Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_A09
(648 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp||Q687E1_2 [Segment 2 of 2] Nucleotide pyrophosphatase/ph... 60 4e-09
sp|Q38924|PPAF_ARATH Iron(III)-zinc(II) purple acid phospha... 45 1e-04
sp|P80366|PPAF_PHAVU Iron(III)-zinc(II) purple acid phospha... 39 0.009
sp|Q12546|PPA_ASPFI Acid phosphatase precursor (pH 6-optimu... 35 0.22
sp|P41586|PACR_HUMAN Pituitary adenylate cyclase-activating... 34 0.37
sp|P0AC44|DHSD_ECOLI Succinate dehydrogenase hydrophobic me... 33 0.64
sp|P19274|VTPX_TTV1 Viral protein TPX 32 1.1
sp|P19275|VTP3_TTV1V Viral protein TPX 32 1.1
sp|Q8X9A9|DHSD_ECO57 Succinate dehydrogenase hydrophobic me... 31 2.4
sp|Q06502|RPOA_LDVC Replicase polyprotein 1ab (ORF1ab polyp... 30 7.0
>sp||Q687E1_2 [Segment 2 of 2] Nucleotide pyrophosphatase/phosphodiesterase
Length = 350
Score = 60.5 bits (145), Expect = 4e-09
Identities = 40/104 (38%), Positives = 53/104 (50%), Gaps = 13/104 (12%)
Frame = +3
Query: 312 WSEIHTFKTFP-PGSNWPTRYVVVGDMG-----------NLNARSLAAIQRETEK-GFYD 452
W++ +TF+ P PG N R +V GDMG N SL R E YD
Sbjct: 7 WAKPYTFRAPPTPGQNSLQRIIVFGDMGKAERDGSNEFANYQPGSLNTTDRLIEDLDNYD 66
Query: 453 MVLHVGDFAYNMDSYNAQTGDQFMRQIQPVAAQLPYMTVPGNHE 584
+V H+GD Y + Y +Q DQF Q+ P++A+ PYM GNHE
Sbjct: 67 IVFHIGDMPY-ANGYLSQW-DQFTAQVAPISAKKPYMVASGNHE 108
>sp|Q38924|PPAF_ARATH Iron(III)-zinc(II) purple acid phosphatase precursor (PAP)
Length = 469
Score = 45.4 bits (106), Expect = 1e-04
Identities = 47/186 (25%), Positives = 81/186 (43%), Gaps = 8/186 (4%)
Frame = +3
Query: 51 YNQPEQIHLSIGSNI-NXXXXXXXXXXXXNESIVIYGPDKSMNLKQ-NGTVD--KFVDGG 218
+N P+Q+H++ G++ N V Y + + KQ TV+ +F +
Sbjct: 57 HNSPQQVHVTQGNHEGNGVIISWVTPVKPGSKTVQYWCENEKSRKQAEATVNTYRFFNYT 116
Query: 219 SEKRQIFMHRVILPNLLPFTQYSYTVGSRFGWSEIHTFKTFPP--GSNWPTRYVVVGDMG 392
S ++H ++ +L T+Y Y +GS WS F PP G + P + ++GD+G
Sbjct: 117 SG----YIHHCLIDDLEFDTKYYYEIGSG-KWSRRFWF-FIPPKSGPDVPYTFGLIGDLG 170
Query: 393 NLNARSLAAIQRETEKGFYDMVLHVGDFAY--NMDSYNAQTGDQFMRQIQPVAAQLPYMT 566
+ E G VL VGD +Y +++ D + R ++ A P++
Sbjct: 171 QTYDSNSTLSHYEMNPGKGQAVLFVGDLSYADRYPNHDNNRWDTWGRFVERSVAYQPWIW 230
Query: 567 VPGNHE 584
GNHE
Sbjct: 231 TAGNHE 236
>sp|P80366|PPAF_PHAVU Iron(III)-zinc(II) purple acid phosphatase (PAP)
Length = 432
Score = 39.3 bits (90), Expect = 0.009
Identities = 47/187 (25%), Positives = 80/187 (42%), Gaps = 9/187 (4%)
Frame = +3
Query: 51 YNQPEQIHLSIGSNI-NXXXXXXXXXXXXNESIVIYGPDKSMN---LKQNGTVDKFVDGG 218
YN P+Q+H++ G + S V Y +K+ K + +F +
Sbjct: 24 YNAPQQVHITQGDLVGRAMIISWVTMDEPGSSAVRYWSEKNGRKRIAKGKMSTYRFFNYS 83
Query: 219 SEKRQIFMHRVILPNLLPFTQYSYTVGSRFGWSEIHTFKTFP-PGSNWPTRYVVVGDMGN 395
S F+H + L T+Y Y VG R + +F T P G + P + ++GD+G
Sbjct: 84 SG----FIHHTTIRKLKYNTKYYYEVGLR-NTTRRFSFITPPQTGLDVPYTFGLIGDLGQ 138
Query: 396 L--NARSLAAIQRETEKGFYDMVLHVGDFAY--NMDSYNAQTGDQFMRQIQPVAAQLPYM 563
+ +L+ + +KG VL VGD +Y +++ D + R + A P++
Sbjct: 139 SFDSNTTLSHYELSPKKG--QTVLFVGDLSYADRYPNHDNVRWDTWGRFTERSVAYQPWI 196
Query: 564 TVPGNHE 584
GNHE
Sbjct: 197 WTAGNHE 203
>sp|Q12546|PPA_ASPFI Acid phosphatase precursor (pH 6-optimum acid phosphatase) (APase6)
Length = 614
Score = 34.7 bits (78), Expect = 0.22
Identities = 30/93 (32%), Positives = 41/93 (44%), Gaps = 5/93 (5%)
Frame = +3
Query: 237 FMHRVILPNLLPFTQYSYTVGSRFG--WSEIHTFKTF-PPGSNWPTRYVVVGDMGNLNAR 407
F H V + L P T Y Y + + G SE+ +FKT P G V+ DMG NA
