Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_A06
(383 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q19529|CMC3_CAEEL Probable calcium-binding mitochondrial... 91 6e-19
sp|Q20799|CMC2_CAEEL Probable calcium-binding mitochondrial... 85 4e-17
sp|P48233|CMC1_YEAST Calcium-binding mitochondrial carrier ... 69 4e-12
sp|P40556|YIA6_YEAST Putative mitochondrial carrier YIL006W 59 4e-09
sp|P40464|FLX1_YEAST Mitochondrial FAD carrier protein FLX1 54 8e-08
sp|P16261|GDC_RAT Grave'S disease carrier protein (GDC) (Mi... 54 1e-07
sp|Q01888|GDC_BOVIN Grave's disease carrier protein (GDC) (... 52 3e-07
sp|Q10248|YD1K_SCHPO Putative mitochondrial carrier C4G9.20c 51 7e-07
sp|O13805|YE08_SCHPO Putative mitochondrial carrier C17H9.08 51 9e-07
sp|P38127|RIM2_YEAST Mitochondrial carrier protein RIM2 50 1e-06
>sp|Q19529|CMC3_CAEEL Probable calcium-binding mitochondrial carrier F17E5.2
Length = 531
Score = 91.3 bits (225), Expect = 6e-19
Identities = 51/119 (42%), Positives = 74/119 (62%), Gaps = 4/119 (3%)
Frame = +2
Query: 2 YAGIELTLYEKCKSLYMHWANVPVAGHPHPLVIPLMS--SFSATCGILTTYPFALIRTKL 175
YAGI+LT+YE KS+Y + H P V+ L++ + S+TCG L +YP AL+RT+L
Sbjct: 410 YAGIDLTVYESLKSMYTKY----YTEHTEPGVLALLACGTCSSTCGQLASYPLALVRTRL 465
Query: 176 QSK--SEFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASSISYLTYEHLNR 346
Q++ S + ++ G + I T GF GLYRGI+ N KV+PA SISY+ YE + +
Sbjct: 466 QARAISPKNSTQPDTMVGQFKHILQTEGFTGLYRGITPNFMKVIPAVSISYVVYEKVRK 524
Score = 32.0 bits (71), Expect = 0.45
Identities = 17/69 (24%), Positives = 31/69 (44%)
Frame = +2
Query: 146 YPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASSISYL 325
YP +++T+L + + Q F +Y+ G + Y+G NL ++P + I
Sbjct: 359 YPMEVMKTRLALRR--TGQLDKGMFHFAHKMYTKEGIKCFYKGYLPNLLGIIPYAGIDLT 416
Query: 326 TYEHLNRSF 352
YE L +
Sbjct: 417 VYESLKSMY 425
>sp|Q20799|CMC2_CAEEL Probable calcium-binding mitochondrial carrier F55A11.4
Length = 588
Score = 85.1 bits (209), Expect = 4e-17
Identities = 48/113 (42%), Positives = 68/113 (60%), Gaps = 2/113 (1%)
Frame = +2
Query: 2 YAGIELTLYEKCKSLYMHWANVPVAGHPHPLVIPLMSSFSATCGILTTYPFALIRTKLQS 181
YAGI+L +YE K Y+ + + P L + + S+TCG L++YPFAL+RT+LQ+
Sbjct: 414 YAGIDLAIYETLKRTYVRYYETN-SSEPGVLALLACGTCSSTCGQLSSYPFALVRTRLQA 472
Query: 182 KS--EFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASSISYLTYE 334
S +S Q ++ FG + I G G YRGI+ N KV+PA SISY+ YE
Sbjct: 473 LSITRYSPQP-DTMFGQFKYILQNEGVTGFYRGITPNFLKVIPAVSISYVVYE 524
Score = 35.0 bits (79), Expect = 0.053
Identities = 19/71 (26%), Positives = 33/71 (46%)
Frame = +2
Query: 140 TTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASSIS 319
