Planarian EST Database


Dr_sW_026_P21

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_026_P21
         (788 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P20287|G3P_SCHMA  Glyceraldehyde-3-phosphate dehydrogenas...   362   e-100
sp|Q9N2D5|G3P_FELCA  Glyceraldehyde-3-phosphate dehydrogenas...   361   1e-99
sp|P51469|G3P_XENLA  Glyceraldehyde-3-phosphate dehydrogenas...   361   1e-99
sp|O57479|G3P_COLLI  Glyceraldehyde-3-phosphate dehydrogenas...   360   3e-99
sp|P00355|G3P_PIG  Glyceraldehyde-3-phosphate dehydrogenase ...   359   5e-99
sp|Q05025|G3P_COTJA  Glyceraldehyde-3-phosphate dehydrogenas...   358   7e-99
sp|Q28554|G3P_SHEEP  Glyceraldehyde-3-phosphate dehydrogenas...   358   9e-99
sp|Q28259|G3P_CANFA  Glyceraldehyde-3-phosphate dehydrogenas...   358   1e-98
sp|P00356|G3P_CHICK  Glyceraldehyde-3-phosphate dehydrogenas...   357   3e-98
sp|P46406|G3P_RABIT  Glyceraldehyde-3-phosphate dehydrogenas...   356   5e-98
>sp|P20287|G3P_SCHMA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Major larval
           surface antigen) (P-37)
          Length = 338

 Score =  362 bits (928), Expect = e-100
 Identities = 177/244 (72%), Positives = 200/244 (81%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTTI+KA AH KNNRAKKV+ISAPSADAPMFV+GVN   Y+ S+S+VSNASC
Sbjct: 94  YVVESTGVFTTIDKAQAHIKNNRAKKVIISAPSADAPMFVVGVNENSYEKSMSVVSNASC 153

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++D F I EGLMTT+H++TATQK VDGPSSKLWRDGRGA QNIIP    
Sbjct: 154 TTNCLAPLAKVIHDKFEIVEGLMTTVHSFTATQKVVDGPSSKLWRDGRGAMQNIIPASTG 213

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IP LNGK+TGMAFRVP PDVSVVDLTC+L K A Y+EIKAA+K A+   +KG
Sbjct: 214 AAKAVGKVIPALNGKLTGMAFRVPTPDVSVVDLTCRLGKGASYEEIKAAVKAAASGPLKG 273

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE+EVVS DF G  +SSIFDAKAGI L + FVKLV+WYDNE+GYSCRV+DLI HM 
Sbjct: 274 ILEYTEDEVVSSDFVGSTSSSIFDAKAGISLNNNFVKLVSWYDNEFGYSCRVVDLITHMH 333

Query: 731 TKDH 742
             DH
Sbjct: 334 KVDH 337
>sp|Q9N2D5|G3P_FELCA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  361 bits (927), Expect = 1e-99
 Identities = 179/243 (73%), Positives = 203/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AK+V+ISAPSADAPMFVMGVNHEKYD SL IVSNASC
Sbjct: 92  YVVESTGVFTTMEKAGAHLKGG-AKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP    
Sbjct: 151 TTNCLAPLAKVIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+KAAKYD+IK  +K ASE  +KG
Sbjct: 211 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE++VVS DF  D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM+
Sbjct: 271 ILGYTEDQVVSCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
>sp|P51469|G3P_XENLA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  361 bits (927), Expect = 1e-99
 Identities = 181/243 (74%), Positives = 204/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT EKAS H K   AK+VVISAPSADAPMFV+GVNHEKY+ SL +VSNASC
Sbjct: 92  YVVESTGVFTTTEKASLHLKGG-AKRVVISAPSADAPMFVVGVNHEKYENSLKVVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV+ND+FGI EGLMTT+HA+TATQKTVDGPS KLWRDGRGA QNIIP    
Sbjct: 151 TTNCLAPLAKVINDNFGIVEGLMTTVHAFTATQKTVDGPSGKLWRDGRGAGQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGKITGMAFRVP P+VSVVDLTC+L+K AKYD+IKAAIK ASE  MKG
Sbjct: 211 AAKAVGKVIPELNGKITGMAFRVPTPNVSVVDLTCRLQKPAKYDDIKAAIKTASEGPMKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YT+++VVS DF GD +SSIFDA AGI L + FVKLV+WYDNE GYS RV+DL+ HM+
Sbjct: 271 ILGYTQDQVVSTDFNGDTHSSIFDADAGIALNENFVKLVSWYDNECGYSNRVVDLVCHMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
>sp|O57479|G3P_COLLI Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  360 bits (923), Expect = 3e-99
 Identities = 179/243 (73%), Positives = 204/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AK+V+ISAPSADAPMFVMGVNHEKYD SL IVSNASC
Sbjct: 92  YVVESTGVFTTMEKAGAHLKGG-AKRVIISAPSADAPMFVMGVNHEKYDKSLKIVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++D+FGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP    
Sbjct: 151 TTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYD+IK  +K A++  +KG
Sbjct: 211 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVVKAAADGPLKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           ILAYTE++VVS DF GD +SS FDA AGI L D FVKLV+WYDNEYGYS RV+DL+ HM+
Sbjct: 271 ILAYTEDQVVSCDFNGDSHSSTFDAGAGIALNDHFVKLVSWYDNEYGYSNRVVDLMVHMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
>sp|P00355|G3P_PIG Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  359 bits (921), Expect = 5e-99
 Identities = 178/243 (73%), Positives = 202/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AK+V+ISAPSADAPMFVMGVNHEKYD SL IVSNASC
Sbjct: 92  YVVESTGVFTTMEKAGAHLKGG-AKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP    
Sbjct: 151 TTNCLAPLAKVIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYD+IK  +K ASE  +KG
Sbjct: 211 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE++VVS DF  D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM+
Sbjct: 271 ILGYTEDQVVSCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVHMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
>sp|Q05025|G3P_COTJA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  358 bits (920), Expect = 7e-99
 Identities = 178/243 (73%), Positives = 203/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AK+V+ISAPSADAPMFVMGVNHEKYD SL IVSNASC
Sbjct: 92  YVVESTGVFTTMEKAGAHLKGG-AKRVIISAPSADAPMFVMGVNHEKYDKSLKIVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++D+FGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP    
Sbjct: 151 TTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYD+IK  +K A+E  +KG
Sbjct: 211 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVVKAAAEGPLKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE++VVS DF GD +SS FDA AGI L D FVKLV+WYDNE+GYS RV+DL+ HM+
Sbjct: 271 ILGYTEDQVVSCDFNGDSHSSTFDAGAGIALNDNFVKLVSWYDNEFGYSNRVVDLMVHMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
>sp|Q28554|G3P_SHEEP Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 322

