Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_J13
(574 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 57 3e-08
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 56 6e-08
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 55 2e-07
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 52 8e-07
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 51 2e-06
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 38 0.021
sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protei... 34 0.30
sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62) 32 0.86
sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase... 31 2.5
sp|Q9UPT9|UBP22_HUMAN Ubiquitin carboxyl-terminal hydrolase... 31 2.5
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 57.0 bits (136), Expect = 3e-08
Identities = 34/128 (26%), Positives = 52/128 (40%), Gaps = 2/128 (1%)
Frame = +3
Query: 45 VQINRSKKTSNCRLCCKFYNYL-QTISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRN 221
V++ K C +C K YL + + S+ +L L C+ + R +C +
Sbjct: 399 VRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTE 458
Query: 222 FNAVIAAFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAV 398
+ V+ F C C K LG C WGP +WC + + A C AV
Sbjct: 459 YEPVLIEILVEVMDPSFVCLKIGACPAAHKPLLGAEKCVWGPSYWCQNMESAALC--NAV 516
Query: 399 KHCEMNVW 422
+HC +VW
Sbjct: 517 EHCRRHVW 524
Score = 36.6 bits (83), Expect = 0.046
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +3
Query: 315 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
LG CT G WC + K A C GAV+HC VW
Sbjct: 20 LGLRECTRGSAVWCQNVKTAADC--GAVQHCLQTVW 53
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 56.2 bits (134), Expect = 6e-08
Identities = 33/128 (25%), Positives = 53/128 (41%), Gaps = 2/128 (1%)
Frame = +3
Query: 45 VQINRSKKTSNCRLCCKFYNYL-QTISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRN 221
V + + K C +C K YL + + S+ +L L C+ + + +C +
Sbjct: 398 VHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAE 457
Query: 222 FNAVIAAFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAV 398
+ V+ F C C K LG C WGP +WC + + A C AV
Sbjct: 458 YEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQC--NAV 515
Query: 399 KHCEMNVW 422
+HC+ +VW
Sbjct: 516 EHCKRHVW 523
Score = 37.7 bits (86), Expect = 0.021
Identities = 18/38 (47%), Positives = 20/38 (52%)
Frame = +3
Query: 315 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVWRQ 428
LG CT G WC + K A C GAVKHC VW +
Sbjct: 20 LGLKECTRGSAVWCQNVKTASDC--GAVKHCLQTVWNK 55
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 54.7 bits (130), Expect = 2e-07
Identities = 31/117 (26%), Positives = 50/117 (42%), Gaps = 2/117 (1%)
Frame = +3
Query: 78 CRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKS 254
C +C K YL+ + S+ +L L C+ + + +C + + ++
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498
Query: 255 KTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
F C +C K LG C WGPG+WC + + A C AV HC+ +VW
Sbjct: 499 VMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNSETAARC--NAVDHCKRHVW 553
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 52.4 bits (124), Expect = 8e-07
Identities = 29/117 (24%), Positives = 50/117 (42%), Gaps = 2/117 (1%)
Frame = +3
Query: 78 CRLCCKFYNYLQTISSKSSHSNLLFGLVS-FCNQISAHERLECLKQFRNFNAVIAAFNKS 254
C +C Y K++ + + L+ C+ + +C++ + V+
Sbjct: 403 CDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAE 462
Query: 255 KTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
F C +C +K LG++ C WGPG+WC + + A C AV HC +VW
Sbjct: 463 MMDPTFVCTKLGVCGAAKKPLLGEDACVWGPGYWCKNMETAAQC--NAVDHCRRHVW 517
Score = 40.8 bits (94), Expect = 0.002
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = +3
Query: 315 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
L + C GP WC S + A CG AVKHC+ NVW
Sbjct: 21 LWQKDCAKGPEVWCQSLRTASQCG--AVKHCQQNVW 54
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 50.8 bits (120), Expect = 2e-06
Identities = 30/117 (25%), Positives = 48/117 (41%), Gaps = 2/117 (1%)
Frame = +3
Query: 78 CRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKS 254
C +C K YL+ + S+ +L L C+ + + +C + ++
Sbjct: 442 CEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVE 501
Query: 255 KTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
F C +C K LG C WGP +WC + + A C AV HC+ +VW
Sbjct: 502 VMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVDHCKRHVW 556
Score = 31.2 bits (69), Expect = 1.9
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +3
Query: 330 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
C+ G C K AV CG AVKHC+ VW
Sbjct: 25 CSGGSAVLCRDVKTAVDCG--AVKHCQQMVW 53
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 37.7 bits (86), Expect = 0.021
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +3
Query: 330 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
C GP FWC S +HAV C A+ HC VW
Sbjct: 31 CAQGPQFWCQSLEHAVQC--RALGHCLQEVW 59
>sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (Pulmonary surfactant protein 18) (SP 18)
Length = 363
Score = 33.9 bits (76), Expect = 0.30
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +3
Query: 330 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 422
C GP FWC S + A+ C A+ HC VW
Sbjct: 25 CARGPAFWCQSLEQALQC--RALGHCLQEVW 53
>sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62)
Length = 416
Score = 32.3 bits (72), Expect = 0.86
Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = +3
Query: 78 CRLCCKFYNYLQTISSKSSHSNLLFGLVSFCNQ---ISAHERLECLKQFRNFNAVIAAFN 248
C +C ++ I K+S S FG++S CN +S + C KQF N +I +
Sbjct: 238 CSIC------MEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFE--NPIIKSCP 289
Query: 249 KSKTINEF 272
+ + I+EF
Sbjct: 290 ECRVISEF 297
>sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 2.5
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 494 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 384
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
>sp|Q9UPT9|UBP22_HUMAN Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 2.5
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 494 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 384
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,754,026
Number of Sequences: 369166
Number of extensions: 1320650
Number of successful extensions: 3378
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3268
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3373
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4226258440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)