Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_K06
(681 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 134 2e-31
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 133 5e-31
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 131 1e-30
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 129 5e-30
sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (... 129 5e-30
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 129 5e-30
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 129 9e-30
sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (S... 127 2e-29
sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (S... 127 4e-29
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 126 6e-29
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 134 bits (337), Expect = 2e-31
Identities = 78/198 (39%), Positives = 120/198 (60%), Gaps = 1/198 (0%)
Frame = +1
Query: 91 NVEPDFENL-VKLEKLGKGSFGTVFKCVSIETKGEMAVKKLKIPTADKTKEEKLSVEREL 267
N++ D E L KLEK+GKGSFG VFK + T+ +A+K + + A+ E+ +++E+
Sbjct: 27 NLKADPEELFTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIED---IQQEI 83
Query: 268 QVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQIL 447
V S+ D P + KY ++ + ++++MEY+ GGS + GPL E +I ++IL
Sbjct: 84 TVLSQ-CDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPLDETQIATILREIL 141
Query: 448 EALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTP 627
+ L YLH +K +HRDIK+ N+LL E VKL DFG++ LTD K + VGTP
Sbjct: 142 KGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKR------NTFVGTP 195
Query: 628 NYMAPDIMNEEPYDSSAD 681
+MAP+++ + YDS AD
Sbjct: 196 FWMAPEVIKQSAYDSKAD 213
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 133 bits (334), Expect = 5e-31
Identities = 78/198 (39%), Positives = 120/198 (60%), Gaps = 1/198 (0%)
Frame = +1
Query: 91 NVEPDFENL-VKLEKLGKGSFGTVFKCVSIETKGEMAVKKLKIPTADKTKEEKLSVEREL 267
N++ D E L KLEK+GKGSFG VFK + T+ +A+K + + A+ E+ +++E+
Sbjct: 15 NLKADPEELFTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIED---IQQEI 71
Query: 268 QVYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQIL 447
V S+ D P + KY ++ + ++++MEY+ GGS + GPL E +I ++IL
Sbjct: 72 TVLSQ-CDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPLDEIQIATILREIL 129
Query: 448 EALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTP 627
+ L YLH +K +HRDIK+ N+LL E VKL DFG++ LTD K + VGTP
Sbjct: 130 KGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKR------NTFVGTP 183
Query: 628 NYMAPDIMNEEPYDSSAD 681
+MAP+++ + YDS AD
Sbjct: 184 FWMAPEVIKQSAYDSKAD 201
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 131 bits (330), Expect = 1e-30
Identities = 77/187 (41%), Positives = 117/187 (62%), Gaps = 4/187 (2%)
Frame = +1
Query: 133 LGKGSFGTVFKCVSIETKGEMAVKKLKI-PTADKTKEEKLSVERELQVYSKHSDHPRIVK 309
LG+G+FG V+ C ++T E+AVK+++ P + +T +E ++E E+Q+ K+ H RIV+
Sbjct: 361 LGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLL-KNFLHERIVQ 419
Query: 310 YERNFKT--EDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILEALQYLHEQKIL 483
Y + E + + MEYM GGS+ ++ G L+EN +KY++QILE + YLH IL
Sbjct: 420 YYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYLHSNMIL 479
Query: 484 HRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNG-AIQSSVGTPNYMAPDIMNEE 660
HRDIK NIL D NVKL DFG SK L + L+G ++S GTP +M+P++++ +
Sbjct: 480 HRDIKGANILRDSTGNVKLGDFGASKRLQTIC----LSGTGMKSVTGTPYWMSPEVISGQ 535
Query: 661 PYDSSAD 681
Y AD
Sbjct: 536 GYGRKAD 542
