Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_K21
(275 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P31713|ISH1_STOHE Kunitz-type proteinase inhibitor SHPI-1 69 4e-12
sp|P81129|ISH2_STOHE Kunitz-type proteinase inhibitor SHPI-2 68 6e-12
sp|P81902|CSTI_BOMMO Trypsin inhibitor (Cocoon shell-associ... 68 8e-12
sp|P81906|ISPI2_GALME Inducible serine protease inhibitor 2... 65 5e-11
sp|Q9BDL1|EPPI_MACMU Eppin precursor (Epididymal protease i... 63 2e-10
sp|O95925|EPPI_HUMAN Eppin precursor (Epididymal protease i... 62 3e-10
sp|Q9TWF8|KC3_ANESU Kunitz-type kalicludine 3 (AsKC3) 61 9e-10
sp|P82968|MCPI_MELCP Protease inhibitor (McaPI) 61 9e-10
sp|P00994|ISIK_HELPO Isoinhibitor K 60 1e-09
sp|P12023|A4_MOUSE Amyloid beta A4 protein precursor (APP) ... 60 2e-09
>sp|P31713|ISH1_STOHE Kunitz-type proteinase inhibitor SHPI-1
Length = 55
Score = 68.6 bits (166), Expect = 4e-12
Identities = 27/51 (52%), Positives = 35/51 (68%)
Frame = +3
Query: 54 CNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARC 206
C++P + G C+ YF R+ FD +TGKCT F+YGGC N NNF T +C A C
Sbjct: 3 CSEPKKVGRCKGYFPRFYFDSETGKCTPFIYGGCGGNGNNFETLHQCRAIC 53
>sp|P81129|ISH2_STOHE Kunitz-type proteinase inhibitor SHPI-2
Length = 55
Score = 68.2 bits (165), Expect = 6e-12
Identities = 28/51 (54%), Positives = 33/51 (64%)
Frame = +3
Query: 54 CNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARC 206
C +P G C+ YF R+ FD KTGKCT F+YGGC N NNF T +C A C
Sbjct: 3 CLEPKRVGRCKGYFPRFYFDSKTGKCTPFIYGGCGGNGNNFETLHQCRAIC 53
>sp|P81902|CSTI_BOMMO Trypsin inhibitor (Cocoon shell-associated trypsin inhibitor)
(CSTI)
Length = 55
Score = 67.8 bits (164), Expect = 8e-12
Identities = 27/53 (50%), Positives = 33/53 (62%)
Frame = +3
Query: 48 PECNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARC 206
P+C P++TG C+ F RY +D KC F+YGGC N NNF T ECEA C
Sbjct: 2 PDCLLPIKTGPCKGSFPRYAYDSSEDKCVEFIYGGCQANANNFETIEECEAAC 54
>sp|P81906|ISPI2_GALME Inducible serine protease inhibitor 2 (ISPI-2)
Length = 52
Score = 65.1 bits (157), Expect = 5e-11
Identities = 27/49 (55%), Positives = 33/49 (67%)
Frame = +3
Query: 48 PECNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAEC 194
P+C PLETG+CRA R+G+D K +CT FVYGGC N+NNF C
Sbjct: 3 PKCTLPLETGICRAELHRFGYDTKLKECTQFVYGGCHHNENNFKKLEVC 51
>sp|Q9BDL1|EPPI_MACMU Eppin precursor (Epididymal protease inhibitor) (Serine protease
inhibitor-like with Kunitz and WAP domains 1)
Length = 133
Score = 63.2 bits (152), Expect = 2e-10
Identities = 25/52 (48%), Positives = 34/52 (65%)
Frame = +3
Query: 54 CNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARCE 209
C P ETG C A+F+R+ +D K C+TFV+GGC N+NNF + A C C+
Sbjct: 77 CEMPNETGPCLAFFIRWWYDKKNNTCSTFVHGGCQGNNNNFQSEANCLNTCK 128
>sp|O95925|EPPI_HUMAN Eppin precursor (Epididymal protease inhibitor) (Serine protease
inhibitor-like with Kunitz and WAP domains 1) (WAP
