Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_H08
(479 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q7Z7M1|GP144_HUMAN Probable G-protein coupled receptor 1... 33 0.34
sp|P26363|CFTR_XENLA Cystic fibrosis transmembrane conducta... 32 0.75
sp|P26362|CFTR_SQUAC Cystic fibrosis transmembrane conducta... 32 0.98
sp|Q5D1Z7|CFTR_TRIVU Cystic fibrosis transmembrane conducta... 31 1.3
sp|Q14686|NCOA6_HUMAN Nuclear receptor coactivator 6 (Ampli... 31 1.7
sp|Q15038|DAZP2_HUMAN DAZ-associated protein 2 (Deleted in ... 30 2.2
sp|P49791|NU153_RAT Nuclear pore complex protein Nup153 (Nu... 30 2.2
sp|Q9DCP9|DAZP2_MOUSE DAZ-associated protein 2 (Deleted in ... 30 2.2
sp|P83509|GRLF1_CANFA Glucocorticoid receptor DNA-binding f... 30 2.2
sp|P19212|NIT2_NEUCR Nitrogen catabolic enzyme regulatory p... 30 2.9
>sp|Q7Z7M1|GP144_HUMAN Probable G-protein coupled receptor 144 (G-protein coupled receptor
PGR24)
Length = 963
Score = 33.1 bits (74), Expect = 0.34
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
Frame = +1
Query: 121 PNAGAPAYQSNLMQSDFQSQ--GYAMSGNNWMVPGQMNANSAFGVQHNPFFNNSVFPGQP 294
P A A + ++N +Q +Q +S W V G++N F +QH + +FP P
Sbjct: 548 PQAPASSEEANRVQRFLSTQVGSAIISSEVWDVTGEVNVAMTFHLQHRA--QSPLFPPHP 605
Query: 295 AIPISQPAFPTSG 333
P + A+ T+G
Sbjct: 606 PSPYTGGAWATTG 618
>sp|P26363|CFTR_XENLA Cystic fibrosis transmembrane conductance regulator (CFTR)
(cAMP-dependent chloride channel)
Length = 1485
Score = 32.0 bits (71), Expect = 0.75
Identities = 19/82 (23%), Positives = 38/82 (46%)
Frame = +1
Query: 19 NISGKLDKSSILALYNNSSSTMNTMGQMPNSTTWPNAGAPAYQSNLMQSDFQSQGYAMSG 198
NI+ K++K +LA+ ++ + ++ M P+AG + + S S
Sbjct: 444 NINFKIEKGQLLAIAGSTGAGKTSLLMMIMGELEPSAGKIKHSGRISFSPQVS------- 496
Query: 199 NNWMVPGQMNANSAFGVQHNPF 264
W++PG + N FGV ++ +
Sbjct: 497 --WIMPGTIKENIVFGVSYDQY 516
>sp|P26362|CFTR_SQUAC Cystic fibrosis transmembrane conductance regulator (CFTR)
(cAMP-dependent chloride channel)
Length = 1492
Score = 31.6 bits (70), Expect = 0.98
Identities = 21/89 (23%), Positives = 40/89 (44%)
Frame = +1
Query: 13 IPNISGKLDKSSILALYNNSSSTMNTMGQMPNSTTWPNAGAPAYQSNLMQSDFQSQGYAM 192
+ NIS KL+K +LA+ ++ S +++ M P+ G + + S
Sbjct: 442 LKNISFKLEKGELLAIAGSTGSGKSSLLMMIMGELEPSDGKIKHSGRISYSPQVP----- 496
Query: 193 SGNNWMVPGQMNANSAFGVQHNPFFNNSV 279
W++PG + N FG+ ++ + SV
Sbjct: 497 ----WIMPGTIKDNIIFGLSYDEYRYTSV 521
>sp|Q5D1Z7|CFTR_TRIVU Cystic fibrosis transmembrane conductance regulator (CFTR)
(cAMP-dependent chloride channel)
Length = 1478
Score = 31.2 bits (69), Expect = 1.3
Identities = 21/89 (23%), Positives = 42/89 (47%)
Frame = +1
Query: 13 IPNISGKLDKSSILALYNNSSSTMNTMGQMPNSTTWPNAGAPAYQSNLMQSDFQSQGYAM 192
+ NIS K++K +LA+ ++ + ++ + P+ G + + F SQ
Sbjct: 441 LKNISFKIEKGQLLAVAGSTGAGKTSLLMLIMGELEPSEGKIKHSGRI---SFCSQF--- 494
Query: 193 SGNNWMVPGQMNANSAFGVQHNPFFNNSV 279
+W++PG + N FGV ++ + SV
Sbjct: 495 ---SWIMPGTIKENIIFGVSYDEYRYRSV 520
>sp|Q14686|NCOA6_HUMAN Nuclear receptor coactivator 6 (Amplified in breast cancer-3
protein) (Cancer-amplified transcriptional coactivator
ASC-2) (Activating signal cointegrator-2) (ASC-2)
(Peroxisome proliferator-activated receptor-interacting
protein) (PPAR-interacting protein) (PRIP) (Nuclear
receptor-activating protein, 250 kDa) (Nuclear receptor
coactivator RAP250) (NRC RAP250) (Thyroid hormone
receptor-binding protein)
Length = 2063
Score = 30.8 bits (68), Expect = 1.7
Identities = 33/119 (27%), Positives = 41/119 (34%), Gaps = 30/119 (25%)