 Score =  358 bits (919), Expect = 9e-99
 Identities = 178/243 (73%), Positives = 202/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AKKV+ISAPSADAPMFVMGVNHEKY+ +L IVSNASC
Sbjct: 81  YVVESTGVFTTMEKAGAHLKGG-AKKVIISAPSADAPMFVMGVNHEKYNNTLKIVSNASC 139

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP    
Sbjct: 140 TTNCLAPLAKVIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTG 199

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYDEIK  +K ASE  +KG
Sbjct: 200 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDEIKKVVKQASEGPLKG 259

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE++VVS DF  D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM+
Sbjct: 260 ILGYTEDQVVSCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVHMA 319

Query: 731 TKD 739
           +K+
Sbjct: 320 SKE 322
>sp|Q28259|G3P_CANFA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  358 bits (918), Expect = 1e-98
 Identities = 177/243 (72%), Positives = 203/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AK+V+ISAPSADAPMFVMGVNHEKYD SL IVSNASC
Sbjct: 92  YVVESTGVFTTMEKAGAHLKGG-AKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++DHFGI EGLMTT+HA TATQKTVDGPS K+WRDGRGAAQNIIP    
Sbjct: 151 TTNCLAPLAKVIHDHFGIVEGLMTTVHAITATQKTVDGPSGKMWRDGRGAAQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+KAAKYD+IK  +K ASE  +KG
Sbjct: 211 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE++VVS DF  D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ +M+
Sbjct: 271 ILGYTEDQVVSCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVYMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
>sp|P00356|G3P_CHICK Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  357 bits (915), Expect = 3e-98
 Identities = 177/243 (72%), Positives = 203/243 (83%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AK+V+ISAPSADAPMFVMGVNHEKYD SL IVSNASC
Sbjct: 92  YVVESTGVFTTMEKAGAHLKGG-AKRVIISAPSADAPMFVMGVNHEKYDKSLKIVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++D+FGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP    
Sbjct: 151 TTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYD+IK  +K A++  +KG
Sbjct: 211 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVVKAAADGPLKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE++VVS DF GD +SS FDA AGI L D FVKLV+WYDNE+GYS RV+DL+ HM+
Sbjct: 271 ILGYTEDQVVSCDFNGDSHSSTFDAGAGIALNDHFVKLVSWYDNEFGYSNRVVDLMVHMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
>sp|P46406|G3P_RABIT Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
          Length = 333

 Score =  356 bits (913), Expect = 5e-98
 Identities = 177/243 (72%), Positives = 201/243 (82%)
 Frame = +2

Query: 11  YVVESTGVFTTIEKASAHTKNNRAKKVVISAPSADAPMFVMGVNHEKYDPSLSIVSNASC 190
           YVVESTGVFTT+EKA AH K   AK+V+ISAPS DAPMFVMGVNHEKYD SL IVSNASC
Sbjct: 92  YVVESTGVFTTMEKAGAHLKGG-AKRVIISAPSXDAPMFVMGVNHEKYDNSLKIVSNASC 150

Query: 191 TTNCLAPLAKVVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXX 370
           TTNCLAPLAKV++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP    
Sbjct: 151 TTNCLAPLAKVIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTG 210

Query: 371 XXXXXXXXIPELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKG 550
                   IPELNGK+TGMAFRVP P+VSVVDLTC+L+KAAKYD+IK  +K ASE  +KG
Sbjct: 211 AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKG 270

Query: 551 ILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMS 730
           IL YTE++VVS DF    +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM+
Sbjct: 271 ILGYTEDQVVSCDFNSATHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVHMA 330

Query: 731 TKD 739
           +K+
Sbjct: 331 SKE 333
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,547,299
Number of Sequences: 369166
Number of extensions: 1709888
Number of successful extensions: 4822
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4353
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4453
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7454154400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)