Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_N13
(797 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 131 2e-30
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 130 4e-30
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 126 8e-29
sp|P27636|CDC15_YEAST Cell division control protein 15 125 2e-28
sp|P41892|CDC7_SCHPO Cell division control protein 7 118 2e-26
sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 117 3e-26
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 117 5e-26
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 117 5e-26
sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinas... 116 6e-26
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 116 8e-26
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 131 bits (329), Expect = 2e-30
Identities = 82/240 (34%), Positives = 126/240 (52%), Gaps = 1/240 (0%)
Frame = +3
Query: 27 KDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMAN 206
K +++ + +++L E + + L HP I++ G R + I L+++
Sbjct: 97 KQVLITSNCASKEKTQAHIQELEEEVKLLKNLS-HPNIVRYLGTVREDETLNILLEFVPG 155
Query: 207 GSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF 386
GS+ S L K GA E + ++ Q+L L++LH+ I+HRD+KG IL+DN IKL F
Sbjct: 156 GSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADF 215
Query: 387 MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKA 566
++K + ++ + A+S GT W APEVI G+ SADIWS G ++E++TGKA
Sbjct: 216 GASKQVAELATIS----GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKA 271
Query: 567 PLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 743
P AI+ +GT K P S F+ CL EP RPT +L+KH F+
Sbjct: 272 PWSQQYKEIAAIFHIGTTKS-HPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 130 bits (327), Expect = 4e-30
Identities = 83/240 (34%), Positives = 127/240 (52%), Gaps = 1/240 (0%)
Frame = +3
Query: 27 KDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMAN 206
K +++ + + +++L E + + L HP I++ G R I L+++
Sbjct: 98 KQVLIAANFASKEKTQAHIQELEEEVKLLKNLS-HPNIVRYLGTVREDDTLNILLEFVPG 156
Query: 207 GSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF 386
GS+ S L K G E + +++Q+L L++LH+ I+HRD+KG IL+DN IKL F
Sbjct: 157 GSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADF 216
Query: 387 MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKA 566
++K + ++ T A+S GT W APEVI G+ SADIWS G ++E++TGKA
Sbjct: 217 GASKQVAELATMT----GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKA 272
Query: 567 PLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 743
P AI+ +GT K SDA K F+ CL P RPT +L+KH F+
Sbjct: 273 PWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDA-KDFLLKCLQEVPNLRPTASELLKHPFV 331
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 126 bits (316), Expect = 8e-29
Identities = 79/240 (32%), Positives = 127/240 (52%), Gaps = 1/240 (0%)
Frame = +3
Query: 27 KDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMAN 206
K +++ + +++L E + + L HP I++ G R + I ++++
Sbjct: 97 KQVLIAPSSASKEKTQGHIRELEEEVQLLKNLS-HPNIVRYLGTVRESDSLNILMEFVPG 155
Query: 207 GSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF 386
GS+ S L K G+ EP II ++KQ+L L++LH+ I+HRD+KG IL+DN I+L F
Sbjct: 156 GSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADF 215
Query: 387 MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKA 566
++K + ++ + A+S GT W APEVI G+ SADIWS G ++E+ TGK
Sbjct: 216 GASKKVVELATV----NGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKP 271
Query: 567 PLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 743
P F A+ +G + K P S K F+ CL EP R + +L++H F+
Sbjct: 272 PWSEQYQQFAAVLHIG-RTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 125 bits (313), Expect = 2e-28
Identities = 77/229 (33%), Positives = 112/229 (48%), Gaps = 1/229 (0%)
Frame = +3
Query: 66 DKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQ 245
