Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_H04
(858 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q96PK2|MACF4_HUMAN Microtubule-actin crosslinking factor... 40 0.006
sp|O33600|RAD50_SULAC DNA double-strand break repair rad50 ... 34 0.60
sp|Q92614|MY18A_HUMAN Myosin-18A (Myosin XVIIIa) (Myosin co... 33 0.78
sp|P36130|YK16_YEAST Hypothetical 74.7 kDa Trp-Asp repeats ... 33 1.0
sp|O15083|ERC2_HUMAN ERC protein 2 33 1.3
sp|P47540|P115_MYCGE Protein P115 homolog 33 1.3
sp|P08928|LAM0_DROME Lamin Dm0 33 1.3
sp|Q8K3M6|ERC2_RAT ERC protein 2 (CAZ-associated structural... 33 1.3
sp|Q60HE6|MRE11_MACFA Double-strand break repair protein MR... 33 1.3
sp|O29230|RAD50_ARCFU DNA double-strand break repair rad50 ... 33 1.3
>sp|Q96PK2|MACF4_HUMAN Microtubule-actin crosslinking factor 1, isoform 4
Length = 5938
Score = 40.4 bits (93), Expect = 0.006
Identities = 46/221 (20%), Positives = 100/221 (45%), Gaps = 12/221 (5%)
Frame = +3
Query: 93 AERKAATDKQMALVKQMHIDQLMSEITEKNSNIATMSRVLNENMKSHKTVADKSVREVDG 272
AERKA D +A Q I + +++ + + M R+LNE +S + + +++ +VD
Sbjct: 1974 AERKAQLDA-LAFDIQFFISEHAQDLSPQQNR--QMLRLLNELQRSFQDILEQTAAQVDA 2030
Query: 273 LKKSLREE---------RKMKQSAFTKVDDLISQVYEFEAAFAIVKGSTPVNGVNS-KTD 422
L+ L++ +K + + +++DL S V + E A A +G T +++ + +
Sbjct: 2031 LQGHLQQMEQEALVKTLQKQQNTCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKN 2090
Query: 423 SISCSQISRPSTGVGGNLKSGLKPITPDMKISSTQTRPRPITYGDILRRYPIPYAPDHYQ 602
+ + + +K I M+ + T+ PR +T R + A + Y+
Sbjct: 2091 QSDLKDLQDDIQNRATSFATVVKDIEGFMEENQTKLSpreLT----ALREKLHQAKEQYE 2146
Query: 603 EFRRD-RVGAHQLRKRIADQLLMNLE-PETHEQLSKSNKAL 719
+ + RV +L + + L E + ++L+++ K +
Sbjct: 2147 ALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKI 2187
>sp|O33600|RAD50_SULAC DNA double-strand break repair rad50 ATPase
Length = 886
Score = 33.9 bits (76), Expect = 0.60
Identities = 24/101 (23%), Positives = 48/101 (47%), Gaps = 13/101 (12%)
Frame = +3
Query: 168 ITEKNSNIATMSRVLNENMKSHKTVAD---KSVREVDGLKKSLRE----------ERKMK 308
+ E N I ++ +VL + ++S +++ D + E+D LKK + E E K K
Sbjct: 163 LRESNGPIHSLIKVLTDRIRSLQSIKDILKREEAEIDRLKKEIEEIKVKLENIEREAKEK 222
Query: 309 QSAFTKVDDLISQVYEFEAAFAIVKGSTPVNGVNSKTDSIS 431
+ + + +++ E + + I+ G V VN K + I+
Sbjct: 223 EDELNQYNTEFNRIKEIKVQYDILSGELSV--VNKKIEEIA 261
>sp|Q92614|MY18A_HUMAN Myosin-18A (Myosin XVIIIa) (Myosin containing PDZ domain) (Molecule
