Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_L21
(443 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P46507|PRS6B_MANSE 26S protease regulatory subunit 6B (A... 223 1e-58
sp|P43686|PRS6B_HUMAN 26S protease regulatory subunit 6B (M... 212 3e-55
sp|Q63570|PRS6B_RAT 26S protease regulatory subunit 6B (TAT... 212 3e-55
sp|P54775|PRS6B_MOUSE 26S protease regulatory subunit 6B (M... 205 4e-53
sp|Q9SEI4|PRS6B_ARATH 26S protease regulatory subunit 6B ho... 200 1e-51
sp|P54778|PRS6B_SOLTU 26S protease regulatory subunit 6B ho... 193 1e-49
sp|P78578|PRS6B_ASPNG 26S protease regulatory subunit 6B ho... 184 8e-47
sp|O74894|PRS6B_SCHPO 26S protease regulatory subunit 6B ho... 181 8e-46
sp|P46502|PRS6B_CAEEL Probable 26S protease regulatory subu... 180 1e-45
sp|P33298|PRS6B_YEAST 26S protease regulatory subunit 6B ho... 179 2e-45
>sp|P46507|PRS6B_MANSE 26S protease regulatory subunit 6B (ATPase MS73)
Length = 415
Score = 223 bits (568), Expect = 1e-58
Identities = 112/134 (83%), Positives = 125/134 (93%)
Frame = +1
Query: 40 QLSDEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRIKSVPLVIGQFL 219
+L E+LY K KKL++ L+F+EVQEEYIKDEQ+NLKKEYL AQEEVKRI+SVPLVIGQFL
Sbjct: 31 ELESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFL 90
Query: 220 EAVDENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDVLPPEADTSITM 399
EAVD+NTGI GSTTGSNYYVRILSTID+ELLKP+ASVALHKHSNALVDVLPPEAD+SI+M
Sbjct: 91 EAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISM 150
Query: 400 LQADEKPDVTYSMI 441
LQADEKPDV YS I
Sbjct: 151 LQADEKPDVQYSDI 164
>sp|P43686|PRS6B_HUMAN 26S protease regulatory subunit 6B (MIP224) (MB67-interacting
protein) (TAT-binding protein 7) (TBP-7)
Length = 418
Score = 212 bits (539), Expect = 3e-55
Identities = 107/145 (73%), Positives = 124/145 (85%)
Frame = +1
Query: 7 PYLNFGHIDPLQLSDEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRI 186
P + P E+LY + KKL+++L+F+EVQEEYIKDEQKNLKKE+L AQEEVKRI
Sbjct: 23 PQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRI 82
Query: 187 KSVPLVIGQFLEAVDENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDV 366
+S+PLVIGQFLEAVD+NT I GSTTGSNYYVRILSTID+ELLKPNASVALHKHSNALVDV
Sbjct: 83 QSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDV 142
Query: 367 LPPEADTSITMLQADEKPDVTYSMI 441
LPPEAD+SI ML +D+KPDV Y+ I
Sbjct: 143 LPPEADSSIMMLTSDQKPDVMYADI 167
>sp|Q63570|PRS6B_RAT 26S protease regulatory subunit 6B (TAT-binding protein 7) (TBP-7)
Length = 418
Score = 212 bits (539), Expect = 3e-55
Identities = 107/145 (73%), Positives = 124/145 (85%)
Frame = +1
Query: 7 PYLNFGHIDPLQLSDEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRI 186
P + P E+LY + KKL+++L+F+EVQEEYIKDEQKNLKKE+L AQEEVKRI
Sbjct: 23 PQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRI 82
Query: 187 KSVPLVIGQFLEAVDENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDV 366
