Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_J14
(511 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P53073|YGY1_YEAST Hypothetical 21.5 kDa protein in SEC15... 80 4e-15
sp|O51314|RL13_BORBU 50S ribosomal protein L13 30 4.3
sp|P00548|KPYK_CHICK Pyruvate kinase, muscle isozyme 29 5.6
sp|Q5ZKK1|MARE2_CHICK Microtubule-associated protein RP/EB ... 29 5.6
sp|P16453|DCHS_RAT Histidine decarboxylase (HDC) 29 7.3
sp|Q9UIS9|MBD1_HUMAN Methyl-CpG-binding domain protein 1 (M... 29 7.3
sp|P14680|YAK1_YEAST Protein kinase YAK1 28 9.6
>sp|P53073|YGY1_YEAST Hypothetical 21.5 kDa protein in SEC15-SAP4 intergenic region
Length = 190
Score = 79.7 bits (195), Expect = 4e-15
Identities = 46/144 (31%), Positives = 72/144 (50%), Gaps = 20/144 (13%)
Frame = +2
Query: 131 LPSPVGYSDRSLPSSSVRKNDSD---------------LMVKRSWDIALGPIKQIPMNLI 265
LPSP G+ S + RK L V+++W IAL P K IPMN+
Sbjct: 30 LPSPPGFEGNSSKGNVTRKQQDATSQTTSLAQKNQITVLQVQKAWQIALQPAKSIPMNIF 89
Query: 266 LMWMAGNTISIFPIVMVFMMFLRPIQAIFSMQSTFQVIEGSAAS-----IQCFVYFLGNV 430
+ +M+G ++ I PI+ M+ PI+AIFS +S F+ + G+ A+ F+Y +
Sbjct: 90 MSYMSGTSLQIIPIMTALMLLSGPIKAIFSTRSAFKPVLGNKATQSQVQTAMFMYIVFQG 149
Query: 431 LNLGLAVYKCHIMGLLPTFASDWL 502
+ + + K + MGL+P DWL
Sbjct: 150 VLMYIGYRKLNSMGLIPNAKGDWL 173
>sp|O51314|RL13_BORBU 50S ribosomal protein L13
Length = 146
Score = 29.6 bits (65), Expect = 4.3
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +3
Query: 48 YQKLRENGILISVAKVKQTDHNYHHRLIYLH 140
+Q L +N I+I+ +KVK T Y +L Y H
Sbjct: 53 HQDLGDNVIIINASKVKLTGKKYQQKLYYRH 83
>sp|P00548|KPYK_CHICK Pyruvate kinase, muscle isozyme
Length = 530
Score = 29.3 bits (64), Expect = 5.6
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = -2
Query: 363 DCMENIAWIGRKNIIKTITMGN 298
+C EN+ W+ KN+IK I +G+
Sbjct: 150 NCDENVLWVDYKNLIKVIDVGS 171
>sp|Q5ZKK1|MARE2_CHICK Microtubule-associated protein RP/EB family member 2
Length = 338
Score = 29.3 bits (64), Expect = 5.6
Identities = 19/56 (33%), Positives = 28/56 (50%)
Frame = +2
Query: 35 QPVDISKASRKWNFDFSGKSKADRSQLSSPVDLPSPVGYSDRSLPSSSVRKNDSDL 202
Q ++ K S N +G +K+ S S P PS + ++ PSSS K+D DL
Sbjct: 187 QIFNLPKKSHHANSPTAGAAKS--SPASKPGSTPSRPSSAKKAAPSSSASKSDKDL 240
>sp|P16453|DCHS_RAT Histidine decarboxylase (HDC)
Length = 656
Score = 28.9 bits (63), Expect = 7.3
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +2
Query: 146 GYSDRSLPSSSVRKNDSDLMVKRSWDIALGPIKQIPMNLILMWMAGNTISIFP 304
GY +PSS+ + DS WD G I+QI M ++ W + + + +P
Sbjct: 40 GYLRAQIPSSAPEEPDS-------WDSIFGDIEQIIMPGVVHWQSPHMHAYYP 85
>sp|Q9UIS9|MBD1_HUMAN Methyl-CpG-binding domain protein 1 (Methyl-CpG binding protein
MBD1) (Protein containing methyl-CpG-binding domain 1)
Length = 605
Score = 28.9 bits (63), Expect = 7.3
Identities = 19/70 (27%), Positives = 29/70 (41%)
Frame = +2
Query: 104 RSQLSSPVDLPSPVGYSDRSLPSSSVRKNDSDLMVKRSWDIALGPIKQIPMNLILMWMAG 283
R SSPV +P PV S +L + SW +AL +KQ + W G
Sbjct: 460 REGASSPVQVPGPVAASTEALLQEAQCSG-------LSWVVALPQVKQEKADTQDEWTPG 512
Query: 284 NTISIFPIVM 313
+ P+++
Sbjct: 513 TAVLTSPVLV 522
>sp|P14680|YAK1_YEAST Protein kinase YAK1
Length = 807
Score = 28.5 bits (62), Expect = 9.6
Identities = 11/29 (37%), Positives = 21/29 (72%)
Frame = +2
Query: 353 SMQSTFQVIEGSAASIQCFVYFLGNVLNL 439
S+Q++ ++I+ + +C ++FLG VLNL
Sbjct: 659 SIQNSQELIDQEMQNRECLIHFLGGVLNL 687
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,163,290
Number of Sequences: 369166
Number of extensions: 1331398
Number of successful extensions: 3466
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 3360
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3459
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3255600150
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)