKVLC--FKALRDAM 309
Query: 247 SEPRQI-----KKLKRIPKIQFIMLAMHKFVAEHRRY---SCANDKDDIFDIWCRFAEKS 402
++P ++ K +R P + A+ KF +H R C D I E S
Sbjct: 310 TDPGEVLLSDFSKFERPPVLHLAFQALDKFKKDHGRCPAAGCEEDAHSFLKIAAAINEAS 369
Query: 403 G-LSSDLLNHNDFEECVGAGNV---PVNSVLGGLVTQEIIKAISKKNEPVGNFVLFNGV 567
D ++ F + P+ ++ GG+V QE++KA S K P+ F F+ V
Sbjct: 370 ADRKLDTIDEKLFRQFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLNQFFYFDSV 428
>sp|Q02053|UBE1_MOUSE Ubiquitin-activating enzyme E1 1
Length = 1058
Score = 42.4 bits (98), Expect = 0.001
Identities = 28/110 (25%), Positives = 53/110 (48%), Gaps = 8/110 (7%)
Frame = +1
Query: 268 KLKRIPKIQFIMLAMHKFVAEHRRYSCANDKDDIFDIWCRFAEKSGLSSDLLNHNDFEE- 444
K R ++ A+H+F A H + +++D ++ + S + N +E
Sbjct: 322 KYSRPAQLHIGFQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDED 381
Query: 445 ------CVGAGNV-PVNSVLGGLVTQEIIKAISKKNEPVGNFVLFNGVIC 573
V AG++ P+N+ +GGL QE++KA S K P+ ++ F+ + C
Sbjct: 382 LIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALEC 431
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,067,444
Number of Sequences: 369166
Number of extensions: 1668401
Number of successful extensions: 4795
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4784
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6728100400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)