Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_C16
(666 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P92177|1433E_DROME 14-3-3 protein epsilon (Suppressor of... 249 6e-66
sp|P62262|1433E_SHEEP 14-3-3 protein epsilon (14-3-3E) (Pro... 246 4e-65
sp|P54632|1433_DICDI 14-3-3-like protein 240 2e-63
sp|P93212|1437_LYCES 14-3-3 protein 7 239 5e-63
sp|Q43470|1433B_HORVU 14-3-3-like protein B (14-3-3B) 237 2e-62
sp|P52908|1433_CHLRE 14-3-3-like protein 236 3e-62
sp|P93208|14332_LYCES 14-3-3 protein 2 236 3e-62
sp|Q96453|1433D_SOYBN 14-3-3-like protein D (SGF14D) 236 3e-62
sp|Q9C5W6|143C_ARATH 14-3-3-like protein GF14 iota (General... 236 4e-62
sp|P93210|14335_LYCES 14-3-3 protein 5 236 5e-62
>sp|P92177|1433E_DROME 14-3-3 protein epsilon (Suppressor of Ras1 3-9)
Length = 262
Score = 249 bits (635), Expect = 6e-66
Identities = 126/187 (67%), Positives = 152/187 (81%), Gaps = 4/187 (2%)
Frame = +1
Query: 7 IMSSIEQKAEQKDPS-RLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I++SIEQK E K +L +IK YR QVEKEL+++C+DI+++L+ H++ AT+GE+KVF+
Sbjct: 62 IITSIEQKEENKGAEEKLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKVFY 121
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
KMKGDY+RYL+EF ++RK AAENSL AYK A+ IA +DL PTHPIRLGLALNFSVFY
Sbjct: 122 YKMKGDYHRYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSVFY 181
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSD---ADQD 534
YEILNS DRAC+LAK AFDDAI ELD L+EESYKDSTLIMQLLRDNLTLWTSD + D
Sbjct: 182 YEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQAEEVD 241
Query: 535 DKVGDEE 555
GD E
Sbjct: 242 PNAGDGE 248
>sp|P62262|1433E_SHEEP 14-3-3 protein epsilon (14-3-3E) (Protein kinase C inhibitor
protein 1) (KCIP-1)
sp|P62260|1433E_RAT 14-3-3 protein epsilon (14-3-3E) (Mitochondrial import stimulation
factor L subunit) (MSF L)
sp|P62259|1433E_MOUSE 14-3-3 protein epsilon (14-3-3E)
sp|P62258|1433E_HUMAN 14-3-3 protein epsilon (14-3-3E)
sp|P62261|1433E_BOVIN 14-3-3 protein epsilon (14-3-3E)
Length = 255
Score = 246 bits (628), Expect = 4e-65
Identities = 126/185 (68%), Positives = 147/185 (79%), Gaps = 1/185 (0%)
Frame = +1
Query: 7 IMSSIEQKAEQKD-PSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E K +L +I+ YR VE ELK +C DI+ +LD H++ AA GE+KVF+
Sbjct: 62 IISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFY 121
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
KMKGDY+RYL+EF N+RK AAENSL AYK A+ IA ++L PTHPIRLGLALNFSVFY
Sbjct: 122 YKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFY 181
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQDDKV 543
YEILNS DRAC+LAK AFDDAI ELD L+EESYKDSTLIMQLLRDNLTLWTSD D
Sbjct: 182 YEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGD--- 238
Query: 544 GDEEN 558
G+E+N
Sbjct: 239 GEEQN 243
>sp|P54632|1433_DICDI 14-3-3-like protein
Length = 252
Score = 240 bits (613), Expect = 2e-63
Identities = 121/188 (64%), Positives = 151/188 (80%), Gaps = 3/188 (1%)
Frame = +1
Query: 7 IMSSIEQKAEQK-DPSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E K + + + IK Y+ +VEKEL ++C DI+ +L+ H++V++ +GE+KVF+
Sbjct: 61 IISSIEQKEESKGNENHVKKIKEYKCKVEKELTDICNDILEVLESHLIVSSASGESKVFY 120
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
KMKGDY+RYL+EF N RKT+AE+SL AYK A+ IA ++L PTHPIRLGLALNFSVFY
