Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_N01
(553 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8NCC3|LYPA3_HUMAN 1-O-acylceramide synthase precursor (... 82 7e-16
sp|Q8VEB4|LYPA3_MOUSE 1-O-acylceramide synthase precursor (... 80 3e-15
sp|P18424|LCAT_RAT Phosphatidylcholine-sterol acyltransfera... 79 6e-15
sp|P16301|LCAT_MOUSE Phosphatidylcholine-sterol acyltransfe... 78 1e-14
sp|P53760|LCAT_CHICK Phosphatidylcholine-sterol acyltransfe... 69 1e-11
sp|P04180|LCAT_HUMAN Phosphatidylcholine-sterol acyltransfe... 68 2e-11
sp|O35724|LCAT_MICMN Phosphatidylcholine-sterol acyltransfe... 68 2e-11
sp|Q08758|LCAT_PAPAN Phosphatidylcholine-sterol acyltransfe... 67 2e-11
sp|O35840|LCAT_TATKG Phosphatidylcholine-sterol acyltransfe... 66 6e-11
sp|P53761|LCAT_RABIT Phosphatidylcholine-sterol acyltransfe... 62 7e-10
>sp|Q8NCC3|LYPA3_HUMAN 1-O-acylceramide synthase precursor (ACS) (Lysosomal phospholipase
A2) (Lysophospholipase 3) (LPLA2) (LCAT-like
lysophospholipase) (LLPL)
Length = 412
Score = 82.4 bits (202), Expect = 7e-16
Identities = 60/189 (31%), Positives = 98/189 (51%), Gaps = 12/189 (6%)
Frame = +3
Query: 21 HLMAIIKGLLIFQIICIVGNLQICSSKVLNDSKIPSPIILVPGDGGSRAYSMVD-----Y 185
HL GLL ++ ++ L + + L + P P++LVPGD G++ + +D +
Sbjct: 4 HLRPYRVGLLPDGLLFLLLLLMLLADPALPAGRHP-PVVLVPGDLGNQLEAKLDKPTVVH 62
Query: 186 RLFTTKV----LVWVNFEYML---FPSFLRNVFQLEYNPDTHNTSNMKGSDVFFPGWGDT 344
L + K +W+N E +L ++ N+ +L YN + T G DV PG+G T
Sbjct: 63 YLCSKKTESYFTIWLNLELLLPVIIDCWIDNI-RLVYNKTSRATQFPDGVDVRVPGFGKT 121
Query: 345 DTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTVRGAPYDFRKAPNENQEMIQKMKLL 524
++E LD +YF +V +LV Y R + VRGAPYD+R+APNEN ++ +
Sbjct: 122 FSLEFLDPSKSSVGSYFHTMVESLV-GWGYTRGEDVRGAPYDWRRAPNENGPYFLALREM 180
Query: 525 VEDTYRQSG 551
+E+ Y+ G
Sbjct: 181 IEEMYQLYG 189
>sp|Q8VEB4|LYPA3_MOUSE 1-O-acylceramide synthase precursor (ACS) (Lysosomal phospholipase
A2) (Lysophospholipase 3) (LCAT-like lysophospholipase)
(LLPL)
Length = 412
Score = 80.1 bits (196), Expect = 3e-15
Identities = 55/166 (33%), Positives = 90/166 (54%), Gaps = 16/166 (9%)
Frame = +3
Query: 102 VLNDSKIPS----PIILVPGDGGSRAYSMVD-----YRLFTTKV----LVWVNFEYML-- 236
+L D +P+ P++LVPGD G++ + +D + L + K +W+N E +L
Sbjct: 26 MLADLTLPAQRHPPVVLVPGDLGNQLEAKLDKPKVVHYLCSKKTDSYFTLWLNLELLLPV 85
Query: 237 -FPSFLRNVFQLEYNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNN 413
++ N+ +L YN + T G DV PG+G+T ++E LD +YF +V +
Sbjct: 86 IIDCWIDNI-RLVYNRTSRATQFPDGVDVRVPGFGETFSMEFLDPSKRNVGSYFYTMVES 144
Query: 414 LVTDKFYVRNKTVRGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
LV Y R + VRGAPYD+R+APNEN ++ ++E+ Y+ G
Sbjct: 145 LV-GWGYTRGEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQMYG 189
>sp|P18424|LCAT_RAT Phosphatidylcholine-sterol acyltransferase precursor
(Lecithin-cholesterol acyltransferase)
(Phospholipid-cholesterol acyltransferase)
Length = 440
Score = 79.3 bits (194), Expect = 6e-15
Identities = 54/153 (35%), Positives = 80/153 (52%), Gaps = 12/153 (7%)
Frame = +3
