Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_I23
(748 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q07050|TOP1_CRIGR DNA topoisomerase I 49 2e-05
sp|Q04750|TOP1_MOUSE DNA topoisomerase I 49 2e-05
sp|Q9WUL0|TOP1_RAT DNA topoisomerase I 49 2e-05
sp|P11387|TOP1_HUMAN DNA topoisomerase I 47 7e-05
sp|Q7YR26|TOP1_CERAE DNA topoisomerase I 47 7e-05
sp|P30189|TOP1_DROME DNA topoisomerase I 40 0.009
sp|Q969P6|TOP1M_HUMAN DNA topoisomerase I, mitochondrial pr... 38 0.025
sp|P04786|TOP1_YEAST DNA topoisomerase I 33 0.62
sp|P30181|TOP1_ARATH DNA topoisomerase I 32 1.4
sp|P93119|TOP1_DAUCA DNA topoisomerase I 32 1.8
>sp|Q07050|TOP1_CRIGR DNA topoisomerase I
Length = 767
Score = 48.5 bits (114), Expect = 2e-05
Identities = 19/28 (67%), Positives = 24/28 (85%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
VP+ KIYNKTQ +KF WA+DMT +DY+F
Sbjct: 740 VPIEKIYNKTQREKFAWAIDMTDEDYEF 767
>sp|Q04750|TOP1_MOUSE DNA topoisomerase I
Length = 767
Score = 48.5 bits (114), Expect = 2e-05
Identities = 19/28 (67%), Positives = 24/28 (85%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
VP+ KIYNKTQ +KF WA+DMT +DY+F
Sbjct: 740 VPIEKIYNKTQREKFAWAIDMTDEDYEF 767
>sp|Q9WUL0|TOP1_RAT DNA topoisomerase I
Length = 767
Score = 48.5 bits (114), Expect = 2e-05
Identities = 19/28 (67%), Positives = 24/28 (85%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
VP+ KIYNKTQ +KF WA+DMT +DY+F
Sbjct: 740 VPIEKIYNKTQREKFAWAIDMTDEDYEF 767
>sp|P11387|TOP1_HUMAN DNA topoisomerase I
Length = 765
Score = 46.6 bits (109), Expect = 7e-05
Identities = 18/28 (64%), Positives = 23/28 (82%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
VP+ KIYNKTQ +KF WA+DM +DY+F
Sbjct: 738 VPIEKIYNKTQREKFAWAIDMADEDYEF 765
>sp|Q7YR26|TOP1_CERAE DNA topoisomerase I
Length = 767
Score = 46.6 bits (109), Expect = 7e-05
Identities = 18/28 (64%), Positives = 23/28 (82%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
VP+ KIYNKTQ +KF WA+DM +DY+F
Sbjct: 740 VPIEKIYNKTQREKFAWAIDMADEDYEF 767
>sp|P30189|TOP1_DROME DNA topoisomerase I
Length = 972
Score = 39.7 bits (91), Expect = 0.009
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
VP+ KI+NKTQ KF WAV M ++Y+F
Sbjct: 945 VPIEKIFNKTQRTKFLWAVHMADENYRF 972
>sp|Q969P6|TOP1M_HUMAN DNA topoisomerase I, mitochondrial precursor (TOP1mt)
Length = 601
Score = 38.1 bits (87), Expect = 0.025
Identities = 14/29 (48%), Positives = 23/29 (79%)
Frame = +1
Query: 1 KVPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
+VP+ KIY+KTQ ++F WA+ M +D++F
Sbjct: 573 RVPVEKIYSKTQRERFAWALAMAGEDFEF 601
>sp|P04786|TOP1_YEAST DNA topoisomerase I
Length = 769
Score = 33.5 bits (75), Expect = 0.62
Identities = 11/28 (39%), Positives = 21/28 (75%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
VP+ KI+ KT +KF+WA++ ++++F
Sbjct: 742 VPIEKIFTKTLREKFKWAIESVDENWRF 769
>sp|P30181|TOP1_ARATH DNA topoisomerase I
Length = 916
Score = 32.3 bits (72), Expect = 1.4
Identities = 13/29 (44%), Positives = 22/29 (75%)
Frame = +1
Query: 1 KVPLNKIYNKTQLQKFQWAVDMTTKDYKF 87
+VP+ KI+ K+ L+KF WA+D+ +Y+F
Sbjct: 886 EVPIEKIFTKSLLEKFAWAMDV-EPEYRF 913
>sp|P93119|TOP1_DAUCA DNA topoisomerase I
Length = 790
Score = 32.0 bits (71), Expect = 1.8
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +1
Query: 4 VPLNKIYNKTQLQKFQWAVDM 66
VP+ KI+NK+ L KF WA+D+
Sbjct: 764 VPIEKIFNKSLLAKFAWAMDV 784
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,707,176
Number of Sequences: 369166
Number of extensions: 777106
Number of successful extensions: 1907
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1883
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1906
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6776504000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)