Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_G22
(839 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P27636|CDC15_YEAST Cell division control protein 15 113 6e-25
sp|P32491|MKK2_YEAST MAP kinase kinase MKK2/SSP33 101 3e-21
sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2 97 4e-20
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 97 6e-20
sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32 97 6e-20
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 97 7e-20
sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (S... 96 9e-20
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 96 1e-19
sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1 96 1e-19
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 96 2e-19
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 113 bits (283), Expect = 6e-25
Identities = 75/247 (30%), Positives = 127/247 (51%), Gaps = 9/247 (3%)
Frame = +2
Query: 107 RKVIGKGLSRIILLKNNETREQIV-LKKPYCEQGEIYRDICIQNKLSSSKNLKIYSKLYL 283
++VIG+G ++ N+ +Q+V +K+ E E DI + +S KNL ++ +
Sbjct: 28 KQVIGRGSYGVVYKAINKHTDQVVAIKEVVYENDEELNDIMAE--ISLLKNLN-HNNIVK 84
Query: 284 FENFI------CVGMEYIPNESLDSYLK--TTDLSEKEILKIAYQILIQIEELHKFKIVH 439
+ FI + +EY N SL + +T LSE E Q L+ ++ LH ++H
Sbjct: 85 YHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEGVIH 144
Query: 440 CDIKCGNVCIDKDGNAKIIDFGLSKDISDLSWVFSGTLRYCAPEIIKHENLSFGIDIWAF 619
DIK N+ + D K+ DFG+S ++ + +GTL + APEI+ + S DIW+
Sbjct: 145 RDIKAANILLSADNTVKLADFGVSTIVNSSALTLAGTLNWMAPEILGNRGASTLSDIWSL 204
Query: 620 GMLLVEIVTKNIPFHQIKNNRFIFEIRSMKKYEVKPIISISKEFLQFIERCLTISSSERP 799
G +VE++TKN P+H + + + + + Y P S S+ F+ +C + +RP
Sbjct: 205 GATVVEMLTKNPPYHNLTDANIYYAVENDTYY---PPSSFSEPLKDFLSKCFVKNMYKRP 261
Query: 800 TAMQLLE 820
TA QLL+
Sbjct: 262 TADQLLK 268
>sp|P32491|MKK2_YEAST MAP kinase kinase MKK2/SSP33
Length = 506
Score = 101 bits (251), Expect = 3e-21
Identities = 73/224 (32%), Positives = 120/224 (53%), Gaps = 17/224 (7%)
Frame = +2
Query: 200 QGEIYRDICIQNKLSSSKNLKIYSKLYLFE--NFICVGMEYIPNESLDSYLKTT-----D 358
Q +I+R++ NK S + Y ++ E + I + MEY+ +SL++ K
Sbjct: 255 QKQIFRELQF-NKSFKSDYIVQYYGMFTDEQSSSIYIAMEYMGGKSLEATYKNLLKRGGR 313
Query: 359 LSEKEILKIAYQILIQIEELHKFKIVHCDIKCGNVCIDKDGNAKIIDFGLSKD-ISDLSW 535
+SE+ I KIA +L + LH+ K++H DIK N+ +++ G K+ DFG+S + ++ L+
Sbjct: 314 ISERVIGKIAESVLRGLSYLHERKVIHRDIKPQNILLNEKGEIKLCDFGVSGEAVNSLAM 373
Query: 536 VFSGTLRYCAPEIIKHENLSFGIDIWAFGMLLVEIVTKNIPFH--QIKNNRFIFEIRSM- 706
F+GT Y APE I+ + S D+W+ G+ L+E+ PF +I N E+ +M
Sbjct: 374 TFTGTSFYMAPERIQGQPYSVTCDVWSLGLTLLEVAGGRFPFESDKITQNVAPIELLTMI 433
Query: 707 ------KKYEVKPIISISKEFLQFIERCLTISSSERPTAMQLLE 820
K E + IS SK F FI+ CL + ERP+ Q+L+
Sbjct: 434 LTFSPQLKDEPELDISWSKTFRSFIDYCLKKDARERPSPRQMLK 477
>sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2
Length = 589
Score = 97.4 bits (241), Expect = 4e-20
Identities = 60/192 (31%), Positives = 97/192 (50%), Gaps = 4/192 (2%)
Frame = +2
Query: 254 NLKIYSKLYLF-ENFICVGMEYIPNESLDSYLKTTDLSEKEILKIAYQILIQIEELHKFK 430
N+ Y +L E + V MEY+ SL ++ + L+E +I +I + I+ LH
Sbjct: 370 NIVTYLDSFLVRERHLWVVMEYMNAGSLTDIIEKSKLTEAQIARICLETCKGIQHLHARN 429
Query: 431 IVHCDIKCGNVCIDKDGNAKIIDFGLSKDISDLS---WVFSGTLRYCAPEIIKHENLSFG 601
I+H DIK NV +D GN KI DFG +S+ + GT + APE++K
Sbjct: 430 IIHRDIKSDNVLLDNSGNIKITDFGFCARLSNRTNKRVTMVGTPYWMAPEVVKQNEYGTK 489
Query: 602 IDIWAFGMLLVEIVTKNIPFHQIKNNRFIFEIRSMKKYEVKPIISISKEFLQFIERCLTI 781
+DIW+ G++++E++ P+ + R ++ I +K +SK F+ CLTI
Sbjct: 490 VDIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLKKPNLVSKNLKSFLNSCLTI 549
Query: 782 SSSERPTAMQLL 817
+ R TA +LL
Sbjct: 550 DTIFRATAAELL 561
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 97.1 bits (240), Expect = 6e-20
Identities = 61/178 (34%), Positives = 94/178 (52%), Gaps = 7/178 (3%)
Frame = +2
Query: 308 MEYIPNESLDSYL-KTTDLSEKEILKIAYQILIQIEELHKFKIVHCDIKCGNVCIDKDGN 484
+E++P S+ S L K E + Q+L+ +E LH I+H DIK N+ +D G
Sbjct: 150 LEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGC 209
Query: 485 AKIIDFGLSKDISDLSWV-----FSGTLRYCAPEIIKHENLSFGIDIWAFGMLLVEIVTK 649
K+ DFG SK +++L+ + GT + APE+I SF DIW+ G ++E+VT
Sbjct: 210 IKLADFGASKQVAELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTG 269
Query: 650 NIPF-HQIKNNRFIFEIRSMKKYEVKPIISISKEFLQFIERCLTISSSERPTAMQLLE 820
P+ Q K IF I + K + P +IS + F+ +CL + RPTA +LL+
Sbjct: 270 KAPWSQQYKEIAAIFHIGTTKSHPPIP-DNISSDANDFLLKCLQQEPNLRPTASELLK 326
>sp|P32490|MKK1_YEAST MAP kinase kinase MKK1/SSP32
Length = 508
Score = 97.1 bits (240), Expect = 6e-20
Identities = 67/222 (30%), Positives = 109/222 (49%), Gaps = 16/222 (7%)
Frame = +2
Query: 200 QGEIYRDICIQNKLSSSKNLKIYSKLYLFENF-ICVGMEYIPNESLDSYLKTT-----DL 361
Q +I+R++ S ++ Y EN I + MEY+ SLD+ K +
Sbjct: 262 QKQIFRELQFNRSFQSEYIVRYYGMFTDDENSSIYIAMEYMGGRSLDAIYKNLLERGGRI 321
Query: 362 SEKEILKIAYQILIQIEELHKFKIVHCDIKCGNVCIDKDGNAKIIDFGLSKD-ISDLSWV 538
SEK + KIA +L + LH+ K++H DIK N+ ++++G K+ DFG+S + ++ L+
Sbjct: 322 SEKVLGKIAEAVLRGLSYLHEKKVIHRDIKPQNILLNENGQVKLCDFGVSGEAVNSLATT 381
Query: 539 FSGTLRYCAPEIIKHENLSFGIDIWAFGMLLVEIVTKNIPFHQIKNN---------RFIF 691
F+GT Y APE I+ + S D+W+ G+ ++E+ P K +I
Sbjct: 382 FTGTSFYMAPERIQGQPYSVTSDVWSLGLTILEVANGKFPCSSEKMAANIAPFELLMWIL 441
Query: 692 EIRSMKKYEVKPIISISKEFLQFIERCLTISSSERPTAMQLL 817
K E + I S F FI+ CL S ERP+ Q++
Sbjct: 442 TFTPELKDEPESNIIWSPSFKSFIDYCLKKDSRERPSPRQMI 483
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 96.7 bits (239), Expect = 7e-20
Identities = 71/244 (29%), Positives = 117/244 (47%), Gaps = 9/244 (3%)
Frame = +2
