Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_B08
(724 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 75 2e-13
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 72 2e-12
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 61 3e-09
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 57 7e-08
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 55 2e-07
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 38 0.031
sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protei... 34 0.45
sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62) 33 1.0
sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase... 31 3.8
sp|P18745|ZO22_XENLA Oocyte zinc finger protein XLCOF22 31 3.8
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 75.1 bits (183), Expect = 2e-13
Identities = 47/179 (26%), Positives = 81/179 (45%), Gaps = 3/179 (1%)
Frame = +3
Query: 63 KTKQQQCTNDINQYYSLVVNIIVEFLNPSL-CSELGLCNSTIKQVIMRQPLVQINRSKKT 239
K+ ++C ++ Y S +++I++E ++P L CS L LC+ T + V + + K
Sbjct: 351 KSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALT----VHVTQPKDG 406
Query: 240 SNCRLCCKFYNYL-QTISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFN 416
C +C K YL + + S+ +L L C+ + + +C + + V+
Sbjct: 407 GFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEIL 466
Query: 417 KSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
F C C K LG C WGP +WC + + A C AV+HC+ +VW
Sbjct: 467 VEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVW 523
Score = 37.7 bits (86), Expect = 0.031
Identities = 18/38 (47%), Positives = 20/38 (52%)
Frame = +3
Query: 483 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVWRQ 596
LG CT G WC + K A C GAVKHC VW +
Sbjct: 20 LGLKECTRGSAVWCQNVKTASDC--GAVKHCLQTVWNK 55
Score = 30.8 bits (68), Expect = 3.8
Identities = 28/152 (18%), Positives = 58/152 (38%), Gaps = 21/152 (13%)
Frame = +3
Query: 81 CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNS--------------TIKQVIMRQPLVQ 218
C N I+QY + + +++ +C+ +G C+ K VI LV+
Sbjct: 241 CKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVE 300
Query: 219 -INRSKKTSNCRLCCKFYNYL-----QTISSKSSHSNLLFGLVSFCNQISAHERLECLKQ 380
I + + + + C+ +L + I + + +L C+++ EC +
Sbjct: 301 PIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEV 360
Query: 381 FRNFNAVIAAFNKSKTINEF-CEMTKMCSHQR 473
+ + I + + E C M +CS R
Sbjct: 361 VDTYGSSILSILLEEVSPELVCSMLHLCSGTR 392
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 72.0 bits (175), Expect = 2e-12
Identities = 48/188 (25%), Positives = 82/188 (43%), Gaps = 4/188 (2%)
Frame = +3
Query: 39 INEICPHFKTK-QQQCTNDINQYYSLVVNIIVEFLNPSL-CSELGLCNSTIKQVIMRQPL 212
++++C T +QC ++ Y +++I+++ +P L CS L LC+S +
Sbjct: 343 LDKVCSKLPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRG----LPAAT 398
Query: 213 VQINRSKKTSNCRLCCKFYNYL-QTISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRN 389
V++ K C +C K YL + + S+ +L L C+ + R +C +
Sbjct: 399 VRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTE 458
Query: 390 FNAVIAAFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAV 566
+ V+ F C C K LG C WGP +WC + + A C AV
Sbjct: 459 YEPVLIEILVEVMDPSFVCLKIGACPAAHKPLLGAEKCVWGPSYWCQNMESAALC--NAV 516
Query: 567 KHCEMNVW 590
+HC +VW
Sbjct: 517 EHCRRHVW 524
Score = 36.6 bits (83), Expect = 0.070
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +3
