Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_A23
(619 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q01405|SC23A_MOUSE Protein transport protein Sec23A (SEC... 231 1e-60
sp|Q9D662|SC23B_MOUSE Protein transport protein Sec23B (SEC... 230 2e-60
sp|Q15436|SC23A_HUMAN Protein transport protein Sec23A (SEC... 230 2e-60
sp|Q15437|SC23B_HUMAN Protein transport protein Sec23B (SEC... 223 3e-58
sp|P15303|SEC23_YEAST Protein transport protein SEC23 165 8e-41
sp|P38769|YHJ5_YEAST Hypothetical 72.3 kDa protein in SLT2-... 61 3e-09
sp|Q75E74|ATG2_ASHGO Autophagy-related protein 2 32 1.7
sp|O94855|SC24D_HUMAN Protein transport protein Sec24D (SEC... 31 2.9
sp|Q7NBM9|SYN_MYCGA Asparaginyl-tRNA synthetase (Asparagine... 30 5.0
sp|Q9CQX5|CC004_MOUSE Protein C3orf4 homolog 30 6.5
>sp|Q01405|SC23A_MOUSE Protein transport protein Sec23A (SEC23-related protein A)
Length = 765
Score = 231 bits (589), Expect = 1e-60
Identities = 111/182 (60%), Positives = 140/182 (76%), Gaps = 2/182 (1%)
Frame = +1
Query: 79 PSNKFLQPLGECDMVLTDLIEELQPDSWPVGQGKRPLRCVGAALNMAISLLEATYPNTVA 258
PSN+FLQP+ + DM LTDL+ ELQ D WPV QGKRPLR G AL++A+ LLE T+PNT A
Sbjct: 225 PSNRFLQPVQKIDMNLTDLLGELQRDPWPVPQGKRPLRSSGVALSIAVGLLECTFPNTGA 284
Query: 259 RIMTFIGGPCTIGPGLIIDDDLKNIIRSHHDIEKDDCPYMKKAIKHYESLANRAAQNGFV 438
RIM FIGGP T GPG+++ D+LK IRS HDIEKD+ Y+KK KH+E+LANRAA G V
Sbjct: 285 RIMMFIGGPATQGPGMVVGDELKTPIRSWHDIEKDNAKYVKKGTKHFEALANRAATTGHV 344
Query: 439 VDIFSCSLDQTGLHEMRFLPNYTGGHMIMADSFESQLFQQSFKRVFAMD--NTFRMDFGA 612
+DI++C+LDQTGL EM+ PN TGG+M+M DSF + LF+Q+F+RVF D F+M FG
Sbjct: 345 IDIYACALDQTGLLEMKCCPNLTGGYMVMGDSFNTSLFKQTFQRVFTKDIHGQFKMGFGG 404
Query: 613 TI 618
T+
Sbjct: 405 TL 406
>sp|Q9D662|SC23B_MOUSE Protein transport protein Sec23B (SEC23-related protein B)
Length = 767
Score = 230 bits (586), Expect = 2e-60
Identities = 111/181 (61%), Positives = 139/181 (76%), Gaps = 2/181 (1%)
Frame = +1
Query: 82 SNKFLQPLGECDMVLTDLIEELQPDSWPVGQGKRPLRCVGAALNMAISLLEATYPNTVAR 261
S++FLQP+ + DM LTDL+ ELQ D WPV QGKRPLR G AL++A+ LLE T+PNT AR
Sbjct: 228 SSRFLQPIHKIDMNLTDLLGELQRDPWPVTQGKRPLRSTGVALSIAVGLLEGTFPNTGAR 287
Query: 262 IMTFIGGPCTIGPGLIIDDDLKNIIRSHHDIEKDDCPYMKKAIKHYESLANRAAQNGFVV 441
IM F GGP T GPG+++ D+LK IRS HDIEKD+ +MKKA KHYE LANR A NG +
Sbjct: 288 IMLFTGGPPTQGPGMVVGDELKTPIRSWHDIEKDNARFMKKATKHYEMLANRTATNGHCI 347
Query: 442 DIFSCSLDQTGLHEMRFLPNYTGGHMIMADSFESQLFQQSFKRVFAMD--NTFRMDFGAT 615
DI++C+LDQTGL EM+ PN TGGHM+M DSF + LF+Q+F+R+F+ D FRM FGAT
Sbjct: 348 DIYACALDQTGLLEMKCCPNLTGGHMVMGDSFNTSLFKQTFQRIFSKDFNGDFRMAFGAT 407
Query: 616 I 618
+
Sbjct: 408 L 408
>sp|Q15436|SC23A_HUMAN Protein transport protein Sec23A (SEC23-related protein A)