Query: 116 IGKG-LSRIILLKNNETREQIVLKKPYCEQGE-----IYRDICIQNKLSSSKNLKIYSKL 277
IGKG + +N T++ + +K E+ E I ++I + ++ S K Y
Sbjct: 42 IGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYVTKYYGS- 100
Query: 278 YLFENFICVGMEYIPNESLDSYLKTTDLSEKEILKIAYQILIQIEELHKFKIVHCDIKCG 457
YL + + + MEY+ S L+ L E +I I +IL ++ LH K +H DIK
Sbjct: 101 YLKDTKLWIIMEYLGGGSALDLLEPGPLDETQIATILREILKGLDYLHSEKKIHRDIKAA 160
Query: 458 NVCIDKDGNAKIIDFGLSKDISDLSW---VFSGTLRYCAPEIIKHENLSFGIDIWAFGML 628
NV + + G K+ DFG++ ++D F GT + APE+IK DIW+ G+
Sbjct: 161 NVLLSEHGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGIT 220
Query: 629 LVEIVTKNIPFHQIKNNRFIFEIRSMKKYEVKPIISISKEFLQFIERCLTISSSERPTAM 808
+E+ P ++ + +F I ++ + SK +F+E CL S RPTA
Sbjct: 221 AIELARGEPPHSELHPMKVLFLIPKNNPPTLEG--NYSKPLKEFVEACLNKEPSFRPTAK 278
Query: 809 QLLE 820
+LL+
Sbjct: 279 ELLK 282
>sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (Sterile 20/oxidant
stress-response kinase 1) (Ste20/oxidant stress response
kinase-1) (SOK-1) (Ste20-like kinase)
Length = 426
Score = 96.3 bits (238), Expect = 9e-20
Identities = 72/244 (29%), Positives = 115/244 (47%), Gaps = 9/244 (3%)
Frame = +2
Query: 116 IGKG-LSRIILLKNNETREQIVLKKPYCEQGE-----IYRDICIQNKLSSSKNLKIYSKL 277
IGKG + +N T+E + +K E+ E I ++I + ++ S + +
Sbjct: 26 IGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYITRYFGS- 84
Query: 278 YLFENFICVGMEYIPNESLDSYLKTTDLSEKEILKIAYQILIQIEELHKFKIVHCDIKCG 457
YL + + MEY+ S LK L E I I +IL ++ LH + +H DIK
Sbjct: 85 YLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAA 144
Query: 458 NVCIDKDGNAKIIDFGLSKDISDLSW---VFSGTLRYCAPEIIKHENLSFGIDIWAFGML 628
NV + + G+ K+ DFG++ ++D F GT + APE+IK F DIW+ G+
Sbjct: 145 NVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGIT 204
Query: 629 LVEIVTKNIPFHQIKNNRFIFEIRSMKKYEVKPIISISKEFLQFIERCLTISSSERPTAM 808
+E+ P + R +F I ++ SK F +F+E CL RPTA
Sbjct: 205 AIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEG--QHSKPFKEFVEACLNKDPRFRPTAK 262
Query: 809 QLLE 820
+LL+
Sbjct: 263 ELLK 266
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 95.9 bits (237), Expect = 1e-19
Identities = 71/244 (29%), Positives = 117/244 (47%), Gaps = 9/244 (3%)
Frame = +2
Query: 116 IGKG-LSRIILLKNNETREQIVLKKPYCEQGE-----IYRDICIQNKLSSSKNLKIYSKL 277
IGKG + +N T++ + +K E+ E I ++I + ++ S K Y
Sbjct: 30 IGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYVTKYYGS- 88
Query: 278 YLFENFICVGMEYIPNESLDSYLKTTDLSEKEILKIAYQILIQIEELHKFKIVHCDIKCG 457
YL + + + MEY+ S L+ L E +I I +IL ++ LH K +H DIK
Sbjct: 89 YLKDTKLWIIMEYLGGGSALDLLEPGPLDEIQIATILREILKGLDYLHSEKKIHRDIKAA 148
Query: 458 NVCIDKDGNAKIIDFGLSKDISDLSW---VFSGTLRYCAPEIIKHENLSFGIDIWAFGML 628
NV + + G K+ DFG++ ++D F GT + APE+IK DIW+ G+
Sbjct: 149 NVLLSEHGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGIT 208
