Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_P12
(886 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-r... 44 8e-04
sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding... 35 0.21
sp|Q99541|ADFP_HUMAN Adipophilin (Adipose differentiation-r... 34 0.47
sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2) 32 3.1
sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HS... 32 3.1
sp|P57498|CYSN_BUCAI Sulfate adenylyltransferase subunit 1 ... 32 3.1
sp|P03589|V125_AMVLE 125 kDa protein 31 4.0
sp|Q06412|TUS1_YEAST Rho1 guanine nucleotide exchange facto... 31 4.0
sp|P80872|G16O_BACSU General stress protein 16O (GSP16O) 31 4.0
sp|P33126|HSP82_ORYSA Heat shock protein 82 31 5.2
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 425
Score = 43.5 bits (101), Expect = 8e-04
Identities = 56/290 (19%), Positives = 112/290 (38%), Gaps = 13/290 (4%)
Frame = +3
Query: 27 RIREYPVIKDTFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS------- 185
R+ P++ T+ Y K++ L S E +K V A
Sbjct: 15 RVANLPLVSSTYDLVSSAYVSTKDQYPYLRSVCEMAEKGVKTVTSAAMTSALPIIQKLEP 74
Query: 186 KLGSVDNFACNQLDKLETKYPXXXXXXXXXXXXXGEYYDSSIKPNVDKMIAAKDSAVYIA 365
++ + +AC LD++E + P + +++A+ AV A
Sbjct: 75 QIAVANTYACKGLDRMEERLPI-------------------LNQPTSEIVASARGAVTGA 115
Query: 366 KTPFRITGQTIEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFT 545
K + T+ ++ S V + K K A G+VE+ + +++ +
Sbjct: 116 KD---VVTTTMAGAKDSVASTVSGVVDKTKGAVTGSVERTK-------------SVVNGS 159
Query: 546 LKNIEGLSNVLNSIYDQSVTKNKEWLHGYY----EEFTDEMKKNSGDESKTESTVMQLAQ 713
+ + G+ +NS D ++TK++ + Y+ EE E KK G + + + + +
Sbjct: 160 INTVLGMVQFMNSGVDNAITKSEMLVDQYFPLTQEELEMEAKKVEGFDMVQKPSNYERLE 219
Query: 714 NFSNKL--RVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTY 857
+ S KL R +A+S V ++ET+ + T H+ K+ +
Sbjct: 220 SLSTKLCSRAYHQALSRV--KEAKQKSQETIS-QLHSTVHLIEFARKNMH 266
>sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding protein 2 (Lsd2)
Length = 352
Score = 35.4 bits (80), Expect = 0.21
Identities = 24/82 (29%), Positives = 35/82 (42%), Gaps = 3/82 (3%)
Frame = +3
Query: 12 IKSLERIREYPVIKDTFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS-- 185
++SLERI + PV+ + K+ + Y +K KN LT E + P
Sbjct: 37 LESLERIIKLPVVNAAWDKSQDVYGKVKGKNRVFEWALTAAEDCVTRAVTTAAPFVTKLD 96
Query: 186 -KLGSVDNFACNQLDKLETKYP 248
+ VD +DKLE K P
Sbjct: 97 RPIAYVDQTLVKGIDKLEVKAP 118
>sp|Q99541|ADFP_HUMAN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 437
Score = 34.3 bits (77), Expect = 0.47
Identities = 58/294 (19%), Positives = 113/294 (38%), Gaps = 12/294 (4%)
Frame = +3
Query: 27 RIREYPVIKDTFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS------- 185
R+ P++ T+ Y K++ L S E+ +K + + A
Sbjct: 15 RVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEP 74
Query: 186 KLGSVDNFACNQLDKLETKYPXXXXXXXXXXXXXGEYYDSSIKPNVDKMIAAKDSAVYIA 365
++ + +AC LD++E + P + +++A AV A
Sbjct: 75 QIAVANTYACKGLDRIEERLPI-------------------LNQPSTQIVANAKGAVTGA 115
Query: 366 KTPFRITGQTIEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFT 545
K T+ ++ S + M K K A G+VEK + + +NT+L
Sbjct: 116 KD---AVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVV-----SGSINTVLGSR 167
Query: 546 LKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTESTVMQLAQNFSN 725
+ + +S+ + + +S +++L EE E KK G + + + + S
Sbjct: 168 MMQL--VSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLST 225
Query: 726 KL--RVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTY---QEIRD 872
KL R +A+S V +++T+ + T H+ K+ Y Q+I+D
Sbjct: 226 KLHSRAYQQALSRV--KEAKQKSQQTIS-QLHSTVHLIEFARKNVYSANQKIQD 276
>sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2)
Length = 699
Score = 31.6 bits (70), Expect = 3.1
Identities = 20/87 (22%), Positives = 42/87 (48%)
Frame = +3
Query: 468 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 647
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 648 DEMKKNSGDESKTESTVMQLAQNFSNK 728
+K ++S+ + + +L + S K
