Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_G17
(712 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-... 209 4e-54
sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 ... 209 8e-54
sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-... 209 8e-54
sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-re... 208 1e-53
sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-... 207 2e-53
sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated ... 206 5e-53
sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic ret... 204 2e-52
sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated ... 184 2e-46
sp|Q08277|HSP82_MAIZE Heat shock protein 82 145 1e-34
sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2) 142 7e-34
>sp|P41148|ENPL_CANFA Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 209 bits (533), Expect = 4e-54
Identities = 105/213 (49%), Positives = 143/213 (67%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKFD+S+K KE RE I KEFEPLL W KD AL + IEKA +
Sbjct: 577 IQALPEFDGKRFQNVAKEGVKFDESEKTKESREAIEKEFEPLLNWMKDKALKDKIEKAVV 636
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+GNMERIMK +SQKK EINPRHPLIK+
Sbjct: 637 SQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIKD 696
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R+V+++ D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI +
Sbjct: 697 MLRRVKEDEDDKTVSDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNI------D 750
Query: 543 DDEPIEEKPAEDEGEVPEPPKTEQPIENKEAVD 641
D +EE+P E+ E E + +++E +D
Sbjct: 751 PDAKVEEEPEEEPEETTEDTTEDTEQDDEEEMD 783
>sp|P08110|ENPL_CHICK Endoplasmin precursor (Heat shock 108 kDa protein) (HSP108) (HSP 108)
(Transferrin-binding protein)
Length = 795
Score = 209 bits (531), Expect = 8e-54
Identities = 105/198 (53%), Positives = 139/198 (70%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKF++S+K+KE RE + KEFEPLL W KD AL + IEKA +
Sbjct: 576 IQALPEFDGKRFQNVAKEGVKFEESEKSKESREALEKEFEPLLNWMKDKALKDKIEKAVL 635
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+GNMERIMK +SQKK EINPRHPLIK+
Sbjct: 636 SQRLTQSPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIKD 695
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R+V++N D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE
Sbjct: 696 MLRRVKENEDDKTVSDLAVVLFETATLRSGYMLPDTKEYGDRIERMLRLSLNIDLDAKVE 755
Query: 543 DDEPIEEKPAEDEGEVPE 596
+EP E + A +E E E
Sbjct: 756 -EEPEEPEDAAEEAEQDE 772
>sp|Q95M18|ENPL_BOVIN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
Length = 804
Score = 209 bits (531), Expect = 8e-54
Identities = 104/214 (48%), Positives = 144/214 (67%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKFD+S+K+KE RE + KEFEPLL W KD AL + IEKA +
Sbjct: 577 IQALPEFDGKRFQNVAKEGVKFDESEKSKESREAVEKEFEPLLNWMKDKALKDKIEKAVV 636
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+GNMERIMK +SQKK EINPRHPLI++
Sbjct: 637 SQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRD 696
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R+V+++ D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE
Sbjct: 697 MLRRVKEDEDDKTVSDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVE 756
Query: 543 DDEPIEEKPAEDEGEVPEPPKTEQPIENKEAVDK 644
++ EE+P E + E + ++ E D+
Sbjct: 757 EEP--EEEPEETTEDTAEDTEQDEEEEMDAGTDE 788
