Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_A20
(386 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P31226|SAX_RANCA Saxiphilin precursor (SAX) 44 9e-05
sp|Q9BQ16|TICN3_HUMAN Testican-3 precursor (SPARC/osteonect... 43 2e-04
sp|Q8BKV0|TICN3_MOUSE Testican-3 precursor (SPARC/osteonect... 43 2e-04
sp|Q5RD69|TICN3_PONPY Testican-3 precursor (SPARC/osteonect... 43 2e-04
sp|P01266|THYG_HUMAN Thyroglobulin precursor 42 3e-04
sp|P81439|EQST_ACTEQ Equistatin 42 3e-04
sp|Q8CD91|SMOC2_MOUSE SPARC related modular calcium-binding... 41 7e-04
sp|P06882|THYG_RAT Thyroglobulin precursor 41 0.001
sp|O88322|NID2_MOUSE Nidogen-2 precursor (NID-2) (Entactin-2) 40 0.001
sp|Q14112|NID2_HUMAN Nidogen-2 precursor (NID-2) (Osteonido... 40 0.001
>sp|P31226|SAX_RANCA Saxiphilin precursor (SAX)
Length = 844
Score = 44.3 bits (103), Expect = 9e-05
Identities = 34/126 (26%), Positives = 54/126 (42%), Gaps = 11/126 (8%)
Frame = +3
Query: 42 NNDQCNVGLICDEVCKEDNNAPNCFKRHLDARRLIKSGAVDVTFPFCEDTGMYKPVQCVG 221
+ D N+ I E + + C K A + + P C++ G Y+P QC G
Sbjct: 87 SKDPYNLKPIIAEPYSSNRDLQKCLKERQQA---LAKKMIGHYIPQCDEKGNYQPQQCHG 143
Query: 222 AA--CWCVEESGDEIEGYITGIEESYGKTC------NCAKDKLEY--SQRKIVGRTF-RC 368
+ CWCV G++I G T ++ TC C K++ K++GR +C
Sbjct: 144 STGHCWCVNAMGEKISGTNTPPGQTRA-TCERHELPKCLKERQVALGGDEKVLGRFVPQC 202
Query: 369 DHLGDY 386
D G+Y
Sbjct: 203 DEKGNY 208
Score = 32.0 bits (71), Expect = 0.45
Identities = 21/64 (32%), Positives = 29/64 (45%), Gaps = 5/64 (7%)
Frame = +3
Query: 90 EDNNAPNCFKRHLDARRLIKSGAVDVT---FPFCEDTGMYKPVQCVGAA--CWCVEESGD 254
E + P C K R++ G V P C++ G Y+P Q G+ WCV G+
Sbjct: 173 ERHELPKCLKE----RQVALGGDEKVLGRFVPQCDEKGNYEPQQFHGSTGYSWCVNAIGE 228
Query: 255 EIEG 266
EI G
Sbjct: 229 EIAG 232
>sp|Q9BQ16|TICN3_HUMAN Testican-3 precursor (SPARC/osteonectin, CWCV, and Kazal-like
domains proteoglycan 3)
Length = 436
Score = 43.1 bits (100), Expect = 2e-04
Identities = 16/33 (48%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = +3
Query: 174 PFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
P C++ G YKP QC G+ CWCV+ G+E+ G
Sbjct: 339 PLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVMG 371
>sp|Q8BKV0|TICN3_MOUSE Testican-3 precursor (SPARC/osteonectin, CWCV, and Kazal-like
domains proteoglycan 3)
Length = 436
Score = 43.1 bits (100), Expect = 2e-04
Identities = 16/33 (48%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = +3
Query: 174 PFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
P C++ G YKP QC G+ CWCV+ G+E+ G
Sbjct: 339 PLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVVG 371
>sp|Q5RD69|TICN3_PONPY Testican-3 precursor (SPARC/osteonectin, CWCV, and Kazal-like
domains proteoglycan 3)
Length = 436
Score = 43.1 bits (100), Expect = 2e-04
Identities = 16/33 (48%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = +3
Query: 174 PFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