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTK 440
>sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HSP81.2)
Length = 699
Score = 31.6 bits (70), Expect = 3.1
Identities = 20/87 (22%), Positives = 42/87 (48%)
Frame = +3
Query: 468 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 647
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 648 DEMKKNSGDESKTESTVMQLAQNFSNK 728
+K ++S+ + + +L + S K
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTK 440
>sp|P57498|CYSN_BUCAI Sulfate adenylyltransferase subunit 1 (Sulfate adenylate
transferase) (SAT) (ATP-sulfurylase large subunit)
Length = 473
Score = 31.6 bits (70), Expect = 3.1
Identities = 42/160 (26%), Positives = 73/160 (45%), Gaps = 15/160 (9%)
Frame = +3
Query: 387 GQTIEFLSNTTNSFVHRFMSKD---KKAEYG----AVEKKEYDILYRRALKEVNTILTFT 545
GQTI+ L NS V R ++ D KKAE G V K E DI +++IL +
Sbjct: 269 GQTIKILPENINSRVSRIVTFDKELKKAEIGESITVVLKDEIDINRGDFFVNIDSILQPS 328
Query: 546 LKNIEGL----SNVLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTESTVMQLAQ 713
+ I + N+L + +V + + + Y +E ++ N+ + K+ S V+
Sbjct: 329 QEAIIDIVWMTDNILLAGESYNVKLSGKKIRVYIKEILFKLDVNTLKKVKSHSLVL---- 384
Query: 714 NFSNKLRVSVRAMSTVCYNHMS----SGNKETLDLMFNKT 821
++ RV + V +++ S +GN +DL+ N T
Sbjct: 385 --NSIGRVKIYFSKPVIFDNYSENRMTGNMIFIDLLTNIT 422
>sp|P03589|V125_AMVLE 125 kDa protein
Length = 1126
Score = 31.2 bits (69), Expect = 4.0
Identities = 28/113 (24%), Positives = 50/113 (44%), Gaps = 10/113 (8%)
Frame = +3
Query: 498 LYRRALKEVNTILTFTLKNIEGLSNVLNS-IYDQSV--TKNKEWLHG-------YYEEFT 647
LY+ K++ T+ +F L I+G S +++ +Y QS+ EW G Y
Sbjct: 556 LYQSFQKKIETVFSFILGKIDGPSPLISDPVYFQSLEDVYYAEWHQGNAIDASNYARTLL 615
Query: 648 DEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDL 806
D+++K + K ++ ++ AQ KL ++ + H G E L L
Sbjct: 616 DDIRKQKEESLKAKAKEVEDAQ----KLNRAILQVHAYLEAHPDGGKIEGLGL 664
>sp|Q06412|TUS1_YEAST Rho1 guanine nucleotide exchange factor TUS1 (TOR unique function
suppressor protein 1)
Length = 1307
Score = 31.2 bits (69), Expect = 4.0
Identities = 30/118 (25%), Positives = 53/118 (44%), Gaps = 4/118 (3%)
Frame = +3
Query: 471 AVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNV----LNSIYDQSVTKNKEWLHGYYE 638
AV K+ ILY+ + ++ +L L + + ++++ LNS+ +S T +K+ E
Sbjct: 1108 AVCTKKTVILYQDSFEDNGIVLPSFLNDKDMMAHLRHPHLNSLPFKSATDSKK--RPSIE 1165
Query: 639 EFTDEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMF 812
T+E KK+ Q++Q+ L + CY MS K+ L L F
Sbjct: 1166 SLTEEAKKDIATCKAIPVNFFQISQSSFFALVYDEAVVKINCYGEMSDWRKDILLLDF 1223
>sp|P80872|G16O_BACSU General stress protein 16O (GSP16O)
Length = 163
Score = 31.2 bits (69), Expect = 4.0
Identities = 22/105 (20%), Positives = 47/105 (44%)
Frame = +3
Query: 378 RITGQTIEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFTLKNI 557
R+T QT++ + V+R + K K YG EK +I Y R + + +
Sbjct: 57 RMTDQTVKEIDRELLEEVNRALQKMKDGTYGVCEKTGQEIPYERL-----EAVPYARMTV 111
Query: 558 EGLSNVLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTES 692
E ++V + + + + +E ++E+ D K + D+ +++
Sbjct: 112 EAQADVEDDLETDAPSYERE----FHEQVKDLSNKETIDQKSSQT 152
>sp|P33126|HSP82_ORYSA Heat shock protein 82
Length = 699
Score = 30.8 bits (68), Expect = 5.2
Identities = 19/87 (21%), Positives = 42/87 (48%)
Frame = +3
Query: 468 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 647
G V+ ++ + R + + N IL KN+ + + ++ + +NKE + +YE F+
Sbjct: 360 GIVDSEDLPLNISREMLQQNKILKVIRKNL--VKKCVELFFE--IAENKEDYNKFYEAFS 415
Query: 648 DEMKKNSGDESKTESTVMQLAQNFSNK 728
+K ++S + + +L + S K
Sbjct: 416 KNLKLGIHEDSTNRNKIAELLRYHSTK 442
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,396,425
Number of Sequences: 369166
Number of extensions: 1928965
Number of successful extensions: 5597
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5300
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5594
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8838279920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)