Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_H16
(609 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 99 6e-21
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 88 1e-17
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 86 7e-17
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 86 1e-16
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 84 4e-16
sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precurs... 81 2e-15
sp|P55113|NAS7_CAEEL Zinc metalloproteinase nas-7 precursor... 81 2e-15
sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor... 81 2e-15
sp|Q6HA08|ASTL_HUMAN Astacin-like metalloendopeptidase prec... 80 3e-15
sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precurs... 79 7e-15
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 99.4 bits (246), Expect = 6e-21
Identities = 54/153 (35%), Positives = 88/153 (57%), Gaps = 5/153 (3%)
Frame = +3
Query: 165 KRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASHYIEVQSL--DQG 338
+RWPNN++ ++ + + + + ++ + E CVKF S + + + D+G
Sbjct: 102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHPDEG 159
Query: 339 CYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRIL 518
CYSL+G TGG KQP++L + GC+ GT+ HE +HA+GF H R+DRD++I+V W ++
Sbjct: 160 CYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVM 216
Query: 519 TSHCSQFVKCD---GCEVDGPYETHSVMHYPSY 608
QF K + +D PY+ S+MHY Y
Sbjct: 217 NGADDQFEKYNLNVISHLDEPYDYASIMHYGPY 249
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 88.2 bits (217), Expect = 1e-17
Identities = 59/185 (31%), Positives = 95/185 (51%), Gaps = 11/185 (5%)
Frame = +3
Query: 78 LNNMQM-----GDMVLDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPNDKK 227
+NN M GD+VL K+ A+ F A ++ RWP + +V + + + +D+K
Sbjct: 52 MNNNSMEELLEGDLVLPKTRNAM-KCFGA--PDSCRWPKSSNGIVKVPYVVSDNYESDEK 108
Query: 228 GIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGC 404
+R + E + C+ F ++ Y+ ++ GC S++GY G KQ + LQ GC
Sbjct: 109 ETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD-KQVVVLQRFGC 164
Query: 405 MYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETH 584
+ ++HE +HALGF H H R DRD H+ + W+ I+ F K D + PY+
Sbjct: 165 I-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYG 223
Query: 585 SVMHY 599
S+MHY
Sbjct: 224 SIMHY 228
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 85.9 bits (211), Expect = 7e-17
Identities = 45/109 (41%), Positives = 65/109 (59%), Gaps = 1/109 (0%)
Frame = +3
Query: 276 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 452
C++F + + +I V S + GCYS LG GG +Q L+L GCMYS G ++HE HALG
Sbjct: 129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALG 185
Query: 453 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHY 599
F H R DRD+++ + W I+ + F K D ++ PY+ S+MHY
Sbjct: 186 FQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHY 234
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 85.5 bits (210), Expect = 1e-16
Identities = 44/109 (40%), Positives = 65/109 (59%), Gaps = 1/109 (0%)
Frame = +3
Query: 276 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 452
C++F + + +I V S GCYS LG GG +Q L++ GCMYS G ++HE HALG
Sbjct: 120 CIRFVRRTNEYDFISVVS-KTGCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALG 176
Query: 453 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHY 599
F H R DRD+++ + W+ I+ + F K D ++ PY+ S+MHY
Sbjct: 177 FQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHY 225
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 83.6 bits (205), Expect = 4e-16
Identities = 48/148 (32%), Positives = 81/148 (54%), Gaps = 2/148 (1%)
Frame = +3
Query: 171 WPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYS 347
W + ++FA D+ I+ + EL++ C++F ++ S Y+E+ + GC+S
Sbjct: 59 WSGGVIPYTFAGVSGADQSAILSG-MQELEEK--TCIRFVPRTTESDYVEIFTSGSGCWS 115
Query: 348 LLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSH 527
+G G +Q ++LQ GC+Y GT+ HE +HA+GF H H R DRDN++ + + + S
Sbjct: 116 YVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSM 173
Query: 528 CSQF-VKCDGCEVDGPYETHSVMHYPSY 608
S F + V Y+ +S+MHY Y
Sbjct: 174 TSNFDIDTYSRYVGEDYQYYSIMHYGKY 201
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
Length = 527
Score = 81.3 bits (199), Expect = 2e-15