+ DEEL +M E S+ + L +H I+K +GF R YI L+Y ANGSLR + ++
Sbjct: 59 ENDEELNDIMAEISLLKNL-NHNNIVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTG 117
Query: 246 -KEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSE 422
E + Q L L +LH + ++HRD+K ILL + +KL F
Sbjct: 118 LSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANILLSADNTVKLADF---------GVS 168
Query: 423 TFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIY 602
T N A + GT NW APE++ G + +DIWS GA +VE++T P L Y
Sbjct: 169 TIVNSSALTLAGTLNWMAPEILGNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYY 228
Query: 603 RLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNS 749
+ + + P S+ LK F+ C RPT L+KH ++NS
Sbjct: 229 AV---ENDTYYPPSSFSEPLKDFLSKCFVKNMYKRPTADQLLKHVWINS 274
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 118 bits (295), Expect = 2e-26
Identities = 81/261 (31%), Positives = 127/261 (48%)
Frame = +3
Query: 3 YRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASY 182
YR + + V +KK K +L + +E + + L DHP I+K G +++ +
Sbjct: 24 YRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLKNL-DHPNIVKYRGSYQTNDSLC 82
Query: 183 IKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNN 362
I L+Y NGSLRS G E + ++ Q+L+ L +LH+Q ++HRD+KG IL +
Sbjct: 83 IILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLHNQGVIHRDIKGANILTTKD 142
Query: 363 LDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVM 542
IKL F A +H S G+ W APEVI G +++DIWS G +
Sbjct: 143 GTIKLADFGVA-----TKINALEDH---SVVGSPYWMAPEVIELVGATTASDIWSVGCTV 194
Query: 543 VELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKD 722
+EL+ G P + L A++R+ K P S A KSF+ C +P R +
Sbjct: 195 IELLDGNPPYYDLDPTSALFRM--VKDEHPPLPSNISSAAKSFLMQCFQKDPNLRIKTRK 252
Query: 723 LMKHEFLNSAMEAD*FT*RVD 785
L+KH ++ + F+ +D
Sbjct: 253 LLKHPWVIMNQTSSKFSDAID 273
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
Length = 1607
Score = 117 bits (294), Expect = 3e-26
Identities = 88/254 (34%), Positives = 139/254 (54%), Gaps = 7/254 (2%)
Frame = +3
Query: 3 YRCSKFGSKDMVVLKKYK-NPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKAS 179
Y C + +++ +K+ + PND + +K+ E I+E + HP +++ G +
Sbjct: 1357 YTCISVDTGELMAMKEIRFQPNDH-KTIKETADELKIFEGI-KHPNLVRYFGVELHREEM 1414
Query: 180 YIKLDYMANGSLRSWLHKNGAQKEPSIIR-FSKQILEALDFLHSQNILHRDLKGPKILLD 356
YI ++Y G+L + + G Q+ +IR +SKQI A++ LH I+HRD+KG I L
Sbjct: 1415 YIFMEYCDEGTLEE-VSRLGLQEH--VIRLYSKQITIAINVLHEHGIVHRDIKGANIFLT 1471
Query: 357 NNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN---GEGYGSSADIW 524
++ IKL F S K+ +A +T V S GTA + APEVI GEG+G +ADIW
Sbjct: 1472 SSGLIKLGDFGCSVKLKNNA--QTMPGEV-NSTLGTAAYMAPEVITRAKGEGHGRAADIW 1528
Query: 525 SFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPL 701
S G V++E++TGK P + +FQ +Y++G K P + S K F+ CL+ +P
Sbjct: 1529 SLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PERLSPEGKDFLSHCLESDPK 1586
Query: 702 NRPTVKDLMKHEFL 743
R T L+ H F+
Sbjct: 1587 MRWTASQLLDHSFV 1600
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 117 bits (292), Expect = 5e-26
Identities = 77/238 (32%), Positives = 118/238 (49%), Gaps = 2/238 (0%)
Frame = +3
Query: 36 VVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMANGSL 215
VV K + + ++E++ + E ++ + D P + K G + +I ++Y+ GS
Sbjct: 61 VVAIKIIDLEEAEDEIEDIQQEITVLSQC-DSPYVTKYYGSYLKDTKLWIIMEYLGGGSA 119
Query: 216 RSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSA 395
L G E I ++IL+ LD+LHS+ +HRD+K +LL + ++KL F A
Sbjct: 120 LDLLEP-GPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVA 178
Query: 396 KMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF 575
D + RN GT W APEVI Y S ADIWS G +EL G+ P
Sbjct: 179 GQLTDTQIK--RNTFV----GTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHS 232
Query: 576 FLASFQAIYRL--GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 743
