Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_G11
(848 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 108 1e-23
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 105 2e-22
sp|P27636|CDC15_YEAST Cell division control protein 15 105 2e-22
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 104 4e-22
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 104 4e-22
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 103 6e-22
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 101 2e-21
sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinas... 100 5e-21
sp|P41892|CDC7_SCHPO Cell division control protein 7 100 5e-21
sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinas... 99 1e-20
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 108 bits (271), Expect = 1e-23
Identities = 69/196 (35%), Positives = 109/196 (55%), Gaps = 6/196 (3%)
Frame = +1
Query: 274 KHEELGHGPFGSVYRCSKFGSKDMVVLKKYK-NPNDKDEE-----LKKLMVEFSIYEKLG 435
K E +G G FG VY S +++ +K+ P+ +E +++L E + + L
Sbjct: 70 KGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKNLS 129
Query: 436 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 615
HP I++ G R + I ++++ GS+ S L K G+ EP II ++KQ+L L++LH
Sbjct: 130 -HPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLH 188
Query: 616 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 795
+ I+HRD+KG IL+DN I+L F ++K + ++ + A+S GT W APEV
Sbjct: 189 NNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATV----NGAKSMKGTPYWMAPEV 244
Query: 796 INGEGYGSSADIWSFG 843
I G+ SADIWS G
Sbjct: 245 ILQTGHSFSADIWSVG 260
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 105 bits (261), Expect = 2e-22
Identities = 70/192 (36%), Positives = 100/192 (52%), Gaps = 6/192 (3%)
Frame = +1
Query: 286 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILK 456
LG G FG VY C + + K+ + D E E+ L E + + L H RI++
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNL-QHERIVQ 426
Query: 457 CNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNIL 630
G R + K I ++YM GS++ L GA E ++++QILE + +LHS I+
Sbjct: 427 YYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIV 486
Query: 631 HRDLKGPKILLDNNLDIKLCGFMSAKMFPD-ASSETFRNHVAESNHGTANWTAPEVINGE 807
HRD+KG IL D+ ++KL F ++K S T V GT W +PEVI+GE
Sbjct: 487 HRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVT----GTPYWMSPEVISGE 542
Query: 808 GYGSSADIWSFG 843
GYG AD+WS G
Sbjct: 543 GYGRKADVWSLG 554
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 105 bits (261), Expect = 2e-22
Identities = 66/194 (34%), Positives = 97/194 (50%), Gaps = 1/194 (0%)
Frame = +1
Query: 268 YIKHEELGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPR 447
Y + +G G +G VY+ + +V +K+ ND EEL +M E S+ + L +H
Sbjct: 25 YHLKQVIGRGSYGVVYKAINKHTDQVVAIKEVVYEND--EELNDIMAEISLLKNL-NHNN 81
Query: 448 ILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQ-KEPSIIRFSKQILEALDFLHSQN 624
I+K +GF R YI L+Y ANGSLR + ++ E + Q L L +LH +
Sbjct: 82 IVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEG 141
Query: 625 ILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVING 804
++HRD+K ILL + +KL F T N A + GT NW APE++
Sbjct: 142 VIHRDIKAANILLSADNTVKLADF---------GVSTIVNSSALTLAGTLNWMAPEILGN 192
Query: 805 EGYGSSADIWSFGA 846
G + +DIWS GA
Sbjct: 193 RGASTLSDIWSLGA 206
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 104 bits (259), Expect = 4e-22
Identities = 68/191 (35%), Positives = 99/191 (51%), Gaps = 5/191 (2%)
Frame = +1
Query: 286 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILK 456
LG G FG VY C + + K+ + D E E+ L E + + L H RI++
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNL-QHERIVQ 426
Query: 457 CNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNIL 630
G R + K I ++YM GS++ L GA E ++++QILE + +LHS I+
Sbjct: 427 YYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIV 486
Query: 631 HRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEG 810
HRD+KG IL D+ ++KL F ++K + S GT W +PEVI+GEG
Sbjct: 487 HRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGIRSVTGTPYWMSPEVISGEG 543
Query: 811 YGSSADIWSFG 843
YG AD+WS G
Sbjct: 544 YGRKADVWSLG 554
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 104 bits (259), Expect = 4e-22
Identities = 65/196 (33%), Positives = 104/196 (53%), Gaps = 6/196 (3%)
Frame = +1
Query: 274 KHEELGHGPFGSVYRCSKFGSKDMVVLKK------YKNPNDKDEELKKLMVEFSIYEKLG 435
K + +G G FG+VY S +++ +K+ + + +++L E + + L
Sbjct: 71 KGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLS 130
Query: 436 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 615
HP I++ G R I L+++ GS+ S L K G E + +++Q+L L++LH
Sbjct: 131 -HPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLH 189
Query: 616 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 795
+ I+HRD+KG IL+DN IKL F ++K + ++ T A+S GT W APEV
Sbjct: 190 NHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMT----GAKSMKGTPYWMAPEV 245
Query: 796 INGEGYGSSADIWSFG 843
I G+ SADIWS G
Sbjct: 246 ILQTGHSFSADIWSVG 261
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 103 bits (257), Expect = 6e-22