Identities = 50/149 (33%), Positives = 78/149 (52%), Gaps = 5/149 (3%)
Frame = +3
Query: 168 RWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASH--YIEVQSLDQGC 341
+W ++ ++ + + + + + E + E +K C+ FS SA YI + D GC
Sbjct: 195 KWEQARIPYTISSQYSSYSRSKIAEAIEEYRKK--TCIDFSPKSAGDLDYIHIVP-DDGC 251
Query: 342 YSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILT 521
YSL+G GG KQP++L + GC+ KG + HE +HA+GF H R DRD ++ + W +
Sbjct: 252 YSLVGRIGG-KQPVSLGD-GCI-QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEA 308
Query: 522 SHCSQFVKCDGCEVD---GPYETHSVMHY 599
QF K +D Y+ SVMHY
Sbjct: 309 GLQDQFDKYSLNMIDHLGTKYDYGSVMHY 337
>sp|P55113|NAS7_CAEEL Zinc metalloproteinase nas-7 precursor (Nematode astacin 7)
Length = 382
Score = 81.3 bits (199), Expect = 2e-15
Identities = 54/171 (31%), Positives = 89/171 (52%), Gaps = 7/171 (4%)
Frame = +3
Query: 114 KSDIALINGFKAHYSN--TKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF 287
KSDI L K + + K WPN ++ ++ +P + ++ ++ + + + + C++F
Sbjct: 68 KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHEK--TCIRF 125
Query: 288 --SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMH 461
++ Y+ + +D GC+S +G T G Q L+L N GCM T+ HE +H +GF H
Sbjct: 126 VPRQTGEPDYLFIGKVD-GCFSEVGRTSGV-QVLSLDN-GCM-EYATIIHEMMHVVGFYH 181
Query: 462 THMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVD---GPYETHSVMHYPS 605
H R DRDN I++ W I QF K D + PY+ S++HY S
Sbjct: 182 EHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDS 232
>sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor (Nematode astacin 8)
Length = 403
Score = 80.9 bits (198), Expect = 2e-15
Identities = 54/155 (34%), Positives = 82/155 (52%), Gaps = 5/155 (3%)
Frame = +3
Query: 156 SNTKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSA--SHYIEVQSL 329
+ T++WPN ++ + + + ND++ V + D CV+F +A + Y+ + +
Sbjct: 116 TGTRKWPNGRIPYVISNQY-NDRERAVLARSFQAYHDK-TCVRFVPRTAVDNDYLYIGKI 173
Query: 330 DQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWD 509
D GCYS +G GG +Q L+L N GC+ T HE +H++GF H H R DRD HI + W
Sbjct: 174 D-GCYSDVGRAGG-RQELSLDN-GCL-QYDTAIHELMHSVGFYHEHERWDRDEHITILWH 229
Query: 510 RILTSHCSQFVKCDGCE---VDGPYETHSVMHYPS 605
I QF K D E Y+ +S+MHY S
Sbjct: 230 NIDREAYDQFGKVDLAESSYYGQLYDYYSIMHYDS 264
>sp|Q6HA08|ASTL_HUMAN Astacin-like metalloendopeptidase precursor (Oocyte astacin)
(Ovastacin)
Length = 431
Score = 80.5 bits (197), Expect = 3e-15
Identities = 50/170 (29%), Positives = 82/170 (48%), Gaps = 6/170 (3%)
Frame = +3
Query: 108 LDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPNDKKGIVRECLVELQKDLG 272
L + DI + F+ + + +WP +V F + + + ++ E L E ++
Sbjct: 73 LIEGDIIRPSPFRLLSAASNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS-- 130
Query: 273 NCVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHAL 449
C++F + +I + + GC+S +G +GG Q ++L +G V HE +H L
Sbjct: 131 TCIRFVTYQDQRDFISIIPM-YGCFSSVGRSGG-MQVVSLAPTCLQKGRGIVLHELMHVL 188
Query: 450 GFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHY 599
GF H H R DRD +I V W+ IL F+K + PY+ SVMHY
Sbjct: 189 GFWHEHTRADRDRYIRVNWNEILPGFEINFIKSRSSNMLTPYDYSSVMHY 238
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
Length = 571
Score = 79.3 bits (194), Expect = 7e-15
Identities = 46/158 (29%), Positives = 83/158 (52%), Gaps = 5/158 (3%)
Frame = +3
Query: 141 FKAHYSNTKRWPNNQVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKF--SESSASHYI 314
+ A + + WP ++ ++ + + + + ++ + E C+++ E++ +Y+
Sbjct: 113 YNAIKNRLQLWPEGRIPYTISSQYSSYSRSLIAASMQEYASH--TCIRWVPKEAADVNYV 170
Query: 315 EVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHI 494
+ D+GCYS++G GG KQ L+L + GC+ KG + HE +HA+GF H R DRD+HI
Sbjct: 171 HIYP-DRGCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAVGFFHEQSRTDRDDHI 226
Query: 495 NVKWDRILTSHCSQFVKCDGCEVDG---PYETHSVMHY 599
+ W+ I QF K + Y+ S+MHY
Sbjct: 227 TIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHY 264
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,120,111
Number of Sequences: 369166
Number of extensions: 1648856
Number of successful extensions: 4004
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3860
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3964
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4748907085
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)