+S+PLVIGQFLEAVD+NT I GSTTGSNYYVRILSTID+ELLKPNASVALHKHSNALVDV
Sbjct: 83 QSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDV 142
Query: 367 LPPEADTSITMLQADEKPDVTYSMI 441
LPPEAD+SI ML +D+KPDV Y+ I
Sbjct: 143 LPPEADSSIMMLTSDQKPDVMYADI 167
>sp|P54775|PRS6B_MOUSE 26S protease regulatory subunit 6B (MIP224) (MB67-interacting
protein) (TAT-binding protein 7) (TBP-7) (CIP21)
Length = 418
Score = 205 bits (521), Expect = 4e-53
Identities = 103/145 (71%), Positives = 122/145 (84%)
Frame = +1
Query: 7 PYLNFGHIDPLQLSDEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRI 186
P + P E+LY + KKL+++L+F+EVQEEYIKDEQKNLKKE+L AQEEVKRI
Sbjct: 23 PQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRI 82
Query: 187 KSVPLVIGQFLEAVDENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDV 366
+S+PLVIGQFLEAVD+NT I STTGSNYYVR++STID+ELLKPNASVALHKHSNALVDV
Sbjct: 83 QSIPLVIGQFLEAVDQNTAIVASTTGSNYYVRMVSTIDRELLKPNASVALHKHSNALVDV 142
Query: 367 LPPEADTSITMLQADEKPDVTYSMI 441
LPP AD+SI ML +D+KPDV Y+ I
Sbjct: 143 LPPAADSSIMMLTSDQKPDVMYADI 167
>sp|Q9SEI4|PRS6B_ARATH 26S protease regulatory subunit 6B homolog (26S proteasome
AAA-ATPase subunit RPT3) (Regulatory particle triple-A
ATPase subunit 3)
Length = 408
Score = 200 bits (509), Expect = 1e-51
Identities = 97/131 (74%), Positives = 119/131 (90%)
Frame = +1
Query: 49 DEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRIKSVPLVIGQFLEAV 228
+E+LY ++K LE+QL+F ++QEEY+KDEQKNLK+E L AQEEVKRI+SVPLVIGQF+E V
Sbjct: 27 EEDLYGRLKSLERQLEFTDIQEEYVKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMV 86
Query: 229 DENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDVLPPEADTSITMLQA 408
D+N GI GSTTGSNYYVRILSTI++ELLKP+ASVALH+HSNALVDVLPPEAD+SI++L
Sbjct: 87 DQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQ 146
Query: 409 DEKPDVTYSMI 441
EKPDV+Y+ I
Sbjct: 147 SEKPDVSYNDI 157
>sp|P54778|PRS6B_SOLTU 26S protease regulatory subunit 6B homolog
Length = 413
Score = 193 bits (491), Expect = 1e-49
Identities = 92/131 (70%), Positives = 117/131 (89%)
Frame = +1
Query: 49 DEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRIKSVPLVIGQFLEAV 228
+++LY ++K L++QL+FIE+QEEY+KDE KNL++E+L AQEEVKRI+SVPLVIGQF+E +
Sbjct: 32 EDDLYARLKSLQRQLEFIEIQEEYVKDELKNLRREHLRAQEEVKRIQSVPLVIGQFMEMI 91
Query: 229 DENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDVLPPEADTSITMLQA 408
D+N I GSTTGSNYYVRILSTI++ELLKP+ASV L +HSNALVDVLPPEAD+SI++L
Sbjct: 92 DQNNAIVGSTTGSNYYVRILSTINRELLKPSASVGLDRHSNALVDVLPPEADSSISLLSQ 151
Query: 409 DEKPDVTYSMI 441
EKPDVTY+ I
Sbjct: 152 SEKPDVTYNDI 162
>sp|P78578|PRS6B_ASPNG 26S protease regulatory subunit 6B homolog
Length = 423
Score = 184 bits (467), Expect = 8e-47
Identities = 89/130 (68%), Positives = 116/130 (89%)
Frame = +1
Query: 46 SDEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRIKSVPLVIGQFLEA 225
SDE Y +K+L++ L++I++QEEYIKDEQ++LK+E + AQEE+KRI+SVPLVIGQF+EA
