Planarian EST Database


Dr_sW_025_K12

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_025_K12
         (873 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q02455|MLP1_YEAST  Protein MLP1 (Myosin-like protein 1)         33   0.79 
sp|Q04347|BUD22_YEAST  Bud site selection protein 22               33   0.79 
sp|P31916|MAT2_EUGGR  Maturase-like protein 2                      32   1.8  
sp|Q9UZC8|RAD50_PYRAB  DNA double-strand break repair rad50 ...    32   1.8  
sp|P10499|KCNA1_RAT  Potassium voltage-gated channel subfami...    32   2.3  
sp|P16388|KCNA1_MOUSE  Potassium voltage-gated channel subfa...    32   2.3  
sp|Q09470|KCNA1_HUMAN  Potassium voltage-gated channel subfa...    32   2.3  
sp|P25386|USO1_YEAST  Intracellular protein transport protei...    32   2.3  
sp|P49947|FRIM_SALSA  Ferritin, middle subunit (Ferritin M)        32   2.3  
sp|O66834|RECN_AQUAE  DNA repair protein recN (Recombination...    31   5.1  
>sp|Q02455|MLP1_YEAST Protein MLP1 (Myosin-like protein 1)
          Length = 1875

 Score = 33.5 bits (75), Expect = 0.79
 Identities = 47/216 (21%), Positives = 94/216 (43%), Gaps = 11/216 (5%)
 Frame = +2

Query: 80   DATENCAIINKKISERFYYSNSLNQPVEKSNDXXXXXXXXXXXXXYSRTFSDKVMSHSNT 259
            ++TEN +++NK+I + +   + ++  + K                     S ++++    
Sbjct: 653  ESTENMSLLNKEIQDLYDSKSDISIKLGKEK-------------------SSRILAEERF 693

Query: 260  SL--NKLDLFSSKNENWQIVQEALTNALL---GKNVKNLNEVLGC---IRQVDEQFANNN 415
             L  N LDL  ++N+  +   + L N +L    K  + LNE + C   +  V+ +  N  
Sbjct: 694  KLLSNTLDLTKAENDQLRKRFDYLQNTILKQDSKTHETLNEYVSCKSKLSIVETELLNLK 753

Query: 416  IVDYYQTHILKNTLQKLQRQITNAKVLEERWIQFFRKSLPNVILIMSPLKTQQEFIERVS 595
                 + H+ KN  Q+L       K+  E+          ++ ++++ L+T Q+  ER  
Sbjct: 754  EEQKLRVHLEKNLKQELN------KLSPEK---------DSLRIMVTQLQTLQK--ERED 796

Query: 596  LYEFKRR--LTKVEDLKKKLMTAKNDIS-KDWSVLQ 694
            L E  R+    K+++L+  L   K + S KD  + Q
Sbjct: 797  LLEETRKSCQKKIDELEDALSELKKETSQKDHHIKQ 832
>sp|Q04347|BUD22_YEAST Bud site selection protein 22
          Length = 519

 Score = 33.5 bits (75), Expect = 0.79
 Identities = 34/134 (25%), Positives = 69/134 (51%), Gaps = 14/134 (10%)
 Frame = +2

Query: 323 LTNALLGKNVKNLNEVLGCI------RQVDEQFAN--NNIVDYYQTHI---LKNTLQKLQ 469
           L N +  KN K + ++L  +      +++DE ++   NN + YY+TH+   +K  +QK+ 
Sbjct: 39  LYNCIGKKNKKKIEKLLNSLELKTLDKELDESYSKLLNNKIHYYETHLSKCIKEQIQKIS 98

Query: 470 RQITNAKVLEERWIQFFRKSLPNVILIMSPLKTQQEFIERVSLYEFKRRLTKV--EDLKK 643
           ++  ++KV +       +K+    I I   L TQ   ++ ++L+  + RL K+  + +K+
Sbjct: 99  KK-NSSKVKDA------QKNKSPSIDIEKMLATQLS-LDDLALFMTRFRLIKILHQRIKQ 150

Query: 644 KLMTAKNDI-SKDW 682
           K    + D  +K W
Sbjct: 151 KSKKIEGDTNNKTW 164
>sp|P31916|MAT2_EUGGR Maturase-like protein 2
          Length = 758

 Score = 32.3 bits (72), Expect = 1.8
 Identities = 18/53 (33%), Positives = 25/53 (47%)
 Frame = -3

Query: 229 KCSRIFTQLFLFYFQKKSIIAFFYRLIQTIRIIKSFTDFFINYRTIFCCISLI 71
           +C   F +     F KK   +FF    ++ RI+K F  FFIN    F  + LI
Sbjct: 453 RCFNFFKKNCYPNFYKKKNFSFFNFYTESFRILKFFDAFFINTHHFFLPVELI 505
>sp|Q9UZC8|RAD50_PYRAB DNA double-strand break repair rad50 ATPase
          Length = 880

 Score = 32.3 bits (72), Expect = 1.8
 Identities = 23/75 (30%), Positives = 42/75 (56%), Gaps = 3/75 (4%)
 Frame = +2

Query: 446 KNTLQKLQRQITNAKV-LEERWIQFFRKSLPNVILIMSPLKTQQEFIERVSLY--EFKRR 616
           +  L KL+R++++    LEE      +KS+  +   +  LK ++E  E+  L   + ++ 
Sbjct: 666 RERLVKLEREVSSLTARLEE-----LKKSVEQIKATLRKLKEEKEEREKAKLEIKKLEKA 720