P C++ G YKP QC G+ CWCV+ G+E+ G
Sbjct: 339 PLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVMG 371
>sp|P01266|THYG_HUMAN Thyroglobulin precursor
Length = 2768
Score = 42.4 bits (98), Expect = 3e-04
Identities = 24/70 (34%), Positives = 39/70 (55%), Gaps = 4/70 (5%)
Frame = +3
Query: 174 PFCE-DTGMYKPVQCVGA--ACWCVEESGDEIEG-YITGIEESYGKTCNCAKDKLEYSQR 341
P C + G + PVQC A +CWCV +SG+E+ G +TG + + C + L ++
Sbjct: 1167 PACRAEDGGFSPVQCDQAQGSCWCVMDSGEEVPGTRVTGGQPA----CESPRCPLPFNAS 1222
Query: 342 KIVGRTFRCD 371
++VG T C+
Sbjct: 1223 EVVGGTILCE 1232
Score = 40.8 bits (94), Expect = 0.001
Identities = 20/53 (37%), Positives = 30/53 (56%), Gaps = 6/53 (11%)
Frame = +3
Query: 126 LDARRLIKSGAVDVT----FPFCEDTGMYKPVQC--VGAACWCVEESGDEIEG 266
L ++++ SG ++ T P C+D+G Y PVQC CWCV+ G E+ G
Sbjct: 98 LQKQQILLSGYINSTDTSYLPQCQDSGDYAPVQCDVQQVQCWCVDAEGMEVYG 150
Score = 38.5 bits (88), Expect = 0.005
Identities = 20/50 (40%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +3
Query: 132 ARRLIKSGAVDVTFPFCEDTGMYKPVQCVGAA--CWCVEESGDEIEGYIT 275
A L++SG P C+ G ++PVQC CWCV+E G I G +T
Sbjct: 1018 ASALLRSGPY---MPQCDAFGSWEPVQCHAGTGHCWCVDEKGGFIPGSLT 1064
Score = 37.7 bits (86), Expect = 0.008
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = +3
Query: 174 PFCEDTGMYKPVQCVGAACWCVEESGDEIEG 266
P C G Y+ VQC CWCV G E+ G
Sbjct: 618 PSCTTEGSYEDVQCFSGECWCVNSWGKELPG 648
Score = 36.6 bits (83), Expect = 0.018
Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 2/70 (2%)
Frame = +3
Query: 108 NCFKRHLDARRLIKSGAVDVTF--PFCEDTGMYKPVQCVGAACWCVEESGDEIEGYITGI 281
+C K+ + L+ S T P C G + PVQC + C+CV+ G I G + I
Sbjct: 661 DCEKQRARMQSLMGSQPAGSTLFVPACTSEGHFLPVQCFNSECYCVDAEGQAIPGTRSAI 720
Query: 282 EESYGKTCNC 311
GK C
Sbjct: 721 ----GKPKKC 726
Score = 35.4 bits (80), Expect = 0.040
Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = +3
Query: 174 PFCEDTGMYKPVQC-VGAACWCVEESGDEIEGYITGIEESYGKTCNCAKDKLEYSQRK 344
P C G Y+ VQC CWCV+ G E+ G G+ +CA+ + S+R+
Sbjct: 317 PSCRRNGDYQAVQCQTEGPCWCVDAQGKEMHG-----TRQQGEPPSCAEGQSCASERQ 369
Score = 33.9 bits (76), Expect = 0.12
Identities = 20/64 (31%), Positives = 27/64 (42%), Gaps = 1/64 (1%)
Frame = +3
Query: 72 CDEVCKEDNNAPNCFKRHLDARRLIKSGAVDVTFPFCEDTGMYKPVQCVGAACWCVE-ES 248
C C E + AR D+ P C +TG Y +Q GA WCV+ S
Sbjct: 1073 CPTTC-EKSRTSGLLSSWKQARSQENPSPKDLFVPACLETGEYARLQASGAGTWCVDPAS 1131
Query: 249 GDEI 260
G+E+
Sbjct: 1132 GEEL 1135
Score = 31.6 bits (70), Expect = 0.58
Identities = 17/39 (43%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = +3
Query: 228 CWCVEESGDEIEGYITGIEESYGKTC--NCAKDKLEYSQ 338
CWCV+E+G E+EG + E S TC +C + KL Q
Sbjct: 898 CWCVDEAGQELEGMRS--EPSKLPTCPGSCEEAKLRVLQ 934
>sp|P81439|EQST_ACTEQ Equistatin
Length = 199
Score = 42.4 bits (98), Expect = 3e-04
Identities = 31/95 (32%), Positives = 45/95 (47%), Gaps = 13/95 (13%)
Frame = +3