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 129 bits (325), Expect = 5e-30
Identities = 74/186 (39%), Positives = 113/186 (60%), Gaps = 3/186 (1%)
Frame = +1
Query: 133 LGKGSFGTVFKCVSIETKGEMAVKKLKI-PTADKTKEEKLSVERELQVYSKHSDHPRIVK 309
LG+G+FG V+ C ++T E+A K+++ P + +T +E ++E E+Q+ K+ H RIV+
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLL-KNLQHERIVQ 426
Query: 310 YERNFK--TEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILEALQYLHEQKIL 483
Y + E + + MEYM GGS+ ++ G L+E+ +KY++QILE + YLH I+
Sbjct: 427 YYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIV 486
Query: 484 HRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTPNYMAPDIMNEEP 663
HRDIK NIL D NVKL DFG SK L + I+S GTP +M+P++++ E
Sbjct: 487 HRDIKGANILRDSAGNVKLGDFGASKRLQTICMS---GTGIRSVTGTPYWMSPEVISGEG 543
Query: 664 YDSSAD 681
Y AD
Sbjct: 544 YGRKAD 549
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
(Serine/threonine-protein kinase MASK) (Mst3 and
SOK1-related kinase)
Length = 416
Score = 129 bits (325), Expect = 5e-30
Identities = 75/197 (38%), Positives = 116/197 (58%)
Frame = +1
Query: 91 NVEPDFENLVKLEKLGKGSFGTVFKCVSIETKGEMAVKKLKIPTADKTKEEKLSVERELQ 270
N+ E KLE++GKGSFG VFK + T+ +A+K + + A+ E+ +++E+
Sbjct: 16 NIADPEELFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIED---IQQEIT 72
Query: 271 VYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILE 450
V S+ D + KY ++ ++++MEY+ GGS +R GP E +I K+IL+
Sbjct: 73 VLSQ-CDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRA-GPFDEFQIATMLKEILK 130
Query: 451 ALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTPN 630
L YLH +K +HRDIK+ N+LL E +VKL DFG++ LTD K + VGTP
Sbjct: 131 GLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKR------NTFVGTPF 184
Query: 631 YMAPDIMNEEPYDSSAD 681
+MAP+++ + YDS AD
Sbjct: 185 WMAPEVIQQSAYDSKAD 201
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 129 bits (325), Expect = 5e-30
Identities = 75/197 (38%), Positives = 116/197 (58%)
Frame = +1
Query: 91 NVEPDFENLVKLEKLGKGSFGTVFKCVSIETKGEMAVKKLKIPTADKTKEEKLSVERELQ 270
N+ E KLE++GKGSFG VFK + T+ +A+K + + A+ E+ +++E+
Sbjct: 16 NIADPEELFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIED---IQQEIT 72
Query: 271 VYSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILE 450
V S+ D + KY ++ ++++MEY+ GGS +R GP E +I K+IL+
Sbjct: 73 VLSQ-CDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRA-GPFDEFQIATMLKEILK 130
Query: 451 ALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTPN 630
L YLH +K +HRDIK+ N+LL E +VKL DFG++ LTD K + VGTP
Sbjct: 131 GLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKR------NTFVGTPF 184
Query: 631 YMAPDIMNEEPYDSSAD 681
+MAP+++ + YDS AD
Sbjct: 185 WMAPEVIQQSAYDSKAD 201
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 129 bits (323), Expect = 9e-30
Identities = 73/186 (39%), Positives = 113/186 (60%), Gaps = 3/186 (1%)
Frame = +1
Query: 133 LGKGSFGTVFKCVSIETKGEMAVKKLKI-PTADKTKEEKLSVERELQVYSKHSDHPRIVK 309
LG+G+FG V+ C ++T E+A K+++ P + +T +E ++E E+Q+ K+ H RIV+
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLL-KNLQHERIVQ 426
Query: 310 YERNFK--TEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILEALQYLHEQKIL 483
Y + E + + MEYM GGS+ ++ G L+E+ +KY++QILE + YLH I+
Sbjct: 427 YYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIV 486
Query: 484 HRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTPNYMAPDIMNEEP 663
HRDIK NIL D NVKL DFG SK L + ++S GTP +M+P++++ E
Sbjct: 487 HRDIKGANILRDSAGNVKLGDFGASKRLQTICMS---GTGMRSVTGTPYWMSPEVISGEG 543