>sp|Q29092|ENPL_PIG Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(GP96 homolog) (98 kDa protein kinase) (PPK 98) (ppk98)
Length = 804
Score = 208 bits (529), Expect = 1e-53
Identities = 104/214 (48%), Positives = 143/214 (66%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKFD+S+K+KE RE + KEFEPLL W KD AL + IEKA +
Sbjct: 577 IQALPEFDGKRFQNVAKEGVKFDESEKSKENREAVEKEFEPLLNWMKDKALKDKIEKAVV 636
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+GNMERIMK +SQKK EINPRHPLI++
Sbjct: 637 SQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRD 696
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R+V+++ D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE
Sbjct: 697 MLRRVKEDEDDKTVSDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVE 756
Query: 543 DDEPIEEKPAEDEGEVPEPPKTEQPIENKEAVDK 644
++ EE+P E + E + + E D+
Sbjct: 757 EEP--EEEPEETTEDTTEDTEQDDDEEMDAGADE 788
>sp|P14625|ENPL_HUMAN Endoplasmin precursor (94 kDa glucose-regulated protein) (GRP94)
(gp96 homolog) (Tumor rejection antigen 1)
Length = 803
Score = 207 bits (528), Expect = 2e-53
Identities = 104/205 (50%), Positives = 141/205 (68%), Gaps = 1/205 (0%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKFD+S+K KE RE + KEFEPLL W KD AL + IEKA +
Sbjct: 577 IQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVV 636
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+GNMERIMK +SQKK EINPRHPLI++
Sbjct: 637 SQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRD 696
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R+++++ D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI D VE
Sbjct: 697 MLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVE 756
Query: 543 DD-EPIEEKPAEDEGEVPEPPKTEQ 614
++ E E+ AED E E + E+
Sbjct: 757 EEPEEEPEETAEDTTEDTEQDEDEE 781
>sp|P08712|ENPL_MESAU Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 400
Score = 206 bits (524), Expect = 5e-53
Identities = 102/210 (48%), Positives = 141/210 (67%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKFD+S+K KE RE KEFEPLL W KD AL + IEKA +
Sbjct: 174 IQALPEFDGKRFQNVAKEGVKFDESEKTKENREATEKEFEPLLNWMKDKALKDKIEKAVV 233
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+GNMERIMK +SQKK EINPRHPLI++
Sbjct: 234 SQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRD 293
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R+V+++ D+ D+A+ L++TA LRSGY L + +ADRIE++LR SLNI + VE
Sbjct: 294 MLRRVKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYADRIERMLRLSLNIDPEAQVE 353
Query: 543 DDEPIEEKPAEDEGEVPEPPKTEQPIENKE 632
++ E + ++ E E + + E +E
Sbjct: 354 EEPEEEPEDTTEDTEQDEEEEVDAGTEEEE 383
>sp|P08113|ENPL_MOUSE Endoplasmin precursor (Endoplasmic reticulum protein 99) (94 kDa
glucose-regulated protein) (GRP94) (ERP99) (Polymorphic
tumor rejection antigen 1) (Tumor rejection antigen gp96)
Length = 802
Score = 204 bits (519), Expect = 2e-52
Identities = 106/223 (47%), Positives = 145/223 (65%), Gaps = 9/223 (4%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKFD+S+K KE RE KEFEPLL W KD AL + IEKA +
Sbjct: 577 IQALPEFDGKRFQNVAKEGVKFDESEKTKESREATEKEFEPLLNWMKDKALKDKIEKAVV 636
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+GNMERIMK +SQKK EINPRHPLI++
Sbjct: 637 SQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRD 696
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R+++++ D+ D+A+ L++TA LRSGY L + + DRIE++LR SLNI + VE
