Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_K22
(860 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q24799|MYPH_ECHGR Myophilin 164 2e-40
sp|P14318|MP20_DROME Muscle-specific protein 20 150 6e-36
sp|P37397|CNN3_RAT Calponin-3 (Calponin, acidic isoform) (C... 138 2e-32
sp|Q15417|CNN3_HUMAN Calponin-3 (Calponin, acidic isoform) 137 3e-32
sp|Q9DAW9|CNN3_MOUSE Calponin-3 (Calponin, acidic isoform) 137 4e-32
sp|Q08092|CNN1_PIG Calponin-1 (Calponin H1, smooth muscle) ... 137 5e-32
sp|Q08290|CNN1_RAT Calponin-1 (Calponin H1, smooth muscle) ... 136 9e-32
sp|Q08091|CNN1_MOUSE Calponin-1 (Calponin H1, smooth muscle... 136 9e-32
sp|P51911|CNN1_HUMAN Calponin-1 (Calponin H1, smooth muscle... 134 3e-31
sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-a... 134 4e-31
>sp|Q24799|MYPH_ECHGR Myophilin
Length = 190
Score = 164 bits (416), Expect = 2e-40
Identities = 92/202 (45%), Positives = 120/202 (59%), Gaps = 1/202 (0%)
Frame = +3
Query: 57 MSNRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILV 236
MSN G+ Q+ +KL K D + E E ++W+ +TG + + + LK+G +L
Sbjct: 1 MSNVPPPSGLSYQVKKKLEGKRDKDQENEALEWIEALTGLKLDRS-KLYEDILKDGTVLC 59
Query: 237 KLANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTV 416
KL N + G KK T PFK MENI FL+A + YGVP A LFQTV
Sbjct: 60 KLMNSIKPGCIKKIN-----------ENATMPFKIMENISAFLEAMKGYGVPVADLFQTV 108
Query: 417 DLFESRNMAQVLNTLLQLGTECQRN-EFKGPVCGPKPTYENKREFSEEQLRASQGIIGLQ 593
DLFE +++AQV TL LG CQ + E+ GPV GPK ENKREF+E+QLR Q ++ LQ
Sbjct: 109 DLFEKKDIAQVTRTLFALGRTCQTHPEYSGPVLGPKLATENKREFTEQQLREGQNVVSLQ 168
Query: 594 AGSNKGASQSGMSMGGVRHIAD 659
GSNKGASQ+G++MG R I D
Sbjct: 169 YGSNKGASQAGINMGKQRMIMD 190
>sp|P14318|MP20_DROME Muscle-specific protein 20
Length = 184
Score = 150 bits (378), Expect = 6e-36
Identities = 85/193 (44%), Positives = 117/193 (60%), Gaps = 1/193 (0%)
Frame = +3
Query: 78 MGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKLANKVY 257
M ++ + K+ SK + E +KE +W+ I E P G Q + LK+GQ+L KL N +
Sbjct: 1 MSLERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAG-QSYEDVLKDGQVLCKLINVL- 58
Query: 258 EGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDLFESRN 437
P A K+ N+ FK MENI F KA + YGVP +FQTVDL+E ++
Sbjct: 59 -----SPNAVPKV------NSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKD 107
Query: 438 MAQVLNTLLQLGTECQRN-EFKGPVCGPKPTYENKREFSEEQLRASQGIIGLQAGSNKGA 614
+A V NT+ LG ++ +FKGP GPKP E KR+F+EEQL+A Q I+GLQAGSNKGA
Sbjct: 108 IANVTNTIFALGRATYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIVGLQAGSNKGA 167
Query: 615 SQSGMSMGGVRHI 653
+Q+G ++G R I
Sbjct: 168 TQAGQNLGAGRKI 180
>sp|P37397|CNN3_RAT Calponin-3 (Calponin, acidic isoform) (Calponin, non-muscle
isoform)
Length = 330
Score = 138 bits (347), Expect = 2e-32
Identities = 89/250 (35%), Positives = 136/250 (54%), Gaps = 3/250 (1%)
Frame = +3
Query: 63 NRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKL 242
N+ G+ ++ K+ SKYD ++E+++ +W+ ++TG + Q LK+G IL +L
Sbjct: 5 NKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTGMGIGTNFQL---GLKDGIILCEL 61
Query: 243 ANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDL 422
