Planarian EST Database


Dr_sW_025_C11

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_025_C11
         (436 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P59999|ARPC4_MOUSE  Actin-related protein 2/3 complex sub...   131   5e-31
sp|P58798|ARPC4_CAEEL  Probable actin-related protein 2/3 co...   120   2e-27
sp|P33204|ARPC4_YEAST  ARP2/3 complex 20 kDa subunit (p20-ARC)    107   8e-24
sp|Q92352|ARPC4_SCHPO  Probable ARP2/3 complex 20 kDa subuni...   100   2e-21
sp|Q8XJ55|DAPB_CLOPE  Dihydrodipicolinate reductase (DHPR)         32   0.76 
sp|Q7MK41|PNCB_VIBVY  Nicotinate phosphoribosyltransferase (...    30   2.9  
sp|Q8DA38|PNCB_VIBVU  Nicotinate phosphoribosyltransferase (...    30   2.9  
sp|O94466|RGA7_SCHPO  Probable Rho-GTPase-activating protein 7     29   3.8  
sp|O51363|SYP_BORBU  Prolyl-tRNA synthetase (Proline--tRNA l...    29   3.8  
sp|Q8TDY2|RBCC1_HUMAN  RB1-inducible coiled-coil protein 1         29   5.0  
>sp|P59999|ARPC4_MOUSE Actin-related protein 2/3 complex subunit 4 (ARP2/3 complex 20 kDa
           subunit) (p20-ARC)
 sp|P59998|ARPC4_HUMAN Actin-related protein 2/3 complex subunit 4 (ARP2/3 complex 20 kDa
           subunit) (p20-ARC)
          Length = 168

 Score =  131 bits (330), Expect = 5e-31
 Identities = 76/145 (52%), Positives = 98/145 (67%), Gaps = 12/145 (8%)
 Frame = +1

Query: 25  PYLAAVRHSLEAALCLSNFDSQVVERHNKPEVETKSSKELLLTPIVISRNDKEKVLIEGS 204
           PYL+AVR +L+AALCL NF SQVVERHNKPEVE +SSKELLL P+ ISRN+KEKVLIEGS
Sbjct: 7   PYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGS 66

Query: 205 INSVRLSISITQSDELRRCFVIIY-RFMMMRAEES*LRRNSQIRYSSIL-----CHTDN- 363
           INSVR+SI++ Q+DE+ +     + RFMMMRAE   + R   +    I       HT+  
Sbjct: 67  INSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITNFHTEQM 126

Query: 364 -----VSTIVDYIDMRYDRISEMRL 423
                V  ++ +++     ISEM+L
Sbjct: 127 YKHKLVDFVIHFMEEIDKEISEMKL 151
>sp|P58798|ARPC4_CAEEL Probable actin-related protein 2/3 complex subunit 4 (ARP2/3
           complex 20 kDa subunit) (p20-ARC)
          Length = 169

 Score =  120 bits (300), Expect = 2e-27
 Identities = 59/94 (62%), Positives = 77/94 (81%), Gaps = 1/94 (1%)
 Frame = +1

Query: 22  EPYLAAVRHSLEAALCLSNFDSQVVERHNKPEVETKSSKELLLTPIVISRNDKEKVLIEG 201
           +PYL AVRH+L+AALCL  F SQVVERHNKPEVE ++SKELL+TP+V++RN +E+VLIE 
Sbjct: 6   QPYLEAVRHTLQAALCLEQFSSQVVERHNKPEVEVQTSKELLMTPVVVARNKQERVLIEP 65

Query: 202 SINSVRLSISITQSDELRRCFVIIY-RFMMMRAE 300
           S+NSVR+SI+I QSDE+ +     + RFM  RA+
Sbjct: 66  SVNSVRISIAIKQSDEIEKILCHKFTRFMCQRAD 99
>sp|P33204|ARPC4_YEAST ARP2/3 complex 20 kDa subunit (p20-ARC)
          Length = 171

 Score =  107 bits (268), Expect = 8e-24
 Identities = 69/146 (47%), Positives = 95/146 (65%), Gaps = 13/146 (8%)
 Frame = +1

Query: 25  PYLAAVRHSLEAALCLSNFDSQVVERHNKPEVET-KSSKELLLTPIVISRNDKEKVLIEG 201
           PYL AVR+SLEAAL LSNF SQ VERHN+PEVE   +S ELLL P+ ISRN+ E+VLIE 
Sbjct: 7   PYLTAVRYSLEAALTLSNFSSQEVERHNRPEVEVPNTSAELLLQPMHISRNENEQVLIEP 66

Query: 202 SINSVRLSISITQSDELRRCFVIIY-RFMMMRAEES*LRRNSQIRYSSILC-----HTDN 363
           S+NSVR+S+ + Q+DE+ +  V  + RF+  RAE   + R   I   SI       HT++
Sbjct: 67  SVNSVRMSLMVKQADEIEQILVHKFTRFLEQRAEAFYILRRVPIPGYSISFLITNKHTES 126

Query: 364 VST------IVDYIDMRYDRISEMRL 423
           + T      I+++++     ISE++L
Sbjct: 127 MKTGKLVDFIIEFMEDVDKEISEIKL 152
>sp|Q92352|ARPC4_SCHPO Probable ARP2/3 complex 20 kDa subunit (p20-ARC)
          Length = 168

 Score = 99.8 bits (247), Expect = 2e-21
 Identities = 62/149 (41%), Positives = 89/149 (59%), Gaps = 12/149 (8%)
 Frame = +1

