Planarian EST Database


Dr_sW_025_L14

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_025_L14
         (601 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8Y9G3|HIS5_LISMO  Imidazole glycerol phosphate synthase ...    31   2.7  
sp|Q5I043|UBP28_MOUSE  Ubiquitin carboxyl-terminal hydrolase...    30   4.7  
sp|Q96RU2|UBP28_HUMAN  Ubiquitin carboxyl-terminal hydrolase...    30   4.7  
sp|P03243|E1BL_ADE05  E1B protein, large T-antigen                 30   6.1  
sp|P03244|E1BL_ADE02  E1B protein, large T-antigen                 30   6.1  
sp|P47486|UVRD_MYCGE  Probable DNA helicase II homolog             30   6.1  
sp|Q5R4M0|GRIA2_PONPY  Glutamate receptor 2 precursor (GluR-...    30   6.1  
sp|P23819|GRIA2_MOUSE  Glutamate receptor 2 precursor (GluR-...    30   6.1  
sp|P42262|GRIA2_HUMAN  Glutamate receptor 2 precursor (GluR-...    30   6.1  
sp|P25355|YCU4_YEAST  Hypothetical 65.2 kDa protein in THR4-...    29   7.9  
>sp|Q8Y9G3|HIS5_LISMO Imidazole glycerol phosphate synthase subunit hisH (IGP synthase
           glutamine amidotransferase subunit) (IGP synthase
           subunit hisH) (ImGP synthase subunit hisH) (IGPS subunit
           hisH)
          Length = 208

 Score = 30.8 bits (68), Expect = 2.7
 Identities = 26/86 (30%), Positives = 33/86 (38%), Gaps = 10/86 (11%)
 Frame = +3

Query: 78  QSSTAQPASNANDGNYDTYSHTYNYGPPTLDIDLQREYPISVVTLVNIKHCCHGNIVGAV 257
           Q   A P +   DG Y  Y H+Y    P   I     Y I V  ++N      GNI GA 
Sbjct: 125 QIKRATPLTKQLDGEYVYYVHSYYANCPEEYIIATSGYSIEVPGMIN-----KGNIYGAQ 179

Query: 258 ----------IELLSRSDKVLYQCGS 305
                     +E+L    +V Y C S
Sbjct: 180 FHPEKSGQIGLEILKGFKEVTYSCKS 205
>sp|Q5I043|UBP28_MOUSE Ubiquitin carboxyl-terminal hydrolase 28 (Ubiquitin thiolesterase
           28) (Ubiquitin-specific processing protease 28)
           (Deubiquitinating enzyme 28)
          Length = 1051

 Score = 30.0 bits (66), Expect = 4.7
 Identities = 33/136 (24%), Positives = 55/136 (40%), Gaps = 10/136 (7%)
 Frame = +3

Query: 153 GPPTLDIDLQREYPISVVTLVNIKHCCHGNIVGAVIELLSRSDKVLY-QCGSITDIRLVY 329
           G P  + +   +YP+ V    N+  C  G +V   I LL     V Y Q    T +  V 
Sbjct: 305 GKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQERWFTKLPPVL 364

Query: 330 TFSCR----NEYASKIRVRHTRNDYIHIAELEVYTESDSDFLHS-----RKGKNDIHVAM 482
           TF       N+   +    H + ++  I  ++ Y     + + S     RK K +I V +
Sbjct: 365 TFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYKSKELIRSKRESVRKLKEEIQV-L 423

Query: 483 KSKFFQVLIKGNNPRR 530
           + K  + +  G+ P R
Sbjct: 424 QQKLERYVKYGSGPSR 439
>sp|Q96RU2|UBP28_HUMAN Ubiquitin carboxyl-terminal hydrolase 28 (Ubiquitin thiolesterase
           28) (Ubiquitin-specific processing protease 28)
           (Deubiquitinating enzyme 28)
          Length = 1077

 Score = 30.0 bits (66), Expect = 4.7
 Identities = 31/136 (22%), Positives = 56/136 (41%), Gaps = 10/136 (7%)
 Frame = +3

Query: 153 GPPTLDIDLQREYPISVVTLVNIKHCCHGNIVGAVIELLSRSDKVLY-QCGSITDIRLVY 329
           G P  + +   +YP+ V    N+  C  G +V   +ELL     V Y Q    T +  V 
Sbjct: 304 GKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVL 363

Query: 330 TFSCR----NEYASKIRVRHTRNDYIHIAELEVYTESDSDFLHS-----RKGKNDIHVAM 482
           TF       N+   +    H + ++  I  ++ Y     + + +     RK K +I + +
Sbjct: 364 TFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKI-L 422

Query: 483 KSKFFQVLIKGNNPRR 530
           + K  + +  G+ P R
Sbjct: 423 QQKLERYVKYGSGPAR 438
>sp|P03243|E1BL_ADE05 E1B protein, large T-antigen
          Length = 496

 Score = 29.6 bits (65), Expect = 6.1
 Identities = 14/46 (30%), Positives = 27/46 (58%)
 Frame = +3

