Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_K18
(895 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q96QK1|VPS35_HUMAN Vacuolar protein sorting 35 (Vesicle ... 228 2e-59
sp|Q9EQH3|VPS35_MOUSE Vacuolar protein sorting 35 (Vesicle ... 226 7e-59
sp|O74552|VPS35_SCHPO Vacuolar protein sorting-associated p... 111 2e-24
sp|P34110|VPS35_YEAST Vacuolar protein sorting-associated p... 109 1e-23
sp|Q97WH0|RAD50_SULSO DNA double-strand break repair rad50 ... 33 1.1
sp|Q9ZD08|DNAB_RICPR Replicative DNA helicase 32 1.8
sp|P03752|VE47_LAMBD EA47 GENE PROTEIN 32 2.4
sp|P38170|CND2_YEAST Condensin complex subunit 2 (Barren ho... 32 3.1
sp|O75006|SEC15_SCHPO Exocyst complex component sec15 32 3.1
sp|Q9Z140|CPNE6_MOUSE Copine-6 (Copine VI) (Neuronal-copine... 30 9.1
>sp|Q96QK1|VPS35_HUMAN Vacuolar protein sorting 35 (Vesicle protein sorting 35) (hVPS35)
(Maternal-embryonic 3)
Length = 796
Score = 228 bits (581), Expect = 2e-59
Identities = 114/257 (44%), Positives = 168/257 (65%)
Frame = +3
Query: 27 PIVFQTLNLATRYHTQYDESEIWQRKLEKVFKLFHDCVSHFANNGAAELALRLFLQGALT 206
P+VF LA RY + W++K +K+F H +S AEL LRLFLQGAL
Sbjct: 531 PLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALA 590
Query: 207 IDRISKFEKRELLAYEFFAQAFSLFEEEIADSRAQINAIMLITSTLERVSCLSEDNHCAL 386
I FE E +AYEF +QAFSL+E+EI+DS+AQ+ AI LI T ER+ C SE+NH L
Sbjct: 591 AGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPL 649
Query: 387 RSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPIEEHGKELRDHKKLISCLDKSEKLAN 566
R+QCA A A +L+KK DQ RAV+ HLFWSG+ +++G+EL K+++ CL K+ K+AN
Sbjct: 650 RTQCALA-ASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIAN 708
Query: 567 ECLEVTMKAQLLIELLSRFLYFYNIDPENIPSLRINSLIRSIRKLLPDIQSGNDGESINN 746
+C++ +++ QL IE+L+R++YFY + + + +N LI+ IR+ LP+++S + E IN
Sbjct: 709 QCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINK 768
Query: 747 HFDNVISFIELKQRCAE 797
HF N + + L++ E
Sbjct: 769 HFHNTLEHLRLRRESPE 785
>sp|Q9EQH3|VPS35_MOUSE Vacuolar protein sorting 35 (Vesicle protein sorting 35)
(Maternal-embryonic 3)
Length = 796
Score = 226 bits (576), Expect = 7e-59
Identities = 113/257 (43%), Positives = 167/257 (64%)
Frame = +3
Query: 27 PIVFQTLNLATRYHTQYDESEIWQRKLEKVFKLFHDCVSHFANNGAAELALRLFLQGALT 206
P+VF LA RY + W++K +K+F H +S AEL LRLFLQGAL
Sbjct: 531 PLVFAAYQLAFRYKENSQMDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALA 590
Query: 207 IDRISKFEKRELLAYEFFAQAFSLFEEEIADSRAQINAIMLITSTLERVSCLSEDNHCAL 386
I FE E +AYEF +QAFSL+E+EI+DS+AQ+ AI LI T ER+ C SE+NH L
Sbjct: 591 AGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPL 649
Query: 387 RSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPIEEHGKELRDHKKLISCLDKSEKLAN 566
R+QCA A A +L+KK DQ RAV+ HLFWSG+ +++G+EL K+++ CL K+ K+AN
Sbjct: 650 RTQCALA-ASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIAN 708
Query: 567 ECLEVTMKAQLLIELLSRFLYFYNIDPENIPSLRINSLIRSIRKLLPDIQSGNDGESINN 746