Query: 21 EEFDVCGNNDQCNVGLICDEVCKE-----DNNAPNCFKRHL----DARRLIKSGAVDVTF 173
+E C +N +C D+ KE P+C +RHL +AR S ++ V
Sbjct: 99 DEVQCCASNGEC---YCVDKKGKELEGTRQKGRPSC-ERHLSPCEEARLQAHSNSLRVGM 154
Query: 174 --PFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
P C + G Y PVQC + CWCV+E G ++ G
Sbjct: 155 FVPQCLEDGSYNPVQCWPSTGYCWCVDEGGVKVPG 189
Score = 41.6 bits (96), Expect = 6e-04
Identities = 18/41 (43%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Frame = +3
Query: 150 SGAVDVTFPFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
SG + P C++TG ++ QC G+ CWCV+E G EI G
Sbjct: 14 SGLIGAYVPQCKETGEFEEKQCWGSTGYCWCVDEDGKEILG 54
>sp|Q8CD91|SMOC2_MOUSE SPARC related modular calcium-binding protein 2 precursor (Secreted
modular calcium-binding protein 2) (SMOC-2)
Length = 447
Score = 41.2 bits (95), Expect = 7e-04
Identities = 26/68 (38%), Positives = 34/68 (50%), Gaps = 4/68 (5%)
Frame = +3
Query: 96 NNAPNCFKRHLDARRLIKSGAVD-VTFPFCEDTGMYKPVQCVGAA--CWCV-EESGDEIE 263
N+A +C + H A K D V P C G+YKPVQC + CWCV ++G I
Sbjct: 211 NSASSCDQEHQSALEEAKQPKNDNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIP 270
Query: 264 GYITGIEE 287
G T E+
Sbjct: 271 GTSTRYEQ 278
Score = 33.9 bits (76), Expect = 0.12
Identities = 16/36 (44%), Positives = 17/36 (47%), Gaps = 2/36 (5%)
Frame = +3
Query: 165 VTFPFCEDTGMYKPVQC--VGAACWCVEESGDEIEG 266
V P C D G Y VQC CWCV +G I G
Sbjct: 108 VFIPECNDDGTYSQVQCHSYTGYCWCVTPNGRPISG 143
>sp|P06882|THYG_RAT Thyroglobulin precursor
Length = 2768
Score = 40.8 bits (94), Expect = 0.001
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +3
Query: 174 PFCEDTGMYKPVQCVGAACWCVEESGDEIEG 266
P C G Y+ +QC CWCV G E+EG
Sbjct: 618 PSCTAEGSYEDIQCYAGECWCVNSQGKEVEG 648
Score = 40.0 bits (92), Expect = 0.002
Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 6/53 (11%)
Frame = +3
Query: 126 LDARRLIKSGAVDVT----FPFCEDTGMYKPVQC--VGAACWCVEESGDEIEG 266
L +R++ S ++ T P C+D+G Y PVQC CWCV+ G E+ G
Sbjct: 99 LHKQRILLSSYINSTDALYLPQCQDSGNYAPVQCDLQQVQCWCVDTEGMEVYG 151
Score = 38.9 bits (89), Expect = 0.004
Identities = 22/58 (37%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +3
Query: 174 PFCEDTGMYKPVQC-VGAACWCVEESGDEIEGYITGIEESYGKTCNCAKDKLEYSQRK 344
P C G Y+ VQC + CWCV+ G EI G G+ CAKD+ S+R+
Sbjct: 318 PSCHRDGHYQTVQCQMERMCWCVDAQGIEIPG-----TRQQGQPLFCAKDQSCASERQ 370
Score = 37.4 bits (85), Expect = 0.011
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +3
Query: 162 DVTFPFCEDTGMYKPVQCV--GAACWCVEESGDEIEG 266
D P C + G ++ VQC G +CWCV+ G E+ G
Sbjct: 47 DEYVPQCSEDGSFQTVQCQNDGQSCWCVDSDGTEVPG 83
Score = 36.2 bits (82), Expect = 0.024
Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = +3
Query: 111 CFKRHLDARRLIKSGAVDVTF--PFCEDTGMYKPVQCVGAACWCVEESGDEIEGYITGIE 284
C K+ + L + +F P C G + PVQC + C+CV+ G I G + I
Sbjct: 662 CEKQRAQMQNLAGAQPAGSSFFVPTCTSEGYFLPVQCFNSECYCVDAEGQVIPGTQSTIG 721
Query: 285 E 287
E
