Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_M08
(508 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP... 150 2e-36
sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP... 139 3e-33
sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mi... 139 5e-33
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 135 4e-32
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 130 2e-30
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 123 2e-28
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 120 2e-27
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 114 1e-25
sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cy... 114 1e-25
sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cy... 113 2e-25
>sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 619
Score = 150 bits (379), Expect = 2e-36
Identities = 69/124 (55%), Positives = 92/124 (74%), Gaps = 2/124 (1%)
Frame = +1
Query: 1 WLDLEKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGE-DIAEPSPIG 177
W+DL K GRK+P I+HVNWFR + KF+WPG+G+NIRVIDW+ RR++GE DI +PIG
Sbjct: 493 WIDLNKDGRKVPKIYHVNWFRRDANNKFLWPGYGQNIRVIDWIVRRLDGEPDIGVDTPIG 552
Query: 178 LLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
++PK G IN +GL D +WDE+ S+PK Y + D +E KFL EQ G DLP I E++ QE
Sbjct: 553 IVPKKGAINASGLPDIQWDELMSVPKEYWTNDAKEIRKFLEEQVGPDLPKEIRAEMDAQE 612
Query: 355 KRIH 366
+RI+
Sbjct: 613 ERIN 616
>sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 643
Score = 139 bits (351), Expect = 3e-33
Identities = 66/123 (53%), Positives = 86/123 (69%), Gaps = 2/123 (1%)
Frame = +1
Query: 1 WLDLEKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGE-DIAEPSPIG 177
W+ L +P K+P IFHVNWFR + KF+WPG+G+NIRVIDW+ RR +G+ IAE +PIG
Sbjct: 516 WMKLGQPPHKVPKIFHVNWFRQSADHKFLWPGYGDNIRVIDWILRRCSGDATIAEETPIG 575
Query: 178 LLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
+PK GTIN+ GL + WDE+ S+PK+Y ED+ ET F Q G DLP I +EL Q
Sbjct: 576 FIPKKGTINLEGLPNVNWDELMSIPKSYWLEDMVETKTFFENQVGSDLPPEIAKELEAQT 635
Query: 355 KRI 363
+RI
Sbjct: 636 ERI 638
>sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 139 bits (349), Expect = 5e-33
Identities = 61/126 (48%), Positives = 90/126 (71%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDLE-KPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIG 177
WL +E + G ++P IFHVNWFR ++ G F+WPGFGEN RV+DW+CRR+ GED A +PIG
Sbjct: 515 WLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIG 574
Query: 178 LLPKHGTINVTGLDA-KWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
L+PK G ++++GL A ++FSLPK + ++V++ +L EQ +DLP ++ EL E
Sbjct: 575 LVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALE 634
Query: 355 KRIHNM 372
+R+H M
Sbjct: 635 RRVHKM 640
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 135 bits (341), Expect = 4e-32
Identities = 59/126 (46%), Positives = 90/126 (71%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDLE-KPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIG 177
WL +E + G ++P IFHVNWFR ++ G+F+WPGFGEN RV+DW+CRR+ GED A+ +PIG
Sbjct: 515 WLSMEGQKGARLPRIFHVNWFRRDEAGRFLWPGFGENARVLDWICRRLEGEDSAQETPIG 574
Query: 178 LLPKHGTINVTGLDA-KWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
L+PK G ++++GL A ++FS+PK + ++V++ +L EQ +DLP ++ EL E
Sbjct: 575 LVPKEGALDLSGLSAVDTSQLFSIPKDFWEQEVRDIRGYLTEQVNQDLPKEVLAELEALE 634
Query: 355 KRIHNM 372
R+ M
Sbjct: 635 GRVQKM 640
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 130 bits (326), Expect = 2e-30
Identities = 61/126 (48%), Positives = 86/126 (68%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDLEKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGL 180
WL +EK G+ +P IFHVNWFR + +GKF+WPG+GEN RV++W+ RRVNGE S IG
