Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_F12
(956 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9H3E2|SNX25_HUMAN Sorting nexin-25 65 4e-10
sp|P50102|UBP8_YEAST Ubiquitin carboxyl-terminal hydrolase ... 33 1.5
sp|Q12496|YOJ8_YEAST Hypothetical 118.4 kDa protein in WRS1... 30 7.7
sp|Q8XHL1|TILS_CLOPE tRNA(Ile)-lysidine synthase (tRNA(Ile)... 30 7.7
>sp|Q9H3E2|SNX25_HUMAN Sorting nexin-25
Length = 538
Score = 64.7 bits (156), Expect = 4e-10
Identities = 64/265 (24%), Positives = 111/265 (41%), Gaps = 2/265 (0%)
Frame = +2
Query: 62 FIMVSLVSALDQYNSKEDNGWLVKHTLKDFQALKRELNAINNSIEKISLPKVPQRLFMRR 241
F+MVSL + E W V L +FQ L R+L+ S++K+ LP + + F +
Sbjct: 229 FVMVSL----QEVGGVETKNWTVPRRLSEFQNLHRKLSECVPSLKKVQLPSLSKLPF--K 282
Query: 242 KYTDNDLTHIKDSLNKFIQFIVKSDILAKSEPVFKFFCNSVESGQMPNEDTL-MGPLSNF 418
+ K+ LNKF+Q ++ + L +SE ++ F P+ D L + +
Sbjct: 283 SIDQKFMEKSKNQLNKFLQNLLSDERLCQSEALYAFL--------SPSPDYLKVIDVQGK 334
Query: 419 ANTLSDASAKVMLMAKIPSKFVQKVLAPRKHSGSATSLDSESTDSKSDKRNAFRRXXXXX 598
N+ S +S + ++P F H T DS+ +D D
Sbjct: 335 KNSFSLSS----FLERLPRDFF-------SHQEEETEEDSDLSDYGDD------------ 371
Query: 599 XXXXXLKHKLRKFSHSKD-ISMSAFELLEELIQLRKSLTLTRRSMITFVQSFFGKNLTQI 775
KD ++ F L+ E+ +LR RR++I VQ FG+ + +
Sbjct: 372 ------------VDGRKDALAEPCFMLIGEIFELRGMFKWVRRTLIALVQVTFGRTINKQ 419
Query: 776 LTEKVACIKSESTVFVCLQKYRDSF 850
+ + V+ I SE + + +RD+F
Sbjct: 420 IRDTVSWIFSEQMLVYYINIFRDAF 444
>sp|P50102|UBP8_YEAST Ubiquitin carboxyl-terminal hydrolase 8 (Ubiquitin thiolesterase 8)
(Ubiquitin-specific processing protease 8)
(Deubiquitinating enzyme 8)
Length = 471
Score = 32.7 bits (73), Expect = 1.5
Identities = 21/75 (28%), Positives = 37/75 (49%)
Frame = +2
Query: 77 LVSALDQYNSKEDNGWLVKHTLKDFQALKRELNAINNSIEKISLPKVPQRLFMRRKYTDN 256
L LD ++ KE LKDF E N+ ++I+++ + K+P L ++ K ++
Sbjct: 316 LYECLDSFHKKEQ--------LKDFNYHCGECNSTQDAIKQLGIHKLPSVLVLQLKRFEH 367
Query: 257 DLTHIKDSLNKFIQF 301
L L+ FI+F
Sbjct: 368 LLNGSNRKLDDFIEF 382
>sp|Q12496|YOJ8_YEAST Hypothetical 118.4 kDa protein in WRS1-PKH2 intergenic region
Length = 1037
Score = 30.4 bits (67), Expect = 7.7
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = +2
Query: 206 LPKVPQRLFMRRKYTDNDLTHIKDSLNKFIQFIVKSDILAKSE 334
LP++P + +R + DN L+HI S +K + V+ L +S+
Sbjct: 236 LPEIPSNIPKKRPFLDNKLSHIPQSRDKVTESTVEFPELDESQ 278
>sp|Q8XHL1|TILS_CLOPE tRNA(Ile)-lysidine synthase (tRNA(Ile)-lysidine synthetase)
(tRNA(Ile)-2-lysyl-cytidine synthase)
Length = 469
Score = 30.4 bits (67), Expect = 7.7
Identities = 27/91 (29%), Positives = 48/91 (52%), Gaps = 8/91 (8%)
Frame = +2
Query: 86 ALDQYNSKEDNGWLVKHTLKDFQALK-----RELNAINNSIEKISLPKVPQRLFMRRKYT 250
AL + +SK +N + +KH ++D ALK +++N N I + +L +K
Sbjct: 273 ALFEVSSKHNN-FELKH-IQDIIALKDKGTGKQINITNGVIALNEYGDIRIKLVDSKKAK 330
Query: 251 DN---DLTHIKDSLNKFIQFIVKSDILAKSE 334
+N +L +IKD L+K + +++ DIL E
Sbjct: 331 ENKVLNLENIKDELDKNQKVVIEDDILGNYE 361
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,661,814
Number of Sequences: 369166
Number of extensions: 1664363
Number of successful extensions: 4292
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 4121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4287
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 9904824765
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)