NK+ G+ KK N + + Q+ENI F+KA +AYG+ +F+ DL
Sbjct: 62 INKLQPGSVKK------------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109
Query: 423 FESRNMAQVLNTLLQLGTECQRNEFKGPV-CGPKPTYENKREFSEEQLRASQGIIGLQAG 599
FE+ NM QV TL+ L + F + G K + R F E +L+A Q +IGLQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169
Query: 600 SNKGASQSGMSMGGV-RHIADIKVDEMSDEAKGIVGLQAGSNKGASQSGMRI-GTQRFIN 773
+NK ASQ+GM+ G RH+ D K+ + + LQ G+NKGASQ+GM GT+R I
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMSAPGTRRDIY 229
Query: 774 DVSTGVEMTD 803
D ++ D
Sbjct: 230 DQKLTLQPVD 239
>sp|Q15417|CNN3_HUMAN Calponin-3 (Calponin, acidic isoform)
Length = 329
Score = 137 bits (346), Expect = 3e-32
Identities = 89/250 (35%), Positives = 136/250 (54%), Gaps = 3/250 (1%)
Frame = +3
Query: 63 NRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKL 242
N+ G+ ++ K+ SKYD ++E+++ +W+ ++TG + Q LK+G IL +L
Sbjct: 5 NKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIG---PNFQLGLKDGIILCEL 61
Query: 243 ANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDL 422
NK+ G+ KK N + + Q+ENI F+KA +AYG+ +F+ DL
Sbjct: 62 INKLQPGSVKK------------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109
Query: 423 FESRNMAQVLNTLLQLGTECQRNEFKGPV-CGPKPTYENKREFSEEQLRASQGIIGLQAG 599
FE+ NM QV TL+ L + F + G K + R F E +L+A Q +IGLQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169
Query: 600 SNKGASQSGMSMGGV-RHIADIKVDEMSDEAKGIVGLQAGSNKGASQSGMRI-GTQRFIN 773
+NK ASQ+GM+ G RH+ D K+ + + LQ G+NKGASQ+GM GT+R I
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIY 229
Query: 774 DVSTGVEMTD 803
D ++ D
Sbjct: 230 DQKLTLQPVD 239
>sp|Q9DAW9|CNN3_MOUSE Calponin-3 (Calponin, acidic isoform)
Length = 330
Score = 137 bits (345), Expect = 4e-32
Identities = 89/250 (35%), Positives = 136/250 (54%), Gaps = 3/250 (1%)
Frame = +3
Query: 63 NRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKL 242
N+ G+ ++ K+ SKYD ++E+++ +W+ ++TG + Q LK+G IL +L
Sbjct: 5 NKGPSYGLSAEVKNKIASKYDQQAEEDLRNWIEEVTGLGIGTNFQL---GLKDGIILCEL 61
Query: 243 ANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDL 422
NK+ G+ KK N + + Q+ENI F+KA +AYG+ +F+ DL
Sbjct: 62 INKLQPGSVKK------------VNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109
Query: 423 FESRNMAQVLNTLLQLGTECQRNEFKGPV-CGPKPTYENKREFSEEQLRASQGIIGLQAG 599
FE+ NM QV TL+ L + F + G K + R F E +L+A Q +IGLQ G
Sbjct: 110 FENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMG 169
Query: 600 SNKGASQSGMSMGGV-RHIADIKVDEMSDEAKGIVGLQAGSNKGASQSGMRI-GTQRFIN 773
+NK ASQ+GM+ G RH+ D K+ + + LQ G+NKGASQ+GM GT+R I
Sbjct: 170 TNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIY 229
Query: 774 DVSTGVEMTD 803
D ++ D
Sbjct: 230 DQKLTLQPVD 239
>sp|Q08092|CNN1_PIG Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 137 bits (344), Expect = 5e-32
Identities = 92/249 (36%), Positives = 131/249 (52%), Gaps = 2/249 (0%)
Frame = +3
Query: 63 NRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKL 242
NR G+ ++ KL KYD + E+E+ +W+ +TG + LK+G IL +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQQEQELREWIEGVTGRRIG---NNFMDGLKDGIILCEF 63