Length = 765
Score = 230 bits (586), Expect = 2e-60
Identities = 110/182 (60%), Positives = 140/182 (76%), Gaps = 2/182 (1%)
Frame = +1
Query: 79 PSNKFLQPLGECDMVLTDLIEELQPDSWPVGQGKRPLRCVGAALNMAISLLEATYPNTVA 258
PSN+FLQP+ + DM LTDL+ ELQ D WPV QGKRPLR G AL++A+ LLE T+PNT A
Sbjct: 225 PSNRFLQPVQKIDMNLTDLLGELQRDPWPVPQGKRPLRSSGVALSIAVGLLECTFPNTGA 284
Query: 259 RIMTFIGGPCTIGPGLIIDDDLKNIIRSHHDIEKDDCPYMKKAIKHYESLANRAAQNGFV 438
RIM FIGGP T GPG+++ D+LK IRS HDI+KD+ Y+KK KH+E+LANRAA G V
Sbjct: 285 RIMMFIGGPATQGPGMVVGDELKTPIRSWHDIDKDNAKYVKKGTKHFEALANRAATTGHV 344
Query: 439 VDIFSCSLDQTGLHEMRFLPNYTGGHMIMADSFESQLFQQSFKRVFAMD--NTFRMDFGA 612
+DI++C+LDQTGL EM+ PN TGG+M+M DSF + LF+Q+F+RVF D F+M FG
Sbjct: 345 IDIYACALDQTGLLEMKCCPNLTGGYMVMGDSFNTSLFKQTFQRVFTKDMHGQFKMGFGG 404
Query: 613 TI 618
T+
Sbjct: 405 TL 406
>sp|Q15437|SC23B_HUMAN Protein transport protein Sec23B (SEC23-related protein B)
Length = 767
Score = 223 bits (568), Expect = 3e-58
Identities = 111/188 (59%), Positives = 139/188 (73%), Gaps = 3/188 (1%)
Frame = +1
Query: 64 PASH-LPSNKFLQPLGECDMVLTDLIEELQPDSWPVGQGKRPLRCVGAALNMAISLLEAT 240
P H S++FLQP+ + DM LTDL+ ELQ D WPV QGKRPLR G AL++A+ LLE T
Sbjct: 221 PQEHPFASSRFLQPVHKIDMNLTDLLGELQRDPWPVTQGKRPLRSTGVALSIAVGLLEGT 280
Query: 241 YPNTVARIMTFIGGPCTIGPGLIIDDDLKNIIRSHHDIEKDDCPYMKKAIKHYESLANRA 420
+PNT ARIM F GGP T GPG+++ D+LK IRS HDIEKD+ +MKKA KHYE LANR
Sbjct: 281 FPNTGARIMLFTGGPPTQGPGMVVGDELKIPIRSWHDIEKDNARFMKKATKHYEMLANRT 340
Query: 421 AQNGFVVDIFSCSLDQTGLHEMRFLPNYTGGHMIMADSFESQLFQQSFKRVFAMD--NTF 594
A NG +DI++C+LDQTGL EM+ N TGG+M+M DSF + LF+Q+F+R+F D F
Sbjct: 341 AANGHCIDIYACALDQTGLLEMKCCANLTGGYMVMGDSFNTSLFKQTFQRIFTKDFNGDF 400
Query: 595 RMDFGATI 618
RM FGAT+
Sbjct: 401 RMAFGATL 408
>sp|P15303|SEC23_YEAST Protein transport protein SEC23
Length = 768
Score = 165 bits (418), Expect = 8e-41
Identities = 83/183 (45%), Positives = 114/183 (62%), Gaps = 10/183 (5%)
Frame = +1
Query: 67 ASHLPS----------NKFLQPLGECDMVLTDLIEELQPDSWPVGQGKRPLRCVGAALNM 216
ASHLP+ N+F PL + + L L+E L PD W V G RPLR G+ALN+
Sbjct: 209 ASHLPNAMNKVTPFSLNRFFLPLEQVEFKLNQLLENLSPDQWSVPAGHRPLRATGSALNI 268
Query: 217 AISLLEATYPNTVARIMTFIGGPCTIGPGLIIDDDLKNIIRSHHDIEKDDCPYMKKAIKH 396
A LL+ Y N ARI+ F GP T+ PGLI++ +LK+ +RSHHDI+ D + KKA K
Sbjct: 269 ASLLLQGCYKNIPARIILFASGPGTVAPGLIVNSELKDPLRSHHDIDSDHAQHYKKACKF 328
Query: 397 YESLANRAAQNGFVVDIFSCSLDQTGLHEMRFLPNYTGGHMIMADSFESQLFQQSFKRVF 576
Y +A R A NG VDIF+ DQ G+ EM+ L + TGG +++ D+F + +F+QS+ R+F
Sbjct: 329 YNQIAQRVAANGHTVDIFAGCYDQIGMSEMKQLTDSTGGVLLLTDAFSTAIFKQSYLRLF 388
Query: 577 AMD 585
A D
Sbjct: 389 AKD 391
