Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_P19
(865 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CIF6|SIDT2_MOUSE SID1 transmembrane family member 2 pr... 79 2e-14
sp|Q8NBJ9|SIDT2_HUMAN SID1 transmembrane family member 2 pr... 79 2e-14
sp|Q9NXL6|SIDT1_HUMAN SID1 transmembrane family member 1 pr... 76 1e-13
sp|Q6AXF6|SIDT1_MOUSE SID1 transmembrane family member 1 pr... 75 2e-13
sp|Q6Q3F5|SIDT1_RAT SID1 transmembrane family member 1 prec... 75 2e-13
sp|Q9GZC8|SID1_CAEEL Systemic RNA interference defective pr... 45 3e-04
sp|P19145|GAP1_YEAST General amino-acid permease GAP1 36 0.16
sp|P51099|NU5C_ATRJA NAD(P)H-quinone oxidoreductase chain 5... 35 0.27
sp|Q49431|Y406_MYCGE Hypothetical protein MG406 35 0.27
sp|P51096|NU5C_ANIIN NAD(P)H-quinone oxidoreductase chain 5... 33 1.3
>sp|Q8CIF6|SIDT2_MOUSE SID1 transmembrane family member 2 precursor
Length = 832
Score = 79.0 bits (193), Expect = 2e-14
Identities = 33/68 (48%), Positives = 53/68 (77%)
Frame = +3
Query: 6 VFTISVFYGLPVIQLLWHFLMVMDENGDFDKCFFNDLCSKPLGSIISFNNVASNIGYIIL 185
+ TI+VFY LPV+QL+ + V++ G+ D C++N LC+ PLG++ +FNN+ SN+GYI+L
Sbjct: 453 IATIAVFYALPVVQLVITYQTVVNVTGNQDICYYNFLCAHPLGNLSAFNNILSNLGYILL 512
Query: 186 GITFILIV 209
G+ F+LI+
Sbjct: 513 GLLFLLII 520
Score = 63.2 bits (152), Expect = 9e-10
Identities = 26/49 (53%), Positives = 37/49 (75%), Gaps = 2/49 (4%)
Frame = +2
Query: 308 YGIFYALGFSLIMEGIMSASYHICPRSDTIQFDATFMYIIG--CLLNLY 448
+G+FYA+G +L+MEG++SA YH+CP QFD +FMY+I C+L LY
Sbjct: 547 FGLFYAMGTALMMEGLLSACYHVCPNYTNFQFDTSFMYMIAGLCMLKLY 595
>sp|Q8NBJ9|SIDT2_HUMAN SID1 transmembrane family member 2 precursor
Length = 832
Score = 79.0 bits (193), Expect = 2e-14
Identities = 33/68 (48%), Positives = 53/68 (77%)
Frame = +3
Query: 6 VFTISVFYGLPVIQLLWHFLMVMDENGDFDKCFFNDLCSKPLGSIISFNNVASNIGYIIL 185
+ TI+VFY LPV+QL+ + V++ G+ D C++N LC+ PLG++ +FNN+ SN+GYI+L
Sbjct: 453 IATIAVFYALPVVQLVITYQTVVNVTGNQDICYYNFLCAHPLGNLSAFNNILSNLGYILL 512
Query: 186 GITFILIV 209
G+ F+LI+
Sbjct: 513 GLLFLLII 520
Score = 63.2 bits (152), Expect = 9e-10
Identities = 26/49 (53%), Positives = 37/49 (75%), Gaps = 2/49 (4%)
Frame = +2
Query: 308 YGIFYALGFSLIMEGIMSASYHICPRSDTIQFDATFMYIIG--CLLNLY 448
+G+FYA+G +L+MEG++SA YH+CP QFD +FMY+I C+L LY
Sbjct: 547 FGLFYAMGTALMMEGLLSACYHVCPNYTNFQFDTSFMYMIAGLCMLKLY 595
>sp|Q9NXL6|SIDT1_HUMAN SID1 transmembrane family member 1 precursor
Length = 827
Score = 76.3 bits (186), Expect = 1e-13
Identities = 33/68 (48%), Positives = 51/68 (75%)
Frame = +3
Query: 6 VFTISVFYGLPVIQLLWHFLMVMDENGDFDKCFFNDLCSKPLGSIISFNNVASNIGYIIL 185
