Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_A01
(786 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8K021|SCAM1_MOUSE Secretory carrier-associated membrane... 96 9e-20
sp|P56603|SCAM1_RAT Secretory carrier-associated membrane p... 96 1e-19
sp|O15126|SCAM1_HUMAN Secretory carrier-associated membrane... 96 1e-19
sp|Q9JKD3|SCAM5_MOUSE Secretory carrier-associated membrane... 93 7e-19
sp|Q9JKE3|SCAM5_RAT Secretory carrier-associated membrane p... 93 7e-19
sp|Q8TAC9|SCAM5_HUMAN Secretory carrier-associated membrane... 92 1e-18
sp|O77735|SCAM1_PIG Secretory carrier-associated membrane p... 92 2e-18
sp|Q9ET20|SCAM4_RAT Secretory carrier-associated membrane p... 86 1e-16
sp|Q9ERN0|SCAM2_MOUSE Secretory carrier-associated membrane... 85 2e-16
sp|Q9JKV5|SCAM4_MOUSE Secretory carrier-associated membrane... 85 2e-16
>sp|Q8K021|SCAM1_MOUSE Secretory carrier-associated membrane protein 1 (Secretory carrier
membrane protein 1)
Length = 338
Score = 96.3 bits (238), Expect = 9e-20
Identities = 72/268 (26%), Positives = 105/268 (39%), Gaps = 16/268 (5%)
Frame = +2
Query: 29 NNSDLNPFAATPTRNDPPPYRPTVQTETLNDFNPFENE----PAVIQAHNTES------K 178
NN +P TRN PP L+++NPF + P ++ N + K
Sbjct: 15 NNPFKDPSVTQVTRNVPPG---------LDEYNPFSDSRTPPPGSVKMPNVPNTQPAIMK 65
Query: 179 KNEVNNSFQTITN------AXXXXXXXXXXXXANELSXXXXXXXXXXXXXXGSIKNFPPL 340
E + ++ IT A A EL G N+PPL
Sbjct: 66 PTEEHPAYTQITKEHALAQAELLKRQEELERKAAELDRREREMQNLSQH--GRKNNWPPL 123
Query: 341 PSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYMITQDVXXXX 520
PS FP+ PCFYQ+ V+IP E+++ V+ +Y+WM ++V + +NI G L++ V
Sbjct: 124 PSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYLWMFHAVTLFLNIFGCLAWFC---VDSSR 180
Query: 521 XXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXX 700
P S++CW+R LY A
Sbjct: 181 AVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAVHVLQAAGFH 240
Query: 701 XXXSCGWINSLAELKTNVGVGAFMCIIA 784
+CGWI+SL L N+ VG M IIA
Sbjct: 241 NWGNCGWISSLTGLNKNIPVGIMMIIIA 268
>sp|P56603|SCAM1_RAT Secretory carrier-associated membrane protein 1 (Secretory carrier
membrane protein 1) (SCAMP 37)
Length = 338
Score = 95.9 bits (237), Expect = 1e-19
Identities = 72/268 (26%), Positives = 102/268 (38%), Gaps = 16/268 (5%)
Frame = +2
Query: 29 NNSDLNPFAATPTRNDPPPYRPTVQTETLNDFNPFENE----------PAVIQAHNTESK 178
NN +P TRN PP L+++NPF + P V K
Sbjct: 15 NNPFKDPSVTQVTRNVPPG---------LDEYNPFSDSRTPPPGGVKMPNVPNTQPAIMK 65
Query: 179 KNEVNNSFQTITN------AXXXXXXXXXXXXANELSXXXXXXXXXXXXXXGSIKNFPPL 340
E + ++ IT A A EL G N+PPL
Sbjct: 66 PTEEHPAYTQITKEHALAQAELLKRQEELERKAAELDRREREMQNLSQH--GRKNNWPPL 123
Query: 341 PSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYMITQDVXXXX 520
PS FP+ PCFYQ+ V+IP E+++ V+ +Y+WM ++V + +NI G L++ V
Sbjct: 124 PSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYLWMFHAVTLFLNIFGCLAWFC---VDSSR 180
Query: 521 XXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXX 700
P S++CW+R LY A
Sbjct: 181 AVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAVHVLQAAGFH 240
Query: 701 XXXSCGWINSLAELKTNVGVGAFMCIIA 784
+CGWI+SL L N+ VG M IIA