T YP +++T+L + + Q +Y+ G R Y+G NL ++P + I
Sbjct: 361 TIYPMEVMKTRLALRK--TGQLDRGIIHFAHKMYTKEGIRCFYKGYLPNLIGIIPYAGID 418
Query: 320 YLTYEHLNRSF 352
YE L R++
Sbjct: 419 LAIYETLKRTY 429
>sp|P48233|CMC1_YEAST Calcium-binding mitochondrial carrier SAL1 (Suppressor of AAC2
lethality)
Length = 545
Score = 68.6 bits (166), Expect = 4e-12
Identities = 37/92 (40%), Positives = 57/92 (61%), Gaps = 2/92 (2%)
Frame = +2
Query: 92 LVIPLMSSFSATCGILTTYPFALIRTKLQSKSEFSEQNINSAFG--LLRTIYSTAGFRGL 265
LV+ M +FS T G YP L+RT+LQ++ ++ + + F LL+T+ G++GL
Sbjct: 455 LVVLPMGAFSGTVGASVVYPINLLRTRLQAQGTYAHPYVYNGFKDVLLKTL-EREGYQGL 513
Query: 266 YRGISANLCKVLPASSISYLTYEHLNRSFNLK 361
++G+ L KV PA SISYL YE+L + NL+
Sbjct: 514 FKGLVPTLAKVCPAVSISYLCYENLKKFMNLE 545
>sp|P40556|YIA6_YEAST Putative mitochondrial carrier YIL006W
Length = 373
Score = 58.5 bits (140), Expect = 4e-09
Identities = 28/88 (31%), Positives = 50/88 (56%), Gaps = 1/88 (1%)
Frame = +2
Query: 104 LMSSFSATCGILTTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISA 283
+ SS S TYP ++RT++Q KS+ + F L++ Y+ G +G Y G +
Sbjct: 283 MASSVSKMIASAVTYPHEILRTRMQLKSDIPDSIQRRLFPLIKATYAQEGLKGFYSGFTT 342
Query: 284 NLCKVLPASSISYLTYEHL-NRSFNLKS 364
NL + +PAS+I+ +++E+ NR N+ +
Sbjct: 343 NLVRTIPASAITLVSFEYFRNRLENIST 370
Score = 34.3 bits (77), Expect = 0.090
Identities = 22/90 (24%), Positives = 38/90 (42%), Gaps = 3/90 (3%)
Frame = +2
Query: 95 VIPLMSSFSATCGILTTYPFALIRTKLQSK---SEFSEQNINSAFGLLRTIYSTAGFRGL 265
+ L +F+ + P + +T+LQ++ + F G L TI G RGL
Sbjct: 79 ITALSGAFAGFLSGVAVCPLDVAKTRLQAQGLQTRFENPYYRGIMGTLSTIVRDEGPRGL 138
Query: 266 YRGISANLCKVLPASSISYLTYEHLNRSFN 355
Y+G+ + P I + YE + F+
Sbjct: 139 YKGLVPIVLGYFPTWMIYFSVYEFSKKFFH 168
Score = 31.6 bits (70), Expect = 0.59
Identities = 23/100 (23%), Positives = 42/100 (42%), Gaps = 10/100 (10%)
Frame = +2
Query: 95 VIPLMSSFSATCGILT--------TYPFALIRTKLQSKSEFSEQ--NINSAFGLLRTIYS 244
+ P + +C +T T P +++T+L +S E + F R ++
Sbjct: 170 IFPQFDFVAQSCAAITAGAASTTLTNPIWVVKTRLMLQSNLGEHPTHYKGTFDAFRKLFY 229
Query: 245 TAGFRGLYRGISANLCKVLPASSISYLTYEHLNRSFNLKS 364
GF+ LY G+ +L + +I + YE L F+ S
Sbjct: 230 QEGFKALYAGLVPSLLGLFHV-AIHFPIYEDLKVRFHCYS 268
>sp|P40464|FLX1_YEAST Mitochondrial FAD carrier protein FLX1
Length = 311
Score = 54.3 bits (129), Expect = 8e-08
Identities = 28/78 (35%), Positives = 47/78 (60%)
Frame = +2
Query: 107 MSSFSATCGILTTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISAN 286
++S + YPF L+++ LQS +EQ F L++ I + GF GLY+G+SAN
Sbjct: 232 ITSLGKMVSVTLVYPFQLLKSNLQS-FRANEQKFR-LFPLIKLIIANDGFVGLYKGLSAN 289
Query: 287 LCKVLPASSISYLTYEHL 340
L + +P++ I++ YE+L