Query: 243 ANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDL 422
NK+ G+ KK N T + Q+ENI F+KA YGV +F+ DL
Sbjct: 64 INKLQPGSVKK------------VNESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDL 111
Query: 423 FESRNMAQVLNTLLQLGTECQRNEFKGPVCGPKPTYENKREFSEEQLRASQGIIGLQAGS 602
FE+ N QV +TLL L + + K V G K + +R+F E+LR + IIGLQ G+
Sbjct: 112 FENTNHTQVQSTLLALASMAKTKGNKVNV-GVKYAEKQERKFEPEKLREGRNIIGLQMGT 170
Query: 603 NKGASQSGMSMGGV-RHIADIKVDEMSDEAKGIVGLQAGSNKGASQSGMRI-GTQRFIND 776
NK ASQ GM+ G RH+ D K+ + + LQ G+NKGASQ+GM GT+R I +
Sbjct: 171 NKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFE 230
Query: 777 VSTGVEMTD 803
G+E D
Sbjct: 231 PGLGMEHCD 239
Score = 31.2 bits (69), Expect = 3.9
Identities = 16/29 (55%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = +3
Query: 582 IGLQAGSNKGASQSGMSMGGV-RHIADIK 665
+ LQ GSNKGASQ GM++ G+ R + D K
Sbjct: 243 VSLQMGSNKGASQRGMTVYGLPRQVYDPK 271
>sp|Q08290|CNN1_RAT Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 136 bits (342), Expect = 9e-32
Identities = 92/249 (36%), Positives = 130/249 (52%), Gaps = 2/249 (0%)
Frame = +3
Query: 63 NRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKL 242
NR G+ ++ KL KYD + E+E+ +W+ +TG + LK+G IL +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIG---SNFMDGLKDGIILCEF 63
Query: 243 ANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDL 422
NK+ G+ KK N T + Q+ENI F+KA YGV +F+ DL
Sbjct: 64 INKLQPGSVKK------------VNESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDL 111
Query: 423 FESRNMAQVLNTLLQLGTECQRNEFKGPVCGPKPTYENKREFSEEQLRASQGIIGLQAGS 602
FE+ N QV +TLL L + + K V G K + +R F E+LR + IIGLQ G+
Sbjct: 112 FENTNHTQVQSTLLALASMAKTKGNKVNV-GVKYAEKQERRFEPEKLREGRNIIGLQMGT 170
Query: 603 NKGASQSGMSMGGV-RHIADIKVDEMSDEAKGIVGLQAGSNKGASQSGMRI-GTQRFIND 776
NK ASQ GM+ G RH+ D K+ + + LQ G+NKGASQ+GM GT+R I +
Sbjct: 171 NKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFE 230
Query: 777 VSTGVEMTD 803
G+E D
Sbjct: 231 PGLGMEHCD 239
Score = 31.2 bits (69), Expect = 3.9
Identities = 16/29 (55%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = +3
Query: 582 IGLQAGSNKGASQSGMSMGGV-RHIADIK 665
+ LQ GSNKGASQ GM++ G+ R + D K
Sbjct: 243 VSLQMGSNKGASQRGMTVYGLPRQVYDPK 271
>sp|Q08091|CNN1_MOUSE Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 136 bits (342), Expect = 9e-32
Identities = 92/249 (36%), Positives = 130/249 (52%), Gaps = 2/249 (0%)
Frame = +3
Query: 63 NRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKL 242
NR G+ ++ KL KYD + E+E+ +W+ +TG + LK+G IL +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIG---NNFMDGLKDGIILCEF 63
Query: 243 ANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDL 422
NK+ G+ KK N T + Q+ENI F+KA YGV +F+ DL
Sbjct: 64 INKLQPGSVKK------------VNESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDL 111
Query: 423 FESRNMAQVLNTLLQLGTECQRNEFKGPVCGPKPTYENKREFSEEQLRASQGIIGLQAGS 602
FE+ N QV +TLL L + + K V G K + +R F E+LR + IIGLQ G+
Sbjct: 112 FENTNHTQVQSTLLALASMAKTKGNKVNV-GVKYAEKQERRFEPEKLREGRNIIGLQMGT 170
Query: 603 NKGASQSGMSMGGV-RHIADIKVDEMSDEAKGIVGLQAGSNKGASQSGMRI-GTQRFIND 776