Sbjct: 39 SDE--YATLKRLQRHLEYIQLQEEYIKDEQRSLKRELVRAQEEIKRIQSVPLVIGQFMEA 96
Query: 226 VDENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDVLPPEADTSITMLQ 405
+D+NTGI S+TGSNY VRILST+D+E LKP++SVALH+HSNALVD+LPPEAD+SI ML
Sbjct: 97 IDQNTGIVQSSTGSNYVVRILSTLDREKLKPSSSVALHRHSNALVDILPPEADSSIAMLG 156
Query: 406 ADEKPDVTYS 435
+EKPDVTY+
Sbjct: 157 ENEKPDVTYA 166
>sp|O74894|PRS6B_SCHPO 26S protease regulatory subunit 6B homolog
Length = 389
Score = 181 bits (458), Expect = 8e-46
Identities = 87/127 (68%), Positives = 114/127 (89%)
Frame = +1
Query: 55 ELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRIKSVPLVIGQFLEAVDE 234
+LY + K LE+QL+ +++QE +IK++ K+LK+E + AQEEVKRI+SVPLVIGQFLEA+D+
Sbjct: 8 DLYNRWKALERQLEMLDLQEGFIKEDCKSLKRELIRAQEEVKRIQSVPLVIGQFLEAIDQ 67
Query: 235 NTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDVLPPEADTSITMLQADE 414
NT I GSTTGSNY VRILST+D+ELLKP+ASVAL +HSNALVD+LPPEAD+SI+ML+ DE
Sbjct: 68 NTAIVGSTTGSNYVVRILSTLDRELLKPSASVALQRHSNALVDILPPEADSSISMLRPDE 127
Query: 415 KPDVTYS 435
+PDV+Y+
Sbjct: 128 RPDVSYA 134
>sp|P46502|PRS6B_CAEEL Probable 26S protease regulatory subunit 6B
Length = 414
Score = 180 bits (457), Expect = 1e-45
Identities = 90/127 (70%), Positives = 108/127 (85%)
Frame = +1
Query: 61 YVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRIKSVPLVIGQFLEAVDENT 240
Y+K+ LE+QL ++V E+YIK E +NL+KE L AQEEVKRI+SVPLVIGQFLEAVD+N
Sbjct: 37 YIKLTTLERQLAHLQVMEDYIKLETRNLEKELLHAQEEVKRIQSVPLVIGQFLEAVDQNH 96
Query: 241 GIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDVLPPEADTSITMLQADEKP 420
I GSTTGSNYYVR+LS +D+ELLKP SVALHK+SNALVDVLPPEAD+SI ML+ DEKP
Sbjct: 97 AIVGSTTGSNYYVRVLSILDRELLKPGCSVALHKYSNALVDVLPPEADSSIQMLRPDEKP 156
Query: 421 DVTYSMI 441
D++Y I
Sbjct: 157 DISYGDI 163
>sp|P33298|PRS6B_YEAST 26S protease regulatory subunit 6B homolog (YNT1 protein)
(TAT-binding homolog 2)
Length = 428
Score = 179 bits (454), Expect = 2e-45
Identities = 84/129 (65%), Positives = 113/129 (87%)
Frame = +1
Query: 49 DEELYVKMKKLEKQLQFIEVQEEYIKDEQKNLKKEYLLAQEEVKRIKSVPLVIGQFLEAV 228
+ ++Y K+KKLEK+ + + +QE+YIKDEQ++LK+E AQEEVKRI+SVPLVIGQFLE +
Sbjct: 44 NSDIYFKLKKLEKEYELLTLQEDYIKDEQRHLKRELKRAQEEVKRIQSVPLVIGQFLEPI 103
Query: 229 DENTGIDGSTTGSNYYVRILSTIDKELLKPNASVALHKHSNALVDVLPPEADTSITMLQA 408
D+NTGI STTG +Y VRILST+D+ELLKP+ SVALH+HSNALVD+LPP++D+SI+++
Sbjct: 104 DQNTGIVSSTTGMSYVVRILSTLDRELLKPSMSVALHRHSNALVDILPPDSDSSISVMGE 163
Query: 409 DEKPDVTYS 435
+EKPDVTY+
Sbjct: 164 NEKPDVTYA 172
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.312 0.132 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,775,160
Number of Sequences: 369166
Number of extensions: 663168
Number of successful extensions: 2459
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2290
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2447
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2344429560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)