associated with JAK3 N-terminus) (MAJN)
Length = 2054
Score = 33.5 bits (75), Expect = 0.78
Identities = 25/89 (28%), Positives = 41/89 (46%), Gaps = 3/89 (3%)
Frame = +3
Query: 93 AERKAATDKQMALVKQMHIDQLMSEITEKNSNIATMSRVLNENMKSHKTV---ADKSVRE 263
A+ K A ++Q++ +L E E + + +NE MK HK A + + +
Sbjct: 1724 AKAKTALEEQLS--------RLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQ 1775
Query: 264 VDGLKKSLREERKMKQSAFTKVDDLISQV 350
++ L+ L E K KQ K+ L SQV
Sbjct: 1776 INDLQAQLEEANKEKQELQEKLQALQSQV 1804
>sp|P36130|YK16_YEAST Hypothetical 74.7 kDa Trp-Asp repeats containing protein in
DAL80-GAP1 intergenic region
Length = 659
Score = 33.1 bits (74), Expect = 1.0
Identities = 35/164 (21%), Positives = 70/164 (42%), Gaps = 10/164 (6%)
Frame = +3
Query: 135 KQMHIDQLMSEITEKNSNIATMSRVLNENMKSHKTVADKSVREVDGLKKSLREERKMKQS 314
+++ I + + I K++N+ ++ + + + ++ RE L+K + EER + S
Sbjct: 215 RKLKILERVKHIDSKSTNLENDVTLIKDRINFIEEYNLEADRE-QSLRKQMEEERSSEAS 273
Query: 315 AFTKVDDLISQVYEFEAAFAIVKG--STPVNGVNSKTDSISCSQISRPST--------GV 464
+FT+ ++ IS + + E+ +K P + K I SR +T G
Sbjct: 274 SFTQNEEAISSLCDVESKDTRLKDFYKMPHEKSHDKNRQIISETYSRNTTAFRMTIPHGE 333
Query: 465 GGNLKSGLKPITPDMKISSTQTRPRPITYGDILRRYPIPYAPDH 596
GN + L TP + S+ + R + D+ + P H
Sbjct: 334 HGNSITALDFDTPWGTLCSSSYQDRIVKVWDLNHGIQVGELPGH 377
>sp|O15083|ERC2_HUMAN ERC protein 2
Length = 957
Score = 32.7 bits (73), Expect = 1.3
Identities = 24/85 (28%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = +3
Query: 87 HAAERKAATDKQMALVKQMHIDQLMSEITEKNSNIATMSRVLNENMKSHKTVADKSVREV 266
H A + KQ+ + +M + L++ I+EK++NIA + ++ K+ + V RE
Sbjct: 836 HLANLRIERRKQLEEILEMKQEALLAAISEKDANIALLELSASKKKKTQEEVMALK-REK 894
Query: 267 DGLKKSLRE--ERKMKQSAFTKVDD 335
D L L++ + +MK A DD
Sbjct: 895 DRLVHQLKQQTQNRMKLMADNYDDD 919
>sp|P47540|P115_MYCGE Protein P115 homolog
Length = 982
Score = 32.7 bits (73), Expect = 1.3
Identities = 27/138 (19%), Positives = 62/138 (44%), Gaps = 9/138 (6%)
Frame = +3
Query: 21 EKELNDL---------RKTLXXXXXXXXXXXHAAERKAATDKQMALVKQMHIDQLMSEIT 173
+KEL D+ RK + ++KAA K++ LV Q +D ++++
Sbjct: 289 QKELQDIYQKINELEQRKVIIDVQLRQGFSQKDEKQKAAALKKLILVDQTQLDGFENQLS 348
Query: 174 EKNSNIATMSRVLNENMKSHKTVADKSVREVDGLKKSLREERKMKQSAFTKVDDLISQVY 353
+ I + +++NE K++ D+ +++ L +R +K T ++ +++
Sbjct: 349 NSKTTITDLEKLINE----QKSLVDQIKLQIEKNTADLIYQRSLK----TIIELQTNELK 400
Query: 354 EFEAAFAIVKGSTPVNGV 407
+ A +VK + + G+
Sbjct: 401 KTNNANILVKNANALTGI 418