Sbjct: 241 NWGNCGWISSLTGLNKNIPVGIMMIIIA 268
>sp|O15126|SCAM1_HUMAN Secretory carrier-associated membrane protein 1 (Secretory carrier
membrane protein 1)
Length = 338
Score = 95.5 bits (236), Expect = 1e-19
Identities = 50/157 (31%), Positives = 72/157 (45%)
Frame = +2
Query: 314 GSIKNFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYM 493
G N+PPLPS FP+ PCFYQ+ V+IP E+++ V+ +Y+WM ++V + +NI G L++
Sbjct: 115 GRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVKLMYYLWMFHAVTLFLNIFGCLAWF 174
Query: 494 ITQDVXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXX 673
V P S++CW+R LY A
Sbjct: 175 C---VDSARAVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAV 231
Query: 674 XXXXXXXXXXXXSCGWINSLAELKTNVGVGAFMCIIA 784
+CGWI+SL L N+ VG M IIA
Sbjct: 232 HVLQAAGFHNWGNCGWISSLTGLNQNIPVGIMMIIIA 268
>sp|Q9JKD3|SCAM5_MOUSE Secretory carrier-associated membrane protein 5 (Secretory carrier
membrane protein 5)
Length = 235
Score = 93.2 bits (230), Expect = 7e-19
Identities = 45/153 (29%), Positives = 69/153 (45%)
Frame = +2
Query: 320 IKNFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYMIT 499
+ NFPPLP + P+KPCFYQ+ + +IP ++ + + +Y+WM SV + VN++G L+++I
Sbjct: 5 VNNFPPLPKFIPLKPCFYQDFEADIPPQHLSLTKRLYYLWMLNSVTLAVNLVGCLAWLI- 63
Query: 500 QDVXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXXXX 679
P SY+CWFR +YKA A
Sbjct: 64 ---GGGGATNFGLAFLWLILFTPCSYVCWFRPIYKAFKTDSSFSFMAFFFTFMAQLVISI 120
Query: 680 XXXXXXXXXXSCGWINSLAELKTNVGVGAFMCI 778
CGWI +++ TN+G M I
Sbjct: 121 IQAVGIPGWGVCGWIATISFFGTNIGSAVVMLI 153
>sp|Q9JKE3|SCAM5_RAT Secretory carrier-associated membrane protein 5 (Secretory carrier
membrane protein 5)
Length = 235
Score = 93.2 bits (230), Expect = 7e-19
Identities = 45/153 (29%), Positives = 69/153 (45%)
Frame = +2
Query: 320 IKNFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYMIT 499
+ NFPPLP + P+KPCFYQ+ + +IP ++ + + +Y+WM SV + VN++G L+++I
Sbjct: 5 VNNFPPLPKFIPLKPCFYQDFEADIPPQHLSLTKRLYYLWMLNSVTLAVNLVGCLAWLI- 63
Query: 500 QDVXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXXXX 679
P SY+CWFR +YKA A
Sbjct: 64 ---GGGGATNFGLAFLWLILFTPCSYVCWFRPIYKAFKTDSSFSFMAFFFTFMAQLVISI 120
Query: 680 XXXXXXXXXXSCGWINSLAELKTNVGVGAFMCI 778
CGWI +++ TN+G M I
Sbjct: 121 IQAVGIPGWGVCGWIATISFFGTNIGSAVVMLI 153
>sp|Q8TAC9|SCAM5_HUMAN Secretory carrier-associated membrane protein 5 (Secretory carrier
membrane protein 5)
Length = 235
Score = 92.4 bits (228), Expect = 1e-18
Identities = 45/153 (29%), Positives = 69/153 (45%)
Frame = +2
Query: 320 IKNFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYMIT 499
+ NFPPLP + P+KPCFYQ+ + +IP ++ + + +Y+WM SV + VN++G L+++I
Sbjct: 5 VNNFPPLPKFIPLKPCFYQDFEADIPPQHVSMTKRLYYLWMLNSVTLAVNLVGCLAWLI- 63
Query: 500 QDVXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXXXX 679
P SY+CWFR +YKA A
Sbjct: 64 ---GGGGATNFGLAFLWLILFTPCSYVCWFRPIYKAFKTDSSFSFMAFFFTFMAQLVISI 120
Query: 680 XXXXXXXXXXSCGWINSLAELKTNVGVGAFMCI 778
CGWI +++ TN+G M I
Sbjct: 121 IQAVGIPGWGVCGWIATISFFGTNIGSAVVMLI 153
>sp|O77735|SCAM1_PIG Secretory carrier-associated membrane protein 1 (Secretory carrier
membrane protein 1)
