Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_N20
(807 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P93214|14339_LYCES 14-3-3 protein 9 291 1e-78
sp|Q9C5W6|143C_ARATH 14-3-3-like protein GF14 iota (General... 290 2e-78
sp|Q96453|1433D_SOYBN 14-3-3-like protein D (SGF14D) 289 5e-78
sp|P48347|14310_ARATH 14-3-3-like protein GF14 epsilon (Gen... 289 7e-78
sp|P93212|1437_LYCES 14-3-3 protein 7 288 9e-78
sp|Q99002|1433_TRIHA 14-3-3 protein homolog (Th1433) 288 2e-77
sp|P34730|BMH2_YEAST Protein BMH2 287 2e-77
sp|P42650|14333_ENTHI 14-3-3 protein 3 (14-3-3-3) 287 2e-77
sp|P42654|1433B_VICFA 14-3-3-like protein B (VFA-1433B) 287 3e-77
sp|P92177|1433E_DROME 14-3-3 protein epsilon (Suppressor of... 287 3e-77
>sp|P93214|14339_LYCES 14-3-3 protein 9
Length = 261
Score = 291 bits (745), Expect = 1e-78
Identities = 154/247 (62%), Positives = 192/247 (77%), Gaps = 5/247 (2%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKLAEQAERYDEMV++MK VA D EL+VEERNLLSV +KNV+GSRRASWRILS+IEQ+E
Sbjct: 14 AKLAEQAERYDEMVEAMKNVANMDVELTVEERNLLSVGYKNVVGSRRASWRILSSIEQKE 73
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E++G+ Q ++ K Y ++VE EL +C++++ V+D L+P S ES VFY KMKGDYY
Sbjct: 74 ESRGNEQ-NVKRIKEYLQKVESELTNICNDIMVVIDQHLIPSCSAGESTVFYHKMKGDYY 132
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF +++EVA+ SL AY++A T AE+ L+ THPIRLGLALNFSVFY+EIM +PE
Sbjct: 133 RYLAEFKAGNDKKEVAELSLKAYQAATTAAEAELAPTHPIRLGLALNFSVFYYEIMNSPE 192
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNS----GEEDDNKQNET 707
+AC LAK AFD+AI ELD+L E++YKDSTLIMQLLRDNLTLW S ED K + T
Sbjct: 193 RACHLAKQAFDEAISELDSL-NEDSYKDSTLIMQLLRDNLTLWTSDLPEDAEDAQKGDAT 251
Query: 708 NAEGGDQ 728
N GG +
Sbjct: 252 NKAGGGE 258
>sp|Q9C5W6|143C_ARATH 14-3-3-like protein GF14 iota (General regulatory factor 12)
Length = 268
Score = 290 bits (743), Expect = 2e-78
Identities = 151/244 (61%), Positives = 194/244 (79%), Gaps = 6/244 (2%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKL+EQAERYDEMV++MK VA ++EL+VEERNLLSV +KNVIG+RRASWRI+S+IEQ+E
Sbjct: 17 AKLSEQAERYDEMVETMKKVARVNSELTVEERNLLSVGYKNVIGARRASWRIMSSIEQKE 76
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E+KG+ + ++ K YR++VE ELA +C +++ ++D L+P A++ E+ VFY KMKGDYY
Sbjct: 77 ESKGN-ESNVKQIKGYRQKVEDELANICQDILTIIDQHLIPHATSGEATVFYYKMKGDYY 135
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF E+ER+E A++SL YE+A A + L +THPIRLGLALNFSVFY+EIM +PE
Sbjct: 136 RYLAEFKTEQERKEAAEQSLKGYEAATQAASTELPSTHPIRLGLALNFSVFYYEIMNSPE 195
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNS-----GEEDDNKQNE 704
+AC LAK AFD+AI ELD L+EE +YKDSTLIMQLLRDNLTLW S G ED+ K E
Sbjct: 196 RACHLAKQAFDEAIAELDTLSEE-SYKDSTLIMQLLRDNLTLWTSDLPEDGGEDNIKTEE 254
Query: 705 TNAE 716
+ E
Sbjct: 255 SKQE 258
>sp|Q96453|1433D_SOYBN 14-3-3-like protein D (SGF14D)
