Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_O13
(813 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RIN... 59 2e-08
sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6 57 5e-08
sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5 57 6e-08
sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Int... 56 1e-07
sp|P68907|PZRN3_RAT PDZ domain-containing RING finger prote... 54 5e-07
sp|Q69ZS0|PZRN3_MOUSE PDZ domain-containing RING finger pro... 52 2e-06
sp|Q9UPQ7|PZRN3_HUMAN PDZ domain-containing RING finger pro... 49 1e-05
sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD4... 47 6e-05
sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD4... 47 6e-05
sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cys... 46 1e-04
>sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RING finger protein 84)
Length = 557
Score = 58.5 bits (140), Expect = 2e-08
Identities = 38/140 (27%), Positives = 67/140 (47%), Gaps = 6/140 (4%)
Frame = +2
Query: 221 SACSGNTEECKLENISQTVINHRLNEDISNLTVKCPEEK--CSFECRLI----DMDEHLK 382
++C E+C VIN + +E+ NL CPE C C I ++DEHL
Sbjct: 159 ASCQFRKEKCLYCKKDVVVINLQNHEE--NL---CPEYPVFCPNNCAKIILKTEVDEHLA 213
Query: 383 VCDNKTEKCPFFGYGCTHKIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREKEKN 562
VC + CPF YGC K +N+ H ++H ++++ L+ ++ DL + +
Sbjct: 214 VCPEAEQDCPFKHYGCAVTDKRRNLQQHEHSALREHMRLVLEKNVQLEEQISDLHKSLEQ 273
Query: 563 NHDEIEKLKEKGRVQDEKLK 622
+I++L E + +++ K
Sbjct: 274 KESKIQQLAETIKKLEKEFK 293
Score = 40.0 bits (92), Expect = 0.008
Identities = 34/162 (20%), Positives = 65/162 (40%), Gaps = 2/162 (1%)
Frame = +2
Query: 83 KFSVIGEITNDMKCTLCGNIAVNAQQALCGCNFCNKCITNFIN-NGGSACSGNTEECKLE 259
++ + + KC C ++ N Q CG FC CI + N C + E K +
Sbjct: 32 EYQFVERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCILSLRELNTVPICPVDKEVIKSQ 91
Query: 260 NISQTVINHRLNEDISNLTVKCPEEK-CSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTH 436
+ + ++ ++ NL V C C+ + L +HL+ C + +C C
Sbjct: 92 EVFK---DNCCKREVLNLYVYCSNAPGCNAKVILGRYQDHLQQCLFQPVQCS--NEKCRE 146
Query: 437 KIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREKEKN 562
+ K++ H+ Q + ++ VI+L+ E+N
Sbjct: 147 PVLRKDLKEHLSASCQFRKEKCLYCKKDV--VVINLQNHEEN 186
>sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6
Length = 530
Score = 57.4 bits (137), Expect = 5e-08
Identities = 39/152 (25%), Positives = 64/152 (42%)
Frame = +2
Query: 119 KCTLCGNIAVNAQQALCGCNFCNKCITNFINNGGSACSGNTEECKLENISQTVINHRLNE 298
+C +C A Q CG FC CI I + G C + E LEN Q ++
Sbjct: 69 ECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD-NEILLEN--QLFPDNFAKR 125
Query: 299 DISNLTVKCPEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLRE 478
+I +LTVKCP + C + L +++H C+ CP C + +N+H++ +
Sbjct: 126 EILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCP----QCQRPFQKCQVNTHIIED 181
Query: 479 AQDHNNMLIDLLGNLKNEVIDLREKEKNNHDE 574
+ N + + +EK HD+
Sbjct: 182 CPRRQVSCV-------NCAVSMAYEEKEIHDQ 206
>sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5
Length = 558
Score = 57.0 bits (136), Expect = 6e-08
Identities = 35/125 (28%), Positives = 59/125 (47%)
Frame = +2
Query: 248 CKLENISQTVINHRLNEDISNLTVKCPEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYG 427
CK + + + +H N V CP +C ++EHL VC + CPF YG
Sbjct: 171 CKRDIVVTNLQDHEENS-CPAYPVSCPN-RCVQTIPRARVNEHLTVCPEAEQDCPFKHYG 228
Query: 428 CTHKIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREKEKNNHDEIEKLKEKGRVQ 607
CT K K N+ H QDH ++++ L+ + DL + + +I++L E +
Sbjct: 229 CTVKGKRGNLLEHERAALQDHMLLVLEKNYQLEQRISDLYQSLEQKESKIQQLAETVKKF 288
Query: 608 DEKLK 622
+++LK
Sbjct: 289 EKELK 293
Score = 45.8 bits (107), Expect = 1e-04
Identities = 30/133 (22%), Positives = 55/133 (41%), Gaps = 2/133 (1%)
Frame = +2
Query: 77 DHKFSVIGEITNDMKCTLCGNIAVNAQQALCGCNFCNKCITNFIN-NGGSACSGNTEECK 253
D ++ + ++ KC C ++ N Q CG FC +CI + N C + E K
Sbjct: 30 DTEYQFVEQLEERYKCAFCHSVLHNPHQTGCGHRFCQQCIRSLRELNSVPICPVDKEVIK 89
Query: 254 LENISQTVINHRLNEDISNLTVKCPEEK-CSFECRLIDMDEHLKVCDNKTEKCPFFGYGC 430
+ + + ++ ++ NL V C C+ L +HL+ C + CP C
Sbjct: 90 PQEVFK---DNCCKREVLNLHVYCKNAPGCNARIILGRFQDHLQHCSFQAVPCP--NESC 144
Query: 431 THKIKSKNMNSHM 469
+ K++ H+
Sbjct: 145 REAMLRKDVKEHL 157
>sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Interleukin 1 signal transducer)
(RING finger protein 85)
Length = 522
Score = 56.2 bits (134), Expect = 1e-07
Identities = 35/120 (29%), Positives = 54/120 (45%)
Frame = +2
Query: 119 KCTLCGNIAVNAQQALCGCNFCNKCITNFINNGGSACSGNTEECKLENISQTVINHRLNE 298
+C +C A Q CG FC CI I + G C + E LEN Q ++
Sbjct: 69 ECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVD-NEILLEN--QLFPDNFAKR 125
Query: 299 DISNLTVKCPEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTHKIKSKNMNSHMLRE 478
+I +L VKCP E C + L +++H C+ CP C + ++N H+L++
Sbjct: 126 EILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP----QCQRPFQKFHINIHILKD 181
>sp|P68907|PZRN3_RAT PDZ domain-containing RING finger protein 3 (Semaphorin cytoplasmic
domain-associated protein 3) (SEMACAP3 protein)
Length = 1062
Score = 53.9 bits (128), Expect = 5e-07
Identities = 40/134 (29%), Positives = 61/134 (45%), Gaps = 4/134 (2%)
Frame = +2
Query: 98 GEITNDMKCTLCGNIAVNAQQALCGCNFCNKCITNFINNGGSACSGNTEECKLENISQTV 277
GE+ D+KC LC + + CG FC C+ ++ GS S C+ +S
Sbjct: 10 GEVDPDLKCALCHKVLEDPLTTPCGHVFCAGCVLPWVVQEGSCPS----RCR-GRLSAKE 64
Query: 278 INH--RLNEDISNLTVKCPE--EKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTHKIK 445
+NH L I L +KC C +L D+ EHL+ CD +C GC +
Sbjct: 65 LNHVLPLKRLILKLDIKCAHAARGCGRVVKLQDLPEHLERCDFAPARCRH--AGCGQLLL 122
Query: 446 SKNMNSHMLREAQD 487
+++ +HM R+A D
Sbjct: 123 RRDVEAHM-RDACD 135
>sp|Q69ZS0|PZRN3_MOUSE PDZ domain-containing RING finger protein 3 (Semaphorin cytoplasmic
domain-associated protein 3) (SEMACAP3 protein)
Length = 1063
Score = 52.0 bits (123), Expect = 2e-06