Query: 664 YDSSAD 681
Y AD
Sbjct: 544 YGRKAD 549
>sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (Sterile 20/oxidant
stress-response kinase 1) (Ste20/oxidant stress response
kinase-1) (SOK-1) (Ste20-like kinase)
Length = 426
Score = 127 bits (320), Expect = 2e-29
Identities = 74/196 (37%), Positives = 118/196 (60%)
Frame = +1
Query: 94 VEPDFENLVKLEKLGKGSFGTVFKCVSIETKGEMAVKKLKIPTADKTKEEKLSVERELQV 273
V+P+ E KL+++GKGSFG V+K + TK +A+K + + A+ E+ +++E+ V
Sbjct: 14 VDPE-ELFTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIED---IQQEITV 69
Query: 274 YSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILEA 453
S+ D P I +Y ++ ++++MEY+ GGS + K GPL E I ++IL+
Sbjct: 70 LSQ-CDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLL-KPGPLEETYIATILREILKG 127
Query: 454 LQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTPNY 633
L YLH ++ +HRDIK+ N+LL E +VKL DFG++ LTD K + VGTP +
Sbjct: 128 LDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKR------NTFVGTPFW 181
Query: 634 MAPDIMNEEPYDSSAD 681
MAP+++ + YD AD
Sbjct: 182 MAPEVIKQSAYDFKAD 197
>sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (Sterile 20/oxidant
stress-response kinase 1) (Ste20/oxidant stress response
kinase-1) (SOK-1) (Ste20-like kinase)
Length = 426
Score = 127 bits (318), Expect = 4e-29
Identities = 73/196 (37%), Positives = 118/196 (60%)
Frame = +1
Query: 94 VEPDFENLVKLEKLGKGSFGTVFKCVSIETKGEMAVKKLKIPTADKTKEEKLSVERELQV 273
V+P+ E KL+++GKGSFG V+K + TK +A+K + + A+ E+ +++E+ V
Sbjct: 14 VDPE-ELFTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIED---IQQEITV 69
Query: 274 YSKHSDHPRIVKYERNFKTEDAIFLVMEYMKGGSLGGYIRKNGPLSENKIKKYSKQILEA 453
S+ D P I +Y ++ ++++MEY+ GGS + K GPL E I ++IL+
Sbjct: 70 LSQ-CDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLL-KPGPLEETYIATILREILKG 127
Query: 454 LQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLSKILTDLAAKDFLNGAIQSSVGTPNY 633
L YLH ++ +HRDIK+ N+LL E +VK+ DFG++ LTD K + VGTP +
Sbjct: 128 LDYLHSERKIHRDIKAANVLLSEQGDVKMADFGVAGQLTDTQIKR------NTFVGTPFW 181
Query: 634 MAPDIMNEEPYDSSAD 681
MAP+++ + YD AD
Sbjct: 182 MAPEVIKQSAYDFKAD 197
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 126 bits (316), Expect = 6e-29
Identities = 84/222 (37%), Positives = 127/222 (57%), Gaps = 4/222 (1%)
Frame = +1
Query: 28 PIESDNDIPKPSRTDDLQLYFNVEPDFENLVKLEKLGKGSFGTVFKCVSIETKGEMAVKK 207
P + DI PSR+ P L KL LG+G+FG V+ C ++T E+AVK+
Sbjct: 336 PTLTVTDISPPSRSPRA-------PTNWRLGKL--LGQGAFGRVYLCYDVDTGRELAVKQ 386
Query: 208 LKI-PTADKTKEEKLSVERELQVYSKHSDHPRIVKYERNFKT--EDAIFLVMEYMKGGSL 378
++ P + +T +E ++E E+Q+ K+ H RIV+Y + E + + ME GGS+
Sbjct: 387 VQFNPESPETSKEVNALECEIQLL-KNLLHERIVQYYGCLRDPQEKTLSIFMELSPGGSI 445
Query: 379 GGYIRKNGPLSENKIKKYSKQILEALQYLHEQKILHRDIKSDNILLDEDDNVKLCDFGLS 558
++ G L+EN +KY++QILE + YLH I+HRDIK NIL D N+KL DFG S
Sbjct: 446 KDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNIKLGDFGAS 505
Query: 559 KILTDLAAKDFLNG-AIQSSVGTPNYMAPDIMNEEPYDSSAD 681
K L + L+G ++S GTP +M+P++++ E Y AD
Sbjct: 506 KRLQTIC----LSGTGMKSVTGTPYWMSPEVISGEGYGRKAD 543
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.316 0.136 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,862,593
Number of Sequences: 369166
Number of extensions: 1507935
Number of successful extensions: 8551
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6077
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5782011865
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)