+C++ +++ QL IE+L+R++YFY + + + +N LI+ IR+ LP+++S + E IN
Sbjct: 709 QCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINK 768
Query: 747 HFDNVISFIELKQRCAE 797
HF N + + ++ E
Sbjct: 769 HFHNTLEHLRSRRESPE 785
>sp|O74552|VPS35_SCHPO Vacuolar protein sorting-associated protein vps35
Length = 785
Score = 111 bits (278), Expect = 2e-24
Identities = 65/198 (32%), Positives = 109/198 (55%), Gaps = 1/198 (0%)
Frame = +3
Query: 93 WQRKLEKVFKLFHDCVSHFANNG-AAELALRLFLQGALTIDRISKFEKRELLAYEFFAQA 269
W K+ +++ + C++ NG + EL L L+L A D+ E AYEFF QA
Sbjct: 527 WAEKVRLLWEFVNTCINVLYKNGDSLELCLALYLSAAEMADQ----ENYPDFAYEFFTQA 582
Query: 270 FSLFEEEIADSRAQINAIMLITSTLERVSCLSEDNHCALRSQCAKAGALRLVKKMDQSRA 449
FS++EE + DS Q +++I L++ S D++ L ++C A +L+KK DQ
Sbjct: 583 FSIYEESVLDSELQYQQLLMIIGKLQKTRNFSVDDYDTLITKCTLY-ASKLLKKPDQCCG 641
Query: 450 VALAGHLFWSGKPIEEHGKELRDHKKLISCLDKSEKLANECLEVTMKAQLLIELLSRFLY 629
+ LA HL+W E+ + +D K+++ CL KS K+A+ C++ +L I +L R+ Y
Sbjct: 642 IYLASHLWWQVASGED-SRPFQDPKRVLECLQKSLKIADACMDQLTSLKLFINILERYFY 700
Query: 630 FYNIDPENIPSLRINSLI 683
+Y+ E+I + I+ LI
Sbjct: 701 YYDQHCESIIAKHISGLI 718
>sp|P34110|VPS35_YEAST Vacuolar protein sorting-associated protein VPS35
Length = 944
Score = 109 bits (272), Expect = 1e-23
Identities = 73/234 (31%), Positives = 130/234 (55%), Gaps = 15/234 (6%)
Frame = +3
Query: 105 LEKVFKLFHDCVSHF---ANNGAAELALRLFLQGALTIDRISKFEKRELLAYEFFAQAFS 275
L+++FK C++ NN +L L+L LQ A+ D++ E ++Y+FF+QAF+
Sbjct: 623 LKQMFKFVSRCINDIFNSCNNSCTDLILKLNLQCAILADQLQLNE----ISYDFFSQAFT 678
Query: 276 LFEEEIADSRAQINAIMLITSTLERV-SCLSEDNHCALRSQCAKAGALRLVKKMDQSRAV 452
+FEE ++DS+ Q+ A++ I +L++ S E + +L +C G+ +L+KK DQ RAV
Sbjct: 679 IFEESLSDSKTQLQALIYIAQSLQKTRSLYKEAYYDSLIVRCTLHGS-KLLKKQDQCRAV 737
Query: 453 ALAGHLFWSGKPIEEHGKE-------LRDHKKLISCLDKSEKLANECLEVTMKAQLLIEL 611
L HL+W+ + I G+E RD K+++ CL +S ++A+ ++ +L++E+
Sbjct: 738 YLCSHLWWATE-ISNIGEEEGITDNFYRDGKRVLECLQRSLRVADSIMDNEQSCELMVEI 796
Query: 612 LSRFLYFYNIDPE---NIPSLRINSLIRSIRKLLPDIQ-SGNDGESINNHFDNV 761
L+R LY++ E +I IN LI I+ L ++ N I N ++
Sbjct: 797 LNRCLYYFIHGDESETHISIKYINGLIELIKTNLKSLKLEDNSASMITNSISDL 850
>sp|Q97WH0|RAD50_SULSO DNA double-strand break repair rad50 ATPase
Length = 864
Score = 33.1 bits (74), Expect = 1.1
Identities = 38/168 (22%), Positives = 77/168 (45%), Gaps = 19/168 (11%)
Frame = +3
Query: 213 RISKFEKRELLAYEFFAQAFSLFEEEIADSRAQINAIMLITSTLERVSCLSEDNHCALRS 392
++ + +K++ + ++ Q L +EE+ + R ++N I E+ + L++
Sbjct: 595 KLDEMKKKKGILEDYIRQV-KLLQEEVKNLREEVNIIQF-----------DENRYNELKT 642
Query: 393 QCAKAGALRLVKKMDQSRAVALAGHLFWSGKPIEE-------HGKELRDHKKLISCLDKS 551
A L K ++R + G L K IEE + +L+D +K+I+ ++K
Sbjct: 643 SL---DAYNLSLKEKENRKSRIEGELESLEKDIEEISNRIANYELQLKDREKIINAINKL 699
Query: 552 EKLANECLE------VTMKAQLLIE-----LLSRF-LYFYNIDPENIP 659
EK+ + E + M + LIE ++S+F L N++ E +P
Sbjct: 700 EKIRSALGERKLQSYIIMTTKQLIENNLNDIISKFDLSIKNVEMEIMP 747
