Planarian EST Database


Dr_sW_025_D07

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_025_D07
         (844 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P0A0C7|pre2_STAAU  Plasmid recombination enzyme (Mobiliza...    36   0.15 
sp|P30319|DPOL_CBEPV  DNA polymerase                               34   0.58 
sp|P62134|RAD50_METMP  DNA double-strand break repair rad50 ...    34   0.58 
sp|P40750|PBPD_BACSU  Penicillin-binding protein 4 precursor...    32   1.7  
sp|Q20060|SMC4_CAEEL  Structural maintenance of chromosome 4...    32   1.7  
sp|P47729|DPO3_MYCPU  DNA polymerase III polC-type (PolIII)        32   2.9  
sp|Q58718|RAD50_METJA  DNA double-strand break repair rad50 ...    32   2.9  
sp|Q00799|RBP2_PLAVB  Reticulocyte binding protein 2 precurs...    31   3.7  
sp|Q9Z1N5|UAP56_MOUSE  Spliceosome RNA helicase Bat1 (DEAD-b...    31   4.9  
sp|Q13838|UAP56_HUMAN  Spliceosome RNA helicase BAT1 (DEAD-b...    31   4.9  
>sp|P0A0C7|pre2_STAAU Plasmid recombination enzyme (Mobilization protein)
 sp|P0A0C6|pre2_STAAN Plasmid recombination enzyme (Mobilization protein)
 sp|P0A0C5|pre2_STAAM Plasmid recombination enzyme (Mobilization protein)
          Length = 420

 Score = 35.8 bits (81), Expect = 0.15
 Identities = 19/54 (35%), Positives = 32/54 (59%)
 Frame = +3

Query: 474 ISQKHREVTNYYDGIIERFIKEQNNMNELVKIFNDWKIKCEKNISELPKDIDKI 635
           +S++H +V   Y G++ERF +  N+ NEL++     K K    IS+L +D+  I
Sbjct: 302 LSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK----ISDLKRDVSLI 351
>sp|P30319|DPOL_CBEPV DNA polymerase
          Length = 964

 Score = 33.9 bits (76), Expect = 0.58
 Identities = 15/60 (25%), Positives = 31/60 (51%)
 Frame = +3

Query: 75   IDNSCIMMTIEQMNRYFRENITLQEIIEQYKNVVMLKFRNFSNEIKSIFQNQHDKNEIYS 254
            I  + +  TI+ +  Y   N T+Q++  +  N +M  F+N    I+++  N+  K+  Y+
Sbjct: 849  IHKTILKNTIDILKEYLTNNCTIQDVNNKINNYLMFTFKNIIENIQNLDINEFKKSVKYT 908
>sp|P62134|RAD50_METMP DNA double-strand break repair rad50 ATPase
          Length = 993

 Score = 33.9 bits (76), Expect = 0.58
 Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 4/75 (5%)
 Frame = +3

Query: 402 NEKKLAENINDELKECL-NSHIEILISQKHREVTNYYD---GIIERFIKEQNNMNELVKI 569
           NEKK  EN+ DELK  + N   EI + ++ + + N      GI+E ++K +   ++    
Sbjct: 652 NEKKSLENLKDELKNTIYNLEREINLKKELKNIQNDISSKIGIVECYVKWETEKSDFENK 711

Query: 570 FNDWKIKCEKNISEL 614
            ++ K   EK +  L
Sbjct: 712 LSECKENYEKYMESL 726
>sp|P40750|PBPD_BACSU Penicillin-binding protein 4 precursor (PBP 4)
          Length = 624

 Score = 32.3 bits (72), Expect = 1.7
 Identities = 20/70 (28%), Positives = 34/70 (48%)
 Frame = +3

Query: 33  KHMNGLDGQLKQIFIDNSCIMMTIEQMNRYFRENITLQEIIEQYKNVVMLKFRNFSNEIK 212
           K M  LD Q+    ID   I + ++    Y R+   + EI+  ++N V++ F     E+K
Sbjct: 32  KQMKSLD-QVLDKNIDLKDISL-VQNSYMYDRDGSLVSEIVSDHENRVLVPFNKIPEEVK 89

Query: 213 SIFQNQHDKN 242
            IF    D++
Sbjct: 90  QIFLTSEDRH 99
>sp|Q20060|SMC4_CAEEL Structural maintenance of chromosome 4 (Protein smc-4)
          Length = 1549

 Score = 32.3 bits (72), Expect = 1.7
 Identities = 20/71 (28%), Positives = 37/71 (52%)
 Frame = +3

Query: 405 EKKLAENINDELKECLNSHIEILISQKHREVTNYYDGIIERFIKEQNNMNELVKIFNDWK 584
           E ++A    ++LK+  NS  + LI  K R      +   E + KE++ +++L   F+ W 
Sbjct: 504 EARIAREDFEDLKKLANSGTDKLIELKKR-----LESSEESYAKEKDELDKLKPEFDSWN 558

Query: 585 IKCEKNISELP 617
            K ++  +ELP
Sbjct: 559 DKLKQLSTELP 569
>sp|P47729|DPO3_MYCPU DNA polymerase III polC-type (PolIII)
          Length = 1435

 Score = 31.6 bits (70), Expect = 2.9
 Identities = 27/87 (31%), Positives = 42/87 (48%), Gaps = 4/87 (4%)
 Frame = +3