+ TI+VFY LPVIQL+ + V++ G+ D C++N LC+ PLG + +FNN+ SN+G+++L
Sbjct: 448 IITIAVFYALPVIQLVITYQTVVNVTGNQDICYYNFLCAHPLGVLSAFNNILSNLGHVLL 507
Query: 186 GITFILIV 209
G F+LIV
Sbjct: 508 GFLFLLIV 515
Score = 63.2 bits (152), Expect = 9e-10
Identities = 25/49 (51%), Positives = 37/49 (75%), Gaps = 2/49 (4%)
Frame = +2
Query: 308 YGIFYALGFSLIMEGIMSASYHICPRSDTIQFDATFMYIIG--CLLNLY 448
+G+FYA+G +L+MEG++SA YH+CP +FD +FMY+I C+L LY
Sbjct: 542 FGLFYAMGIALMMEGVLSACYHVCPNYSNFRFDTSFMYMIAGLCMLKLY 590
>sp|Q6AXF6|SIDT1_MOUSE SID1 transmembrane family member 1 precursor
Length = 827
Score = 75.1 bits (183), Expect = 2e-13
Identities = 32/68 (47%), Positives = 51/68 (75%)
Frame = +3
Query: 6 VFTISVFYGLPVIQLLWHFLMVMDENGDFDKCFFNDLCSKPLGSIISFNNVASNIGYIIL 185
+ TI+VFY LPV+QL+ + V++ G+ D C++N LC+ PLG + +FNN+ SN+G+++L
Sbjct: 448 IITIAVFYALPVMQLVITYQTVVNVTGNQDICYYNFLCAHPLGVLSAFNNILSNLGHVLL 507
Query: 186 GITFILIV 209
G F+LIV
Sbjct: 508 GFLFLLIV 515
Score = 64.7 bits (156), Expect = 3e-10
Identities = 26/49 (53%), Positives = 37/49 (75%), Gaps = 2/49 (4%)
Frame = +2
Query: 308 YGIFYALGFSLIMEGIMSASYHICPRSDTIQFDATFMYIIG--CLLNLY 448
+G+FYA+G +L+MEG++SA YH+CP QFD +FMY+I C+L LY
Sbjct: 542 FGLFYAMGIALMMEGVLSACYHVCPNYSNFQFDTSFMYMIAGLCMLKLY 590
>sp|Q6Q3F5|SIDT1_RAT SID1 transmembrane family member 1 precursor
Length = 831
Score = 75.1 bits (183), Expect = 2e-13
Identities = 32/68 (47%), Positives = 51/68 (75%)
Frame = +3
Query: 6 VFTISVFYGLPVIQLLWHFLMVMDENGDFDKCFFNDLCSKPLGSIISFNNVASNIGYIIL 185
+ TI+VFY LPV+QL+ + V++ G+ D C++N LC+ PLG + +FNN+ SN+G+++L
Sbjct: 447 IITIAVFYALPVMQLVITYQTVVNVTGNQDICYYNFLCAHPLGVLSAFNNILSNLGHVLL 506
Query: 186 GITFILIV 209
G F+LIV
Sbjct: 507 GFLFLLIV 514
Score = 64.7 bits (156), Expect = 3e-10
Identities = 26/49 (53%), Positives = 37/49 (75%), Gaps = 2/49 (4%)
Frame = +2
Query: 308 YGIFYALGFSLIMEGIMSASYHICPRSDTIQFDATFMYIIG--CLLNLY 448
+G+FYA+G +L+MEG++SA YH+CP QFD +FMY+I C+L LY
Sbjct: 541 FGLFYAMGIALMMEGVLSACYHVCPNYSNFQFDTSFMYMIAGLCMLKLY 589
>sp|Q9GZC8|SID1_CAEEL Systemic RNA interference defective protein 1 precursor (Systemic
RNAi enabling protein)
Length = 776
Score = 44.7 bits (104), Expect = 3e-04
Identities = 16/38 (42%), Positives = 27/38 (71%)
Frame = +3
Query: 93 DKCFFNDLCSKPLGSIISFNNVASNIGYIILGITFILI 206
+ CF N C++PLG + ++NN+ +NIGY + G FI++
Sbjct: 462 EMCFHNHACARPLGELRAWNNIITNIGYTLYGAIFIVL 499
Score = 36.2 bits (82), Expect = 0.12
Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 8/84 (9%)
Frame = +2
Query: 212 NQLFCKRGKTLLSRMF*KII*KLIKNSQN--SRLYGIF------YALGFSLIMEGIMSAS 367
N + G TL +F I+ + + ++ S ++G + +G ++++ I SA+