>sp|Q9ZD08|DNAB_RICPR Replicative DNA helicase
Length = 486
Score = 32.3 bits (72), Expect = 1.8
Identities = 31/123 (25%), Positives = 55/123 (44%), Gaps = 2/123 (1%)
Frame = +3
Query: 330 ITSTLERVSCLSEDNHCALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPIEEH--G 503
I ST R LSED + LR + L+ +D + A++++ + + +H G
Sbjct: 279 IDSTALRTGMLSEDKYNRLRKEANTLSELQFF--IDDTPALSISAIRTRARRMKRKHNLG 336
Query: 504 KELRDHKKLISCLDKSEKLANECLEVTMKAQLLIELLSRFLYFYNIDPENIPSLRINSLI 683
D+ +LI + KSE NE E+T + + + L NIP + ++ L
Sbjct: 337 ILFIDYLQLIRGVSKSENRVNEISEITQGLKAIAKEL------------NIPVIALSQLS 384
Query: 684 RSI 692
R++
Sbjct: 385 RAV 387
>sp|P03752|VE47_LAMBD EA47 GENE PROTEIN
Length = 410
Score = 32.0 bits (71), Expect = 2.4
Identities = 15/56 (26%), Positives = 29/56 (51%)
Frame = +3
Query: 93 WQRKLEKVFKLFHDCVSHFANNGAAELALRLFLQGALTIDRISKFEKRELLAYEFF 260
W+R+L+ + L C+ + + L L FLQ A T+ I + K +++ Y+ +
Sbjct: 6 WERRLKDLSHLLKCCIDTYFDPELFRLNLNQFLQTARTVTFIIQKNKNQIIGYDIW 61
>sp|P38170|CND2_YEAST Condensin complex subunit 2 (Barren homolog) (CAPH homolog)
Length = 728
Score = 31.6 bits (70), Expect = 3.1
Identities = 23/67 (34%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
Frame = +3
Query: 606 ELLSRFLYFYNIDPENIPSLRINSLIRSIRKLLPDIQSGNDG-ESINNHFDNVISFIELK 782
E+LS + F D I I+ I +R ++ DI D E++NN FDN ++ EL
Sbjct: 253 EILSLGMDFIKFD--QIAVCEISGSIEQLRNVVEDINQAKDFIENVNNRFDNFLTEEEL- 309
Query: 783 QRCAEDN 803
Q DN
Sbjct: 310 QAAVPDN 316
>sp|O75006|SEC15_SCHPO Exocyst complex component sec15
Length = 785
Score = 31.6 bits (70), Expect = 3.1
Identities = 34/143 (23%), Positives = 58/143 (40%)
Frame = +3
Query: 264 QAFSLFEEEIADSRAQINAIMLITSTLERVSCLSEDNHCALRSQCAKAGALRLVKKMDQS 443
Q SL E+ +++ L + + RV LS +N+ + +R + QS
Sbjct: 27 QLISLACEKGQETQVYERLNELASKSAIRVEQLSAENNQDFSKSVNQLSVVRSELRSLQS 86
Query: 444 RAVALAGHLFWSGKPIEEHGKELRDHKKLISCLDKSEKLANECLEVTMKAQLLIELLSRF 623
L + SG+ ++ ++L L K L C++V + Q ELLS+
Sbjct: 87 LMADLNTDIQASGRDLQNMKQQLNSLSTSERFLTKYHNLVRSCMQVLHQVQYSQELLSKK 146
Query: 624 LYFYNIDPENIPSLRINSLIRSI 692
Y +P+LRI S I +
Sbjct: 147 QY--------LPTLRICSEISEV 161
>sp|Q9Z140|CPNE6_MOUSE Copine-6 (Copine VI) (Neuronal-copine) (N-copine)
Length = 557
Score = 30.0 bits (66), Expect = 9.1
Identities = 22/66 (33%), Positives = 29/66 (43%)
Frame = +3
Query: 636 NIDPENIPSLRINSLIRSIRKLLPDIQSGNDGESINNHFDNVISFIELKQRCAEDNNATK 815
N DPEN I+ +I S R+ LP IQ N + E QR ATK
Sbjct: 379 NFDPENPECEEISGVIASYRRCLPQIQLYGP----TNVAPIINRVAEPAQREQSTGQATK 434
Query: 816 FSQLMI 833
+S L++
Sbjct: 435 YSVLLV 440
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,352,792
Number of Sequences: 369166
Number of extensions: 1749167
Number of successful extensions: 4597
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4472
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4589
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8982382310
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)