Identities = 39/137 (28%), Positives = 60/137 (43%), Gaps = 7/137 (5%)
Frame = +2
Query: 98 GEITNDMKCTLCGNIAVNAQQALCGCNFCNKCITNFINNGGSA---CSGNTEECKLENIS 268
G++ D+KC LC + + CG FC C+ ++ GS C G +S
Sbjct: 10 GDVDPDLKCALCHKVLEDPLTTPCGHVFCAGCVLPWVVQEGSCPARCRG--------RLS 61
Query: 269 QTVINH--RLNEDISNLTVKCPE--EKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTH 436
+NH L I L +KC C +L D+ EHL+ CD +C GC
Sbjct: 62 AKELNHVLPLKRLILKLDIKCAHAARGCGRVVKLQDLPEHLERCDFAPARCRH--AGCGQ 119
Query: 437 KIKSKNMNSHMLREAQD 487
+ +++ +HM R+A D
Sbjct: 120 LLLRRDVEAHM-RDACD 135
>sp|Q9UPQ7|PZRN3_HUMAN PDZ domain-containing RING finger protein 3 (Ligand of Numb-protein
X 3) (Semaphorin cytoplasmic domain-associated protein
3) (SEMACAP3 protein)
Length = 1066
Score = 49.3 bits (116), Expect = 1e-05
Identities = 38/137 (27%), Positives = 59/137 (43%), Gaps = 7/137 (5%)
Frame = +2
Query: 98 GEITNDMKCTLCGNIAVNAQQALCGCNFCNKCITNFINNGGSA---CSGNTEECKLENIS 268
G++ D+KC LC + + CG FC C+ ++ GS C G +S
Sbjct: 10 GDVDPDLKCALCHKVLEDPLTTPCGHVFCAGCVLPWVVQEGSCPARCRG--------RLS 61
Query: 269 QTVINH--RLNEDISNLTVKC--PEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCTH 436
+NH L I L +KC C +L + EHL+ CD +C GC
Sbjct: 62 AKELNHVLPLKRLILKLDIKCAYATRGCGRVVKLQQLPEHLERCDFAPARCRH--AGCGQ 119
Query: 437 KIKSKNMNSHMLREAQD 487
+ +++ +HM R+A D
Sbjct: 120 VLLRRDVEAHM-RDACD 135
>sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (CD40 binding protein) (CD40BP) (LMP1
associated protein) (LAP1) (CAP-1)
Length = 568
Score = 47.0 bits (110), Expect = 6e-05
Identities = 33/134 (24%), Positives = 59/134 (44%), Gaps = 4/134 (2%)
Frame = +2
Query: 248 CKLENISQTVINHRLNEDISNLTVKCPEEKCSFECRL-IDMDEHLKVCDNKTEKCPFFGY 424
CK + + + + D + V CP KCS + L ++ HL C N C F Y
Sbjct: 180 CKSQ-VPMIALQKHEDTDCPCVVVSCPH-KCSVQTLLRSELSAHLSECVNAPSTCSFKRY 237
Query: 425 GCTHKIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREKEKNNHDEIEKLKEK--- 595
GC + ++ + +H A H N+L + +L+ +V L+ + + I+ L +
Sbjct: 238 GCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICS 297
Query: 596 GRVQDEKLKAMYEN 637
++ E+ K M N
Sbjct: 298 FEIEIERQKEMLRN 311
Score = 41.6 bits (96), Expect = 0.003
Identities = 28/132 (21%), Positives = 55/132 (41%), Gaps = 2/132 (1%)
Frame = +2
Query: 80 HKFSVIGEITNDMKCTLCGNIAVNAQQALCGCNFCNKCITNFINNGGSACSGNTEECKLE 259
+K + + + KC C + + +Q CG FC C+ +++ C+ E +
Sbjct: 39 YKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKD 98
Query: 260 NISQTVINHRLNEDISNLTVKCPEEK--CSFECRLIDMDEHLKVCDNKTEKCPFFGYGCT 433
+ + ++ +I L + C E C+ + L + HLK D E+ P C
Sbjct: 99 KVFK---DNCCKREILALQIYCRNESRGCAEQLTLGHLLVHLK-NDCHFEELPCVRPDCK 154
Query: 434 HKIKSKNMNSHM 469
K+ K++ H+
Sbjct: 155 EKVLRKDLRDHV 166
>sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (TRAFAMN)
Length = 567
Score = 47.0 bits (110), Expect = 6e-05
Identities = 36/143 (25%), Positives = 59/143 (41%), Gaps = 4/143 (2%)
Frame = +2
Query: 221 SACSGNTEECKLENISQTVINHRLNEDISNLTVKCPEEKCSFECRL-IDMDEHLKVCDNK 397
