Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_B15
(543 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q27655|ENO_FASHE Enolase (2-phosphoglycerate dehydratase... 289 3e-78
sp|P33676|ENO_SCHJA Enolase (2-phosphoglycerate dehydratase... 288 8e-78
sp|O02654|ENO_LOLPE Enolase (2-phosphoglycerate dehydratase... 280 2e-75
sp|P09104|ENOG_HUMAN Gamma-enolase (2-phospho-D-glycerate h... 274 9e-74
sp|P17183|ENOG_MOUSE Gamma-enolase (2-phospho-D-glycerate h... 273 2e-73
sp|Q27527|ENO_CAEEL Enolase (2-phosphoglycerate dehydratase... 273 3e-73
sp|Q27877|ENO_SCHMA Enolase (2-phosphoglycerate dehydratase... 272 3e-73
sp|P07323|ENOG_RAT Gamma-enolase (2-phospho-D-glycerate hyd... 272 4e-73
sp|Q9PVK2|ENOA_ALLMI Alpha-enolase (2-phospho-D-glycerate h... 270 2e-72
sp|P42897|ENO_ALLMI Enolase (2-phosphoglycerate dehydratase... 270 2e-72
>sp|Q27655|ENO_FASHE Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase)
Length = 431
Score = 289 bits (740), Expect = 3e-78
Identities = 140/180 (77%), Positives = 152/180 (84%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAP+I DN EGLELL+ AI+ AGYTGK+ I MD AASEFYKEGKYDLDFKNP S
Sbjct: 206 VGDEGGFAPSIQDNLEGLELLRTAIDKAGYTGKVXIAMDCAASEFYKEGKYDLDFKNPKS 265
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
S W+TS +A VY M+ YPIVSIEDPFDQDDW AWT +G+ IQIVGDDLTVTNP
Sbjct: 266 QASSWITSDAMADVYKKMMSTYPIVSIEDPFDQDDWPAWTKLTGECKIQIVGDDLTVTNP 325
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
RVQK ID KACNCLLLKVNQIGSV+ESI+ACKM+Q AGWGVMVSHRSGETEDN IADLV
Sbjct: 326 LRVQKAIDQKACNCLLLKVNQIGSVSESIKACKMAQEAGWGVMVSHRSGETEDNFIADLV 385
>sp|P33676|ENO_SCHJA Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase)
Length = 434
Score = 288 bits (736), Expect = 8e-78
Identities = 136/180 (75%), Positives = 154/180 (85%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNI DN +GL+LL+ AI+ AGYTGK++IGMD AASE+YK+GKYDLDFKNP S
Sbjct: 207 VGDEGGFAPNIQDNMKGLQLLEEAIKIAGYTGKVEIGMDCAASEYYKKGKYDLDFKNPQS 266
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
+S WL+ E+A VY +MI KYPIVSIEDPFDQDDW AW + IQIVGDDLTVTNP
Sbjct: 267 AESHWLSPDEMANVYKEMIQKYPIVSIEDPFDQDDWDAWPKLTASTNIQIVGDDLTVTNP 326
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R++K I VKACNCLLLKVNQIGS+TESI+ACKM+Q AGWGVMVSHRSGETEDN IADLV
Sbjct: 327 KRIEKAIKVKACNCLLLKVNQIGSITESIEACKMAQKAGWGVMVSHRSGETEDNFIADLV 386
>sp|O02654|ENO_LOLPE Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase)
Length = 434
Score = 280 bits (715), Expect = 2e-75
Identities = 140/180 (77%), Positives = 151/180 (83%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNILDN EGLELLK AI AGYT +I+IGMDVAASEF KE KYDLDFKNP S
Sbjct: 207 VGDEGGFAPNILDNKEGLELLKTAIANAGYTAEIEIGMDVAASEFCKEKKYDLDFKNPDS 266
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
+ + WLTS +LA VY D + YP+VSIEDPFDQDDW A+T + IQIVGDDL VTNP
Sbjct: 267 NPNDWLTSDQLADVYKDFVKNYPVVSIEDPFDQDDWEAYTKMTKDMDIQIVGDDLLVTNP 326
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
RVQKGID+KA N LLLKVNQIGSVTESIQACKMSQ+AGWGVMVSHRSGETED IADLV
Sbjct: 327 KRVQKGIDLKAANALLLKVNQIGSVTESIQACKMSQDAGWGVMVSHRSGETEDTFIADLV 386
>sp|P09104|ENOG_HUMAN Gamma-enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase)
(Neuron-specific enolase) (NSE) (Enolase 2)
Length = 434
Score = 274 bits (701), Expect = 9e-74
Identities = 129/180 (71%), Positives = 154/180 (85%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNIL+N E LEL+K AI+ AGYT KI IGMDVAASEFY++GKYDLDFK+P +
Sbjct: 207 VGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSP-T 265
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
D S+++T +L A+Y D + YP+VSIEDPFDQDDW+AW+ F+ GIQIVGDDLTVTNP
Sbjct: 266 DPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNP 325
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R+++ ++ KACNCLLLKVNQIGSVTE+IQACK++Q GWGVMVSHRSGETED IADLV
Sbjct: 326 KRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLV 385
>sp|P17183|ENOG_MOUSE Gamma-enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase)
(Neuron-specific enolase) (NSE) (Enolase 2)
Length = 434
Score = 273 bits (699), Expect = 2e-73
Identities = 128/180 (71%), Positives = 154/180 (85%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNIL+N E LEL+K AI+ AGYT K+ IGMDVAASEFY++GKYDLDFK+P +
Sbjct: 207 VGDEGGFAPNILENSEALELVKEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSP-A 265
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
D S+++T +L A+Y D + YP+VSIEDPFDQDDW+AW+ F+ GIQIVGDDLTVTNP
Sbjct: 266 DPSRYITGDQLGALYQDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNP 325