Sbjct: 290 LVRAIPSTCITFCVYENL 307
Score = 35.4 bits (80), Expect = 0.041
Identities = 19/70 (27%), Positives = 40/70 (57%), Gaps = 3/70 (4%)
Frame = +2
Query: 77 GHPHPLVIPLMSSFSATCGILT---TYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYST 247
G+ H + + S A+ G++T T P +I+T++ S S+ ++ S + ++ + T
Sbjct: 118 GNDHKMNSLIYLSAGASSGLMTAILTNPIWVIKTRIMSTSKGAQGAYTSMYNGVQQLLRT 177
Query: 248 AGFRGLYRGI 277
GF+GL++G+
Sbjct: 178 DGFQGLWKGL 187
>sp|P16261|GDC_RAT Grave'S disease carrier protein (GDC) (Mitochondrial solute carrier
protein homolog)
Length = 322
Score = 53.9 bits (128), Expect = 1e-07
Identities = 39/128 (30%), Positives = 62/128 (48%), Gaps = 3/128 (2%)
Frame = +2
Query: 2 YAGIELTLYEKCKSLYMHWANVPVAGHPHPLVIPLMSSFSATCGILTTYPFALIRTKL-- 175
Y I+ +E K+ + V+GH H L+ M+ + ++ TYP ++R +L
Sbjct: 106 YGAIQFMAFEHYKTFIT--TKLGVSGHVHRLMAGSMAGKMSMTAVICTYPLDVVRVRLAF 163
Query: 176 QSKSEFSEQNINSAFGLLRTIYST-AGFRGLYRGISANLCKVLPASSISYLTYEHLNRSF 352
Q K E + I AF +TIY+ GF G YRG+ + + P +S+S+ T+
Sbjct: 164 QVKGEHTYSGIIHAF---KTIYAKEGGFLGFYRGLMPTILGMAPYASVSFFTFG------ 214
Query: 353 NLKSFGSS 376
LKS G S
Sbjct: 215 TLKSVGLS 222
Score = 38.5 bits (88), Expect = 0.005
Identities = 29/119 (24%), Positives = 58/119 (48%), Gaps = 12/119 (10%)
Frame = +2
Query: 2 YAGIELTLYEKCKSLYMHWANVPVAGHP---HPLVIPLMSSFSATCGILT-------TYP 151
YA + + KS+ + +A + G P +P V+ L + + CG + +YP
Sbjct: 205 YASVSFFTFGTLKSVGLSYAPT-LLGRPSSDNPNVLVLKTHINLLCGGVARAIAQTISYP 263
Query: 152 FALIRTKLQSKSEFSE-QNINSAFGLLRTIYSTAGFR-GLYRGISANLCKVLPASSISY 322
F + R ++Q + E + + ++ +Y G R GLYRG+S N + +P+ ++++
Sbjct: 264 FDVTRRRMQLGAVLPEFEKCLTMRETMKYVYGHHGIRRGLYRGLSLNYIRCIPSQAVAF 322
Score = 37.4 bits (85), Expect = 0.011
Identities = 19/78 (24%), Positives = 35/78 (44%)
Frame = +2
Query: 104 LMSSFSATCGILTTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISA 283
L S + C T P ++ LQ+ + + LR + G+ GLY+G A
Sbjct: 41 LAGSIAGCCAKTTVAPLDRVKVLLQAHNHHYKHL--GVLSPLRAVPQKEGYLGLYKGNGA 98
Query: 284 NLCKVLPASSISYLTYEH 337
+ ++ P +I ++ +EH
Sbjct: 99 MMIRIFPYGAIQFMAFEH 116
>sp|Q01888|GDC_BOVIN Grave's disease carrier protein (GDC) (Mitochondrial solute carrier
protein homolog)
Length = 330
Score = 52.4 bits (124), Expect = 3e-07
Identities = 40/128 (31%), Positives = 63/128 (49%), Gaps = 3/128 (2%)
Frame = +2
Query: 2 YAGIELTLYEKCKSLYMHWANVPVAGHPHPLVIPLMSSFSATCGILTTYPFALIRTKL-- 175
Y I+ +E K+L + V+GH H L+ M+ +A ++ TYP ++R +L
Sbjct: 104 YGAIQFMAFEHYKTLIT--TKLGVSGHVHRLMAGSMAGMTA---VICTYPLDMVRVRLAF 158
Query: 176 QSKSEFSEQNINSAFGLLRTIYST-AGFRGLYRGISANLCKVLPASSISYLTYEHLNRSF 352
Q K E + I AF +TIY+ GF G YRG+ + + P + +S+ T+