>sp|P38769|YHJ5_YEAST Hypothetical 72.3 kDa protein in SLT2-PUT2 intergenic region
Length = 630
Score = 60.8 bits (146), Expect = 3e-09
Identities = 39/134 (29%), Positives = 60/134 (44%), Gaps = 3/134 (2%)
Frame = +1
Query: 178 KRPLRCVGAALNMAISLLEATYPNTVARIMTFIGGPCTIGPGLIIDDDLKNIIRSHHDIE 357
KR RC G AL ++ L + I++F+ GPCT G G ++ + +R +H E
Sbjct: 199 KRDKRCTGLALFVSSVLASQCSLSAYCHIVSFLNGPCTKGGGKVMSRERGESMRQNHHFE 258
Query: 358 --KDDCPYMKKAIKHYESLANRAAQNGFVVDIFSCSLDQTGLHEMRFLPNYTGGHMI-MA 528
K K Y+ + + A + + F SLDQ G+ EM P T +
Sbjct: 259 SKSSQLQLSKSPTKFYKKMLEKFANQSLIYEFFIASLDQIGILEMS--PLITSSMAVSQF 316
Query: 529 DSFESQLFQQSFKR 570
DSF + F SF++
Sbjct: 317 DSFNDERFAMSFQK 330
>sp|Q75E74|ATG2_ASHGO Autophagy-related protein 2
Length = 1525
Score = 31.6 bits (70), Expect = 1.7
Identities = 21/57 (36%), Positives = 32/57 (56%), Gaps = 5/57 (8%)
Frame = +1
Query: 319 DLKNIIRSHHDIEKDDCPYMKKAI-KHYESLANRAAQNGFVV----DIFSCSLDQTG 474
+ K IIRS + DDC MK +I + +ESL ++ GF V + SC++ Q+G
Sbjct: 783 NFKCIIRSPTFMLADDCSNMKTSITESWESLVRYYSKAGFAVIGKSGLLSCTIKQSG 839
>sp|O94855|SC24D_HUMAN Protein transport protein Sec24D (SEC24-related protein D)
Length = 1032
Score = 30.8 bits (68), Expect = 2.9
Identities = 24/110 (21%), Positives = 46/110 (41%)
Frame = +1
Query: 283 PCTIGPGLIIDDDLKNIIRSHHDIEKDDCPYMKKAIKHYESLANRAAQNGFVVDIFSCSL 462
P PG + + D K ++ + +K+ + + Y+SLA +G V +F
Sbjct: 585 PTAEAPGKLKNRDDKKLVNT----DKEKILFQPQT-NVYDSLAKDCVAHGCSVTLFLFPS 639
Query: 463 DQTGLHEMRFLPNYTGGHMIMADSFESQLFQQSFKRVFAMDNTFRMDFGA 612
+ + +P TGG + ++F+ L +Q F D ++ F A
Sbjct: 640 QYVDVASLGLVPQLTGGTLYKYNNFQMHLDRQQFLNDLRNDIEKKIGFDA 689
>sp|Q7NBM9|SYN_MYCGA Asparaginyl-tRNA synthetase (Asparagine--tRNA ligase) (AsnRS)
Length = 457
Score = 30.0 bits (66), Expect = 5.0
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Frame = +1
Query: 496 PNYTG--GHMIMADSFESQLFQQSFKRVFAMDNTFRMD 603
PNY G + ++ F ++ + Q FKRVF TFR +
Sbjct: 184 PNYFGKKASLTVSGQFAAEAYAQGFKRVFTFGPTFRAE 221
>sp|Q9CQX5|CC004_MOUSE Protein C3orf4 homolog
Length = 253
Score = 29.6 bits (65), Expect = 6.5
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +1
Query: 187 LRCVGAALNMAISLLEATYPNTVARIMTFIGGPCTIG 297
L C GA + + + + YP I+ + G CT+G
Sbjct: 150 LMCFGALIGLCACICRSLYPTLATGILHLLAGLCTLG 186
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,641,121
Number of Sequences: 369166
Number of extensions: 1521116
Number of successful extensions: 3510
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3426
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3510
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4895780500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)