four-disulfide core domain protein 7) (Protease
inhibitor WAP7)
Length = 133
Score = 62.4 bits (150), Expect = 3e-10
Identities = 25/52 (48%), Positives = 33/52 (63%)
Frame = +3
Query: 54 CNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARCE 209
C P ETG C AYF+ + +D K C+ FVYGGC N+NNF ++A C C+
Sbjct: 77 CEMPKETGPCLAYFLHWWYDKKDNTCSMFVYGGCQGNNNNFQSKANCLNTCK 128
>sp|Q9TWF8|KC3_ANESU Kunitz-type kalicludine 3 (AsKC3)
Length = 59
Score = 60.8 bits (146), Expect = 9e-10
Identities = 25/52 (48%), Positives = 32/52 (61%)
Frame = +3
Query: 51 ECNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARC 206
+C P G CRA F RY +++ + +C F+YGGC N NNFHT ECE C
Sbjct: 4 DCELPKVVGRCRARFPRYYYNLSSRRCEKFIYGGCGGNANNFHTLEECEKVC 55
>sp|P82968|MCPI_MELCP Protease inhibitor (McaPI)
Length = 197
Score = 60.8 bits (146), Expect = 9e-10
Identities = 31/60 (51%), Positives = 34/60 (56%)
Frame = +3
Query: 27 HFGESRAPECNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARC 206
H GE A C+ G CR F RY FD +GKC FVYGGC NDNNF T C+ RC
Sbjct: 137 HPGECDA--CSLKKVVGPCRGAFRRYYFDSVSGKCEEFVYGGCGGNDNNFKTLDACQKRC 194
>sp|P00994|ISIK_HELPO Isoinhibitor K
Length = 58
Score = 60.5 bits (145), Expect = 1e-09
Identities = 25/57 (43%), Positives = 34/57 (59%)
Frame = +3
Query: 36 ESRAPECNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARC 206
+ R CN P ETG C+A F +Y ++ K+G C F+YGGC N N F T +C+ C
Sbjct: 1 QGRPSFCNLPAETGPCKASFRQYYYNSKSGGCQQFIYGGCRGNQNRFDTTQQCQGVC 57
>sp|P12023|A4_MOUSE Amyloid beta A4 protein precursor (APP) (ABPP) (Alzheimer's disease
amyloid protein homolog) (Amyloidogenic glycoprotein)
(AG) [Contains: Soluble APP-alpha (S-APP-alpha); Soluble
APP-beta (S-APP-beta); C99 (APP-C99); Beta-amyloid
protein 42 (Beta-APP42); Beta-amyloid protein 40
(Beta-APP40); C83; P3(42); P3(40); Gamma-CTF(59)
(Gamma-secretase C-terminal fragment 59) (Amyloid
intracellular domain 59) (AID(59)) (APP-C59);
Gamma-CTF(57) (Gamma-secretase C-terminal fragment 57)
(Amyloid intracellular domain 57) (AID(57)) (APP-C57);
Gamma-CTF(50) (Gamma-secretase C-terminal fragment 50)
(Amyloid intracellular domain 50) (AID(50)); C31]
Length = 770
Score = 59.7 bits (143), Expect = 2e-09
Identities = 27/51 (52%), Positives = 30/51 (58%)
Frame = +3
Query: 54 CNQPLETGLCRAYFVRYGFDVKTGKCTTFVYGGCPVNDNNFHTRAECEARC 206
C++ ETG CRA R+ FDV GKC F YGGC N NNF T C A C
Sbjct: 291 CSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVC 341
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,119,725
Number of Sequences: 369166
Number of extensions: 522907
Number of successful extensions: 1456
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1408
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1453
length of database: 68,354,980
effective HSP length: 61
effective length of database: 57,086,145
effective search space used: 1712584350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)