Length = 261
Score = 289 bits (740), Expect = 5e-78
Identities = 156/247 (63%), Positives = 190/247 (76%), Gaps = 5/247 (2%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKLAEQAERY+EMV+SMK VA D EL+VEERNLLSV +KNVIG+RRASWRILS+IEQ+E
Sbjct: 14 AKLAEQAERYEEMVESMKNVANLDVELTVEERNLLSVGYKNVIGARRASWRILSSIEQKE 73
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E KG +++ + K YR++VE EL+ +C++V++V+D L+P A+ ES VFY KMKGDYY
Sbjct: 74 ETKG-NELNAKRIKEYRQKVELELSNICNDVMRVIDEHLIPSAAAGESTVFYYKMKGDYY 132
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF E++E A +S+ AYESA AE+ L THPIRLGLALNFSVFY+EI+ +PE
Sbjct: 133 RYLAEFKSGNEKKEAADQSMKAYESATAAAEADLPPTHPIRLGLALNFSVFYYEILNSPE 192
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNS----GEEDDNKQNET 707
+AC LAK AFD+AI ELD L EE+YKDSTLIMQLLRDNLTLW S ED K N T
Sbjct: 193 RACHLAKQAFDEAISELDTL-NEESYKDSTLIMQLLRDNLTLWTSDIPEDGEDAQKVNGT 251
Query: 708 NAEGGDQ 728
GG +
Sbjct: 252 AKLGGGE 258
>sp|P48347|14310_ARATH 14-3-3-like protein GF14 epsilon (General regulatory factor 10)
Length = 254
Score = 289 bits (739), Expect = 7e-78
Identities = 149/243 (61%), Positives = 193/243 (79%), Gaps = 1/243 (0%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKL+EQ ERYDEMV++MK VA D EL+VEERNL+SV +KNVIG+RRASWRILS+IEQ+E
Sbjct: 12 AKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSVGYKNVIGARRASWRILSSIEQKE 71
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E+KG+ + ++ K YRK+VE ELA+VC++++ V+D L+P ++ ES VF+ KMKGDYY
Sbjct: 72 ESKGNDE-NVKRLKNYRKRVEDELAKVCNDILSVIDKHLIPSSNAVESTVFFYKMKGDYY 130
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF+ ER+E A +SL AY++A+ AE+ L+ THP+RLGLALNFSVFY+EI+ +PE
Sbjct: 131 RYLAEFSSGAERKEAADQSLEAYKAAVAAAENGLAPTHPVRLGLALNFSVFYYEILNSPE 190
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDDNKQNETNAEG 719
AC LAK AFD AI ELD+L EE+YKDSTLIMQLLRDNLTLW S D N++ + +G
Sbjct: 191 SACQLAKQAFDDAIAELDSL-NEESYKDSTLIMQLLRDNLTLWTS---DLNEEGDERTKG 246
Query: 720 GDQ 728
D+
Sbjct: 247 ADE 249
>sp|P93212|1437_LYCES 14-3-3 protein 7
Length = 252
Score = 288 bits (738), Expect = 9e-78
Identities = 146/242 (60%), Positives = 191/242 (78%), Gaps = 1/242 (0%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
A+LAEQAERYDEMV++MK +A D EL+VEERNL+SV +KNVIG+RRASWRILS+IEQ+E
Sbjct: 12 ARLAEQAERYDEMVEAMKAIAKMDVELTVEERNLVSVGYKNVIGARRASWRILSSIEQKE 71
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E+KG Q ++ K YR++VE EL ++C +++ V+D L+P ++ ES VFY KMKGDYY
Sbjct: 72 ESKGHEQ-NVKRIKTYRQRVEDELTKICSDILSVIDEHLVPSSTTGESTVFYYKMKGDYY 130
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF +R+E +++SL AYE+A A S L+ THPIRLGLALNFSVFY+EI+ +PE
Sbjct: 131 RYLAEFKAGDDRKEASEQSLKAYEAATATASSDLAPTHPIRLGLALNFSVFYYEILNSPE 190
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDDNKQNETNAEG 719
