Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_A20
(766 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P53990|K0174_HUMAN Protein KIAA0174 (Putative MAPK-activ... 129 7e-30
sp|P53843|YN05_YEAST Hypothetical 34.5 kDa protein in PIK1-... 45 2e-04
sp|Q12446|LAS17_YEAST Proline-rich protein LAS17 41 0.004
sp|Q91YD9|WASL_MOUSE Neural Wiskott-Aldrich syndrome protei... 37 0.059
sp|O95466|FMNL_HUMAN Formin-like 1 protein (Formin-like pro... 36 0.13
sp|Q5R789|ZCHC8_PONPY Zinc finger CCHC domain containing pr... 35 0.22
sp|Q9JL26|FMNL_MOUSE Formin-like 1 protein (Formin-related ... 35 0.22
sp|Q8IN94|OSA_DROME Trithorax group protein osa (Eyelid pro... 35 0.29
sp|O43313|Y0431_HUMAN Hypothetical protein KIAA0431 34 0.38
sp|Q95107|WASL_BOVIN Neural Wiskott-Aldrich syndrome protei... 34 0.50
>sp|P53990|K0174_HUMAN Protein KIAA0174 (Putative MAPK-activating protein PM28)
Length = 364
Score = 129 bits (325), Expect = 7e-30
Identities = 92/272 (33%), Positives = 132/272 (48%), Gaps = 42/272 (15%)
Frame = +2
Query: 2 ATLIWVTPRLQTDVQELKLITDQLHLKYGREYGLACQSNSLNNVNEKVMEKLSIKAPPKR 181
+TLIW PRLQ++V ELK++ DQL KY +EYG C++N + VN+++M KLS++APPK
Sbjct: 101 STLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKI 160
Query: 182 LVEQYLVEIAKSFDVPYEPEEDIDPPASNWNPGEFYAPLPPNLIDF-MGEKPPLINPNAY 358
LVE+YL+EIAK+++VPYEP+ + A PG + +LID + P
Sbjct: 161 LVERYLIEIAKNYNVPYEPDSVVMAEAP---PG-----VETDLIDVGFTDDVKKGGPGRG 212
Query: 359 XXXXXXXXXXXXXXXXXXXXXXXXXISNAPYPYP-QKKPLNDNEAPM------------- 496
+N P+ YP K P + N PM
Sbjct: 213 GSGGFTAPVGGPDGTVPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQ 272
Query: 497 --SLPPKYEEFD----------------GSSSQESA--PPLPPNGPNDLFLPEIPSDQNN 616
+ PP YE D G + SA P P + ++ LPE+PS +
Sbjct: 273 IPATPPSYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPS-VPD 331
Query: 617 TNPPPGSGS-------VDFDDLNRRFEELKRR 691
T P +G+ +DFDDL+RRFEELK++
Sbjct: 332 TLPTASAGASTSASEDIDFDDLSRRFEELKKK 363
>sp|P53843|YN05_YEAST Hypothetical 34.5 kDa protein in PIK1-POL2 intergenic region
Length = 298
Score = 45.1 bits (105), Expect = 2e-04
Identities = 34/105 (32%), Positives = 56/105 (53%)
Frame = +2
Query: 38 DVQELKLITDQLHLKYGREYGLACQSNSLNNVNEKVMEKLSIKAPPKRLVEQYLVEIAKS 217
+V+EL + D + K E+ ++ ++ V EK+++K S P + LV+ YL EIAK+
Sbjct: 121 EVKELSQLKDLMAWKINVEFVNGVIADHID-VPEKIIKKCSPSVPKEELVDLYLKEIAKT 179
Query: 218 FDVPYEPEEDIDPPASNWNPGEFYAPLPPNLIDFMGEKPPLINPN 352