Query: 129 PIILVPGDGGSRAYSMVD---------YRLFTTKVLVWVNFEYML---FPSFLRNVFQLE 272
P+ILVPG G+R + +D YR +W++F L ++ N ++
Sbjct: 48 PVILVPGCMGNRLEAKLDKPNVVNWLCYRKTEDFFTIWLDFNMFLPLGVDCWIDNT-RVV 106
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN + + SN G + PG+G T ++E LD+ Y + LV NLV + YVR++TV
Sbjct: 107 YNRSSGHMSNAPGVQIRVPGFGKTYSVEYLDDNK--LAGYLNTLVQNLVNNG-YVRDETV 163
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
R APYD+R AP + E QK+ LVE+ Y G
Sbjct: 164 RAAPYDWRLAPRQQDEYYQKLAGLVEEMYAAYG 196
>sp|P16301|LCAT_MOUSE Phosphatidylcholine-sterol acyltransferase precursor
(Lecithin-cholesterol acyltransferase)
(Phospholipid-cholesterol acyltransferase)
Length = 438
Score = 78.2 bits (191), Expect = 1e-14
Identities = 52/153 (33%), Positives = 80/153 (52%), Gaps = 12/153 (7%)
Frame = +3
Query: 129 PIILVPGDGGSRAYSMVD---------YRLFTTKVLVWVNFEYML---FPSFLRNVFQLE 272
P+ILVPG G+R + +D YR +W++F L ++ N ++
Sbjct: 48 PVILVPGCLGNRLEAKLDKPDVVNWMCYRKTEDFFTIWLDFNLFLPLGVDCWIDNT-RIV 106
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN + SN G + PG+G T+++E +D+ Y LV NLV + YVR++TV
Sbjct: 107 YNHSSGRVSNAPGVQIRVPGFGKTESVEYVDDNK--LAGYLHTLVQNLVNNG-YVRDETV 163
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
R APYD+R AP++ E +K+ LVE+ Y G
Sbjct: 164 RAAPYDWRLAPHQQDEYYKKLAGLVEEMYAAYG 196
>sp|P53760|LCAT_CHICK Phosphatidylcholine-sterol acyltransferase precursor
(Lecithin-cholesterol acyltransferase)
(Phospholipid-cholesterol acyltransferase)
Length = 413
Score = 68.6 bits (166), Expect = 1e-11
Identities = 51/154 (33%), Positives = 74/154 (48%), Gaps = 15/154 (9%)
Frame = +3
Query: 129 PIILVPGDGGSRAYSMVD---------YRLFTTKVLVWVNFEYML---FPSFLRNVFQLE 272
P LVPG G++ + +D YR +W+N L ++ N ++
Sbjct: 46 PWCLVPGFLGNQLEAKLDKPDVVNWMCYRKTEDYFTIWLNLNTFLPVGVDCWIDNT-RVV 104
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN +N G + PG+G T ++E LD+ Y LV NLV + YVR++TV
Sbjct: 105 YNRTARKMTNAPGVHIRVPGFGKTYSVEYLDQSK--LAGYLHTLVQNLVNNG-YVRDQTV 161
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVE---DTYRQ 545
R APYD+R P E E Q +K L+E D Y+Q
Sbjct: 162 RAAPYDWRVGPQEQPEYFQNLKALIEEMHDEYQQ 195
>sp|P04180|LCAT_HUMAN Phosphatidylcholine-sterol acyltransferase precursor
(Lecithin-cholesterol acyltransferase)
(Phospholipid-cholesterol acyltransferase)
Length = 440
Score = 67.8 bits (164), Expect = 2e-11
Identities = 49/153 (32%), Positives = 76/153 (49%), Gaps = 12/153 (7%)
Frame = +3
Query: 129 PIILVPGDGGSRAYSMVD---------YRLFTTKVLVWVNFEYML---FPSFLRNVFQLE 272
P+ILVPG G++ + +D YR +W++ L ++ N ++
Sbjct: 48 PVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNT-RVV 106
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN + SN G + PG+G T ++E LD Y LV NLV + YVR++TV
Sbjct: 107 YNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSK--LAGYLHTLVQNLVNNG-YVRDETV 163
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
R APYD+R P + +E +K+ LVE+ + G
Sbjct: 164 RAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYG 196
>sp|O35724|LCAT_MICMN Phosphatidylcholine-sterol acyltransferase (Lecithin-cholesterol
acyltransferase) (Phospholipid-cholesterol