+AC LAK AFD+AI ELD+L+ EE+YKDSTLIMQLLRDNLTLW S E+ + ++ +
Sbjct: 191 RACHLAKQAFDEAIAELDSLS-EESYKDSTLIMQLLRDNLTLWTSDLEEGGEHSKGDERQ 249
Query: 720 GD 725
G+
Sbjct: 250 GE 251
>sp|Q99002|1433_TRIHA 14-3-3 protein homolog (Th1433)
Length = 262
Score = 288 bits (736), Expect = 2e-77
Identities = 147/229 (64%), Positives = 190/229 (82%), Gaps = 1/229 (0%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKLAEQAERY+EMV++MK VA+ D +L+VEERNLLSVA+KNVIG+RRASWRI+++IEQ+E
Sbjct: 10 AKLAEQAERYEEMVENMKIVASEDRDLTVEERNLLSVAYKNVIGARRASWRIVTSIEQKE 69
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E+KG+S ++ L K YR+++E ELA++CD++++VLD L+P A + ES+VFY K+KGDY+
Sbjct: 70 ESKGNSS-QVTLIKEYRQKIENELAKICDDILEVLDQHLIPSAKSGESKVFYHKIKGDYH 128
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEFAI R++ A KSL AY++A A++ L THPIRLGLALNFSVFY+EI+ P+
Sbjct: 129 RYLAEFAIGDRRKDSADKSLEAYKAATEVAQTELPPTHPIRLGLALNFSVFYYEILNAPD 188
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEED 686
+AC LAK AFD AI ELD L+ EE+YKDSTLIMQLLRDNLTLW S E +
Sbjct: 189 QACHLAKQAFDDAIAELDTLS-EESYKDSTLIMQLLRDNLTLWTSSEAE 236
>sp|P34730|BMH2_YEAST Protein BMH2
Length = 273
Score = 287 bits (735), Expect = 2e-77
Identities = 150/248 (60%), Positives = 191/248 (77%), Gaps = 6/248 (2%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKLAEQAERY+EMV++MK VA+S ELSVEERNLLSVA+KNVIG+RRASWRI+S+IEQ+E
Sbjct: 12 AKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIVSSIEQKE 71
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E+K S+ ++EL ++YR ++E EL ++ D+++ VLD+ L+P A+ ES+VFY KMKGDY+
Sbjct: 72 ESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSATTGESKVFYYKMKGDYH 131
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF+ RE+ SL AY++A A + L THPIRLGLALNFSVFY+EI +P+
Sbjct: 132 RYLAEFSSGDAREKATNSSLEAYKTASEIATTELPPTHPIRLGLALNFSVFYYEIQNSPD 191
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLW-----NSGEEDDNKQNE 704
KAC LAK AFD AI ELD L+ EE+YKDSTLIMQLLRDNLTLW SG+ED +Q +
Sbjct: 192 KACHLAKQAFDDAIAELDTLS-EESYKDSTLIMQLLRDNLTLWTSDISESGQEDQQQQQQ 250
Query: 705 TNAEGGDQ 728
+ Q
Sbjct: 251 QQQQQQQQ 258
>sp|P42650|14333_ENTHI 14-3-3 protein 3 (14-3-3-3)
Length = 236
Score = 287 bits (735), Expect = 2e-77
Identities = 147/230 (63%), Positives = 186/230 (80%), Gaps = 1/230 (0%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
+KLAEQ+ERYDEMV+ MK VAA +TELSVEERNLLSVA+KNVIGSRRASWRI++++EQ+E
Sbjct: 8 SKLAEQSERYDEMVQYMKQVAALNTELSVEERNLLSVAYKNVIGSRRASWRIITSLEQKE 67
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
+AKG+ + +E+ K YR ++E ELA+ CD+V+KV+ LLP AS +ES+VFY KM+GDYY
Sbjct: 68 QAKGNDK-HVEIIKGYRAKIEDELAKYCDDVLKVIKENLLPNASTSESKVFYKKMEGDYY 126
Query: 363 RYRAEFAIEKEREEVAQKSLGAY-ESAMTEAESLSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF ++++R+EVA KSL AY E+ L+ THPIRLGLALNFSVFY+EIM + +