+DVPY E+ +S+ +F P ++ D EKP L N
Sbjct: 180 YDVPYSKLENSLSSSSSNISSDFSDP-SGDIEDNDEEKPILALDN 223
>sp|Q12446|LAS17_YEAST Proline-rich protein LAS17
Length = 633
Score = 40.8 bits (94), Expect = 0.004
Identities = 23/61 (37%), Positives = 32/61 (52%)
Frame = +2
Query: 449 YPYPQKKPLNDNEAPMSLPPKYEEFDGSSSQESAPPLPPNGPNDLFLPEIPSDQNNTNPP 628
+P PL + AP+ PP++ S Q +AP P + P +PEIPS Q+ TNP
Sbjct: 234 HPKHSLPPLPNQFAPLPDPPQHN----SPPQNNAPSQPQSNPFPFPIPEIPSTQSATNPF 289
Query: 629 P 631
P
Sbjct: 290 P 290
>sp|Q91YD9|WASL_MOUSE Neural Wiskott-Aldrich syndrome protein (N-WASP)
Length = 501
Score = 37.0 bits (84), Expect = 0.059
Identities = 23/78 (29%), Positives = 30/78 (38%)
Frame = +2
Query: 455 YPQKKPLNDNEAPMSLPPKYEEFDGSSSQESAPPLPPNGPNDLFLPEIPSDQNNTNPPPG 634
YP P + AP PP S+ PP PP P P +PSD ++ P P
Sbjct: 340 YPPPPPALPSSAPSGPPPPPPPSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPAPS 399
Query: 635 SGSVDFDDLNRRFEELKR 688
D R +LK+
Sbjct: 400 GNKAALLDQIREGAQLKK 417
>sp|O95466|FMNL_HUMAN Formin-like 1 protein (Formin-like protein) (Leukocyte formin)
(CLL-associated antigen KW-13)
Length = 1100
Score = 35.8 bits (81), Expect = 0.13
Identities = 24/64 (37%), Positives = 28/64 (43%), Gaps = 1/64 (1%)
Frame = +2
Query: 443 APYPYPQKKPL-NDNEAPMSLPPKYEEFDGSSSQESAPPLPPNGPNDLFLPEIPSDQNNT 619
AP P P L + EAP S PP+ GS APPLP + P P P +
Sbjct: 541 APPPPPPLPGLPSPQEAPPSAPPQAPPLPGSPEPPPAPPLPGDLPPPPPPPPPPPGTDGP 600
Query: 620 NPPP 631
PPP
Sbjct: 601 VPPP 604
>sp|Q5R789|ZCHC8_PONPY Zinc finger CCHC domain containing protein 8
Length = 704
Score = 35.0 bits (79), Expect = 0.22
Identities = 33/99 (33%), Positives = 38/99 (38%), Gaps = 19/99 (19%)
Frame = +2
Query: 452 PYPQKKPLNDNEAP--MSLPPKYEEFDGSSSQES----------APPLPPNGPNDLFLPE 595
P QKK N +P M L E GS S ES PPLP P +F P
Sbjct: 426 PKKQKKESNSAGSPADMELDSDMEVPHGSQSSESFQFQPPLPPDTPPLPRGTPPPIFTPP 485
Query: 596 IPSDQNNTNPP------PGSGSVDFDDLN-RRFEELKRR 691
+P P SG+VD D L EE +RR
Sbjct: 486 LPKGTPPLTPSDSPQTRTASGAVDEDALTLEELEEQQRR 524
>sp|Q9JL26|FMNL_MOUSE Formin-like 1 protein (Formin-related protein)
Length = 1094
Score = 35.0 bits (79), Expect = 0.22
Identities = 24/69 (34%), Positives = 29/69 (42%), Gaps = 4/69 (5%)
Frame = +2
Query: 437 SNAPYPYPQKKPL----NDNEAPMSLPPKYEEFDGSSSQESAPPLPPNGPNDLFLPEIPS 604
S P P P PL + EAP S PP G + APPLP + P P + +
Sbjct: 536 SPPPPPPPPPPPLPNLQSQQEAPPSAPPLAPPLPGCAEPPPAPPLPGDLPPPPPPPPLGT 595