S C KL+ T D + V CP KCS + L ++ HL C N
Sbjct: 177 SHCKSQVPMIKLQKHEDT--------DCPCVVVSCPH-KCSVQTLLRSELSAHLSECVNA 227
Query: 398 TEKCPFFGYGCTHKIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREKEKNNHDEI 577
C F YGC + ++ + +H A H N+L + +L+ +V L+ + + I
Sbjct: 228 PSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSI 287
Query: 578 EKLKEK---GRVQDEKLKAMYEN 637
+ L + ++ E+ K M N
Sbjct: 288 QSLHNQICSFEIEIERQKEMLRN 310
Score = 42.7 bits (99), Expect = 0.001
Identities = 29/132 (21%), Positives = 56/132 (42%), Gaps = 2/132 (1%)
Frame = +2
Query: 80 HKFSVIGEITNDMKCTLCGNIAVNAQQALCGCNFCNKCITNFINNGGSACSGNTEECKLE 259
+K + + + KC C + N +Q CG FC C+ +++ C+ E +
Sbjct: 38 YKEKFVKTVEDKYKCEKCRLVLCNPKQTECGHRFCESCMAALLSSSSPKCTACQESIIKD 97
Query: 260 NISQTVINHRLNEDISNLTVKCPEE--KCSFECRLIDMDEHLKVCDNKTEKCPFFGYGCT 433
+ + ++ +I L V C E C+ + L + HLK + + E+ P C
Sbjct: 98 KVFK---DNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLK-NECQFEELPCLRADCK 153
Query: 434 HKIKSKNMNSHM 469
K+ K++ H+
Sbjct: 154 EKVLRKDLRDHV 165
>sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cysteine-rich domain associated
with RING and Traf domains protein 1) (Malignant 62)
(RING finger protein 83)
Length = 470
Score = 45.8 bits (107), Expect = 1e-04
Identities = 40/162 (24%), Positives = 64/162 (39%), Gaps = 16/162 (9%)
Frame = +2
Query: 116 MKCTLCG----NIAVNAQQALCGCN--FC-NKCITNFIN-----NGGSACSGNTEECKL- 256
+KC CG A + + +C +C NKC + + S C T+ C
Sbjct: 139 LKCEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYC 198
Query: 257 --ENISQTVINHRLNEDISNLTVKCPEEKCSFECRLIDMDEHLK-VCDNKTEKCPFFGYG 427
E + T+ +H+ L V CP + D+ HLK C+ CPF G
Sbjct: 199 TKEFVFDTIQSHQYQ--CPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSG 256
Query: 428 CTHKIKSKNMNSHMLREAQDHNNMLIDLLGNLKNEVIDLREK 553
C H+ M H+ + H M+ L+ + E+ +LR +
Sbjct: 257 CKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRE 298
Score = 44.3 bits (103), Expect = 4e-04
Identities = 32/134 (23%), Positives = 58/134 (43%), Gaps = 3/134 (2%)
Frame = +2
Query: 77 DHKFSVIGEITNDMKCTLCGN-IAVNAQQALCGCNFCNKCITNFINNGGSACSGNTEECK 253
D+KF + + + C LCG + Q + CG FC+ C+ F++ G C E+
Sbjct: 5 DYKF--LEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKC---PEDQL 59
Query: 254 LENISQTVINHRLNEDISNLTVKC--PEEKCSFECRLIDMDEHLKVCDNKTEKCPFFGYG 427
+ ++ + L + L ++C EE C + L + HL C CP
Sbjct: 60 PLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCP---NR 116
Query: 428 CTHKIKSKNMNSHM 469
C K+ +++ +H+
Sbjct: 117 CPMKLSRRDLPAHL 130
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,137,944
Number of Sequences: 369166
Number of extensions: 1583010
Number of successful extensions: 5734
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5272
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5690
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7763237265
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)