Query: 13  NGFEPYLAAVRHSLEAALCLSNFDSQVVERHNKPEVETKSSKELLLTPIVISRNDKEKVL 192
           N   PYL AVR +L A+L L  F S++VER ++PEVE   S E+LL P+V+SRN++E+ L
Sbjct: 3   NTLRPYLNAVRSTLTASLALEEFSSEIVERQSQPEVEVGRSPEILLKPLVVSRNEQEQCL 62

Query: 193 IEGSINSVRLSISITQSDELRRCFVIIY-RFMMMRAEES*LRRNSQIRYSSIL-----CH 354
           IE S+NSVR SI I Q DE+ R  V  + +F+M RAE   + R   ++   I       H
Sbjct: 63  IESSVNSVRFSIRIKQVDEIERILVRKFMQFLMGRAESFFILRRKPVQGYDISFLITNYH 122

Query: 355 TDN------VSTIVDYIDMRYDRISEMRL 423
           T+       V  I+++++     ISEM+L
Sbjct: 123 TEEMLKHKLVDFIIEFMEEVDAEISEMKL 151
>sp|Q8XJ55|DAPB_CLOPE Dihydrodipicolinate reductase (DHPR)
          Length = 254

 Score = 31.6 bits (70), Expect = 0.76
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +1

Query: 58  AALCLSNFDSQVVERHNKPEVETKSSKELLLTPIVISRNDKEKVLIEG 201
           A +   NFD ++VERH+  +V+  S   LLL   +    ++E  L+ G
Sbjct: 128 APVLYENFDIELVERHHNQKVDAPSGTALLLAHTIQDSLNEETKLLYG 175
>sp|Q7MK41|PNCB_VIBVY Nicotinate phosphoribosyltransferase (NAPRTase)
          Length = 437

 Score = 29.6 bits (65), Expect = 2.9
 Identities = 15/50 (30%), Positives = 29/50 (58%)
 Frame = +1

Query: 271 IYRFMMMRAEES*LRRNSQIRYSSILCHTDNVSTIVDYIDMRYDRISEMR 420
           +Y+  MM+A        +Q+RY  I+   DN+S +V+ +    +R++E+R
Sbjct: 19  VYKINMMQAAYR-FYPQTQVRYELIVRSDDNLSDLVEEVREEINRLAELR 67
>sp|Q8DA38|PNCB_VIBVU Nicotinate phosphoribosyltransferase (NAPRTase)
          Length = 437

 Score = 29.6 bits (65), Expect = 2.9
 Identities = 15/50 (30%), Positives = 29/50 (58%)
 Frame = +1

Query: 271 IYRFMMMRAEES*LRRNSQIRYSSILCHTDNVSTIVDYIDMRYDRISEMR 420
           +Y+  MM+A        +Q+RY  I+   DN+S +V+ +    +R++E+R
Sbjct: 19  VYKINMMQAAYR-FYPQTQVRYELIVRSDDNLSDLVEEVREEINRLAELR 67
>sp|O94466|RGA7_SCHPO Probable Rho-GTPase-activating protein 7
          Length = 695

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 16/45 (35%), Positives = 21/45 (46%)
 Frame = +1

Query: 7   LLNGFEPYLAAVRHSLEAALCLSNFDSQVVERHNKPEVETKSSKE 141
           ++   E +     HSL     LS F  QV+E H   E + KS KE
Sbjct: 105 VITSMEIHEQLANHSLTLQKTLSAFSDQVIEFHKNAERKRKSIKE 149
>sp|O51363|SYP_BORBU Prolyl-tRNA synthetase (Proline--tRNA ligase) (ProRS)
          Length = 488

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 19/85 (22%), Positives = 43/85 (50%), Gaps = 5/85 (5%)
 Frame = +1

Query: 139 ELLLTPIVISRNDKEK-----VLIEGSINSVRLSISITQSDELRRCFVIIYRFMMMRAEE 303
           ++LL  + ISR DK++     + ++  I+ V++ + + Q D  +R       F ++  +E
Sbjct: 354 DVLLNSVTISRRDKDRKFKYQISLDSLISKVKVELDLMQKDLFQRAL----NFRILNTKE 409

Query: 304 S*LRRNSQIRYSSILCHTDNVSTIV 378
             + R+S+  Y +   + ++ S  V
Sbjct: 410 --IFRSSKDSYETFKAYVNDYSGFV 432
>sp|Q8TDY2|RBCC1_HUMAN RB1-inducible coiled-coil protein 1
          Length = 1594

 Score = 28.9 bits (63), Expect = 5.0
 Identities = 20/73 (27%), Positives = 34/73 (46%), Gaps = 9/73 (12%)
 Frame = +1

Query: 34   AAVRHSLEAALCLSNFDSQ-------VVERHNKPEVETKSSKELLLTPI--VISRNDKEK 186
            A V+H  EA +CL N   Q       ++   N    E K S+E++L  +  +   ND++ 
Sbjct: 914  ALVKHEKEAVICLQNEKDQKLLEMENIMHSQNCEIKELKQSREIVLEDLKKLHVENDEKL 973

Query: 187  VLIEGSINSVRLS 225
             L+   + S+  S
Sbjct: 974  QLLRAELQSLEQS 986
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,524,869
Number of Sequences: 369166
Number of extensions: 575473
Number of successful extensions: 1400
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1398
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2194785120
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)