Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_M02
(599 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 109 5e-24
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 109 6e-24
sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 ... 106 5e-23
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 104 1e-22
sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 ... 103 3e-22
sp|Q62829|PAK3_RAT Serine/threonine-protein kinase PAK 3 (p... 102 7e-22
sp|O75914|PAK3_HUMAN Serine/threonine-protein kinase PAK 3 ... 102 7e-22
sp|Q61036|PAK3_MOUSE Serine/threonine-protein kinase PAK 3 ... 102 7e-22
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 102 1e-21
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 101 1e-21
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 109 bits (273), Expect = 5e-24
Identities = 67/165 (40%), Positives = 96/165 (58%), Gaps = 2/165 (1%)
Frame = +3
Query: 3 SIIR-FSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFR 179
S++R +++Q+L L++LH+ I+HRD+KG IL+DN IKL F ++K + ++ T
Sbjct: 172 SVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTG- 230
Query: 180 NHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRL 356
A+S GT W APEVI G+ SADIWS G ++E++TGKAP AI+ +
Sbjct: 231 ---AKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFI 287
Query: 357 GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFL 491
GT K SDA K F+ CL P RPT +L+KH F+
Sbjct: 288 GTTKSHPPIPDTLSSDA-KDFLLKCLQEVPNLRPTASELLKHPFV 331
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 109 bits (272), Expect = 6e-24
Identities = 65/165 (39%), Positives = 94/165 (56%), Gaps = 2/165 (1%)
Frame = +3
Query: 3 SIIR-FSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFR 179
S++R ++ Q+L L++LH+ I+HRD+KG IL+DN IKL F ++K + ++ +
Sbjct: 171 SVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISG- 229
Query: 180 NHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRL 356
A+S GT W APEVI G+ SADIWS G ++E++TGKAP AI+ +
Sbjct: 230 ---AKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHI 286
Query: 357 GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFL 491
GT K P S F+ CL EP RPT +L+KH F+
Sbjct: 287 GTTKS-HPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330
>sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK)
Length = 545
Score = 106 bits (264), Expect = 5e-23
Identities = 57/161 (35%), Positives = 89/161 (55%), Gaps = 1/161 (0%)
Frame = +3
Query: 21 KQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAES 197
++ L+AL+FLHS ++HRD+K ILL + +KL F A++ P+ S + +
Sbjct: 371 RECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------T 423
Query: 198 NHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIE 377
GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E
Sbjct: 424 MVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPE 483
Query: 378 FAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFLNSA 500
P K S + F++ CLD + KR + K+L++H+FL A
Sbjct: 484 LQNPEKLSAIFRDFLNRCLDMDVEKRGSAKELLQHQFLKIA 524
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 104 bits (260), Expect = 1e-22
Identities = 56/161 (34%), Positives = 89/161 (55%), Gaps = 1/161 (0%)
Frame = +3
Query: 21 KQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAES 197
++ L+AL+FLHS ++HRD+K ILL + +KL F A++ P+ S + +
Sbjct: 370 RECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------T 422
Query: 198 NHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIE 377
GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E
Sbjct: 423 MVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPE 482
Query: 378 FAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFLNSA 500
P K S + F++ CL+ + KR + K+L++H+FL A
Sbjct: 483 LQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIA 523
>sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK) (CDC42/RAC effector kinase
PAK-A)
Length = 545
Score = 103 bits (258), Expect = 3e-22
Identities = 56/161 (34%), Positives = 88/161 (54%), Gaps = 1/161 (0%)
Frame = +3
Query: 21 KQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAES 197
++ L+AL+FLHS ++HRD+K ILL + +KL F A++ P+ S + +
Sbjct: 371 RECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------T 423
Query: 198 NHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIE 377
GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E
Sbjct: 424 MVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPE 483
Query: 378 FAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFLNSA 500
P K S + F+ CL+ + KR + K+L++H+FL A