NK ASQ GM+ G RH+ D K+ + + LQ G+NKGASQ+GM GT+R I +
Sbjct: 171 NKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFE 230
Query: 777 VSTGVEMTD 803
G+E D
Sbjct: 231 PGLGMEHCD 239
Score = 31.2 bits (69), Expect = 3.9
Identities = 16/29 (55%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = +3
Query: 582 IGLQAGSNKGASQSGMSMGGV-RHIADIK 665
+ LQ GSNKGASQ GM++ G+ R + D K
Sbjct: 243 VSLQMGSNKGASQRGMTVYGLPRQVYDPK 271
>sp|P51911|CNN1_HUMAN Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 134 bits (337), Expect = 3e-31
Identities = 91/249 (36%), Positives = 130/249 (52%), Gaps = 2/249 (0%)
Frame = +3
Query: 63 NRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHVPYGIQQVQQALKNGQILVKL 242
NR G+ ++ KL KYD + E+E+ +W+ +TG + LK+G IL +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIG---NNFMDGLKDGIILCEF 63
Query: 243 ANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLFQTVDL 422
NK+ G+ KK N T + Q+ENI F+KA YGV +F+ DL
Sbjct: 64 INKLQPGSVKK------------INESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDL 111
Query: 423 FESRNMAQVLNTLLQLGTECQRNEFKGPVCGPKPTYENKREFSEEQLRASQGIIGLQAGS 602
FE+ N QV +TLL L + + K V G K + +R+F +LR + IIGLQ G+
Sbjct: 112 FENTNHTQVQSTLLALASMAKTKGNKVNV-GVKYAEKQERKFEPGKLREGRNIIGLQMGT 170
Query: 603 NKGASQSGMSMGGV-RHIADIKVDEMSDEAKGIVGLQAGSNKGASQSGMRI-GTQRFIND 776
NK ASQ GM+ G RH+ D K+ + + LQ G+NKGASQ+GM GT+R I +
Sbjct: 171 NKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFE 230
Query: 777 VSTGVEMTD 803
G+E D
Sbjct: 231 PGLGMEHCD 239
Score = 31.2 bits (69), Expect = 3.9
Identities = 16/29 (55%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = +3
Query: 582 IGLQAGSNKGASQSGMSMGGV-RHIADIK 665
+ LQ GSNKGASQ GM++ G+ R + D K
Sbjct: 243 VSLQMGSNKGASQRGMTVYGLPRQVYDPK 271
>sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-alpha) (SM22-alpha)
Length = 200
Score = 134 bits (336), Expect = 4e-31
Identities = 88/206 (42%), Positives = 114/206 (55%), Gaps = 7/206 (3%)
Frame = +3
Query: 57 MSNRIGDMGMDVQINRKLNSKYDSESEKEVIDWLNQITGEHV---PYGIQQVQQALKNGQ 227
M+N+ GM + K+ KYD E E +++W+ G V G Q LKNG
Sbjct: 1 MANKGPAYGMSRDVQSKIEKKYDDELEDRLVEWIVAQCGSSVGRPDRGRLGFQVWLKNGI 60
Query: 228 ILVKLANKVYEGTDKKPQAALKIKGPIKFNTMTAPFKQMENIQLFLKAAEAYGVPRASLF 407
+L +L N +Y P + +K P TM FKQME I FLKAAE YGV + +F
Sbjct: 61 VLSQLVNSLY------PDGSKPVKIPDSPPTMV--FKQMEQIAQFLKAAEDYGVVKTDMF 112
Query: 408 QTVDLFESRNMAQVLNTLLQLG----TECQRNEFKGPVCGPKPTYENKREFSEEQLRASQ 575
QTVDLFE+++MA V TL+ LG T+ + P K E+KREFSE QL+ +
Sbjct: 113 QTVDLFEAKDMAAVQRTLVALGSLAVTKNDGHYHGDPNWFMKKAQEHKREFSESQLKEGK 172
Query: 576 GIIGLQAGSNKGASQSGMSMGGVRHI 653
IIGLQ G+NKGASQ+GMS G R I
Sbjct: 173 NIIGLQMGTNKGASQAGMSYGRPRQI 198
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,034,236
Number of Sequences: 369166
Number of extensions: 1859232
Number of successful extensions: 5176
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4820
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5056
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8534739105
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)