Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_J24
(826 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding ... 107 3e-23
sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding ... 98 2e-20
sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding ... 95 2e-19
sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding ... 92 1e-18
sp|P48809|RB27C_DROME Heterogeneous nuclear ribonucleoprote... 47 6e-05
sp|Q99383|NAB4_YEAST Nuclear polyadenylated RNA-binding pro... 47 8e-05
sp|P51991|ROA3_HUMAN Heterogeneous nuclear ribonucleoprotei... 45 2e-04
sp|Q8BG05|ROA3_MOUSE Heterogeneous nuclear ribonucleoprotei... 45 2e-04
sp|Q20870|DAZ1_CAEEL DAZ protein 1 45 2e-04
sp|Q96EP5|DAZP1_HUMAN DAZ-associated protein 1 (Deleted in ... 45 2e-04
>sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 466
Score = 107 bits (268), Expect = 3e-23
Identities = 93/321 (28%), Positives = 146/321 (45%), Gaps = 46/321 (14%)
Frame = +2
Query: 2 ANNGCDLRRFMQSFLLVEQD--LGNFYVLNDIFRYQDQTF-----KVEDVEEAPVVEHES 160
+NN LRRFMQ+F+L + FYV NDIFRYQD+ F + ++ E V E E
Sbjct: 99 SNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEE 158
Query: 161 KNE------DIHGEINSWNELSRNCELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEKDI 322
+ + D G +S + E + E+ P+ E +E ++ +I E+ +
Sbjct: 159 RQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVS-EIQEEKPEP 217
Query: 323 SSEIPVEIDSQQ-------DIGQKMEEMNIKEKSWAAI--------------------IN 421
E D+Q+ DI Q ++E +++ SWA++ +
Sbjct: 218 VLEETAPEDAQKSSSPAPADIAQTVQE-DLRTFSWASVTSKNLPPSGAVPVTGIPPHVVK 276
Query: 422 PMSSRPSXXXXXXXXXXXXXXXXXXXXINSNGDNMEKRKPR-----FNNGNVKTPSTLNY 586
+S+P + +R PR G+++ + +
Sbjct: 277 VPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRH 336
Query: 587 PDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHE 766
PD HQLF+GNLP + + ELKDFF + YG V ++RI N G LPNFGF+VF + E
Sbjct: 337 PDSHQLFIGNLPHEVDKSELKDFF-QSYGNVVELRI------NSGGKLPNFGFVVFDDSE 389
Query: 767 VVEEILKNKPIYYSAH-RLNV 826
V+++L N+PI + RLNV
Sbjct: 390 PVQKVLSNRPIMFRGEVRLNV 410
>sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 465
Score = 98.2 bits (243), Expect = 2e-20
Identities = 91/322 (28%), Positives = 145/322 (45%), Gaps = 47/322 (14%)
Frame = +2
Query: 2 ANNGCDLRRFMQSFLLVEQD--LGNFYVLNDIFRYQDQTF----------KVEDVEEAPV 145
+NN LRRFMQ+F+L + FYV NDIFRYQD+ F E+VEE
Sbjct: 99 SNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPE- 157
Query: 146 VEHESKNEDIHGEINSWNE--LSRNCELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEKD 319
E + E + + ++ + +S + E + E+ P+ E E ++ I ++ +
Sbjct: 158 -ERQQTPEVVPDDSGTFYDQTVSNDLEEHLEEPVVEPEPEPEPEPEPEPVS-DIQEDKPE 215
Query: 320 ISSEIPVEIDSQQ-------DIGQKMEEMNIKEKSWAAIIN---------PMSSRPSXXX 451
+ E D Q+ D+ E++ + SWA++ + P++ P