Query: 171 IDLQREYPISVVTLVNIKHCCHGNIVGAVIELLSRSDKVLYQCGSI 308
           + L+ +    +  LVNI++CC+ +  GA +E +   D+V ++C  I
Sbjct: 178 VALRPDCKYKISKLVNIRNCCYISGNGAEVE-IDTEDRVAFRCSMI 222
>sp|P03244|E1BL_ADE02 E1B protein, large T-antigen
          Length = 495

 Score = 29.6 bits (65), Expect = 6.1
 Identities = 14/46 (30%), Positives = 27/46 (58%)
 Frame = +3

Query: 171 IDLQREYPISVVTLVNIKHCCHGNIVGAVIELLSRSDKVLYQCGSI 308
           + L+ +    +  LVNI++CC+ +  GA +E +   D+V ++C  I
Sbjct: 177 VALRPDCKYKISKLVNIRNCCYISGNGAEVE-IDTEDRVAFRCSMI 221
>sp|P47486|UVRD_MYCGE Probable DNA helicase II homolog
          Length = 703

 Score = 29.6 bits (65), Expect = 6.1
 Identities = 20/74 (27%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
 Frame = -2

Query: 582 NVFICFNKSK----KQCFFNVIDEDCCLLSTLGKISTS*QHGYRFCLFLNGENQNQIRYK 415
           N FI + K+K    K  F +++ + C LLS    +       ++  L    ++ NQI+Y+
Sbjct: 171 NAFISYEKNKILNNKLDFHDLLIKTCNLLSNDNDLLNQWSEQFQHILVDEFQDTNQIQYE 230

Query: 414 LLIQLYVYNHFLYV 373
           L+  L   N  L++
Sbjct: 231 LIKMLVTKNKNLFL 244
>sp|Q5R4M0|GRIA2_PONPY Glutamate receptor 2 precursor (GluR-2) (GluR-B) (GluR-K2)
           (Glutamate receptor ionotropic, AMPA 2) (AMPA-selective
           glutamate receptor 2)
          Length = 883

 Score = 29.6 bits (65), Expect = 6.1
 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
 Frame = +3

Query: 3   IILISFCVTNSLQNLVLISRGKPANQSSTAQPASNANDGNYDTYSHTYN-YGPPTLDI 173
           + LI FC  +  +      R K A  +    P+S+ N  N+ TY   YN YG  ++ I
Sbjct: 830 VALIEFCYKSRAE----AKRMKVAKNAQNINPSSSQNSQNFATYKEGYNVYGIESVKI 883
>sp|P23819|GRIA2_MOUSE Glutamate receptor 2 precursor (GluR-2) (GluR-B) (GluR-K2)
           (Glutamate receptor ionotropic, AMPA 2) (AMPA-selective
           glutamate receptor 2)
          Length = 883

 Score = 29.6 bits (65), Expect = 6.1
 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
 Frame = +3

Query: 3   IILISFCVTNSLQNLVLISRGKPANQSSTAQPASNANDGNYDTYSHTYN-YGPPTLDI 173
           + LI FC  +  +      R K A  +    P+S+ N  N+ TY   YN YG  ++ I
Sbjct: 830 VALIEFCYKSRAE----AKRMKVAKNAQNINPSSSQNSQNFATYKEGYNVYGIESVKI 883
>sp|P42262|GRIA2_HUMAN Glutamate receptor 2 precursor (GluR-2) (GluR-B) (GluR-K2)
           (Glutamate receptor ionotropic, AMPA 2) (AMPA-selective
           glutamate receptor 2)
          Length = 883

 Score = 29.6 bits (65), Expect = 6.1
 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
 Frame = +3

Query: 3   IILISFCVTNSLQNLVLISRGKPANQSSTAQPASNANDGNYDTYSHTYN-YGPPTLDI 173
           + LI FC  +  +      R K A  +    P+S+ N  N+ TY   YN YG  ++ I
Sbjct: 830 VALIEFCYKSRAE----AKRMKVAKNAQNINPSSSQNSQNFATYKEGYNVYGIESVKI 883
>sp|P25355|YCU4_YEAST Hypothetical 65.2 kDa protein in THR4-PWP2 intergenic region
          Length = 563

 Score = 29.3 bits (64), Expect = 7.9
 Identities = 22/70 (31%), Positives = 34/70 (48%)
 Frame = -2

Query: 450 LNGENQNQIRYKLLIQLYVYNHFLYVSHEFLMRIHFYKKMYILILYQLCFHIDTELYLIS 271
           L  ENQ +I  KLL+Q  V   FL+ + E+++     K +YI  L  L F+I      + 
Sbjct: 99  LAAENQ-EIPQKLLLQNLVIRGFLHATSEYVVDTPLIKHLYIACLTCL-FNIQQNYSTVD 156

Query: 270 IVTLSLPRQY 241
           + T     Q+
Sbjct: 157 MTTFPALLQF 166
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,101,335
Number of Sequences: 369166
Number of extensions: 1374124
Number of successful extensions: 3470
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3342
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3468
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4602033670
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)