Sbjct: 127 RYYAEFTVDEKRQEVADKSLAAYTEATEISNADLAPTHPIRLGLALNFSVFYYEIMNDAD 186
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDD 689
KAC LAK AFD +I +LD + E +YKDSTLIMQLLRDNLTLW S D+
Sbjct: 187 KACQLAKQAFDDSIAKLDEV-PESSYKDSTLIMQLLRDNLTLWTSDTADE 235
>sp|P42654|1433B_VICFA 14-3-3-like protein B (VFA-1433B)
Length = 261
Score = 287 bits (734), Expect = 3e-77
Identities = 155/245 (63%), Positives = 186/245 (75%), Gaps = 5/245 (2%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKLAEQAERY+EMV SMK VA D EL++EERNLLSV +KNVIG+RRASWRILS+IEQ+E
Sbjct: 14 AKLAEQAERYEEMVDSMKNVANLDVELTIEERNLLSVGYKNVIGARRASWRILSSIEQKE 73
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E+KG + + + K YR +VE EL+ +C +V++V+D L+P A+ ES VFY KMKGDYY
Sbjct: 74 ESKG-NDVNAKRIKEYRHKVETELSNICIDVMRVIDEHLIPSAAAGESTVFYYKMKGDYY 132
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEAES-LSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEF E++E +S+ AYESA T AE+ L THPIRLGLALNFSVFY+EI+ +PE
Sbjct: 133 RYLAEFKTGNEKKEAGDQSMKAYESATTAAEAELPPTHPIRLGLALNFSVFYYEILNSPE 192
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNS----GEEDDNKQNET 707
+AC LAK AFD+AI ELD L EE+YKDSTLIMQLLRDNLTLW S ED K N T
Sbjct: 193 RACHLAKQAFDEAISELDTL-NEESYKDSTLIMQLLRDNLTLWTSDIPEDGEDSQKANGT 251
Query: 708 NAEGG 722
GG
Sbjct: 252 AKFGG 256
>sp|P92177|1433E_DROME 14-3-3 protein epsilon (Suppressor of Ras1 3-9)
Length = 262
Score = 287 bits (734), Expect = 3e-77
Identities = 150/242 (61%), Positives = 191/242 (78%), Gaps = 1/242 (0%)
Frame = +3
Query: 3 AKLAEQAERYDEMVKSMKCVAASDTELSVEERNLLSVAFKNVIGSRRASWRILSTIEQRE 182
AKLAEQAERYDEMV++MK VA+ D EL+VEERNLLSVA+KNVIG+RRASWRI+++IEQ+E
Sbjct: 11 AKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYKNVIGARRASWRIITSIEQKE 70
Query: 183 EAKGSSQMRIELAKAYRKQVEKELAEVCDEVVKVLDTQLLPKASNTESQVFYLKMKGDYY 362
E KG+ + ++E+ K YR QVEKEL ++C +++ VL+ L+P A++ ES+VFY KMKGDY+
Sbjct: 71 ENKGAEE-KLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKVFYYKMKGDYH 129
Query: 363 RYRAEFAIEKEREEVAQKSLGAYESAMTEA-ESLSATHPIRLGLALNFSVFYFEIMGNPE 539
RY AEFA +R++ A+ SL AY++A A L THPIRLGLALNFSVFY+EI+ +P+
Sbjct: 130 RYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSVFYYEILNSPD 189
Query: 540 KACSLAKTAFDQAIGELDNLTEEENYKDSTLIMQLLRDNLTLWNSGEEDDNKQNETNAEG 719
+AC LAK AFD AI ELD L+ EE+YKDSTLIMQLLRDNLTLW S D + E +
Sbjct: 190 RACRLAKAAFDDAIAELDTLS-EESYKDSTLIMQLLRDNLTLWTS----DMQAEEVDPNA 244
Query: 720 GD 725
GD
Sbjct: 245 GD 246
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,329,043
Number of Sequences: 369166
Number of extensions: 1413449
Number of successful extensions: 4884
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4531
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7666799535
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)