Sbjct: 216 AALEEAAPDDVQKSTSPAPADVAPAQEDL--RTFSWASVTSKNLPPSGAVPVTGTPPHVV 273
Query: 452 XXXXXXXXXXXXXXXXXINSNGDNMEK-RKPRFN---------------NGNVKTPSTLN 583
++ R+ R N G+V+ +
Sbjct: 274 KVPASQPRPESKPDSQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEPGDVEPRRMVR 333
Query: 584 YPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNH 763
+PD HQLF+GNLP + + ELKDFF + +G V ++RI N G LPNFGF+VF +
Sbjct: 334 HPDSHQLFIGNLPHEVDKSELKDFF-QNFGNVVELRI------NSGGKLPNFGFVVFDDS 386
Query: 764 EVVEEILKNKPIYY-SAHRLNV 826
E V+++L N+PI + A RLNV
Sbjct: 387 EPVQKVLSNRPIMFRGAVRLNV 408
>sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 95.1 bits (235), Expect = 2e-19
Identities = 88/317 (27%), Positives = 139/317 (43%), Gaps = 42/317 (13%)
Frame = +2
Query: 2 ANNGCDLRRFMQSFLLVEQDL--GNFYVLNDIFRYQDQTF---------KVEDVEEAPVV 148
+N+G R+FMQ+F+L + FYV ND+FRY+D+ F + ED E
Sbjct: 99 SNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQE 158
Query: 149 EHESKNEDIHGEINSW----NELSRNCELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEK 316
E + E + NS + ++ E E+ P+ E E + ++ EEK
Sbjct: 159 ERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQV--EEK 216
Query: 317 DISSEIPVEIDSQQDIGQKMEEMNIKEK-----SWAAIIN---PMSSR--PSXXXXXXXX 466
++ E++ + E +++ ++ SWA++ + P S S
Sbjct: 217 NLE-----ELEEKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPSHVKA 271
Query: 467 XXXXXXXXXXXXINSNGDNMEKRKPRFNNG----------------NVKTPSTLNYPDEH 598
+ S + +++PR G + + YPD H
Sbjct: 272 PVSQPRVEAKPEVQSQPPRVREQRpreRPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSH 331
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
QLFVGNLP ++ E+ELK+FF +G V ++RI T G LPNFGF+VF + E V+
Sbjct: 332 QLFVGNLPHDIDENELKEFFM-SFGNVVELRI---NTKGVGGKLPNFGFVVFDDSEPVQR 387
Query: 779 ILKNKPIYYSAH-RLNV 826
IL KPI + RLNV
Sbjct: 388 ILIAKPIMFRGEVRLNV 404
>sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 92.4 bits (228), Expect = 1e-18
Identities = 86/317 (27%), Positives = 137/317 (43%), Gaps = 42/317 (13%)
Frame = +2
Query: 2 ANNGCDLRRFMQSFLLVEQDL--GNFYVLNDIFRYQDQTF-------------KVEDVEE 136
+N+G R+FMQ+F+L + FYV ND+FRY+D+ F +VE+ +E
Sbjct: 99 SNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQE 158
Query: 137 APVVEHESKNEDIHGEINSWNELSRNCELNNEQIPQSPQLIEHEEGSEEFINYKIMNEEK 316
E E+ + + ++ E E+ P+ E E + ++ EEK
Sbjct: 159 DRQPSPEPVQENANSAYYDAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQV--EEK 216
Query: 317 DISSEIPVEIDSQQDIGQKMEEMNIKEK-----SWAAIIN---PMSSR--PSXXXXXXXX 466
+ E++ + E ++ ++ SWA++ + P S S
Sbjct: 217 HLE-----ELEEKSATPPPAEPASLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKA 271
Query: 467 XXXXXXXXXXXXINSNGDNMEKRKPRFNNG----------------NVKTPSTLNYPDEH 598
+ S + +++PR G + + YPD H
Sbjct: 272 PVSQPRVDAKPEVQSQPPRVREQRpreRPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSH 331