Sbjct: 121 YKMKGDYFRYLAEFATGNPRKTSAESSLIAYKAASDIAVTELPPTHPIRLGLALNFSVFY 180
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQDDK- 540
YEILNS DRAC LAK AFDDAI ELD L+E+SYKDSTLIMQLLRDNLTLWTSD +K
Sbjct: 181 YEILNSPDRACNLAKTAFDDAIAELDTLSEDSYKDSTLIMQLLRDNLTLWTSDVHNMEKN 240
Query: 541 -VGDEENN 561
GD++ N
Sbjct: 241 QDGDDDQN 248
>sp|P93212|1437_LYCES 14-3-3 protein 7
Length = 252
Score = 239 bits (610), Expect = 5e-63
Identities = 120/185 (64%), Positives = 150/185 (81%), Gaps = 3/185 (1%)
Frame = +1
Query: 7 IMSSIEQKAEQKDPSR-LPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E K + + IK YR +VE EL ++C+DI+S++D+H++ ++T GE+ VF+
Sbjct: 63 ILSSIEQKEESKGHEQNVKRIKTYRQRVEDELTKICSDILSVIDEHLVPSSTTGESTVFY 122
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
KMKGDYYRYL+EF ++RK A+E SL AY+ ATA A SDL PTHPIRLGLALNFSVFY
Sbjct: 123 YKMKGDYYRYLAEFKAGDDRKEASEQSLKAYEAATATASSDLAPTHPIRLGLALNFSVFY 182
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQ--DD 537
YEILNS +RAC LAK+AFD+AI ELD+L+EESYKDSTLIMQLLRDNLTLWTSD ++ +
Sbjct: 183 YEILNSPERACHLAKQAFDEAIAELDSLSEESYKDSTLIMQLLRDNLTLWTSDLEEGGEH 242
Query: 538 KVGDE 552
GDE
Sbjct: 243 SKGDE 247
>sp|Q43470|1433B_HORVU 14-3-3-like protein B (14-3-3B)
Length = 262
Score = 237 bits (604), Expect = 2e-62
Identities = 119/177 (67%), Positives = 145/177 (81%), Gaps = 1/177 (0%)
Frame = +1
Query: 7 IMSSIEQKAEQK-DPSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E + + R+ LIK YR ++E EL ++C I+ LLD H++ ++T E+KVF+
Sbjct: 69 IISSIEQKEESRGNEDRVTLIKDYRGKIEVELTKICDGILKLLDSHLVPSSTAPESKVFY 128
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
LKMKGDYYRYL+EF ERK AAEN++ AYK A IA ++L PTHPIRLGLALNFSVFY
Sbjct: 129 LKMKGDYYRYLAEFKSGTERKDAAENTMVAYKAAQEIALAELPPTHPIRLGLALNFSVFY 188
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQD 534
YEILNS DRAC LAK+AFD+AI+ELD+L+EESYKDSTLIMQLLRDNLTLWTSD +D
Sbjct: 189 YEILNSPDRACDLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDISED 245
>sp|P52908|1433_CHLRE 14-3-3-like protein
Length = 259
Score = 236 bits (603), Expect = 3e-62
Identities = 122/183 (66%), Positives = 146/183 (79%), Gaps = 1/183 (0%)
Frame = +1
Query: 7 IMSSIEQKAEQK-DPSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E K + + I+ YR+ VE+EL ++CA I+ LLDDH++ A+ GE+KVF+
Sbjct: 65 IISSIEQKEEAKGNEEHVQRIRKYRTVVEEELSKICASILQLLDDHLIPTASTGESKVFY 124
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
LKMKGDY+RYL+EF +RK AAE++L AYK A IA DL PTHPIRLGLALNFSVFY
Sbjct: 125 LKMKGDYHRYLAEFKTGADRKEAAEHTLLAYKAAQDIALVDLPPTHPIRLGLALNFSVFY 184
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQDDKV 543
YEILNS +RAC LAK+AFD+AI ELD+L EESYKDSTLIMQLLRDNLTLWTSD QD
Sbjct: 185 YEILNSPERACHLAKQAFDEAIAELDSLGEESYKDSTLIMQLLRDNLTLWTSDM-QDPAA 243
Query: 544 GDE 552
GD+
Sbjct: 244 GDD 246
>sp|P93208|14332_LYCES 14-3-3 protein 2
Length = 254
Score = 236 bits (603), Expect = 3e-62
Identities = 119/188 (63%), Positives = 150/188 (79%), Gaps = 3/188 (1%)
Frame = +1
Query: 7 IMSSIEQKAEQK-DPSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E + + + IK YRS++E EL ++C I+ LLD +++ +A+NG++KVF+
Sbjct: 63 IISSIEQKEESRGNEEHVKCIKEYRSKIESELSDICDGILKLLDSNLIPSASNGDSKVFY 122
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