Sbjct: 523 WLSMEKRGQ-VPKIFHVNWFRKSAEGKFMWPGYGENSRVLEWILRRVNGESCYVDSAIGH 581
Query: 181 LPKHGTINVTGLDAKWD--EMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
+P G +N+ G+ K D E+FSLPK + S++V++ + Q G DLPA I Q+L+E
Sbjct: 582 IPAEGALNLDGMKDKVDVKEIFSLPKEFWSQEVKDIRTYFESQVGADLPASIYQQLDELS 641
Query: 355 KRIHNM 372
R+ N+
Sbjct: 642 SRVDNL 647
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 123 bits (309), Expect = 2e-28
Identities = 58/126 (46%), Positives = 83/126 (65%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIG 177
WL + +P K+P IFHVNWFR + GKF+WPGFGEN RV++WM R+ GED A+ +PIG
Sbjct: 497 WLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIG 556
Query: 178 LLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
+PK +N+ GL D +E+F + K + ++V+E K+L +Q DLP I +EL +
Sbjct: 557 YVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALK 616
Query: 355 KRIHNM 372
+RI M
Sbjct: 617 QRISQM 622
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 120 bits (300), Expect = 2e-27
Identities = 56/126 (44%), Positives = 82/126 (65%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIG 177
WL + +P K+P IFHVNWFR + GKF+WPGFGEN RV++WM R+ GED A+ +PIG
Sbjct: 497 WLSMAHRPAAKLPKIFHVNWFRKDKDGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIG 556
Query: 178 LLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
+PK +N+ GL +E+F + K + ++V+E ++L +Q DLP I +EL +
Sbjct: 557 YIPKENALNLKGLGGVNVEELFGISKEFWEKEVEEIDRYLEDQVNTDLPYEIERELRALK 616
Query: 355 KRIHNM 372
+RI M
Sbjct: 617 QRISQM 622
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 114 bits (285), Expect = 1e-25
Identities = 53/126 (42%), Positives = 82/126 (65%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIG 177
WL + + P K+P IFHVNWFR + +GKF+WPGFGEN RV++WM R++G+ + +PIG
Sbjct: 497 WLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIG 556
Query: 178 LLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
+PK +N+ GL E+FS+ K + ++V++ K+L +Q DLP I +E+ +
Sbjct: 557 YIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALK 616
Query: 355 KRIHNM 372
+RI M
Sbjct: 617 QRISQM 622
>sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 114 bits (285), Expect = 1e-25
Identities = 54/126 (42%), Positives = 83/126 (65%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIG 177
WL + + P K+P IFHVNWFR + +GKF+WPGFGEN RV++WM R++G+ A+ +PIG
Sbjct: 497 WLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKAGAKLTPIG 556
Query: 178 LLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
+PK +N+ GL E+FS+ K + ++V++ K+L +Q DLP I +E+ +
Sbjct: 557 YIPKEDALNLKGLGHINVMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALK 616
Query: 355 KRIHNM 372
+RI M
Sbjct: 617 QRISQM 622
>sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 113 bits (283), Expect = 2e-25
Identities = 55/126 (43%), Positives = 79/126 (62%), Gaps = 2/126 (1%)
Frame = +1
Query: 1 WLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIG 177
WL + ++P K+P IFHVNWFR + G+F+WPGFGEN RV++WM RV GE A +PIG
Sbjct: 497 WLSMAQRPAAKLPKIFHVNWFRKDKAGRFLWPGFGENSRVLEWMFNRVVGEGGASVTPIG 556
Query: 178 LLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQE 354
+P +++ GL D E+F + K + E+V+E K+L EQ DLP I ++ +
Sbjct: 557 YIPDEDALDLRGLGDVDVKELFHISKEFWEEEVEEIQKYLEEQVNVDLPPEIKNQVLALK 616
Query: 355 KRIHNM 372
+RI M
Sbjct: 617 QRISQM 622
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,959,271
Number of Sequences: 369166
Number of extensions: 1299235
Number of successful extensions: 3190
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3099
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3164
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)