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
QLFVGNLP ++ E+ELK+FF +G V ++RI T G LPNFGF+VF + E V+
Sbjct: 332 QLFVGNLPHDIDENELKEFFM-SFGNVVELRI---NTKGVGGKLPNFGFVVFDDSEPVQR 387
Query: 779 ILKNKPIYYSAH-RLNV 826
IL KPI + RLNV
Sbjct: 388 ILIAKPIMFRGEVRLNV 404
>sp|P48809|RB27C_DROME Heterogeneous nuclear ribonucleoprotein 27C (Hrb27-C) (hnRNP 48)
(HRP48.1)
Length = 421
Score = 47.0 bits (110), Expect = 6e-05
Identities = 29/89 (32%), Positives = 47/89 (52%), Gaps = 3/89 (3%)
Frame = +2
Query: 569 PSTLNYPDE---HQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNF 739
P TL P + +++F+G LP N+TE +L+ FF+ +YG V +V I + E K F
Sbjct: 84 PRTLQKPKKGGGYKVFLGGLPSNVTETDLRTFFN-RYGKVTEVVIMYDQ---EKKKSRGF 139
Query: 740 GFLVFHNHEVVEEILKNKPIYYSAHRLNV 826
GFL F VE + + I + ++ +
Sbjct: 140 GFLSFEEESSVEHVTNERYINLNGKQVEI 168
Score = 35.0 bits (79), Expect = 0.25
Identities = 21/66 (31%), Positives = 34/66 (51%)
Frame = +2
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
+LFVG L T++ L +F ++G + D + K+ NE FGF+ F + V
Sbjct: 8 KLFVGGLSWETTQENLSRYFC-RFGDIIDCVVMKN---NESGRSRGFGFVTFADPTNVNH 63
Query: 779 ILKNKP 796
+L+N P
Sbjct: 64 VLQNGP 69
>sp|Q99383|NAB4_YEAST Nuclear polyadenylated RNA-binding protein 4
Length = 534
Score = 46.6 bits (109), Expect = 8e-05
Identities = 24/67 (35%), Positives = 39/67 (58%)
Frame = +2
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
++F+G L + TED L+++F KYG V D++I K + + FGFL F V+E
Sbjct: 160 KMFIGGLNWDTTEDNLREYFG-KYGTVTDLKIMKDPATGRSR---GFGFLSFEKPSSVDE 215
Query: 779 ILKNKPI 799
++K + I
Sbjct: 216 VVKTQHI 222
Score = 37.0 bits (84), Expect = 0.066
Identities = 16/76 (21%), Positives = 43/76 (56%)
Frame = +2
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
++FVG + ++ E ++FFS+ +G + D ++ + + + + FGF+ + + + V+
Sbjct: 244 KIFVGGIGPDVRPKEFEEFFSQ-WGTIIDAQLMLDKDTGQSR---GFGFVTYDSADAVDR 299
Query: 779 ILKNKPIYYSAHRLNV 826
+ +NK I + ++ +
Sbjct: 300 VCQNKFIDFKDRKIEI 315
>sp|P51991|ROA3_HUMAN Heterogeneous nuclear ribonucleoprotein A3 (hnRNP A3)
Length = 378
Score = 45.4 bits (106), Expect = 2e-04
Identities = 22/76 (28%), Positives = 43/76 (56%)
Frame = +2
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
++FVG + ++ E L+D+F EKYG ++ + + + R S + + F F+ F +H+ V++
Sbjct: 127 KIFVGGIKEDTEEYNLRDYF-EKYGKIETIEVMEDRQSGKKR---GFAFVTFDDHDTVDK 182
Query: 779 ILKNKPIYYSAHRLNV 826
I+ K + H V
Sbjct: 183 IVVQKYHTINGHNCEV 198
Score = 33.5 bits (75), Expect = 0.73
Identities = 25/94 (26%), Positives = 45/94 (47%), Gaps = 1/94 (1%)
Frame = +2
Query: 518 DNMEKRKPRFNNGN-VKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRI 694
D+ +R+ R G+ K P L +LF+G L T+D L++ F EK+G + D +
Sbjct: 13 DSGRRRRRRGEEGHDPKEPEQLR-----KLFIGGLSFETTDDSLREHF-EKWGTLTDCVV 66
Query: 695 QKSRTSNEGKPLPNFGFLVFHNHEVVEEILKNKP 796
