Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_E10
(477 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP... 119 4e-27
sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP... 107 1e-23
sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cy... 91 2e-18
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 90 3e-18
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 87 2e-17
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 87 2e-17
sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cy... 87 2e-17
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 86 3e-17
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 86 4e-17
sp|P21642|PPCKM_CHICK Phosphoenolpyruvate carboxykinase, mi... 85 8e-17
>sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 619
Score = 119 bits (298), Expect = 4e-27
Identities = 58/98 (59%), Positives = 71/98 (72%)
Frame = +1
Query: 1 HMPICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTK 180
H+PI KGD+H LP VQ F+A+ A + +PR +YICDG + EA+EII KL ERG L L
Sbjct: 6 HVPIHKGDFHLLPPKVQRFVAEKAELMRPRGIYICDGSQHEADEIIDKLIERGMLSPLKA 65
Query: 181 LENCYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
EN YICRTDP DVARVESKT++ T DK+ TV H +G
Sbjct: 66 YENNYICRTDPKDVARVESKTWMVTPDKYQTVCHTPDG 103
>sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 643
Score = 107 bits (267), Expect = 1e-23
Identities = 51/98 (52%), Positives = 71/98 (72%)
Frame = +1
Query: 1 HMPICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTK 180
H+P+ KGD+ LPK VQ F+A+ A + KP A++ICDG ++EA+E+I + ERG L L
Sbjct: 27 HIPVIKGDFVSLPKHVQRFVAEKAELMKPSAIFICDGSQNEADELIARCVERGVLVPLKA 86
Query: 181 LENCYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
+N Y+CRTDP DVARVESKT++ T +K+ +V H EG
Sbjct: 87 YKNNYLCRTDPRDVARVESKTWMITPEKYDSVCHTPEG 124
>sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 90.5 bits (223), Expect = 2e-18
Identities = 40/95 (42%), Positives = 63/95 (66%)
Frame = +1
Query: 10 ICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKLEN 189
I +G LP+AV+ F+ A +C+P ++ICDG E+E ++++ ++E G + L K +N
Sbjct: 15 IVRGSLDSLPQAVRSFVESSAKLCRPDQVHICDGSEEENRQLLSHMEEEGVIKRLKKYDN 74
Query: 190 CYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
C++ TDP DVAR+ESKT I T+++ TVP K G
Sbjct: 75 CWLALTDPRDVARIESKTVIITREQRDTVPIPKNG 109
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 89.7 bits (221), Expect = 3e-18
Identities = 43/95 (45%), Positives = 60/95 (63%)
Frame = +1
Query: 10 ICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKLEN 189
+ GD LP V+ F+A+ A +C+P ++ICDG E E I+ L+E+G + L K +N
Sbjct: 32 VLSGDMSQLPAGVRDFVARSAHLCQPEGIHICDGTEAENTAILALLEEQGLIRKLPKYKN 91
Query: 190 CYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
C++ RTDP DVARVESKT I T + TVP + G
Sbjct: 92 CWLARTDPKDVARVESKTVIVTPSQRDTVPLLAGG 126
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 87.4 bits (215), Expect = 2e-17
Identities = 39/95 (41%), Positives = 62/95 (65%)
Frame = +1
Query: 10 ICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKLEN 189
+ +G LP+AV+ F+ A +C+P ++ICDG E+E +++ ++E G + L K +N
Sbjct: 15 VIQGSLDSLPQAVRKFVEGNAQLCQPEYIHICDGSEEEYGQLLAHMQEEGVIRKLKKYDN 74
Query: 190 CYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
C++ TDP DVAR+ESKT I T+++ TVP K G
Sbjct: 75 CWLALTDPRDVARIESKTVIITQEQRDTVPIPKTG 109
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 87.0 bits (214), Expect = 2e-17
Identities = 39/96 (40%), Positives = 61/96 (63%)
Frame = +1
Query: 10 ICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKLEN 189
+ +G LP+ V+ F+ A +C+P ++ICDG E+E ++ ++E G + L K +N
Sbjct: 15 VIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDN 74
Query: 190 CYICRTDPDDVARVESKTFISTKDKHMTVPHVKEGQ 297
C++ TDP DVAR+ESKT I T+++ TVP K GQ
Sbjct: 75 CWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQ 110
>sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 87.0 bits (214), Expect = 2e-17
Identities = 40/95 (42%), Positives = 63/95 (66%)
Frame = +1
Query: 10 ICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKLEN 189
+ +GD LP V+ FI A +C+P +++ICDG E+E +I+ + E+G + L+K EN
Sbjct: 15 VIQGDLESLPPQVREFIESNAKLCQPESIHICDGSEEENKKILDIMVEQGMIKKLSKYEN 74
Query: 190 CYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
C++ T+P DVAR+ESKT I T+++ T+P K G
Sbjct: 75 CWLALTNPRDVARIESKTVIITQEQRDTIPIPKTG 109
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 86.3 bits (212), Expect = 3e-17
Identities = 40/95 (42%), Positives = 62/95 (65%)
Frame = +1
Query: 10 ICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKLEN 189
+ +G LP+AV+ F+ A +C+P ++ICDG E+E ++ +++E G L L K +N
Sbjct: 15 VVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDN 74
Query: 190 CYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
C++ TDP DVAR+ESKT I T+++ TVP K G
Sbjct: 75 CWLALTDPRDVARIESKTVIVTQEQRDTVPIPKTG 109
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 85.9 bits (211), Expect = 4e-17
Identities = 41/97 (42%), Positives = 60/97 (61%)
Frame = +1
Query: 4 MPICKGDYHYLPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKL 183
+PI G+ L V+ F+ + +C+P ++ICDG E E +I L E GT+ L K
Sbjct: 38 IPISYGNVDLLTTGVRAFVEEGIALCQPDQVHICDGSEQENKVLIKSLLEAGTIVPLPKY 97
Query: 184 ENCYICRTDPDDVARVESKTFISTKDKHMTVPHVKEG 294
+NC++ RT+P DVARVES+TFI T+ + T+P EG
Sbjct: 98 DNCWLARTNPADVARVESRTFICTERREETIPTPVEG 134
>sp|P21642|PPCKM_CHICK Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 85.1 bits (209), Expect = 8e-17
Identities = 37/82 (45%), Positives = 55/82 (67%)
Frame = +1
Query: 34 LPKAVQGFIAKWALICKPRALYICDGGEDEANEIITKLKERGTLHALTKLENCYICRTDP 213
LP A + F+ + +C+PR + +CDG E+E E++ L++ G LH L K +NC++ RTDP
Sbjct: 41 LPAAARDFVEEAVRLCRpreVLLCDGSEEEGKELLRGLQDDGVLHPLPKYDNCWLARTDP 100
Query: 214 DDVARVESKTFISTKDKHMTVP 279
DVARVESKT + T ++ VP
Sbjct: 101 RDVARVESKTVLVTPEQSDAVP 122
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,612,426
Number of Sequences: 369166
Number of extensions: 1076260
Number of successful extensions: 2442
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2423
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2832714465
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)