acyltransferase)
Length = 299
Score = 67.8 bits (164), Expect = 2e-11
Identities = 39/93 (41%), Positives = 53/93 (56%)
Frame = +3
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN + SN G + PG+G T ++E LD+ Y LV NLV + YVR++TV
Sbjct: 27 YNRSSGRMSNAPGVQIRVPGFGKTYSVEYLDDNK--LAGYLHTLVQNLVNNA-YVRDETV 83
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
R APYD+R AP++ E QK+ LVE+ Y G
Sbjct: 84 RAAPYDWRLAPSQQDEYYQKLAELVEEMYDAYG 116
>sp|Q08758|LCAT_PAPAN Phosphatidylcholine-sterol acyltransferase precursor
(Lecithin-cholesterol acyltransferase)
(Phospholipid-cholesterol acyltransferase)
Length = 440
Score = 67.4 bits (163), Expect = 2e-11
Identities = 49/153 (32%), Positives = 75/153 (49%), Gaps = 12/153 (7%)
Frame = +3
Query: 129 PIILVPGDGGSRAYSMVD---------YRLFTTKVLVWVNFEYML---FPSFLRNVFQLE 272
P+ILVPG G++ + +D YR +W++ L ++ N ++
Sbjct: 48 PVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNT-RVV 106
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN + SN G + PG+G T ++E LD Y LV NLV + YVR++TV
Sbjct: 107 YNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSK--LAGYLHTLVQNLVNNG-YVRDETV 163
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
R APYD+R P + +E K+ LVE+ + G
Sbjct: 164 RAAPYDWRLEPGQQEEYYHKLAGLVEEMHAAYG 196
>sp|O35840|LCAT_TATKG Phosphatidylcholine-sterol acyltransferase (Lecithin-cholesterol
acyltransferase) (Phospholipid-cholesterol
acyltransferase)
Length = 293
Score = 65.9 bits (159), Expect = 6e-11
Identities = 36/93 (38%), Positives = 54/93 (58%)
Frame = +3
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN + SN G ++ PG+G T ++E LD+ + Y LV NLV + YVR++TV
Sbjct: 25 YNRSSGRVSNAPGVEIRVPGFGKTYSVEYLDDNKLAE--YMHTLVQNLVNNG-YVRDETV 81
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
R APYD+R P++ + QK+ L+E+ Y G
Sbjct: 82 RAAPYDWRLEPSQQDDYYQKLAGLIEEMYAAYG 114
>sp|P53761|LCAT_RABIT Phosphatidylcholine-sterol acyltransferase precursor
(Lecithin-cholesterol acyltransferase)
(Phospholipid-cholesterol acyltransferase)
Length = 440
Score = 62.4 bits (150), Expect = 7e-10
Identities = 47/153 (30%), Positives = 74/153 (48%), Gaps = 12/153 (7%)
Frame = +3
Query: 129 PIILVPGDGGSRAYSMVD---------YRLFTTKVLVWVNFEYML---FPSFLRNVFQLE 272
P+ILVPG G++ + +D YR +W++ L ++ N ++
Sbjct: 48 PVILVPGCLGNQLEAKLDKPSVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNT-RVV 106
Query: 273 YNPDTHNTSNMKGSDVFFPGWGDTDTIEELDEKLHIQTTYFSNLVNNLVTDKFYVRNKTV 452
YN + G + PG+G T ++E LD Y LV NLV + YVR++TV
Sbjct: 107 YNRSSGRVVISPGVQIRVPGFGKTYSVEYLDNNK--LAGYMHTLVQNLVNNG-YVRDETV 163
Query: 453 RGAPYDFRKAPNENQEMIQKMKLLVEDTYRQSG 551
R APYD+R P++ +E K+ LVE+ + G
Sbjct: 164 RAAPYDWRLEPSQQEEYYGKLAGLVEEMHAAYG 196
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,165,207
Number of Sequences: 369166
Number of extensions: 1407488
Number of successful extensions: 3477
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3395
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3464
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3882260660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)