Query: 617 LTKVEDLKKKLMTAK 661
           L+KVEDL+KK+   K
Sbjct: 721 LSKVEDLRKKIKDYK 735
>sp|P10499|KCNA1_RAT Potassium voltage-gated channel subfamily A member 1 (Voltage-gated
           potassium channel subunit Kv1.1) (IA) (RBKI) (RCK1)
          Length = 495

 Score = 32.0 bits (71), Expect = 2.3
 Identities = 20/57 (35%), Positives = 27/57 (47%)
 Frame = -3

Query: 223 SRIFTQLFLFYFQKKSIIAFFYRLIQTIRIIKSFTDFFINYRTIFCCISLIKYFIAI 53
           S IFT  F F  +   II F + L+       S TDFF N       +++I YFI +
Sbjct: 214 SNIFTDPF-FIVETLCIIWFSFELVVRFFACPSKTDFFKNIMNFIDIVAIIPYFITL 269
>sp|P16388|KCNA1_MOUSE Potassium voltage-gated channel subfamily A member 1 (Voltage-gated
           potassium channel subunit Kv1.1) (MKI) (MBK1)
          Length = 495

 Score = 32.0 bits (71), Expect = 2.3
 Identities = 20/57 (35%), Positives = 27/57 (47%)
 Frame = -3

Query: 223 SRIFTQLFLFYFQKKSIIAFFYRLIQTIRIIKSFTDFFINYRTIFCCISLIKYFIAI 53
           S IFT  F F  +   II F + L+       S TDFF N       +++I YFI +
Sbjct: 214 SNIFTDPF-FIVETLCIIWFSFELVVRFFACPSKTDFFKNIMNFIDIVAIIPYFITL 269
>sp|Q09470|KCNA1_HUMAN Potassium voltage-gated channel subfamily A member 1 (Voltage-gated
           potassium channel subunit Kv1.1) (HUKI) (HBK1)
          Length = 495

 Score = 32.0 bits (71), Expect = 2.3
 Identities = 20/57 (35%), Positives = 27/57 (47%)
 Frame = -3

Query: 223 SRIFTQLFLFYFQKKSIIAFFYRLIQTIRIIKSFTDFFINYRTIFCCISLIKYFIAI 53
           S IFT  F F  +   II F + L+       S TDFF N       +++I YFI +
Sbjct: 214 SNIFTDPF-FIVETLCIIWFSFELVVRFFACPSKTDFFKNIMNFIDIVAIIPYFITL 269
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
          Length = 1790

 Score = 32.0 bits (71), Expect = 2.3
 Identities = 33/138 (23%), Positives = 62/138 (44%), Gaps = 4/138 (2%)
 Frame = +2

Query: 266  NKLDLFSSKNENWQIVQEALTNALLGKNVKNLNEVLGCIRQVDEQF--ANNNIVDYYQTH 439
            NK+D  S + EN+QI + ++      KN++ L + +  + Q  E+    +++  D Y++ 
Sbjct: 1001 NKIDSMSQEKENFQIERGSIE-----KNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQ 1055

Query: 440  ILKNTLQKLQRQITNAKVLEERWIQFFRKSLPNVILIMSPLKTQQEFIERVSLYE-FKRR 616
            I       L+ ++  A    +           NV  I    KT++E    ++ Y+  K  
Sbjct: 1056 I-----SLLKEKLETATTANDE----------NVNKISELTKTREELEAELAAYKNLKNE 1100

Query: 617  L-TKVEDLKKKLMTAKND 667
            L TK+E  +K L   K +
Sbjct: 1101 LETKLETSEKALKEVKEN 1118
>sp|P49947|FRIM_SALSA Ferritin, middle subunit (Ferritin M)
          Length = 176

 Score = 32.0 bits (71), Expect = 2.3
 Identities = 24/94 (25%), Positives = 46/94 (48%), Gaps = 6/94 (6%)
 Frame = +2

Query: 221 RTFSDKVMSHSNTSLNKL---DLFSSKNENWQIVQEALTNALLGKNVKNLNEVLGCIRQV 391
           R  +DK++S  N    ++   D+   + + W    EA+  AL  +  KN+N+ L  + ++
Sbjct: 60  REHADKLLSFQNKRGGRILLQDIKKPERDEWGNGLEAMQCAL--QLEKNVNQALLDLHKI 117

Query: 392 DEQFANNNIVDYYQTHILK---NTLQKLQRQITN 484
                + ++ D+ +TH L      ++KL   ITN
Sbjct: 118 ASDKVDPHLCDFLETHYLNEQVEAIKKLGDHITN 151
>sp|O66834|RECN_AQUAE DNA repair protein recN (Recombination protein N)
          Length = 520

 Score = 30.8 bits (68), Expect = 5.1
 Identities = 23/97 (23%), Positives = 44/97 (45%), Gaps = 10/97 (10%)
 Frame = +2

Query: 215 YSRTFSDKVMSHSNTSLNKLDLFSSKNENWQIVQEALTNA----------LLGKNVKNLN 364
           YS  FS+ +   +N      +L++S  E    + E   N           L  K  K+  
Sbjct: 234 YSGKFSELIEKIANLEEEVYELYNSLKEEMPEISEEEVNEINEKLFRIQRLEEKYKKSFP 293

Query: 365 EVLGCIRQVDEQFANNNIVDYYQTHILKNTLQKLQRQ 475
           E+L  + ++ E+ +N N VD+ +   L+  ++KL+ +
Sbjct: 294 EILKEVEEIKEELSNLNSVDFKEEE-LREEVEKLREE 329
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,525,303
Number of Sequences: 369166
Number of extensions: 1551592
Number of successful extensions: 4519
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4372
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4519
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)