>sp|P08928|LAM0_DROME Lamin Dm0
Length = 622
Score = 32.7 bits (73), Expect = 1.3
Identities = 25/93 (26%), Positives = 45/93 (48%), Gaps = 5/93 (5%)
Frame = +3
Query: 93 AERKAATDKQMALVKQMHIDQLMSEITEKNSNIATMSRVLNENMKSHKTVADKSVREVDG 272
A+RK + +K++ + E T KN T+SRV EN +S+RE
Sbjct: 176 ADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLEN-------TIQSLREELS 228
Query: 273 LK-----KSLREERKMKQSAFTKVDDLISQVYE 356
K + + E R++KQ+ ++++D +S Y+
Sbjct: 229 FKDQIHSQEINESRRIKQTEYSEIDGRLSSEYD 261
>sp|Q8K3M6|ERC2_RAT ERC protein 2 (CAZ-associated structural protein 1) (CAST1) (Cast)
(Cytomatrix protein p110)
Length = 957
Score = 32.7 bits (73), Expect = 1.3
Identities = 24/85 (28%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = +3
Query: 87 HAAERKAATDKQMALVKQMHIDQLMSEITEKNSNIATMSRVLNENMKSHKTVADKSVREV 266
H A + KQ+ + +M + L++ I+EK++NIA + ++ K+ + V RE
Sbjct: 836 HLANLRMERRKQLEEILEMKQEALLAAISEKDANIALLELSASKKKKTQEEVMALK-REK 894
Query: 267 DGLKKSLRE--ERKMKQSAFTKVDD 335
D L L++ + +MK A DD
Sbjct: 895 DRLVHQLKQQTQNRMKLMADNYDDD 919
>sp|Q60HE6|MRE11_MACFA Double-strand break repair protein MRE11A (MRE11 homolog 1) (MRE11
meiotic recombination 11 homolog A)
Length = 707
Score = 32.7 bits (73), Expect = 1.3
Identities = 24/81 (29%), Positives = 38/81 (46%)
Frame = +3
Query: 102 KAATDKQMALVKQMHIDQLMSEITEKNSNIATMSRVLNENMKSHKTVADKSVREVDGLKK 281
K +K +K+ HID L +I E+ R E+ + + D VRE +
Sbjct: 475 KYQLEKTQRFLKERHIDALEDKIDEE-------VRRFRESRQKNTNEEDDEVREAMTRAR 527
Query: 282 SLREERKMKQSAFTKVDDLIS 344
+LR + + SAF+ DDL+S
Sbjct: 528 ALRSQSEESASAFS-ADDLMS 547
>sp|O29230|RAD50_ARCFU DNA double-strand break repair rad50 ATPase
Length = 886
Score = 32.7 bits (73), Expect = 1.3
Identities = 22/90 (24%), Positives = 47/90 (52%), Gaps = 3/90 (3%)
Frame = +3
Query: 96 ERKAATDKQMALVKQMHIDQLMSEITEKNSNIATMSRVLNEN---MKSHKTVADKSVREV 266
E+KA ++ +K I+ L +++E+ N+ + + L E+ ++S + ++EV
Sbjct: 191 EKKAEIERISEEIKS--IESLREKLSEEVRNLESRLKELEEHKSRLESLRKQESSVLQEV 248
Query: 267 DGLKKSLREERKMKQSAFTKVDDLISQVYE 356
GL++ LRE K + +++DL + E
Sbjct: 249 RGLEEKLRELEKQLKEVVERIEDLEKKAKE 278
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,996,120
Number of Sequences: 369166
Number of extensions: 1672401
Number of successful extensions: 4735
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4582
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4734
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)