Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_N02
(548 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9BYM8|UB7I3_HUMAN Ubiquitin-conjugating enzyme 7-intera... 60 4e-09
sp|Q62921|UB7I3_RAT Ubiquitin-conjugating enzyme 7-interact... 58 2e-08
sp|Q9WUB0|UB7I3_MOUSE Ubiquitin-conjugating enzyme 7-intera... 57 2e-08
sp|Q924T7|RNF31_MOUSE RING finger protein 31 51 2e-06
sp|Q96EP0|RNF31_HUMAN RING finger protein 31 (Zinc in-betwe... 50 5e-06
sp|Q9NWF9|UB7I1_HUMAN E3 ubiquitin ligase TRIAD3 (Ubiquitin... 36 0.054
sp|P58283|UB7I1_MOUSE E3 ubiquitin ligase TRIAD3 (Ubiquitin... 36 0.054
sp|P26372|KRUC_SHEEP Keratin, ultra high-sulfur matrix prot... 35 0.16
sp|Q8BKD6|IBRD2_MOUSE E3 ubiquitin ligase IBRDC2 (IBR domai... 33 0.35
sp|Q6L8H1|KRA54_HUMAN Keratin-associated protein 5-4 (Kerat... 33 0.46
>sp|Q9BYM8|UB7I3_HUMAN Ubiquitin-conjugating enzyme 7-interacting protein 3 (Hepatitis B
virus X-associated protein 4) (HBV-associated factor 4)
(RING finger protein 54)
Length = 500
Score = 60.1 bits (144), Expect = 4e-09
Identities = 40/119 (33%), Positives = 58/119 (48%), Gaps = 16/119 (13%)
Frame = +2
Query: 5 FICPVCKHSNCLDCEDVHEDKSCEEFR-----LTERYIDAQKA----KKKLQGESGIKCI 157
F CPVC H NCL C+ +HE +C+E++ + + A++ K LQ ++C
Sbjct: 379 FTCPVCFHVNCLLCKAIHEQMNCKEYQEDLALRAQNDVAARQTTEMLKVMLQQGEAMRCP 438
Query: 158 CGRFLVQ-KGGCGALHCDSCGRQICVFTKKIA-GNNGP-DTCGCTPTKT----CHPDCK 313
+ +VQ K GC + C C +IC TK G GP DT G + CHP C+
Sbjct: 439 QCQIVVQKKDGCDWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQ 497
>sp|Q62921|UB7I3_RAT Ubiquitin-conjugating enzyme 7-interacting protein 3 (RBCC protein
interacting with PKC) (Protein kinase C-binding protein
beta-15)
Length = 498
Score = 57.8 bits (138), Expect = 2e-08
Identities = 41/119 (34%), Positives = 56/119 (47%), Gaps = 16/119 (13%)
Frame = +2
Query: 5 FICPVCKHSNCLDCEDVHEDKSCEEFR--LTERY---IDAQKAKKKL-----QGESGIKC 154
F CPVC NCL C+ +HE +C E++ L R + AQ+ + L QGE+
Sbjct: 377 FTCPVCTRVNCLLCKAIHERMNCREYQDDLAHRARNDVAAQQTTEMLRVMLQQGEAMYCP 436
Query: 155 ICGRFLVQKGGCGALHCDSCGRQICVFTKKIA-GNNGP-DTCGCTPTKT----CHPDCK 313
C + +K GC + C C +IC TK G GP DT G + CHP C+
Sbjct: 437 QCRIVVQKKDGCDWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQ 495
>sp|Q9WUB0|UB7I3_MOUSE Ubiquitin-conjugating enzyme 7-interacting protein 3
(UbcM4-interacting protein 28)
Length = 498
Score = 57.4 bits (137), Expect = 2e-08
Identities = 40/119 (33%), Positives = 55/119 (46%), Gaps = 16/119 (13%)
Frame = +2
Query: 5 FICPVCKHSNCLDCEDVHEDKSCEEFR-----LTERYIDAQKA----KKKLQGESGIKCI 157
F CPVC NCL C+ +HE +C E++ + + A++ K LQ + C
Sbjct: 377 FTCPVCTRVNCLLCKAIHEHMNCREYQDDLALRAQNDVAARQTTEMLKVMLQQGEAMHCP 436
Query: 158 CGRFLVQ-KGGCGALHCDSCGRQICVFTKKIA-GNNGP-DTCGCTPTKT----CHPDCK 313
R +VQ K GC + C C +IC TK G GP DT G + CHP C+
Sbjct: 437 QCRIVVQKKDGCDWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQ 495
>sp|Q924T7|RNF31_MOUSE RING finger protein 31
Length = 1066
Score = 50.8 bits (120), Expect = 2e-06
Identities = 37/109 (33%), Positives = 50/109 (45%), Gaps = 12/109 (11%)
Frame = +2
Query: 11 CPVCKHSNCLDC----EDVHEDKSCEEFRLTERYID----AQKAKKKLQGESGIKCICGR 166
CP C + C+ C E+ H +SCE+F+ +R D AQ LQ E+GI C +
Sbjct: 811 CPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRTNDPEYQAQGLAMYLQ-ENGIDCPKCK 869
Query: 167 F--LVQKGGCGALHCDSCGRQIC--VFTKKIAGNNGPDTCGCTPTKTCH 301
F + +GGC HC C Q C + A N PD C K+ H
Sbjct: 870 FSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDP-NCKVKKSLH 917
>sp|Q96EP0|RNF31_HUMAN RING finger protein 31 (Zinc in-between-RING-finger