Sbjct: 138 FFHEVSIDGLEPDTTYYYQIPAANGTTQSEVLSFKTSRPAGHPGSFSVAVLNDMGYTNAH 197
Query: 408 SL--AAIQRETEKGFYDMVLHVGDFAYNMDSYN 500
++ TE + H GD +Y D Y+
Sbjct: 198 GTHKQLVKAATEGTAF--AWHGGDLSYADDWYS 228
>sp|P41586|PACR_HUMAN Pituitary adenylate cyclase-activating polypeptide type I receptor
precursor (PACAP type I receptor) (PACAP-R-1)
Length = 468
Score = 33.9 bits (76), Expect = 0.37
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 4/67 (5%)
Frame = +3
Query: 330 FKTFPPGSNWPTRYVVVGDMGNLNARSLA---AIQRE-TEKGFYDMVLHVGDFAYNMDSY 497
F+ F P W T + D G+ N+ L+ + R TE G+ + H D A D Y
Sbjct: 81 FRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTEDGWSEPFPHYFD-ACGFDEY 139
Query: 498 NAQTGDQ 518
++TGDQ
Sbjct: 140 ESETGDQ 146
>sp|P0AC44|DHSD_ECOLI Succinate dehydrogenase hydrophobic membrane anchor protein
sp|P0AC46|DHSD_SHIFL Succinate dehydrogenase hydrophobic membrane anchor protein
sp|P0AC45|DHSD_ECOL6 Succinate dehydrogenase hydrophobic membrane anchor protein
Length = 115
Score = 33.1 bits (74), Expect = 0.64
Identities = 13/34 (38%), Positives = 23/34 (67%), Gaps = 2/34 (5%)
Frame = +2
Query: 236 FYASSYTTEF--VAIYTIQLHCWIKIWLVRNSYI 331
F+AS++T F +A+++I +H WI +W V Y+
Sbjct: 51 FFASAFTKVFTLLALFSILIHAWIGMWQVLTDYV 84
>sp|P19274|VTPX_TTV1 Viral protein TPX
Length = 360
Score = 32.3 bits (72), Expect = 1.1
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -3
Query: 355 LEPGGKVLNV*ISDQPNLDPTV*LYCVNGNKFGSIT 248
L PG + S PN+DPT+ LY NG+ + ++T
Sbjct: 59 LSPGQSITITASSGTPNIDPTIALYYNNGSSYSNLT 94
>sp|P19275|VTP3_TTV1V Viral protein TPX
Length = 474
Score = 32.3 bits (72), Expect = 1.1
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -3
Query: 355 LEPGGKVLNV*ISDQPNLDPTV*LYCVNGNKFGSIT 248
L PG + S PN+DPT+ LY NG+ + ++T
Sbjct: 59 LSPGQSITITASSGTPNIDPTIALYYNNGSSYSNLT 94
>sp|Q8X9A9|DHSD_ECO57 Succinate dehydrogenase hydrophobic membrane anchor protein
Length = 115
Score = 31.2 bits (69), Expect = 2.4
Identities = 13/34 (38%), Positives = 22/34 (64%), Gaps = 2/34 (5%)
Frame = +2
Query: 236 FYASSYTTEF--VAIYTIQLHCWIKIWLVRNSYI 331
F AS++T F +A+++I +H WI +W V Y+
Sbjct: 51 FCASAFTKVFTLLALFSILIHAWIGMWQVLTDYV 84
>sp|Q06502|RPOA_LDVC Replicase polyprotein 1ab (ORF1ab polyprotein) [Includes: Replicase
polyprotein 1a (ORF1a)] [Contains: Nsp1-alpha
papain-like cysteine proteinase (PCP1-alpha); Nsp1-beta
papain-like cysteine proteinase (PCP1-beta); Nsp2
cysteine proteinase (CP2) (CP); Nonstructural protein 3
(Nsp3); 3C-like serine proteinase (3.4.21.-) (3CLSP)
(Nsp4); Nonstructural protein 5-6-7 (Nsp5-6-7);
Nonstructural protein 8 (Nsp8); RNA-directed RNA
polymerase (RdRp) (Pol) (Nsp9); Helicase (Hel) (Nsp10);
Nonstructural protein 11 (Nsp11); Nonstructural protein
12 (Nsp12)]
Length = 3637
Score = 29.6 bits (65), Expect = 7.0
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +3
Query: 564 TVPGNHEEAYNFSNYRRRFTMPGGDGEG 647
++P +H NFSNY ++ GGDG G
Sbjct: 226 SLPADHLVKINFSNYPFDYSFTGGDGAG 253
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,274,252
Number of Sequences: 369166
Number of extensions: 1585661
Number of successful extensions: 3064
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3000
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3062
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5316264630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)