LKMKGDY+RYL+EF ERK AAE++L+AYK A IA ++L PTHPIRLGLALNFSVFY
Sbjct: 123 LKMKGDYHRYLAEFKTGAERKEAAESTLSAYKAAQDIANTELAPTHPIRLGLALNFSVFY 182
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQD--D 537
YEILNS DRAC LAK+AFD+AI ELD L EESYKDSTLIMQLLRDNLTLWTSD D D
Sbjct: 183 YEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQDDGAD 242
Query: 538 KVGDEENN 561
++ + +N+
Sbjct: 243 EIKETKND 250
>sp|Q96453|1433D_SOYBN 14-3-3-like protein D (SGF14D)
Length = 261
Score = 236 bits (603), Expect = 3e-62
Identities = 118/185 (63%), Positives = 145/185 (78%), Gaps = 1/185 (0%)
Frame = +1
Query: 7 IMSSIEQKAEQK-DPSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E K + IK YR +VE EL +C D++ ++D+H++ +A GE+ VF+
Sbjct: 65 ILSSIEQKEETKGNELNAKRIKEYRQKVELELSNICNDVMRVIDEHLIPSAAAGESTVFY 124
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
KMKGDYYRYL+EF NE+K AA+ S+ AY+ ATA AE+DL PTHPIRLGLALNFSVFY
Sbjct: 125 YKMKGDYYRYLAEFKSGNEKKEAADQSMKAYESATAAAEADLPPTHPIRLGLALNFSVFY 184
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQDDKV 543
YEILNS +RAC LAK+AFD+AI+ELD LNEESYKDSTLIMQLLRDNLTLWTSD +D +
Sbjct: 185 YEILNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDGED 244
Query: 544 GDEEN 558
+ N
Sbjct: 245 AQKVN 249
>sp|Q9C5W6|143C_ARATH 14-3-3-like protein GF14 iota (General regulatory factor 12)
Length = 268
Score = 236 bits (602), Expect = 4e-62
Identities = 120/185 (64%), Positives = 144/185 (77%), Gaps = 1/185 (0%)
Frame = +1
Query: 7 IMSSIEQKAEQK-DPSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
IMSSIEQK E K + S + IK YR +VE EL +C DI++++D H++ AT+GEA VF+
Sbjct: 68 IMSSIEQKEESKGNESNVKQIKGYRQKVEDELANICQDILTIIDQHLIPHATSGEATVFY 127
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
KMKGDYYRYL+EF + ERK AAE SL Y+ AT A ++L THPIRLGLALNFSVFY
Sbjct: 128 YKMKGDYYRYLAEFKTEQERKEAAEQSLKGYEAATQAASTELPSTHPIRLGLALNFSVFY 187
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQDDKV 543
YEI+NS +RAC LAK+AFD+AI ELD L+EESYKDSTLIMQLLRDNLTLWTSD +D
Sbjct: 188 YEIMNSPERACHLAKQAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDLPED--- 244
Query: 544 GDEEN 558
G E+N
Sbjct: 245 GGEDN 249
>sp|P93210|14335_LYCES 14-3-3 protein 5
Length = 255
Score = 236 bits (601), Expect = 5e-62
Identities = 119/177 (67%), Positives = 138/177 (77%), Gaps = 1/177 (0%)
Frame = +1
Query: 7 IMSSIEQKAEQK-DPSRLPLIKAYRSQVEKELKEVCADIISLLDDHMLVAATNGEAKVFF 183
I+SSIEQK E + + + IK YRSQ+E EL +C I+ LLD ++ +A G++KVF+
Sbjct: 65 IISSIEQKEESRGNEDHVASIKKYRSQIENELTSICNGILKLLDSKLIGSAATGDSKVFY 124
Query: 184 LKMKGDYYRYLSEFTVDNERKTAAENSLTAYKEATAIAESDLQPTHPIRLGLALNFSVFY 363
LKMKGDYYRYL+EF ERK AAEN+L+AYK A IA +L PTHPIRLGLALNFSVFY
Sbjct: 125 LKMKGDYYRYLAEFKTGTERKEAAENTLSAYKSAQDIANGELAPTHPIRLGLALNFSVFY 184
Query: 364 YEILNSADRACQLAKKAFDDAITELDNLNEESYKDSTLIMQLLRDNLTLWTSDADQD 534
YEILNS DRAC LAK+AFD+AI ELD L EESYKDSTLIMQLLRDNLTLWTSD D
Sbjct: 185 YEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQDD 241
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,679,831
Number of Sequences: 369166
Number of extensions: 1011337
Number of successful extensions: 2924
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2892
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5608903050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)