Query: 629 LVEIVTKNIPFHQIKNNRFIFEIRSMKKYEVKPIISISKEFLQFIERCLTISSSERPTAM 808
+E+ P ++ + +F I ++ + SK +F+E CL S RPTA
Sbjct: 209 AIELAKGEPPHSELHPMKVLFLIPKNNPPTLEG--NYSKPLKEFVEACLNKEPSFRPTAK 266
Query: 809 QLLE 820
+LL+
Sbjct: 267 ELLK 270
>sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1
Length = 1116
Score = 95.9 bits (237), Expect = 1e-19
Identities = 71/261 (27%), Positives = 129/261 (49%), Gaps = 25/261 (9%)
Frame = +2
Query: 110 KVIGKGL-SRIILLKNNETREQIVLKKPYCEQGEIYRDICIQNKLSSSKNLKI------- 265
++IG G ++ L N T E I +K+ Q R ++ + S N +I
Sbjct: 829 ELIGNGTYGKVFLAMNINTGELIAVKQVEIPQTINGRHDQLRKDIVDSINAEISMIADLD 888
Query: 266 ------YSKLYLFENFICVGMEYIPNESLDSYLKTTDLSEKEILK-IAYQILIQIEELHK 424
Y E I + +EY+ S+ L+ E+++++ ++ Q+L + LH
Sbjct: 889 HLNIVQYLGFEKTETDISIFLEYVSGGSIGRCLRNYGPFEEQLVRFVSRQVLYGLSYLHS 948
Query: 425 FKIVHCDIKCGNVCIDKDGNAKIIDFGLSKDI-----SDLSWVFSGTLRYCAPEII--KH 583
I+H D+K N+ ID DG KI DFG+SK +D + G++ + APE+I H
Sbjct: 949 KGIIHRDLKADNLLIDFDGVCKISDFGISKHSDNVYDNDANLSMQGSIFWMAPEVIHNDH 1008
Query: 584 ENLSFGIDIWAFGMLLVEIVTKNIPFHQIKNNRFIFEIRSMKKYEVKP---IISISKEFL 754
+ S +D+W+ G +++E++ P+ + + +F++ + KK P + +S E +
Sbjct: 1009 QGYSAKVDVWSLGCVVLEMLAGRRPWSTDEAIQAMFKLGTEKKAPPIPSELVSQVSPEAI 1068
Query: 755 QFIERCLTISSSERPTAMQLL 817
QF+ C T+++ RPTA +LL
Sbjct: 1069 QFLNACFTVNADVRPTAEELL 1089
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 95.5 bits (236), Expect = 2e-19
Identities = 72/244 (29%), Positives = 117/244 (47%), Gaps = 9/244 (3%)
Frame = +2
Query: 116 IGKG-LSRIILLKNNETREQIVLKKPYCEQGE-----IYRDICIQNKLSSSKNLKIYSKL 277
IGKG + +N T++ + +K E+ E I ++I + ++ SS K Y
Sbjct: 30 IGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSSYVTKYYGS- 88
Query: 278 YLFENFICVGMEYIPNESLDSYLKTTDLSEKEILKIAYQILIQIEELHKFKIVHCDIKCG 457
YL + + + MEY+ S L+ E +I + +IL ++ LH K +H DIK
Sbjct: 89 YLKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKKIHRDIKAA 148
Query: 458 NVCIDKDGNAKIIDFGLSKDISDLSW---VFSGTLRYCAPEIIKHENLSFGIDIWAFGML 628
NV + + G+ K+ DFG++ ++D F GT + APE+I+ DIW+ G+
Sbjct: 149 NVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGIT 208
Query: 629 LVEIVTKNIPFHQIKNNRFIFEIRSMKKYEVKPIISISKEFLQFIERCLTISSSERPTAM 808
+E+ P + R +F I K I +K F +FI+ CL S RPTA
Sbjct: 209 AIELAKGEPPNSDMHPMRVLFLI--PKNNPPTLIGDFTKSFKEFIDACLNKDPSFRPTAK 266
Query: 809 QLLE 820
+LL+
Sbjct: 267 ELLK 270
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,579,833
Number of Sequences: 369166
Number of extensions: 1911566
Number of successful extensions: 8013
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6345
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6703
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8197207050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)