Query: 483 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
LG CT G WC + K A C GAV+HC VW
Sbjct: 20 LGLRECTRGSAVWCQNVKTAADC--GAVQHCLQTVW 53
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 61.2 bits (147), Expect = 3e-09
Identities = 42/178 (23%), Positives = 77/178 (43%), Gaps = 5/178 (2%)
Frame = +3
Query: 72 QQQCTNDINQYYSLVVNIIVEFLNP-SLCSELGLC--NSTIKQVIMRQPLVQINRSKKTS 242
+ QC + I Y ++++++E NP ++C L C N +Q ++ +P
Sbjct: 350 KDQCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGGF------- 402
Query: 243 NCRLCCKFYNYLQTISSKSSHSNLLFGLVS-FCNQISAHERLECLKQFRNFNAVIAAFNK 419
C +C Y K++ + + L+ C+ + +C++ + V+
Sbjct: 403 -CDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLA 461
Query: 420 SKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
F C +C +K LG++ C WGPG+WC + + A C AV HC +VW
Sbjct: 462 EMMDPTFVCTKLGVCGAAKKPLLGEDACVWGPGYWCKNMETAAQC--NAVDHCRRHVW 517
Score = 40.8 bits (94), Expect = 0.004
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = +3
Query: 483 LGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
L + C GP WC S + A CG AVKHC+ NVW
Sbjct: 21 LWQKDCAKGPEVWCQSLRTASQCG--AVKHCQQNVW 54
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 56.6 bits (135), Expect = 7e-08
Identities = 43/201 (21%), Positives = 76/201 (37%), Gaps = 30/201 (14%)
Frame = +3
Query: 78 QCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNS-------------TIKQVIMRQPL- 212
+C + + +++I + +NPS LC +GLC + I ++++P
Sbjct: 358 KCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTP 417
Query: 213 -------------VQINRSKKTSNCRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQIS 350
+ K C +C K YL+ + S+ +L L C+ +
Sbjct: 418 PKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLP 477
Query: 351 AHERLECLKQFRNFNAVIAAFNKSKTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCV 527
+ +C + ++ F C +C K LG C WGP +WC
Sbjct: 478 DPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQ 537
Query: 528 SRKHAVACGPGAVKHCEMNVW 590
+ + A C AV HC+ +VW
Sbjct: 538 NMETAARC--NAVDHCKRHVW 556
Score = 31.2 bits (69), Expect = 2.9
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +3
Query: 498 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
C+ G C K AV CG AVKHC+ VW
Sbjct: 25 CSGGSAVLCRDVKTAVDCG--AVKHCQQMVW 53
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 54.7 bits (130), Expect = 2e-07
Identities = 31/117 (26%), Positives = 50/117 (42%), Gaps = 2/117 (1%)
Frame = +3
Query: 246 CRLCCKFYNYLQ-TISSKSSHSNLLFGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKS 422
C +C K YL+ + S+ +L L C+ + + +C + + ++
Sbjct: 439 CEVCKKLVIYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVE 498
Query: 423 KTINEF-CEMTKMCSHQRKNPLGKNPCTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
F C +C K LG C WGPG+WC + + A C AV HC+ +VW
Sbjct: 499 VMDPSFVCSKIGVCPSAYKLLLGTEKCVWGPGYWCQNSETAARC--NAVDHCKRHVW 553
Score = 37.4 bits (85), Expect = 0.041
Identities = 16/43 (37%), Positives = 30/43 (69%), Gaps = 1/43 (2%)
Frame = +3
Query: 72 QQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLCNSTIKQVI 197
Q+QC + +Y L++ I+VE ++PS +CS++G+C S K ++
Sbjct: 478 QKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPSAYKLLL 520
Score = 30.0 bits (66), Expect = 6.5
Identities = 28/150 (18%), Positives = 61/150 (40%), Gaps = 22/150 (14%)
Frame = +3
Query: 81 CTNDINQYYSLVVNIIVEFLNPSLCSELGLCNSTIKQVIMRQ------------PLVQIN 224