ubiquitin-associated domain protein)
Length = 1072
Score = 49.7 bits (117), Expect = 5e-06
Identities = 36/109 (33%), Positives = 50/109 (45%), Gaps = 12/109 (11%)
Frame = +2
Query: 11 CPVCKHSNCLDC----EDVHEDKSCEEFRLTERYID----AQKAKKKLQGESGIKCICGR 166
CP C + C+ C E+ H +SCE+F+ +R D AQ LQ E+GI C +
Sbjct: 817 CPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQ-ENGIDCPKCK 875
Query: 167 F--LVQKGGCGALHCDSCGRQIC--VFTKKIAGNNGPDTCGCTPTKTCH 301
F + +GGC HC C Q C + A N P+ C K+ H
Sbjct: 876 FSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEP-NCRVKKSLH 923
>sp|Q9NWF9|UB7I1_HUMAN E3 ubiquitin ligase TRIAD3 (Ubiquitin conjugating enzyme 7
interacting protein 1) (Zinc finger protein inhibiting
NF-kappa-B)
Length = 866
Score = 36.2 bits (82), Expect = 0.054
Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 4/76 (5%)
Frame = +2
Query: 14 PVCKHSNCLDCEDV---HEDKSCEEFRLTERYIDAQKAKKKLQGESGIKCI-CGRFLVQK 181
P C+ C C+ + H +CEE + ++K+ KC CG L++
Sbjct: 626 PHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKS 685
Query: 182 GGCGALHCDSCGRQIC 229
GC + C CG Q+C
Sbjct: 686 EGCNRMSC-RCGAQMC 700
>sp|P58283|UB7I1_MOUSE E3 ubiquitin ligase TRIAD3 (Ubiquitin-conjugating enzyme
7-interacting protein 1) (UbcM4-interacting protein 83)
Length = 853
Score = 36.2 bits (82), Expect = 0.054
Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 4/76 (5%)
Frame = +2
Query: 14 PVCKHSNCLDCEDV---HEDKSCEEFRLTERYIDAQKAKKKLQGESGIKCI-CGRFLVQK 181
P C+ C C+ + H +CEE + ++K+ KC CG L++
Sbjct: 614 PRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKS 673
Query: 182 GGCGALHCDSCGRQIC 229
GC + C CG Q+C
Sbjct: 674 EGCNRMSC-RCGAQMC 688
>sp|P26372|KRUC_SHEEP Keratin, ultra high-sulfur matrix protein (UHS keratin)
Length = 182
Score = 34.7 bits (78), Expect = 0.16
Identities = 23/66 (34%), Positives = 28/66 (42%), Gaps = 10/66 (15%)
Frame = +2
Query: 143 GIKCICGRFLVQKGGCGALH-CDS-CGRQICV--------FTKKIAGNNGPDTCGCTPTK 292
G K CG KGGCG+ C S CG CV + G G +CGC+ +
Sbjct: 112 GSKGGCGSCGGSKGGCGSCGGCGSGCGSSCCVPVCCCVPACSCSSCGKGGCGSCGCSQSS 171
Query: 293 TCHPDC 310
C P C
Sbjct: 172 CCVPVC 177
>sp|Q8BKD6|IBRD2_MOUSE E3 ubiquitin ligase IBRDC2 (IBR domain-containing protein 2)
Length = 301
Score = 33.5 bits (75), Expect = 0.35
Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Frame = +2
Query: 11 CPVCKHSNCLDCEDV-HEDKSCEEFRLTERYIDAQKAKKKLQGESGIK-C-ICGRFLVQK 181
CP C C C+D HE+ SC + ++ + A ++ IK C +C ++ +
Sbjct: 145 CPSCHLKFCSCCKDAWHEESSCRD---SQSAMPEHGALFGTDADAPIKQCPVCRIYIERN 201
Query: 182 GGCGALHCDSCGRQIC 229
GC + C +C C
Sbjct: 202 EGCAQMMCKNCKHTFC 217
>sp|Q6L8H1|KRA54_HUMAN Keratin-associated protein 5-4 (Keratin-associated protein 5.4)
(Ultrahigh sulfur keratin-associated protein 5.4)
Length = 288
Score = 33.1 bits (74), Expect = 0.46
Identities = 22/66 (33%), Positives = 25/66 (37%), Gaps = 6/66 (9%)
Frame = +2
Query: 143 GIKCICGRFLVQKGGCGAL-----HCDSCGRQICVFTKKIAGNNGPDTCGCTPTKTCHP- 304
G K CG KGGCG+ C SCG G +CGC+ C P
Sbjct: 156 GSKGGCGSCGGSKGGCGSCGGSKGGCGSCG----------GSKGGCGSCGCSQCSCCKPC 205
Query: 305 DCKYGC 322
C GC
Sbjct: 206 CCSSGC 211
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,933,536
Number of Sequences: 369166
Number of extensions: 1037275
Number of successful extensions: 2651
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2491
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2618
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3833118120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)