Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_B03
(579 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 216 2e-56
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 213 4e-55
sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cy... 212 5e-55
sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cy... 210 2e-54
sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cy... 209 4e-54
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 206 3e-53
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 203 3e-52
sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mi... 198 9e-51
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 197 1e-50
sp|P21642|PPCKM_CHICK Phosphoenolpyruvate carboxykinase, mi... 181 9e-46
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 216 bits (551), Expect = 2e-56
Identities = 104/174 (59%), Positives = 131/174 (75%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
V+ L VR ++ E +C PD + +CDGSE+ENK+LI+ L++ G++ L KY+NCWL R
Sbjct: 45 VDLLTTGVRAFVEEGIALCQPDQVHICDGSEQENKVLIKSLLEAGTIVPLPKYDNCWLAR 104
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
T+PADVARVES+TFI T+ + TIP T ++G LGNW+S SDM+AA+ RFPGCMKGR
Sbjct: 105 TNPADVARVESRTFICTERREETIP-TPVEGVKGTLGNWISPSDMDAAVQQRFPGCMKGR 163
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTDSTPFV 522
TMYV+PFSMGP+G PLSKIGIELTDS YVV SM+IMTRMG VL + FV
Sbjct: 164 TMYVVPFSMGPVGSPLSKIGIELTDSAYVVASMRIMTRMGAAVLRQLAKKEEFV 217
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 213 bits (541), Expect = 4e-55
Identities = 103/169 (60%), Positives = 127/169 (75%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
+++LP+ VR+++ A +C PDHI +CDGSEEEN L+ + ++G ++ L KY+NCWL
Sbjct: 20 LDSLPQAVREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLAL 79
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
TDP DVAR+ESKT I T+ +R T+P Q LG WMSE D E A N RFPGCMKGR
Sbjct: 80 TDPRDVARIESKTVIVTQEQRDTVPIPKTGLSQ--LGRWMSEEDFEKAFNARFPGCMKGR 137
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
TMYVIPFSMGP+G PLSKIGIELTDSPYVV SM+IMTRMG VLE++ D
Sbjct: 138 TMYVIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTRMGTPVLEALGD 186
>sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 212 bits (540), Expect = 5e-55
Identities = 102/169 (60%), Positives = 127/169 (75%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
+++LP+ VR ++ A +C PD + +CDGSEEEN+ L+ + ++G +K L KY+NCWL
Sbjct: 20 LDSLPQAVRSFVESSAKLCRPDQVHICDGSEEENRQLLSHMEEEGVIKRLKKYDNCWLAL 79
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
TDP DVAR+ESKT I T+ +R T+P + Q LG WMSE D E A N RFPGCMKGR
Sbjct: 80 TDPRDVARIESKTVIITREQRDTVPIPKNGLSQ--LGRWMSEEDFEKAFNIRFPGCMKGR 137
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
TMYVIPFSMGP+G PLSKIGIELTDSPYVV SM+IMTRMG VLE++ D
Sbjct: 138 TMYVIPFSMGPLGSPLSKIGIELTDSPYVVTSMRIMTRMGTSVLEALGD 186
>sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 210 bits (535), Expect = 2e-54
Identities = 103/169 (60%), Positives = 126/169 (74%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
+++LP+ VR ++ A +C PDHI +CDGSEEEN L+ + ++G ++ L KY+NCWL
Sbjct: 20 LDSLPQAVRKFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLAL 79
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
TDP DVAR+ SKT I T+ +R T+P Q LG WMSE D E ALN RFPGCMKGR
Sbjct: 80 TDPRDVARIGSKTVIVTQEQRDTVPIPKTGLSQ--LGRWMSEEDFEKALNARFPGCMKGR 137
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
TMYVIPFSMGP+G PLSKIGIELTDSPYVV SM+IMTRMG VLE++ D
Sbjct: 138 TMYVIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTRMGTPVLEALGD 186
>sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 209 bits (532), Expect = 4e-54
Identities = 101/167 (60%), Positives = 126/167 (75%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
+ +LP VR++I A +C P+ I +CDGSEEENK +++ +V+QG +K L+KYENCWL
Sbjct: 20 LESLPPQVREFIESNAKLCQPESIHICDGSEEENKKILDIMVEQGMIKKLSKYENCWLAL 79
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
T+P DVAR+ESKT I T+ +R TIP Q LG WMSE D E A N RFPGCM+GR
Sbjct: 80 TNPRDVARIESKTVIITQEQRDTIPIPKTGSSQ--LGRWMSEEDFEKAFNTRFPGCMQGR 137
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESM 501
