Planarian EST Database


Dr_sW_025_M04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_025_M04
         (442 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P31395|STMN1_CHICK  Stathmin                                    34   0.12 
sp|Q89YY3|CLPB_BACTN  Chaperone clpB                               34   0.16 
sp|P16949|STMN1_HUMAN  Stathmin (Phosphoprotein p19) (pp19) ...    33   0.27 
sp|P13668|STMN1_RAT  Stathmin (Phosphoprotein p19) (pp19) (O...    33   0.27 
sp|Q28298|RRBP1_CANFA  Ribosome-binding protein 1 (180 kDa r...    33   0.36 
sp|Q8D2B6|Y438_WIGBR  Hypothetical UPF0350 protein WIGBR4380       33   0.36 
sp|P54227|STMN1_MOUSE  Stathmin (Phosphoprotein p19) (pp19) ...    32   0.61 
sp|P93484|VSR1_PEA  Vacuolar sorting receptor 1 precursor (B...    32   0.61 
sp|P34278|YKK1_CAEEL  Hypothetical protein C02F5.1 in chromo...    32   0.80 
sp|O14617|AP3D1_HUMAN  Adapter-related protein complex 3 del...    32   0.80 
>sp|P31395|STMN1_CHICK Stathmin
          Length = 148

 Score = 34.3 bits (77), Expect = 0.12
 Identities = 32/108 (29%), Positives = 53/108 (49%), Gaps = 6/108 (5%)
 Frame = +1

Query: 40  EYKFDLSRKGDDKCEELNGKID--EECEDPYEA-LDKECDPKYEVLDKECLPKYVEVNAN 210
           E+     +K D   EE+  K++  EE    +EA + K+   K E  +KE L K +E N N
Sbjct: 34  EFPLSPPKKKDLSLEEIQKKLEAAEERRKSHEAEVLKQLAEKREH-EKEVLQKAIEENNN 92

Query: 211 FAKSVVDAKV-KCENNQSKKSRQVVRNLEKIIFGD--YDKIDKTCDGK 345
           F+K   +    K E N+  +  Q+   LE++   D   +++ K  +GK
Sbjct: 93  FSKMAEEKLTHKMEANKENREAQMAAKLERLREKDKHIEEVRKNKEGK 140
>sp|Q89YY3|CLPB_BACTN Chaperone clpB
          Length = 862

 Score = 33.9 bits (76), Expect = 0.16
 Identities = 35/115 (30%), Positives = 54/115 (46%), Gaps = 9/115 (7%)
 Frame = +1

Query: 73  DKCEELNGKIDEECEDPYEALDKECD-PKYEVLDKECLPKYVEVNANFAKSVVDAKVKCE 249
           ++ +E++ KI ++ E   EA+ +E D PK E++ KE L +  EV  +F      AK + E
Sbjct: 408 EELDEISRKI-KQLEIEREAIKRENDKPKLEIIGKE-LAELKEVEKSFK-----AKWQSE 460

Query: 250 NNQSKKSRQVVRNLEKIIF--------GDYDKIDKTCDGKYAELDKSCLKRYEDL 390
                K +Q    +E + F        GDY K+ +   GK   LDK      E L
Sbjct: 461 KTLMDKIQQNKVEIENLKFEAEKAEREGDYGKVAEIRYGKLQALDKEIEDTQEKL 515
>sp|P16949|STMN1_HUMAN Stathmin (Phosphoprotein p19) (pp19) (Oncoprotein 18) (Op18)
           (Leukemia-associated phosphoprotein p18) (pp17)
           (Prosolin) (Metablastin) (Pr22 protein)
          Length = 149

 Score = 33.1 bits (74), Expect = 0.27
 Identities = 28/91 (30%), Positives = 45/91 (49%), Gaps = 4/91 (4%)
 Frame = +1

Query: 40  EYKFDLSRKGDDKCEELNGKID--EECEDPYEA-LDKECDPKYEVLDKECLPKYVEVNAN 210
           E+     +K D   EE+  K++  EE    +EA + K+   K E  +KE L K +E N N
Sbjct: 34  EFPLSPPKKKDLSLEEIQKKLEAAEERRKSHEAEVLKQLAEKREH-EKEVLQKAIEENNN 92

Query: 211 FAKSVVDAKV-KCENNQSKKSRQVVRNLEKI 300
           F+K   +    K E N+  +  Q+   LE++
Sbjct: 93  FSKMAEEKLTHKMEANKENREAQMAAKLERL 123
>sp|P13668|STMN1_RAT Stathmin (Phosphoprotein p19) (pp19) (Oncoprotein 18) (Op18)
           (Leukemia-associated phosphoprotein p18) (pp17)
           (Prosolin) (Metablastin) (Pr22 protein)
          Length = 149

 Score = 33.1 bits (74), Expect = 0.27
 Identities = 28/91 (30%), Positives = 45/91 (49%), Gaps = 4/91 (4%)
 Frame = +1

Query: 40  EYKFDLSRKGDDKCEELNGKID--EECEDPYEA-LDKECDPKYEVLDKECLPKYVEVNAN 210
           E+     +K D   EE+  K++  EE    +EA + K+   K E  +KE L K +E N N
Sbjct: 34  EFPLSPPKKKDLSLEEIQKKLEAAEERRKSHEAEVLKQLAEKREH-EKEVLQKAIEENNN 92