Sbjct: 481 NNIITNIGYTLYGAIF--IVLSICRRGRHEYSHVFGTYECTLLDVTIGVFMVLQSIASAT 538
Query: 368 YHICPRSDTIQFDATFMYIIGCLL 439
YHICP QFD + +I LL
Sbjct: 539 YHICPSDVAFQFDTPCIQVICGLL 562
>sp|P19145|GAP1_YEAST General amino-acid permease GAP1
Length = 602
Score = 35.8 bits (81), Expect = 0.16
Identities = 27/81 (33%), Positives = 42/81 (51%), Gaps = 2/81 (2%)
Frame = +2
Query: 587 YKENINIFIKWLVILIFQSPIISISLLNIFKSSALKWSDFFICSRFVIYANIIIF-VAFY 763
Y N N ++WLV+L + I+S S+ F + K+ D F+ ++ I +F V Y
Sbjct: 169 YANNFNYMLQWLVVLPLE--IVSASITVNFWGTDPKYRDGFVALFWLAIVIINMFGVKGY 226
Query: 764 LIKEFYFYFL-VAIVVNFVVL 823
EF F F+ V VV F++L
Sbjct: 227 GEAEFVFSFIKVITVVGFIIL 247
>sp|P51099|NU5C_ATRJA NAD(P)H-quinone oxidoreductase chain 5, chloroplast (NAD(P)H
dehydrogenase, chain 5) (NADH-plastoquinone
oxidoreductase chain 5)
Length = 749
Score = 35.0 bits (79), Expect = 0.27
Identities = 25/76 (32%), Positives = 40/76 (52%), Gaps = 1/76 (1%)
Frame = +2
Query: 596 NINIFIKWLVILIFQSPIISISLLNIFKSSALKWSDFFICSRF-VIYANIIIFVAFYLIK 772
N++I KWL SI+LL+ ++++ W +FF + F V A+ IF+AF+L K
Sbjct: 575 NLDILSKWLTP--------SINLLHKNSNNSIDWYEFFKDAVFSVSIASFGIFIAFFLYK 626
Query: 773 EFYFYFLVAIVVNFVV 820
Y F ++N V
Sbjct: 627 PVYSSFQNLDLINSFV 642
>sp|Q49431|Y406_MYCGE Hypothetical protein MG406
Length = 113
Score = 35.0 bits (79), Expect = 0.27
Identities = 21/71 (29%), Positives = 39/71 (54%), Gaps = 10/71 (14%)
Frame = +2
Query: 659 SLLNIFKSSALKWSDFFICSRFVIYANIIIFVAFYLIKEFY----------FYFLVAIVV 808
SL N+ K A+ + FF +R++IY I + ++F + + F+ ++ +V+
Sbjct: 23 SLKNVKKQKAVSFFAFFFTARYLIYL-IPVIISFVVTPSIFNTIATIISTLFFPILNLVL 81
Query: 809 NFVVLLLEYYF 841
+FV L LEY+F
Sbjct: 82 SFVWLPLEYFF 92
>sp|P51096|NU5C_ANIIN NAD(P)H-quinone oxidoreductase chain 5, chloroplast (NAD(P)H
dehydrogenase, chain 5) (NADH-plastoquinone
oxidoreductase chain 5)
Length = 741
Score = 32.7 bits (73), Expect = 1.3
Identities = 23/79 (29%), Positives = 41/79 (51%), Gaps = 1/79 (1%)
Frame = +2
Query: 587 YKENINIFIKWLVILIFQSPIISISLLNIFKSSALKWSDFFICSRF-VIYANIIIFVAFY 763
+ ++++I KWL SI+LL+ ++++ W +F + F V A+ IF+AF+
Sbjct: 569 FNQDVDILTKWLTP--------SINLLHKNSNNSIDWYEFSKDAVFSVSIASFGIFIAFF 620
Query: 764 LIKEFYFYFLVAIVVNFVV 820
L K Y F ++N V
Sbjct: 621 LYKPVYSSFQNLDLINSFV 639
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,508,324
Number of Sequences: 369166
Number of extensions: 1747218
Number of successful extensions: 4534
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4349
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4531
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)