Sbjct: 722 E 722
Score = 33.9 bits (76), Expect = 0.12
Identities = 16/34 (47%), Positives = 20/34 (58%), Gaps = 3/34 (8%)
Frame = +3
Query: 174 PFCEDT-GMYKPVQC--VGAACWCVEESGDEIEG 266
P CE G + PVQC +CWCV SG+E+ G
Sbjct: 1168 PVCEALDGGFSPVQCDLAQGSCWCVLASGEEVPG 1201
Score = 32.0 bits (71), Expect = 0.45
Identities = 22/75 (29%), Positives = 31/75 (41%)
Frame = +3
Query: 153 GAVDVTFPFCEDTGMYKPVQCVGAACWCVEESGDEIEGYITGIEESYGKTCNCAKDKLEY 332
G D+ P C TG Y Q G WCV+ S E T C+ K ++
Sbjct: 1100 GPGDLFTPVCLQTGEYVRQQTSGTGAWCVDPSSGEGVPTNTNSSAQCPGLCDALKSRV-L 1158
Query: 333 SQRKIVGRTFRCDHL 377
S++ +G T C+ L
Sbjct: 1159 SRKVGLGYTPVCEAL 1173
Score = 30.8 bits (68), Expect = 1.00
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +3
Query: 174 PFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
P C G ++PVQC CWCV+ G+ I G
Sbjct: 1030 PQCNTIGGWEPVQCHPGTGQCWCVDGWGELIPG 1062
Score = 28.9 bits (63), Expect = 3.8
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +3
Query: 225 ACWCVEESGDEIEG 266
+CWCV+E+G E++G
Sbjct: 898 SCWCVDEAGQELDG 911
>sp|O88322|NID2_MOUSE Nidogen-2 precursor (NID-2) (Entactin-2)
Length = 1403
Score = 40.4 bits (93), Expect = 0.001
Identities = 15/36 (41%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
Frame = +3
Query: 165 VTFPFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
+ P C+D G + P+QC G+ CWCV+ +G E+ G
Sbjct: 986 IHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPG 1021
Score = 40.0 bits (92), Expect = 0.002
Identities = 16/37 (43%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Frame = +3
Query: 162 DVTFPFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
D P C+D G + P+QC G + CWCV++ G E++G
Sbjct: 1065 DQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELQG 1101
>sp|Q14112|NID2_HUMAN Nidogen-2 precursor (NID-2) (Osteonidogen)
Length = 1375
Score = 40.4 bits (93), Expect = 0.001
Identities = 16/37 (43%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Frame = +3
Query: 162 DVTFPFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
D P C+D G + P+QC G + CWCV++ G E++G
Sbjct: 1037 DQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQG 1073
Score = 37.7 bits (86), Expect = 0.008
Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +3
Query: 174 PFCEDTGMYKPVQCVGAA--CWCVEESGDEIEG 266
P C++ G + P+QC G+ CWCV+ G E+ G
Sbjct: 961 PQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPG 993
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,456,665
Number of Sequences: 369166
Number of extensions: 877004
Number of successful extensions: 2630
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2441
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2583
length of database: 68,354,980
effective HSP length: 95
effective length of database: 50,805,155
effective search space used: 1676570115
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)