Length = 338
Score = 92.0 bits (227), Expect = 2e-18
Identities = 48/157 (30%), Positives = 71/157 (45%)
Frame = +2
Query: 314 GSIKNFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYM 493
G N+PPLP FP+ PCFYQ+ V+IP E+++ V+ +Y+WM ++V + +NI G L++
Sbjct: 115 GRKNNWPPLPGNFPVGPCFYQDFSVDIPVEFQKTVKIMYYLWMFHAVTLFLNIFGCLAWF 174
Query: 494 ITQDVXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXX 673
V P S++CW+R LY A
Sbjct: 175 C---VDPSRGVDFGLSILWFLLFTPCSFVCWYRPLYGAFRSDSSFRFFVFFFVYICQFAV 231
Query: 674 XXXXXXXXXXXXSCGWINSLAELKTNVGVGAFMCIIA 784
+CGWI+SL L ++ VG M IIA
Sbjct: 232 HVLQAAGFHNWGNCGWISSLTGLNQSIPVGIMMIIIA 268
>sp|Q9ET20|SCAM4_RAT Secretory carrier-associated membrane protein 4 (Secretory carrier
membrane protein 4) (SCAMP-4)
Length = 230
Score = 85.9 bits (211), Expect = 1e-16
Identities = 46/157 (29%), Positives = 66/157 (42%)
Frame = +2
Query: 314 GSIKNFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYM 493
G NFPPLP + P+KPCFYQ+ EIP E++ +V+ + +WM Y + VN++ L++
Sbjct: 3 GKENNFPPLPHFLPLKPCFYQDFSDEIPVEHQVLVKRIYRLWMFYCTTLGVNLVACLAWW 62
Query: 494 ITQDVXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXX 673
I P SY+CWFR YKA A
Sbjct: 63 IA----GGAGANFGLAMLWLVLFTPCSYVCWFRPAYKAFRADSSFNFMAFFFIFGAQFVL 118
Query: 674 XXXXXXXXXXXXSCGWINSLAELKTNVGVGAFMCIIA 784
+CGW+ ++ T+VG M I A
Sbjct: 119 TVIQAIGFSGWGACGWLATIGFFGTSVGAAVVMLIPA 155
>sp|Q9ERN0|SCAM2_MOUSE Secretory carrier-associated membrane protein 2 (Secretory carrier
membrane protein 2)
Length = 329
Score = 85.1 bits (209), Expect = 2e-16
Identities = 36/94 (38%), Positives = 57/94 (60%)
Frame = +2
Query: 326 NFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYMITQD 505
N+PPLPSW P+KPCFYQ+ EIPA+Y++I + +Y+WM +SV + +N++ L++ T D
Sbjct: 119 NWPPLPSWCPVKPCFYQDFSTEIPADYQRICKMLYYLWMLHSVTLFLNLLACLAW-FTSD 177
Query: 506 VXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKA 607
P +++CW+R +YKA
Sbjct: 178 A--ANGTAFGLSILWFLIFTPCAFLCWYRPIYKA 209
>sp|Q9JKV5|SCAM4_MOUSE Secretory carrier-associated membrane protein 4 (Secretory carrier
membrane protein 4) (SC4)
Length = 230
Score = 85.1 bits (209), Expect = 2e-16
Identities = 45/157 (28%), Positives = 66/157 (42%)
Frame = +2
Query: 314 GSIKNFPPLPSWFPMKPCFYQEIDVEIPAEYRQIVRTGFYIWMAYSVVMLVNIIGTLSYM 493
G NFPPLP + P+KPCFYQ+ EIP E++ +V+ + +WM Y + VN++ L++
Sbjct: 3 GKENNFPPLPPFLPLKPCFYQDFSDEIPVEHQVLVKRIYRLWMFYCATLGVNLVACLAWW 62
Query: 494 ITQDVXXXXXXXXXXXXXXXXXXVPASYICWFRSLYKAXXXXXXXXXXXXXXXXXAXXXX 673
I P SY+CWFR YKA A
Sbjct: 63 IA----GGAGANFGLALLWLVLFTPCSYVCWFRPAYKAFRADSSFNFMTFFFIFGAQFVL 118
Query: 674 XXXXXXXXXXXXSCGWINSLAELKTNVGVGAFMCIIA 784
+CGW+ ++ T+VG M + A
Sbjct: 119 TVIQAIGFSGWGACGWLAAVGFFGTSVGAAVVMLVPA 155
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,573,363
Number of Sequences: 369166
Number of extensions: 1316726
Number of successful extensions: 3527
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3426
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3505
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7405750800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)