Sbjct: 697 MLRRIKEDEDDKTVMDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPEAQVE 756
Query: 543 DDEPIE--------EKPAEDEGEVPEPPKTEQPIE-NKEAVDK 644
++ E E +DEGE + E+ E KE+ +K
Sbjct: 757 EEPEEEPEDTSEDAEDSEQDEGEEMDAGTEEEEEETEKESTEK 799
>sp|O18750|ENPL_RABIT Endoplasmin (94 kDa glucose-regulated protein) (GRP94)
Length = 716
Score = 184 bits (468), Expect = 2e-46
Identities = 102/204 (50%), Positives = 133/204 (65%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+Q++ EF+GK+ QN+ KEGVKFD+S+K KE RE KEFEPLL W KD AL + IEKA +
Sbjct: 496 IQALPEFDGKRFQNVAKEGVKFDESEKTKESREATEKEFEPLLNWMKDKALKDKIEKAVV 555
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+RL SPCA+VAS YGW+ NMERIMK S +K EINPRHPLI++
Sbjct: 556 SQRLTESPCALVASQYGWSANMERIMKAQAYQTGKDSTKYYAS--QKTFEINPRHPLIRD 613
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
+ R E D+ D+A+ L++TA LRSGY L + + DRIE+I+R SLNI D VE
Sbjct: 614 MLRIKE---DDKTVMDLAVVLFETAILRSGYLLPDTKAYGDRIERIVRLSLNIDPDAKVE 670
Query: 543 DDEPIEEKPAEDEGEVPEPPKTEQ 614
++ EE+P ED E E + E+
Sbjct: 671 EEP--EEEP-EDTTEDTEQDEEEE 691
>sp|Q08277|HSP82_MAIZE Heat shock protein 82
Length = 715
Score = 145 bits (366), Expect = 1e-34
Identities = 83/205 (40%), Positives = 120/205 (58%), Gaps = 2/205 (0%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSD--KAKEQREIISKEFEPLLKWFKDVALSEHIEKA 176
V + E++GKKL + KEG+K DD D +AK++RE K FE L K KD+ L + +EK
Sbjct: 516 VGQLKEYDGKKLVSATKEGLKLDDEDDEEAKKRREERKKRFEELCKVIKDI-LGDRVEKV 574
Query: 177 EISERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLI 356
+S+R+ SPC +V YGWT NMERIMK S KK +EINP + ++
Sbjct: 575 VVSDRIVDSPCCLVTGEYGWTANMERIMKAQALRDSSMSAYM---SSKKTMEINPDNGIM 631
Query: 357 KELKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEA 536
+EL+++ E + +D+ +D+ L L++TA L SG+SL FA RI ++L+ LNI D A
Sbjct: 632 EELRKRAEADRNDKSVKDLVLLLFETALLTSGFSLDDPNTFAARIHRMLKLGLNIDEDAA 691
Query: 537 VEDDEPIEEKPAEDEGEVPEPPKTE 611
++D + PA DEG E E
Sbjct: 692 ADED---ADMPALDEGAAEESKMEE 713
>sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2)
Length = 699
Score = 142 bits (359), Expect = 7e-34
Identities = 79/204 (38%), Positives = 122/204 (59%)
Frame = +3
Query: 3 VQSVTEFEGKKLQNLGKEGVKFDDSDKAKEQREIISKEFEPLLKWFKDVALSEHIEKAEI 182
+ + EFEGKKL + KEG+K D+++ K+++E + ++FE L K KDV L + +EK +
Sbjct: 501 IGQLKEFEGKKLVSATKEGLKLDETEDEKKKKEELKEKFEGLCKVIKDV-LGDKVEKVIV 559
Query: 183 SERLHSSPCAVVASTYGWTGNMERIMKXXXXXXXXXXXXXXXSSQKKILEINPRHPLIKE 362
S+R+ SPC +V YGWT NMERIMK S KK +EINP + ++ E
Sbjct: 560 SDRVVDSPCCLVTGEYGWTANMERIMKAQALRDSSMAGYM---SSKKTMEINPENSIMDE 616
Query: 363 LKRQVEKNPSDEQARDIALTLYDTANLRSGYSLQASGGFADRIEKILRTSLNIPHDEAVE 542
L+++ + + +D+ +D+ L L++TA L SG+SL F RI ++L+ L+I D+AVE
Sbjct: 617 LRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIDDDDAVE 676
Query: 543 DDEPIEEKPAEDEGEVPEPPKTEQ 614
D E P ED+ + E K E+
Sbjct: 677 AD--AEMPPLEDDADA-EGSKMEE 697
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,011,799
Number of Sequences: 369166
Number of extensions: 1429705
Number of successful extensions: 6618
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5670
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6396
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6267895215
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)