Sbjct: 159 QVKGEHTYTGIIHAF---KTIYAKEGGFLGFYRGLMPTILGMAPYAGVSFFTFG------ 209
Query: 353 NLKSFGSS 376
LKS G S
Sbjct: 210 TLKSVGLS 217
Score = 50.1 bits (118), Expect = 2e-06
Identities = 35/131 (26%), Positives = 67/131 (51%), Gaps = 12/131 (9%)
Frame = +2
Query: 2 YAGIELTLYEKCKSLYMHWANVPVAGHP---HPLVIPLMSSFSATCGILT-------TYP 151
YAG+ + KS+ + +A + G P +P V+ L + + CG + +YP
Sbjct: 200 YAGVSFFTFGTLKSVGLSYAPT-LLGRPSSDNPNVLVLKTHINLLCGGVAGAIAQTISYP 258
Query: 152 FALIRTKLQSKSEFSE-QNINSAFGLLRTIYSTAGFR-GLYRGISANLCKVLPASSISYL 325
F + R ++Q + E + + ++ +Y G R GLYRG+S N + +P+ ++++
Sbjct: 259 FDVTRRRMQLGAVLPEFEKCLTMRETMKYVYGHHGIRKGLYRGLSLNYIRCVPSQAVAFT 318
Query: 326 TYEHLNRSFNL 358
TYE + + F+L
Sbjct: 319 TYELMKQFFHL 329
Score = 38.5 bits (88), Expect = 0.005
Identities = 19/78 (24%), Positives = 34/78 (43%)
Frame = +2
Query: 104 LMSSFSATCGILTTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISA 283
L + C T P ++ LQ+ + F LR + G+ GLY+G A
Sbjct: 39 LAGGIAGCCAKTTVAPLDRVKVLLQAHNHHYRHL--GVFSTLRAVPKKEGYLGLYKGNGA 96
Query: 284 NLCKVLPASSISYLTYEH 337
+ ++ P +I ++ +EH
Sbjct: 97 MMIRIFPYGAIQFMAFEH 114
>sp|Q10248|YD1K_SCHPO Putative mitochondrial carrier C4G9.20c
Length = 302
Score = 51.2 bits (121), Expect = 7e-07
Identities = 21/70 (30%), Positives = 42/70 (60%)
Frame = +2
Query: 137 LTTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASSI 316
L YPF ++++K+Q+ S+ +++ + IY+ AG RG YRG L + PA+++
Sbjct: 228 LAAYPFDIVKSKIQTDGFLSKATYKNSWQCAKGIYTKAGLRGFYRGFVPVLVRAAPANAV 287
Query: 317 SYLTYEHLNR 346
++ YE +++
Sbjct: 288 TFYVYETVSQ 297
Score = 35.0 bits (79), Expect = 0.053
Identities = 21/74 (28%), Positives = 35/74 (47%)
Frame = +2
Query: 134 ILTTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASS 313
+L PF ++ +LQS+S S N+A ++ I G Y+G L + S
Sbjct: 32 VLVGQPFDCVKVRLQSQSNVSPI-YNNALDCVKKISKNEGLAAFYKGTVLPLLGIGFCVS 90
Query: 314 ISYLTYEHLNRSFN 355
I + T+E+ R F+
Sbjct: 91 IQFTTFEYCKRFFS 104
>sp|O13805|YE08_SCHPO Putative mitochondrial carrier C17H9.08
Length = 326
Score = 50.8 bits (120), Expect = 9e-07
Identities = 24/78 (30%), Positives = 39/78 (50%)
Frame = +2
Query: 104 LMSSFSATCGILTTYPFALIRTKLQSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISA 283
L +F+ CG +YPF + R K+Q ++ +++T Y AG RG + G++
Sbjct: 241 LCGAFAGVCGQTVSYPFEVCRRKMQIGGIRKNKSFLRLKQVVQTTYKEAGMRGFFVGLTI 300
Query: 284 NLCKVLPASSISYLTYEH 337
KV+P S S+ Y H
Sbjct: 301 GYIKVIPMVSTSFFVYNH 318
Score = 39.3 bits (90), Expect = 0.003
Identities = 33/127 (25%), Positives = 51/127 (40%), Gaps = 16/127 (12%)
Frame = +2
Query: 2 YAGIELTLYEKCKSLYMHWANVPVAGHPHPLVIPLMSSFSATCGILTTYPFALIRTKLQ- 178
YAGI+ YE+ + + + H L S + TC + TYP LIR +L