C N ++QY + V +++ +C +G C+ +K+V MR P +++
Sbjct: 240 CKNYVDQYSEVAVQMMMHMQPKEICVMVGFCDE-VKRVPMRTLVPATEAIKNILPALELT 298
Query: 225 --------RSKKTSNCRLCCKFYNYL-QTISSKSSHSNLLFGLVSFCNQISAHERLECLK 377
+++ C++C L + I + ++ L+ GL C+ + A +C +
Sbjct: 299 DPYEQDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGLSKACSLLPAPASTKCQE 358
Query: 378 QFRNFN-AVIAAFNKSKTINEFCEMTKMCS 464
F +++ N C + +CS
Sbjct: 359 VLVTFGPSLLDVLMHEVNPNFLCGVISLCS 388
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 37.7 bits (86), Expect = 0.031
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +3
Query: 498 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
C GP FWC S +HAV C A+ HC VW
Sbjct: 31 CAQGPQFWCQSLEHAVQC--RALGHCLQEVW 59
>sp|P17129|PSPB_CANFA Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (Pulmonary surfactant protein 18) (SP 18)
Length = 363
Score = 33.9 bits (76), Expect = 0.45
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +3
Query: 498 CTWGPGFWCVSRKHAVACGPGAVKHCEMNVW 590
C GP FWC S + A+ C A+ HC VW
Sbjct: 25 CARGPAFWCQSLEQALQC--RALGHCLQEVW 53
Score = 30.8 bits (68), Expect = 3.8
Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
Frame = +3
Query: 12 FQKIFENFLINE--ICPHFKTKQQQCTNDINQYYSLVVNIIVEFLNPS-LCSELGLC 173
FQ + FL +E + P K QC + + Y+ +VV+ +NP +C LGLC
Sbjct: 81 FQDMVRKFLEHECDVLP-LKLLTPQCHHMLGTYFPVVVDYFQSQINPKIICKHLGLC 136
>sp|Q9H000|MKRN2_HUMAN Makorin-2 (RING finger protein 62)
Length = 416
Score = 32.7 bits (73), Expect = 1.0
Identities = 25/94 (26%), Positives = 43/94 (45%), Gaps = 3/94 (3%)
Frame = +3
Query: 168 LCNSTIKQVIMRQPLVQINRSKKTSNCRLCCKFYNYLQTISSKSSHSNLLFGLVSFCNQ- 344
+C T + + + Q ++ K S C ++ I K+S S FG++S CN
Sbjct: 215 ICMLTFEHEMEKAFAFQASQDKVCSIC---------MEVILEKASASERRFGILSNCNHT 265
Query: 345 --ISAHERLECLKQFRNFNAVIAAFNKSKTINEF 440
+S + C KQF N +I + + + I+EF
Sbjct: 266 YCLSCIRQWRCAKQFE--NPIIKSCPECRVISEF 297
>sp|Q5DU02|UBP22_MOUSE Ubiquitin carboxyl-terminal hydrolase 22 (Ubiquitin thiolesterase
22) (Ubiquitin-specific processing protease 22)
(Deubiquitinating enzyme 22)
Length = 525
Score = 30.8 bits (68), Expect = 3.8
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = -3
Query: 662 CELKNSSSL---KSSIRIVELYNGHLSPHIHFTMFHCTGT 552
CE+++ SS + S E Y+GH SPHI + + H T
Sbjct: 211 CEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWT 250
>sp|P18745|ZO22_XENLA Oocyte zinc finger protein XLCOF22
Length = 435
Score = 30.8 bits (68), Expect = 3.8
Identities = 29/136 (21%), Positives = 58/136 (42%), Gaps = 2/136 (1%)
Frame = +3
Query: 144 PSLCSELGLCNSTIKQVIMRQPLVQINRSKKTSNCRLCCKFY--NYLQTISSKSSHSNLL 317
P CSE G S + + Q + + +K +C C K + + + + +H+
Sbjct: 293 PFSCSECGKSFSNQSCLRVHQ---RTHTGEKPYSCSECGKSFVTSSQLAVHRRRTHTGEK 349
Query: 318 FGLVSFCNQISAHERLECLKQFRNFNAVIAAFNKSKTINEFCEMTKMCSHQRKNPLGKNP 497
S C + +++ CL+ + + F+ S+ F +K+ SHQR+ G+ P
Sbjct: 350 PFSCSECGKCFSNQ--SCLRVHQRTHTGENLFSCSECGKSFVTSSKLASHQRQTHTGEKP 407
Query: 498 CTWGPGFWCVSRKHAV 545
+ C +RK ++
Sbjct: 408 VSCSECGKCFTRKRSL 423
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,020,113
Number of Sequences: 369166
Number of extensions: 1709214
Number of successful extensions: 4382
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4373
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6462248555
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)