L + ++ + +E Y S LK FV+ CL+ EP RPT K+L+KH+F+
Sbjct: 233 ELHPMKVLFLIPKNNPPTLEGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 286
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 117 bits (292), Expect = 5e-26
Identities = 78/257 (30%), Positives = 127/257 (49%), Gaps = 11/257 (4%)
Frame = +3
Query: 3 YRCSKFGSKDMVVLKKYKNPNDKDEE------LKKLMVEFSIYEKLGDHPRILKCNGFFR 164
Y C + +M+ +K+ + P + ++ L E S + L DH I++ GF
Sbjct: 1190 YLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKDL-DHLNIVQYLGFEN 1248
Query: 165 SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPK 344
+ L+Y+A GS+ S + G EP I + Q+L+ L +LHS+ ILHRD+K
Sbjct: 1249 KNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLHSKGILHRDMKADN 1308
Query: 345 ILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN-GEGYGSSADI 521
+LLD + K+ F ++ D S + + GT W APE+++ +GY + DI
Sbjct: 1309 LLLDQDGICKISDFGISRKSKDIYSNS-----DMTMRGTVFWMAPEMVDTKQGYSAKVDI 1363
Query: 522 WSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKI----EFAYPLKCSDALKSFVDPCLD 689
WS G +++E+ GK P L A++++G K E PL S ++F+D C +
Sbjct: 1364 WSLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPL-ISQIGRNFLDACFE 1422
Query: 690 PEPLNRPTVKDLMKHEF 740
P RPT +L+ H F
Sbjct: 1423 INPEKRPTANELLSHPF 1439
>sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4)
Length = 1597
Score = 116 bits (291), Expect = 6e-26
Identities = 87/254 (34%), Positives = 140/254 (55%), Gaps = 7/254 (2%)
Frame = +3
Query: 3 YRCSKFGSKDMVVLKKYK-NPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKAS 179
Y C + +++ +K+ + PND + +K+ E I+E + HP +++ G +
Sbjct: 1347 YTCISVDTGELMAMKEIRFQPNDH-KTIKETADELKIFEGI-KHPNLVRYFGVELHREEM 1404
Query: 180 YIKLDYMANGSLRSWLHKNGAQKEPSIIR-FSKQILEALDFLHSQNILHRDLKGPKILLD 356
YI ++Y G+L + + G Q+ +IR ++KQI A++ LH I+HRD+KG I L
Sbjct: 1405 YIFMEYCDEGTLEE-VSRLGLQEH--VIRLYTKQITVAINVLHEHGIVHRDIKGANIFLT 1461
Query: 357 NNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN---GEGYGSSADIW 524
++ IKL F S K+ +A +T V S GTA + APEVI GEG+G +ADIW
Sbjct: 1462 SSGLIKLGDFGCSVKLKNNA--QTMPGEV-NSTLGTAAYMAPEVITRAKGEGHGRAADIW 1518
Query: 525 SFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPL 701
S G V++E++TGK P + +FQ +Y++G K P + S K+F+ CL+ +P
Sbjct: 1519 SLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PERLSPEGKAFLSHCLESDPK 1576
Query: 702 NRPTVKDLMKHEFL 743
R T L+ H F+
Sbjct: 1577 IRWTASQLLDHAFV 1590
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 116 bits (290), Expect = 8e-26
Identities = 77/238 (32%), Positives = 118/238 (49%), Gaps = 2/238 (0%)
Frame = +3
Query: 36 VVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMANGSL 215
VV K + + ++E++ + E ++ + D P + K G + +I ++Y+ GS
Sbjct: 49 VVAIKIIDLEEAEDEIEDIQQEITVLSQC-DSPYVTKYYGSYLKDTKLWIIMEYLGGGSA 107
Query: 216 RSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSA 395
L G E I ++IL+ LD+LHS+ +HRD+K +LL + ++KL F A
Sbjct: 108 LDLLEP-GPLDEIQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVA 166
Query: 396 KMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF 575
D + RN GT W APEVI Y S ADIWS G +EL G+ P
Sbjct: 167 GQLTDTQIK--RNTFV----GTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGEPPHS 220
Query: 576 FLASFQAIYRL--GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 743
L + ++ + +E Y S LK FV+ CL+ EP RPT K+L+KH+F+
Sbjct: 221 ELHPMKVLFLIPKNNPPTLEGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 274
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,577,644
Number of Sequences: 369166
Number of extensions: 2085106
Number of successful extensions: 8779
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6752
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7173
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7522142940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)