Sbjct: 484 LQNPEKLSAIFRDFLQCCLEMDVEKRGSAKELLQHQFLKIA 524
>sp|Q62829|PAK3_RAT Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3) (Beta-PAK) (p65-PAK)
Length = 544
Score = 102 bits (254), Expect = 7e-22
Identities = 54/161 (33%), Positives = 88/161 (54%), Gaps = 1/161 (0%)
Frame = +3
Query: 21 KQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAES 197
++ L+ALDFLHS ++HRD+K ILL + +KL F A++ P+ S + +
Sbjct: 369 RECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------T 421
Query: 198 NHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIE 377
GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y + T E
Sbjct: 422 MVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPE 481
Query: 378 FAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFLNSA 500
P + S + F++ CL+ + +R + K+L++H FL A
Sbjct: 482 LQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLA 522
>sp|O75914|PAK3_HUMAN Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3) (Beta-PAK) (Oligophrenin-3)
sp|Q7YQL3|PAK3_PONPY Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3)
sp|Q7YQL4|PAK3_PANTR Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3)
Length = 559
Score = 102 bits (254), Expect = 7e-22
Identities = 54/161 (33%), Positives = 88/161 (54%), Gaps = 1/161 (0%)
Frame = +3
Query: 21 KQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAES 197
++ L+ALDFLHS ++HRD+K ILL + +KL F A++ P+ S + +
Sbjct: 384 RECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------T 436
Query: 198 NHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIE 377
GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y + T E
Sbjct: 437 MVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPE 496
Query: 378 FAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFLNSA 500
P + S + F++ CL+ + +R + K+L++H FL A
Sbjct: 497 LQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLA 537
>sp|Q61036|PAK3_MOUSE Serine/threonine-protein kinase PAK 3 (p21-activated kinase 3)
(PAK-3) (Beta-PAK) (CDC42/RAC effector kinase PAK-B)
Length = 559
Score = 102 bits (254), Expect = 7e-22
Identities = 54/161 (33%), Positives = 88/161 (54%), Gaps = 1/161 (0%)
Frame = +3
Query: 21 KQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAES 197
++ L+ALDFLHS ++HRD+K ILL + +KL F A++ P+ S + +
Sbjct: 384 RECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------T 436
Query: 198 NHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIE 377
GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y + T E
Sbjct: 437 MVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPE 496
Query: 378 FAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFLNSA 500
P + S + F++ CL+ + +R + K+L++H FL A
Sbjct: 497 LQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLA 537
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 102 bits (253), Expect = 1e-21
Identities = 61/163 (37%), Positives = 91/163 (55%), Gaps = 1/163 (0%)
Frame = +3
Query: 6 IIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNH 185
II ++KQ+L L++LH+ I+HRD+KG IL+DN I+L F ++K + ++ +
Sbjct: 173 IIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATV----N 228
Query: 186 VAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGT 362
A+S GT W APEVI G+ SADIWS G ++E+ TGK P F A+ +G
Sbjct: 229 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIG- 287
Query: 363 QKKIEFAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFL 491
+ K P S K F+ CL EP R + +L++H F+
Sbjct: 288 RTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 101 bits (252), Expect = 1e-21
Identities = 60/159 (37%), Positives = 85/159 (53%), Gaps = 2/159 (1%)
Frame = +3
Query: 21 KQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESN 200
++IL+ LD+LHS+ +HRD+K +LL + ++KL F A D + RN
Sbjct: 138 REILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIK--RNTFV--- 192
Query: 201 HGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRL--GTQKKI 374
GT W APEVI Y S ADIWS G +EL G+ P L + ++ + +
Sbjct: 193 -GTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTL 251
Query: 375 EFAYPLKCSDALKSFVDPCLDPEPLKRPTVKDLMKHEFL 491
E Y S LK FV+ CL+ EP RPT K+L+KH+F+
Sbjct: 252 EGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 286
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,724,052
Number of Sequences: 369166
Number of extensions: 1404011
Number of successful extensions: 6817
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5391
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4602033670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)