Query: 211 FAKSVVDAKV-KCENNQSKKSRQVVRNLEKI 300
           F+K   +    K E N+  +  Q+   LE++
Sbjct: 93  FSKMAEEKLTHKMEANKENREAQMAAKLERL 123
>sp|Q28298|RRBP1_CANFA Ribosome-binding protein 1 (180 kDa ribosome receptor) (RRp)
          Length = 1534

 Score = 32.7 bits (73), Expect = 0.36
 Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 1/78 (1%)
 Frame = +1

Query: 73   DKCEELNGKIDEECEDPYEALDKECDPKYEVL-DKECLPKYVEVNANFAKSVVDAKVKCE 249
            D   +L  K+  E E+    L  ECD    +L + E + K ++ +    + V  AKV   
Sbjct: 1234 DPSSDLASKL-REAEETQNNLQAECDQYRTILAETEGMLKDLQKSVEEEEQVWKAKVSAT 1292

Query: 250  NNQSKKSRQVVRNLEKII 303
              + +KSR  V++LE I+
Sbjct: 1293 EEELQKSRVTVKHLEDIV 1310
>sp|Q8D2B6|Y438_WIGBR Hypothetical UPF0350 protein WIGBR4380
          Length = 84

 Score = 32.7 bits (73), Expect = 0.36
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = -1

Query: 355 ILHIFHHKFYRFYHNHQKLFFPNFLQLDDFFYFDC 251
           I++ F  K + F  N +K+FF N L  DD + + C
Sbjct: 23  IINNFFKKEFDFLSNKEKIFFVNMLSYDDIYLYKC 57
>sp|P54227|STMN1_MOUSE Stathmin (Phosphoprotein p19) (pp19) (Oncoprotein 18) (Op18)
           (Leukemia-associated phosphoprotein p18) (pp17)
           (Prosolin) (Metablastin) (Pr22 protein)
           (Leukemia-associated gene protein)
          Length = 149

 Score = 32.0 bits (71), Expect = 0.61
 Identities = 27/84 (32%), Positives = 43/84 (51%), Gaps = 4/84 (4%)
 Frame = +1

Query: 61  RKGDDKCEELNGKID--EECEDPYEA-LDKECDPKYEVLDKECLPKYVEVNANFAKSVVD 231
           +K D   EE+  K++  EE    +EA + K+   K E  +KE L K +E N NF+K   +
Sbjct: 41  KKKDLSLEEIQKKLEAAEERRKSHEAEVLKQLAEKREH-EKEVLQKAIEENNNFSKMAEE 99

Query: 232 AKV-KCENNQSKKSRQVVRNLEKI 300
               K E N+  +  Q+   LE++
Sbjct: 100 KLTHKMEANKENREAQMAAKLERL 123
>sp|P93484|VSR1_PEA Vacuolar sorting receptor 1 precursor (BP-80) (80 kDa proaleurein
           binding protein)
          Length = 623

 Score = 32.0 bits (71), Expect = 0.61
 Identities = 15/55 (27%), Positives = 29/55 (52%)
 Frame = +1

Query: 223 VVDAKVKCENNQSKKSRQVVRNLEKIIFGDYDKIDKTCDGKYAELDKSCLKRYED 387
           V D +++C   + K +++   ++ K +  D +KIDK      A+ + S LK  +D
Sbjct: 301 VTDFQIRCPMKEKKYNKECANSVIKSLGLDVEKIDKCMGDPNADTENSILKEEQD 355
>sp|P34278|YKK1_CAEEL Hypothetical protein C02F5.1 in chromosome III
          Length = 1010

 Score = 31.6 bits (70), Expect = 0.80
 Identities = 28/102 (27%), Positives = 48/102 (47%), Gaps = 4/102 (3%)
 Frame = +1

Query: 73  DKCEELNGKIDEECEDPYEALDKECD----PKYEVLDKECLPKYVEVNANFAKSVVDAKV 240
           D+ E+L+  +D   + P EA +++ D     K +++D   +     VN   A     A+V
Sbjct: 393 DEVEDLDMDMDITEQQPCEAGNQQNDGLQLQKEDLMDISVIRDSPAVNDTMAVFQSPARV 452

Query: 241 KCENNQSKKSRQVVRNLEKIIFGDYDKIDKTCDGKYAELDKS 366
           K   N S     ++ + + I+FGD   ID+T +     L KS
Sbjct: 453 KIGANNS-----IIDSQKSIVFGDEMSIDETQNDGTLTLPKS 489
>sp|O14617|AP3D1_HUMAN Adapter-related protein complex 3 delta 1 subunit (Delta-adaptin 3)
           (AP-3 complex delta subunit) (Delta-adaptin)
          Length = 1153

 Score = 31.6 bits (70), Expect = 0.80
 Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
 Frame = +1

Query: 103 DEECEDPYEALDKECDPKYEVLDKECLPKYVEVNANFAKS------VVDAKVKCENNQSK 264
           D++  DPY ALD + D    + D E LP     N   +KS      +V+ K K    + K
Sbjct: 792 DKDPNDPYRALDIDLDK--PLADSEKLPIQKHRNTETSKSPEKDVPMVEKKSKKPKKKEK 849

Query: 265 KSRQVVRNLEK 297
           K ++  R+ EK
Sbjct: 850 KHKEKERDKEK 860
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.316    0.138    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,580,561
Number of Sequences: 369166
Number of extensions: 735716
Number of successful extensions: 2262
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2250
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2294548080
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)