Identities = 67/196 (34%), Positives = 107/196 (54%), Gaps = 6/196 (3%)
Frame = +1
Query: 274 KHEELGHGPFGSVYRCSKFGSKDMVVLKKY---KNPNDKDE---ELKKLMVEFSIYEKLG 435
K + +G G FG+VY S +++ +K+ N K++ +++L E + + L
Sbjct: 70 KGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLLKNLS 129
Query: 436 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 615
HP I++ G R + I L+++ GS+ S L K GA E + ++ Q+L L++LH
Sbjct: 130 -HPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLH 188
Query: 616 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 795
+ I+HRD+KG IL+DN IKL F ++K + ++ + A+S GT W APEV
Sbjct: 189 NHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATIS----GAKSMKGTPYWMAPEV 244
Query: 796 INGEGYGSSADIWSFG 843
I G+ SADIWS G
Sbjct: 245 ILQTGHSFSADIWSVG 260
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 101 bits (252), Expect = 2e-21
Identities = 67/189 (35%), Positives = 98/189 (51%), Gaps = 5/189 (2%)
Frame = +1
Query: 286 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILK 456
LG G FG VY C + + +K+ + D E E+ L E + + H RI++
Sbjct: 361 LGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNFL-HERIVQ 419
Query: 457 CNGFFRSV--KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNIL 630
G R K I ++YM GS++ L GA E ++++QILE + +LHS IL
Sbjct: 420 YYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYLHSNMIL 479
Query: 631 HRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEG 810
HRD+KG IL D+ ++KL F ++K + +S GT W +PEVI+G+G
Sbjct: 480 HRDIKGANILRDSTGNVKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPEVISGQG 536
Query: 811 YGSSADIWS 837
YG ADIWS
Sbjct: 537 YGRKADIWS 545
>sp|Q13233|M3K1_HUMAN Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1495
Score = 100 bits (249), Expect = 5e-21
Identities = 66/234 (28%), Positives = 117/234 (50%), Gaps = 5/234 (2%)
Frame = +1
Query: 157 QDENKEKQVKWDEDYEQLISGGKSISLFYLPTPDFDDYIKHEELGHGPFGSVYRCSKFGS 336
Q EN E + +D + + G P + +++K +++G G F S Y+ G+
Sbjct: 1192 QVENGEDIIIIQQDTPETLPGHTKAKQ---PYREDTEWLKGQQIGLGAFSSCYQAQDVGT 1248
Query: 337 KDMVVLKKY----KNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLD 504
++ +K+ ++++E ++ L E + L +HP I++ G + ++
Sbjct: 1249 GTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHL-NHPNIIRMLGATCEKSNYNLFIE 1307
Query: 505 YMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDN-NLDI 681
+MA GS+ L K GA KE +I +++Q+L L +LH I+HRD+KG +L+D+ +
Sbjct: 1308 WMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRL 1367
Query: 682 KLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFG 843
++ F +A AS T GT + APEV+ G+ YG S D+WS G
Sbjct: 1368 RIADFGAAARL--ASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVG 1419
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 100 bits (249), Expect = 5e-21
Identities = 65/186 (34%), Positives = 96/186 (51%)
Frame = +1
Query: 286 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNG 465
LG G FG+VYR + + V +KK K +L + +E + + L DHP I+K G
Sbjct: 15 LGKGAFGAVYRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLKNL-DHPNIVKYRG 73
Query: 466 FFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLK 645
+++ + I L+Y NGSLRS G E + ++ Q+L+ L +LH+Q ++HRD+K
Sbjct: 74 SYQTNDSLCIILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLHNQGVIHRDIK 133
Query: 646 GPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSA 825
G IL + IKL F A +H S G+ W APEVI G +++
Sbjct: 134 GANILTTKDGTIKLADFGVA-----TKINALEDH---SVVGSPYWMAPEVIELVGATTAS 185
Query: 826 DIWSFG 843
DIWS G
Sbjct: 186 DIWSVG 191
>sp|P53349|M3K1_MOUSE Mitogen-activated protein kinase kinase kinase 1 (MAPK/ERK kinase
kinase 1) (MEK kinase 1) (MEKK 1)
Length = 1493
Score = 99.4 bits (246), Expect = 1e-20
Identities = 66/234 (28%), Positives = 117/234 (50%), Gaps = 5/234 (2%)
Frame = +1
Query: 157 QDENKEKQVKWDEDYEQLISGGKSISLFYLPTPDFDDYIKHEELGHGPFGSVYRCSKFGS 336
Q EN E + +D + + G P + +++K +++G G F S Y+ G+
Sbjct: 1190 QVENGEDIIIIQQDTPETLPGHTKAKQ---PYREDAEWLKGQQIGLGAFSSCYQAQDVGT 1246
Query: 337 KDMVVLKKY----KNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLD 504
++ +K+ ++++E ++ L E + L +HP I++ G + ++
Sbjct: 1247 GTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHL-NHPNIIRMLGATCEKSNYNLFIE 1305
Query: 505 YMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDN-NLDI 681
+MA GS+ L K GA KE +I +++Q+L L +LH I+HRD+KG +L+D+ +
Sbjct: 1306 WMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRL 1365
Query: 682 KLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFG 843
++ F +A AS T GT + APEV+ G+ YG S D+WS G
Sbjct: 1366 RIADFGAAARL--ASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVG 1417
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,888,202
Number of Sequences: 369166
Number of extensions: 2043595
Number of successful extensions: 9049
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7040
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7468
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8341863645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)