Frame = +1
Query: 94 GQMPNST--TWPNAGAPAYQSNLMQSDFQSQGYAMSGNNWMV---PGQMNANSAFGVQHN 258
GQ+P++T T N+GAP Q+N QG N V P M S G+ N
Sbjct: 531 GQVPSTTATTPGNSGAPQLQANQNVQHAGGQGAGPPQNQMQVSHGPPNMMQPSLMGIHGN 590
Query: 259 -----------PFFNNSVFPGQP--------------AIPISQPAFPTSGLTQPQNPML 360
P N S GQP +P G PQNPM+
Sbjct: 591 MNNQQAGTSGVPQVNLSNMQGQPQQGPPSQLMGMHQQIVPSQGQMVQQQGTLNPQNPMI 649
>sp|Q15038|DAZP2_HUMAN DAZ-associated protein 2 (Deleted in azoospermia-associated protein
2)
Length = 168
Score = 30.4 bits (67), Expect = 2.2
Identities = 25/90 (27%), Positives = 32/90 (35%), Gaps = 2/90 (2%)
Frame = +1
Query: 82 MNTMGQMPNSTTWP--NAGAPAYQSNLMQSDFQSQGYAMSGNNWMVPGQMNANSAFGVQH 255
MN+ GQ P T+P G P Y L P +A A+ +
Sbjct: 1 MNSKGQYPTQPTYPVQPPGNPVYPQTLHLPQ--------------APPYTDAPPAYSELY 46
Query: 256 NPFFNNSVFPGQPAIPISQPAFPTSGLTQP 345
P F V PG +P AFP + L P
Sbjct: 47 RPSF---VHPGAATVPTMSAAFPGASLYLP 73
>sp|P49791|NU153_RAT Nuclear pore complex protein Nup153 (Nucleoporin Nup153) (153 kDa
nucleoporin)
Length = 1468
Score = 30.4 bits (67), Expect = 2.2
Identities = 30/122 (24%), Positives = 51/122 (41%)
Frame = +1
Query: 4 SSAIPNISGKLDKSSILALYNNSSSTMNTMGQMPNSTTWPNAGAPAYQSNLMQSDFQSQG 183
SS+ P S + K S+ + +T G N+TT A PA+ + + S S
Sbjct: 1138 SSSKPTFSFSVAKPSVKESDQLAKATF-AFGNQTNTTTDQGAAKPAF--SFLNSSSSSSS 1194
Query: 184 YAMSGNNWMVPGQMNANSAFGVQHNPFFNNSVFPGQPAIPISQPAFPTSGLTQPQNPMLW 363
+ ++ + G ++S+ V F GQ + P+S AF S + P+L+
Sbjct: 1195 TPATSSSASIFGSSTSSSSPPVAAFVF-------GQASNPVSSSAFGNSAESSTSQPLLF 1247
Query: 364 *Q 369
Q
Sbjct: 1248 PQ 1249
>sp|Q9DCP9|DAZP2_MOUSE DAZ-associated protein 2 (Deleted in azoospermia-associated protein
2) (Proline-rich protein expressed in brain)
Length = 168
Score = 30.4 bits (67), Expect = 2.2
Identities = 25/90 (27%), Positives = 32/90 (35%), Gaps = 2/90 (2%)
Frame = +1
Query: 82 MNTMGQMPNSTTWP--NAGAPAYQSNLMQSDFQSQGYAMSGNNWMVPGQMNANSAFGVQH 255
MN+ GQ P T+P G P Y L P +A A+ +
Sbjct: 1 MNSKGQYPTQPTYPVQPPGNPVYPQTLHLPQ--------------APPYTDAPPAYSELY 46
Query: 256 NPFFNNSVFPGQPAIPISQPAFPTSGLTQP 345
P F V PG +P AFP + L P
Sbjct: 47 RPSF---VHPGAATVPTMSAAFPGASLYLP 73
>sp|P83509|GRLF1_CANFA Glucocorticoid receptor DNA-binding factor 1 (Rho GAP p190A) (p190-A)
Length = 1500
Score = 30.4 bits (67), Expect = 2.2
Identities = 21/84 (25%), Positives = 40/84 (47%), Gaps = 5/84 (5%)
Frame = +1
Query: 16 PNISGKLDKSSILA---LYNNSSSTMNTMGQMPNSTTWPNAGAPAYQSNLMQS--DFQSQ 180
P +DK +I+ +Y+N++ +T ++ NS P AG+P SNL S D +
Sbjct: 936 PFFKDVVDKKNIIEATHMYDNAAEACSTTEEVFNS---PRAGSPLCNSNLQDSEEDIEPP 992
Query: 181 GYAMSGNNWMVPGQMNANSAFGVQ 252
Y++ + +P +S ++
Sbjct: 993 SYSLFREDTSLPSLSKDHSKLSME 1016
>sp|P19212|NIT2_NEUCR Nitrogen catabolic enzyme regulatory protein
Length = 1036
Score = 30.0 bits (66), Expect = 2.9
Identities = 22/88 (25%), Positives = 38/88 (43%), Gaps = 3/88 (3%)
Frame = +1
Query: 88 TMGQMPNSTTWPNAGAPAYQS-NLMQSDFQSQGYAMSGNNWMVPGQMNA--NSAFGVQHN 258
T + N+ W +G P QS + QSD + + + + + +MN A H+
Sbjct: 636 TTDYVDNTGEWDGSGLPRSQSQSFRQSDLRKGKMSRTASTPGLSARMNPFERLAQSASHS 695
Query: 259 PFFNNSVFPGQPAIPISQPAFPTSGLTQ 342
P + G ++P S+P+ P G Q
Sbjct: 696 PPADVGRSSGLSSVPASRPSSPPPGAKQ 723
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,456,402
Number of Sequences: 369166
Number of extensions: 1319497
Number of successful extensions: 3108
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3102
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2822184570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)