Query: 605 DQNNTNPPP 631
D PPP
Sbjct: 596 DGPVPPPPP 604
>sp|Q8IN94|OSA_DROME Trithorax group protein osa (Eyelid protein)
Length = 2716
Score = 34.7 bits (78), Expect = 0.29
Identities = 23/71 (32%), Positives = 30/71 (42%), Gaps = 8/71 (11%)
Frame = +2
Query: 452 PYPQKKPLNDNEAPMSLPPKYEEFDGSSSQE-SAPPLPPNGPNDLFLPEIPSDQNN---- 616
P Q++ AP + PP G+++ S PP P N N+ P I Q N
Sbjct: 8 PQTQQQQQGGAPAPAATPPSAGAAPGAATPPTSGPPTPNNNSNNGSDPSIQQQQQNVAPH 67
Query: 617 ---TNPPPGSG 640
PPPGSG
Sbjct: 68 PYGAPPPPGSG 78
Score = 30.8 bits (68), Expect = 4.2
Identities = 23/71 (32%), Positives = 28/71 (39%), Gaps = 9/71 (12%)
Frame = +2
Query: 446 PYPYPQ-----KKPLNDNEAPMSLPPKYEEFDGSSS----QESAPPLPPNGPNDLFLPEI 598
PY Y Q K P P PP+ GS + Q P PP GP P +
Sbjct: 160 PYRYGQPLPGGKPPQQQQPHPQQQPPQQPGPGGSPNRPPQQRYIPGQPPQGPT----PTL 215
Query: 599 PSDQNNTNPPP 631
S ++NPPP
Sbjct: 216 NSLLQSSNPPP 226
>sp|O43313|Y0431_HUMAN Hypothetical protein KIAA0431
Length = 667
Score = 34.3 bits (77), Expect = 0.38
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = +2
Query: 434 ISNAPYPYPQKKPLNDNEAPMSLPPKYEEFDGSSSQESAPPLPPNGPNDLFLPE 595
++N P P P + L +E + L P +E+ GS++ + PP P L LP+
Sbjct: 91 LNNQPIPRPDTQELEASE--IKLEPSFEDSCGSNTDKQTLTTPPRYPQKLLLPK 142
>sp|Q95107|WASL_BOVIN Neural Wiskott-Aldrich syndrome protein (N-WASP)
Length = 505
Score = 33.9 bits (76), Expect = 0.50
Identities = 24/79 (30%), Positives = 30/79 (37%), Gaps = 1/79 (1%)
Frame = +2
Query: 455 YPQKKPLNDNEAPMSLPPKYEEFDGSSS-QESAPPLPPNGPNDLFLPEIPSDQNNTNPPP 631
YP P + AP PP S S PP PP P P +PSD ++ P P
Sbjct: 343 YPPPLPALPSSAPSGPPPPPPPLSVSGSVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTP 402
Query: 632 GSGSVDFDDLNRRFEELKR 688
D R +LK+
Sbjct: 403 AGSKAALLDQIREGAQLKK 421
Score = 31.6 bits (70), Expect = 2.5
Identities = 23/63 (36%), Positives = 26/63 (41%)
Frame = +2
Query: 443 APYPYPQKKPLNDNEAPMSLPPKYEEFDGSSSQESAPPLPPNGPNDLFLPEIPSDQNNTN 622
AP P P + P AP PP S APP PPN LP +PS +
Sbjct: 309 APPPPPSRAP---TAAPPPPPP-------SRPGVGAPPPPPNRMYPPPLPALPSSAPSGP 358
Query: 623 PPP 631
PPP
Sbjct: 359 PPP 361
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,675,094
Number of Sequences: 369166
Number of extensions: 1995627
Number of successful extensions: 7019
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6852
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7066925600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)