Sbjct: 92 YAGIKFVAYEQVRRVLIRDPE-----HETHARRFLSGSLAGTCSVFFTYPLELIRVRLAY 146
Query: 179 -------------SKSEFSEQNI--NSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASS 313
+K F E++ N + L + F YRG S L + P +
Sbjct: 147 ITNTGKNPTLTQVTKDIFHERDFLCNKKYPGLSRLSKICNF---YRGFSVTLTGIFPYAG 203
Query: 314 ISYLTYE 334
+S+L Y+
Sbjct: 204 MSFLAYD 210
Score = 36.2 bits (82), Expect = 0.024
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +2
Query: 227 LRTIYSTAGFRGLYRGISANLCKVLPASSISYLTYEHLNR 346
++ IY G GLY+G +A L +V P + I ++ YE + R
Sbjct: 66 IKHIYHVYGLHGLYQGHTATLYRVFPYAGIKFVAYEQVRR 105
>sp|P38127|RIM2_YEAST Mitochondrial carrier protein RIM2
Length = 377
Score = 50.4 bits (119), Expect = 1e-06
Identities = 32/129 (24%), Positives = 54/129 (41%), Gaps = 27/129 (20%)
Frame = +2
Query: 26 YEKCKSLYMHWANVPVAGHP--HPLVIPLMSSFSATCGILTTYPFALIRTKLQSK---SE 190
Y++ + ++ + HP P V + G + T PF L++T+LQS
Sbjct: 29 YKEATQIPLNLKQSEIENHPTVKPWVHFVAGGIGGMAGAVVTCPFDLVKTRLQSDIFLKA 88
Query: 191 FSEQNIN----------------------SAFGLLRTIYSTAGFRGLYRGISANLCKVLP 304
+ Q +N G++ +Y GFR L++G+ NL V+P
Sbjct: 89 YKSQAVNISKGSTRPKSINYVIQAGTHFKETLGIIGNVYKQEGFRSLFKGLGPNLVGVIP 148
Query: 305 ASSISYLTY 331
A SI++ TY
Sbjct: 149 ARSINFFTY 157
Score = 37.4 bits (85), Expect = 0.011
Identities = 28/120 (23%), Positives = 54/120 (45%), Gaps = 2/120 (1%)
Frame = +2
Query: 11 IELTLYEKCKSLYMHWANVPVAGHPHPLVIPLMSSFSATCGILTTYPFALIRTKLQ--SK 184
I Y K +Y N G P++ + ++ + T P LI+T++Q
Sbjct: 152 INFFTYGTTKDMYAKAFN---NGQETPMIHLMAAATAGWATATATNPIWLIKTRVQLDKA 208
Query: 185 SEFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASSISYLTYEHLNRSFNLKS 364
+ S + +++ L+++ GF GLY+G+SA+ + + +L YE + R +S
Sbjct: 209 GKTSVRQYKNSWDCLKSVIRNEGFTGLYKGLSASYLGSV-EGILQWLLYEQMKRLIKERS 267
Score = 35.0 bits (79), Expect = 0.053
Identities = 18/67 (26%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = +2
Query: 137 LTTYPFALIRTKL-QSKSEFSEQNINSAFGLLRTIYSTAGFRGLYRGISANLCKVLPASS 313
+ TYP ++RT+L Q+ E ++ + I G +Y G++ +L + +P S
Sbjct: 304 IATYPHEVVRTRLRQTPKENGKRKYTGLVQSFKVIIKEEGLFSMYSGLTPHLMRTVPNSI 363
Query: 314 ISYLTYE 334
I + T+E
Sbjct: 364 IMFGTWE 370
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,022,843
Number of Sequences: 369166
Number of extensions: 859950
Number of successful extensions: 2513
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2376
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2501
length of database: 68,354,980
effective HSP length: 94
effective length of database: 50,989,890
effective search space used: 1682666370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)