Query: 387 IPEDNNEKKLAENINDELKECLNSHIEILISQKHREVTNYYDGIIERFIKEQNNMNELVK 566
           IPE     K+A   +DE K   +  I+ L + K  +  ++Y       +K +N +N+LVK
Sbjct: 16  IPEYLKNTKIANPYHDEEKSFFSCEIDFLETPKFEDFKSFY-------LKTKNFLNQLVK 68

Query: 567 ----IFNDWKIKCEKNISELPKDIDKI 635
               +F    I  EK  SE+ K +D I
Sbjct: 69  EQKLLFKIENIFYEK--SEIKKYLDWI 93
>sp|Q58718|RAD50_METJA DNA double-strand break repair rad50 ATPase
          Length = 1005

 Score = 31.6 bits (70), Expect = 2.9
 Identities = 40/180 (22%), Positives = 75/180 (41%), Gaps = 4/180 (2%)
 Frame = +3

Query: 105 EQMNRYFRENITLQEIIEQYKNVVMLKFRNFSNEIKSIFQNQHDKNEIYSQLRLLFKVAP 284
           E+   Y+ + + L+E   +Y N + L++     E KSI +N    N++ +++  L +   
Sbjct: 388 EECKEYYEKYLELEEKAVEY-NKLTLEYITLLQEKKSIEKNI---NDLETRINKLLEETK 443

Query: 285 PVLLAAGTALMKINPXXXXXXXXXXXXXXXXDSSIPEDNNEKKLAENINDELKE----CL 452
            + + +    +K                   +  + E N+E K  + I DELKE    C 
Sbjct: 444 NIDIESIENSLKEIEEKKKVLENLQKEKIELNKKLGEINSEIKRLKKILDELKEVEGKCP 503

Query: 453 NSHIEILISQKHREVTNYYDGIIERFIKEQNNMNELVKIFNDWKIKCEKNISELPKDIDK 632
                I    K  E+ N +   +     E   +N+ ++       + EK+I +L K+IDK
Sbjct: 504 LCKTPI-DENKKMELINQHKTQLNNKYTELEEINKKIR-------EIEKDIEKLKKEIDK 555
>sp|Q00799|RBP2_PLAVB Reticulocyte binding protein 2 precursor (PvRBP-2)
          Length = 2867

 Score = 31.2 bits (69), Expect = 3.7
 Identities = 22/61 (36%), Positives = 33/61 (54%), Gaps = 2/61 (3%)
 Frame = +3

Query: 84   SCIMMTIEQMNRYFRENITLQEIIEQYKNVVMLKFRNFSNEIKSIFQ--NQHDKNEIYSQ 257
            S IM  +E +NR F EN+  +E + Q +N       N+ NEI SIF   N  D +E  ++
Sbjct: 2471 SDIMKRVEDLNRRFTENLPEKEKLHQIEN-------NY-NEISSIFSEINLQDVDEFVAK 2522

Query: 258  L 260
            +
Sbjct: 2523 I 2523
>sp|Q9Z1N5|UAP56_MOUSE Spliceosome RNA helicase Bat1 (DEAD-box protein UAP56) (56 kDa
           U2AF65 associated protein) (HLA-B associated transcript
           1)
 sp|Q63413|UAP56_RAT Spliceosome RNA helicase Bat1 (DEAD-box protein UAP56) (56 kDa
           U2AF65 associated protein) (ATP-dependent RNA helicase
           p47)
          Length = 428

 Score = 30.8 bits (68), Expect = 4.9
 Identities = 16/39 (41%), Positives = 24/39 (61%)
 Frame = +3

Query: 513 GIIERFIKEQNNMNELVKIFNDWKIKCEKNISELPKDID 629
           G+   F+ ++N+     KI ND + + E NISELP +ID
Sbjct: 385 GLAITFVSDEND----AKILNDVQDRFEVNISELPDEID 419
>sp|Q13838|UAP56_HUMAN Spliceosome RNA helicase BAT1 (DEAD-box protein UAP56) (56 kDa
           U2AF65 associated protein) (ATP-dependent RNA helicase
           p47) (HLA-B associated transcript-1)
 sp|Q29024|UAP56_PIG Spliceosome RNA helicase BAT1 (DEAD-box protein UAP56) (56 kDa
           U2AF65 associated protein)
 sp|P60024|UAP56_PANTR Spliceosome RNA helicase BAT1 (DEAD-box protein UAP56) (56 kDa
           U2AF65 associated protein)
 sp|Q5TM17|UAP56_MACMU Spliceosome RNA helicase BAT1 (DEAD-box protein UAP56) (56 kDa
           U2AF65 associated protein)
 sp|Q5RE47|UAP56_PONPY Spliceosome RNA helicase BAT1 (DEAD-box protein UAP56) (56 kDa
           U2AF65 associated protein)
          Length = 428

 Score = 30.8 bits (68), Expect = 4.9
 Identities = 16/39 (41%), Positives = 24/39 (61%)
 Frame = +3

Query: 513 GIIERFIKEQNNMNELVKIFNDWKIKCEKNISELPKDID 629
           G+   F+ ++N+     KI ND + + E NISELP +ID
Sbjct: 385 GLAITFVSDEND----AKILNDVQDRFEVNISELPDEID 419
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,667,908
Number of Sequences: 369166
Number of extensions: 1358209
Number of successful extensions: 4168
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3983
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4146
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)