Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_L02
(347 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP... 74 8e-14
sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP... 74 1e-13
sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mi... 64 1e-10
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 60 2e-09
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 59 3e-09
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 53 2e-07
sp|P21642|PPCKM_CHICK Phosphoenolpyruvate carboxykinase, mi... 50 1e-06
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 50 2e-06
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 48 8e-06
sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cy... 48 8e-06
>sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 619
Score = 74.3 bits (181), Expect = 8e-14
Identities = 35/68 (51%), Positives = 48/68 (70%), Gaps = 1/68 (1%)
Frame = +2
Query: 11 SPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQEL 187
+PIG++PK G IN +GL D +WDE+ S+PK Y + D +E KFL EQ G DLP I E+
Sbjct: 549 TPIGIVPKKGAINASGLPDIQWDELMSVPKEYWTNDAKEIRKFLEEQVGPDLPKEIRAEM 608
Query: 188 NEQEKRIH 211
+ QE+RI+
Sbjct: 609 DAQEERIN 616
>sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 643
Score = 73.9 bits (180), Expect = 1e-13
Identities = 36/70 (51%), Positives = 46/70 (65%), Gaps = 1/70 (1%)
Frame = +2
Query: 2 AEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGII 178
AE +PIG +PK GTIN+ GL + WDE+ S+PK+Y ED+ ET F Q G DLP I
Sbjct: 569 AEETPIGFIPKKGTINLEGLPNVNWDELMSIPKSYWLEDMVETKTFFENQVGSDLPPEIA 628
Query: 179 QELNEQEKRI 208
+EL Q +RI
Sbjct: 629 KELEAQTERI 638
>sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 63.5 bits (153), Expect = 1e-10
Identities = 29/73 (39%), Positives = 48/73 (65%), Gaps = 1/73 (1%)
Frame = +2
Query: 2 AEPSPIGLLPKHGTINVTGLDA-KWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGII 178
A +PIGL+PK G ++++GL A ++FSLPK + ++V++ +L EQ +DLP ++
Sbjct: 568 ARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVL 627
Query: 179 QELNEQEKRIHNM 217
EL E+R+H M
Sbjct: 628 AELEALERRVHKM 640
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 59.7 bits (143), Expect = 2e-09
Identities = 29/71 (40%), Positives = 44/71 (61%), Gaps = 2/71 (2%)
Frame = +2
Query: 11 SPIGLLPKHGTINVTGLDAKWD--EMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQE 184
S IG +P G +N+ G+ K D E+FSLPK + S++V++ + Q G DLPA I Q+
Sbjct: 577 SAIGHIPAEGALNLDGMKDKVDVKEIFSLPKEFWSQEVKDIRTYFESQVGADLPASIYQQ 636
Query: 185 LNEQEKRIHNM 217
L+E R+ N+
Sbjct: 637 LDELSSRVDNL 647
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 59.3 bits (142), Expect = 3e-09
Identities = 27/73 (36%), Positives = 47/73 (64%), Gaps = 1/73 (1%)
Frame = +2
Query: 2 AEPSPIGLLPKHGTINVTGLDA-KWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGII 178
A+ +PIGL+PK G ++++GL A ++FS+PK + ++V++ +L EQ +DLP ++
Sbjct: 568 AQETPIGLVPKEGALDLSGLSAVDTSQLFSIPKDFWEQEVRDIRGYLTEQVNQDLPKEVL 627
Query: 179 QELNEQEKRIHNM 217
EL E R+ M
Sbjct: 628 AELEALEGRVQKM 640
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 52.8 bits (125), Expect = 2e-07
Identities = 27/73 (36%), Positives = 44/73 (60%), Gaps = 1/73 (1%)
Frame = +2
Query: 2 AEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGII 178
A+ +PIG +PK +N+ GL D +E+F + K + ++V+E K+L +Q DLP I
Sbjct: 550 AKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIE 609
Query: 179 QELNEQEKRIHNM 217
+EL ++RI M
Sbjct: 610 RELRALKQRISQM 622
>sp|P21642|PPCKM_CHICK Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 50.4 bits (119), Expect = 1e-06
Identities = 23/73 (31%), Positives = 42/73 (57%), Gaps = 1/73 (1%)
Frame = +2
Query: 2 AEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGII 178
A P+PIG +PK G +++ GL + ++F + K + E+ ++ ++ E G DLP ++
Sbjct: 568 ARPTPIGWVPKEGDLDLGGLPGVDYSQLFPMEKGFWEEECRQLREYYGENFGADLPRDVM 627
Query: 179 QELNEQEKRIHNM 217
EL E+R+ M
Sbjct: 628 AELEGLEERVRKM 640
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 49.7 bits (117), Expect = 2e-06
Identities = 25/73 (34%), Positives = 43/73 (58%), Gaps = 1/73 (1%)
Frame = +2
Query: 2 AEPSPIGLLPKHGTINVTGLDA-KWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGII 178
A+ +PIG +PK +N+ GL +E+F + K + ++V+E ++L +Q DLP I
Sbjct: 550 AKLTPIGYIPKENALNLKGLGGVNVEELFGISKEFWEKEVEEIDRYLEDQVNTDLPYEIE 609
Query: 179 QELNEQEKRIHNM 217
+EL ++RI M
Sbjct: 610 RELRALKQRISQM 622
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 47.8 bits (112), Expect = 8e-06
Identities = 24/70 (34%), Positives = 41/70 (58%), Gaps = 1/70 (1%)
Frame = +2
Query: 11 SPIGLLPKHGTINVTGLD-AKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQEL 187
+PIG +PK +N+ GL E+FS+ K + ++V++ K+L +Q DLP I +E+
Sbjct: 553 TPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREI 612
Query: 188 NEQEKRIHNM 217
++RI M
Sbjct: 613 LALKQRISQM 622
>sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 47.8 bits (112), Expect = 8e-06
Identities = 25/73 (34%), Positives = 43/73 (58%), Gaps = 1/73 (1%)
Frame = +2
Query: 2 AEPSPIGLLPKHGTINVTGLD-AKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGII 178
A+ +PIG +PK +N+ GL E+FS+ K + ++V++ K+L +Q DLP I
Sbjct: 550 AKLTPIGYIPKEDALNLKGLGHINVMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIE 609
Query: 179 QELNEQEKRIHNM 217
+E+ ++RI M
Sbjct: 610 REILALKQRISQM 622
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,816,148
Number of Sequences: 369166
Number of extensions: 690537
Number of successful extensions: 1499
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1467
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1491
length of database: 68,354,980
effective HSP length: 83
effective length of database: 53,021,975
effective search space used: 1696703200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)