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R+++ ++ KACNCLLLKVNQIGSVTE+IQACK++Q GWGVMVSHRSGETED IADLV
Sbjct: 326 KRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLV 385
>sp|Q27527|ENO_CAEEL Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase)
Length = 434
Score = 273 bits (697), Expect = 3e-73
Identities = 131/180 (72%), Positives = 146/180 (81%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNI DN EGL+LL AI+ AGYTGKI IGMDVAASEF+K+GKYDLDFKNP S
Sbjct: 207 VGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISIGMDVAASEFFKDGKYDLDFKNPAS 266
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
D SKWL+ +L +Y I +YP+VSIED FDQDDW W F G IQ+VGDDLTVTNP
Sbjct: 267 DSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDDWDNWGKFHGATSIQLVGDDLTVTNP 326
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R+Q ID K+CNCLLLKVNQIGSVTESI+A K+S+ GWGVMVSHRSGETED IADLV
Sbjct: 327 KRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLSRANGWGVMVSHRSGETEDTFIADLV 386
>sp|Q27877|ENO_SCHMA Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase)
Length = 434
Score = 272 bits (696), Expect = 3e-73
Identities = 128/180 (71%), Positives = 150/180 (83%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNI DN +GL+LL+ AI+ AGYTGK++IGMD AASEF+K GKYDLDFKNPHS
Sbjct: 207 VGDEGGFAPNIQDNMKGLQLLEEAIKIAGYTGKVEIGMDCAASEFHKNGKYDLDFKNPHS 266
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
+S WL+ +A +Y MI K+PIVSIEDPFDQDDW W + IQIVGDDLTVTNP
Sbjct: 267 AESTWLSPDAMANMYKQMISKFPIVSIEDPFDQDDWETWPKLTSSTNIQIVGDDLTVTNP 326
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R+++ I KACNCLLLKVNQIGS+TESI+ACK++Q++GWGVMVSHRSGETED IADLV
Sbjct: 327 KRIKQAIASKACNCLLLKVNQIGSLTESIEACKLAQDSGWGVMVSHRSGETEDTFIADLV 386
>sp|P07323|ENOG_RAT Gamma-enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase)
(Neuron-specific enolase) (NSE) (Enolase 2)
Length = 434
Score = 272 bits (695), Expect = 4e-73
Identities = 128/180 (71%), Positives = 153/180 (85%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNIL+N E LEL+K AI+ AGYT K+ IGMDVAASEFY++GKYDLDFK+P +
Sbjct: 207 VGDEGGFAPNILENSEALELVKEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSP-A 265
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
D S+ +T +L A+Y D + YP+VSIEDPFDQDDW+AW+ F+ GIQIVGDDLTVTNP
Sbjct: 266 DPSRCITGDQLGALYQDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNP 325
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R+++ ++ KACNCLLLKVNQIGSVTE+IQACK++Q GWGVMVSHRSGETED IADLV
Sbjct: 326 KRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLV 385
>sp|Q9PVK2|ENOA_ALLMI Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (Phosphopyruvate
hydratase)
Length = 434
Score = 270 bits (689), Expect = 2e-72
Identities = 129/180 (71%), Positives = 150/180 (83%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNIL+N E LELLK AI AGY+ KI IGMDVAASEFY++GKYDLDFK+P
Sbjct: 207 VGDEGGFAPNILENKEALELLKNAINKAGYSDKIVIGMDVAASEFYRDGKYDLDFKSP-D 265
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
D S+++T +LA +Y + YP+VSIEDPFDQDDW+AW F+ GIQ+VGDDLTVTNP
Sbjct: 266 DPSRYITPDQLADLYKSFVKNYPVVSIEDPFDQDDWAAWKKFTASVGIQVVGDDLTVTNP 325
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R+ K +D KACNCLLLKVNQIGSVTES+QACK++Q+ GWGVMVSHRSGETED IADLV
Sbjct: 326 KRIAKAVDDKACNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLV 385
>sp|P42897|ENO_ALLMI Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase)
Length = 395
Score = 270 bits (689), Expect = 2e-72
Identities = 129/180 (71%), Positives = 150/180 (83%)
Frame = +3
Query: 3 VGDEGGFAPNILDNFEGLELLKVAIEAAGYTGKIKIGMDVAASEFYKEGKYDLDFKNPHS 182
VGDEGGFAPNIL+N E LELLK AI AGY+ KI IGMDVAASEFY++GKYDLDFK+P
Sbjct: 185 VGDEGGFAPNILENKEALELLKNAINKAGYSDKIVIGMDVAASEFYRDGKYDLDFKSP-D 243
Query: 183 DKSKWLTSSELAAVYTDMIHKYPIVSIEDPFDQDDWSAWTDFSGKAGIQIVGDDLTVTNP 362
D S+++T +LA +Y + YP+VSIEDPFDQDDW+AW F+ GIQ+VGDDLTVTNP
Sbjct: 244 DPSRYITPDQLADLYKSFVKNYPVVSIEDPFDQDDWAAWKKFTASVGIQVVGDDLTVTNP 303
Query: 363 TRVQKGIDVKACNCLLLKVNQIGSVTESIQACKMSQNAGWGVMVSHRSGETEDNTIADLV 542
R+ K +D KACNCLLLKVNQIGSVTES+QACK++Q+ GWGVMVSHRSGETED IADLV
Sbjct: 304 KRIAKAVDDKACNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLV 363
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,989,263
Number of Sequences: 369166
Number of extensions: 1256681
Number of successful extensions: 3850
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3247
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3289
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3734833040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)