+ + + FGF+ + E V+ + +P
Sbjct: 67 MRDPQTKRSR---GFGFVTYSCVEEVDAAMCARP 97
>sp|Q8BG05|ROA3_MOUSE Heterogeneous nuclear ribonucleoprotein A3 (hnRNP A3)
sp|Q6URK4|ROA3_RAT Heterogeneous nuclear ribonucleoprotein A3 (hnRNP A3)
Length = 379
Score = 45.4 bits (106), Expect = 2e-04
Identities = 22/76 (28%), Positives = 43/76 (56%)
Frame = +2
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
++FVG + ++ E L+D+F EKYG ++ + + + R S + + F F+ F +H+ V++
Sbjct: 127 KIFVGGIKEDTEEYNLRDYF-EKYGKIETIEVMEDRQSGKKR---GFAFVTFDDHDTVDK 182
Query: 779 ILKNKPIYYSAHRLNV 826
I+ K + H V
Sbjct: 183 IVVQKYHTINGHNCEV 198
Score = 33.5 bits (75), Expect = 0.73
Identities = 25/94 (26%), Positives = 45/94 (47%), Gaps = 1/94 (1%)
Frame = +2
Query: 518 DNMEKRKPRFNNGN-VKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRI 694
D+ +R+ R G+ K P L +LF+G L T+D L++ F EK+G + D +
Sbjct: 13 DSGRRRRRRGEEGHDPKEPEQLR-----KLFIGGLSFETTDDSLREHF-EKWGTLTDCVV 66
Query: 695 QKSRTSNEGKPLPNFGFLVFHNHEVVEEILKNKP 796
+ + + FGF+ + E V+ + +P
Sbjct: 67 MRDPQTKRSR---GFGFVTYSCVEEVDAAMCARP 97
>sp|Q20870|DAZ1_CAEEL DAZ protein 1
Length = 499
Score = 45.4 bits (106), Expect = 2e-04
Identities = 27/79 (34%), Positives = 44/79 (55%), Gaps = 2/79 (2%)
Frame = +2
Query: 596 HQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVE 775
+++FVG P + TE EL++ F EK+ VKDV++ KS +GF+ F + E
Sbjct: 67 NRIFVGGFPTSTTETELREHF-EKFFAVKDVKMVKSLDGQS----KGYGFITFETEDQAE 121
Query: 776 EILKNKP--IYYSAHRLNV 826
EI K P + + + +LN+
Sbjct: 122 EIRKLTPKQLEFRSRKLNL 140
>sp|Q96EP5|DAZP1_HUMAN DAZ-associated protein 1 (Deleted in azoospermia-associated protein
1)
Length = 407
Score = 45.1 bits (105), Expect = 2e-04
Identities = 24/66 (36%), Positives = 40/66 (60%)
Frame = +2
Query: 599 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 778
+LFVG L + T++ L+ +FS+ YG V D I K +T+N+ + FGF+ F + V
Sbjct: 11 KLFVGGLDWSTTQETLRSYFSQ-YGEVVDCVIMKDKTTNQSR---GFGFVKFKDPNCVGT 66
Query: 779 ILKNKP 796
+L ++P
Sbjct: 67 VLASRP 72
Score = 37.7 bits (86), Expect = 0.039
Identities = 24/85 (28%), Positives = 44/85 (51%)
Frame = +2
Query: 524 MEKRKPRFNNGNVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKS 703
M+ + R G K P + N +++FVG +P N E EL+++F +K+G V +V +
Sbjct: 90 MQPERTRPKEGWQKGPRSDN-SKSNKIFVGGIPHNCGETELREYF-KKFGVVTEVVMIYD 147
Query: 704 RTSNEGKPLPNFGFLVFHNHEVVEE 778
+ FGF+ F + + V++
Sbjct: 148 AEKQRPR---GFGFITFEDEQSVDQ 169
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,054,608
Number of Sequences: 369166
Number of extensions: 1515345
Number of successful extensions: 5708
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5375
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5675
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7956112725
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)