TMYVIPFSMGPIG PL+KIGIELTDSPYVV SM++MTRMG L+++
Sbjct: 138 TMYVIPFSMGPIGSPLAKIGIELTDSPYVVASMRMMTRMGTAALKAL 184
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 206 bits (525), Expect = 3e-53
Identities = 99/169 (58%), Positives = 126/169 (74%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
+++LP+ VR ++ A +C P++I +CDGSEEE L+ + ++G ++ L KY+NCWL
Sbjct: 20 LDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLAL 79
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
TDP DVAR+ESKT I T+ +R T+P Q +LG WMSE D E A N RFPGCMKGR
Sbjct: 80 TDPRDVARIESKTVIITQEQRDTVPIPKSG--QSQLGRWMSEEDFEKAFNARFPGCMKGR 137
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
TMYVIPFSMGP+G PL+KIGIELTDSPYVV SM+IMTRMG VLE++ D
Sbjct: 138 TMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGD 186
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 203 bits (516), Expect = 3e-52
Identities = 99/169 (58%), Positives = 125/169 (73%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
+++LP+ VR ++ A +C P++I +CDGSEEE L+ + ++G ++ L KY+NCWL
Sbjct: 20 LDSLPQAVRKFVEGNAQLCQPEYIHICDGSEEEYGQLLAHMQEEGVIRKLKKYDNCWLAL 79
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
TDP DVAR+ESKT I T+ +R T+P Q LG WMSE D E A N RFPGCMKGR
Sbjct: 80 TDPRDVARIESKTVIITQEQRDTVPIPKTGLSQ--LGRWMSEEDFEKAFNARFPGCMKGR 137
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
TMYVIPFSMGP+G PL+KIGIELTDSPYVV SM+IMTRMG VLE++ D
Sbjct: 138 TMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGISVLEALGD 186
>sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 198 bits (503), Expect = 9e-51
Identities = 92/166 (55%), Positives = 123/166 (74%)
Frame = +1
Query: 10 LPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVRTDP 189
LP +RD++ A +C P+ I +CDG+E EN + L +QG ++ L KY NCWL RTDP
Sbjct: 40 LPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDP 99
Query: 190 ADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGRTMY 369
DVARVESKT I T +R T+P +G+LGNWMS +D + A+++RFPGCM+GRTMY
Sbjct: 100 KDVARVESKTVIVTPSQRDTVP-LPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMY 158
Query: 370 VIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
V+PFSMGP+G PLS+IG++LTDS YVV SM+IMTR+G VL+++ D
Sbjct: 159 VLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGD 204
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 197 bits (502), Expect = 1e-50
Identities = 91/169 (53%), Positives = 127/169 (75%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
++ LP VRD++ A++C P+ I +CDG+E EN ++ L +QG ++ L KY+NCWL R
Sbjct: 37 MSQLPAGVRDFVARSAHLCQPEGIHICDGTEAENTAILALLEEQGLIRKLPKYKNCWLAR 96
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
TDP DVARVESKT I T +R T+P + +G+LGNWMS + + A+++RFPGCM+GR
Sbjct: 97 TDPKDVARVESKTVIVTPSQRDTVPLLAGGA-RGQLGNWMSPDEFQRAVDERFPGCMQGR 155
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
MYV+PFSMGP+G PLS+IG++LTDS YVV SM+IMTR+G VL+++ D
Sbjct: 156 IMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGD 204
>sp|P21642|PPCKM_CHICK Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 181 bits (460), Expect = 9e-46
Identities = 87/169 (51%), Positives = 115/169 (68%)
Frame = +1
Query: 1 VNTLPKNVRDYIIEKANICIPDHIKVCDGSEEENKILIEDLVKQGSMKSLTKYENCWLVR 180
++ LP RD++ E +C P + +CDGSEEE K L+ L G + L KY+NCWL R
Sbjct: 38 LSALPAAARDFVEEAVRLCRpreVLLCDGSEEEGKELLRGLQDDGVLHPLPKYDNCWLAR 97
Query: 181 TDPADVARVESKTFISTKLKRTTIPETSDPKIQGKLGNWMSESDMEAALNDRFPGCMKGR 360
TDP DVARVESKT + T + +P P +LGNWMS + +AA+ +RFPGCM GR
Sbjct: 98 TDPRDVARVESKTVLVTPEQSDAVPPPP-PSGSPQLGNWMSPNAFQAAVQERFPGCMAGR 156
Query: 361 TMYVIPFSMGPIGGPLSKIGIELTDSPYVVCSMKIMTRMGHKVLESMTD 507
+YVIPFSMGP PL+K+G+++TDSPYVV SM+IMTR+G VL+ + D
Sbjct: 157 PLYVIPFSMGPPTSPLAKLGVQVTDSPYVVLSMRIMTRVGPAVLQRLDD 205
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,503,680
Number of Sequences: 369166
Number of extensions: 1300745
Number of successful extensions: 3661
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3503
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3589
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4259329035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)