Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_024_P13
(626 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99JY0|ECHB_MOUSE Trifunctional enzyme beta subunit, mit... 284 1e-76
sp|Q60587|ECHB_RAT Trifunctional enzyme beta subunit, mitoc... 283 3e-76
sp|O46629|ECHB_BOVIN Trifunctional enzyme beta subunit, mit... 283 3e-76
sp|Q5R1W7|ECHB_PANTR Trifunctional enzyme beta subunit, mit... 283 3e-76
sp|Q8HXX4|ECHB_MACFA Trifunctional enzyme beta subunit, mit... 283 3e-76
sp|P34255|YKA3_CAEEL Hypothetical protein B0303.3 in chromo... 279 4e-75
sp|P76503|FADI_ECOLI 3-ketoacyl-CoA thiolase (Fatty acid ox... 168 1e-41
sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondr... 119 6e-27
sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochon... 119 8e-27
sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Perox... 114 2e-25
>sp|Q99JY0|ECHB_MOUSE Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 284 bits (727), Expect = 1e-76
Identities = 136/187 (72%), Positives = 160/187 (85%)
Frame = +3
Query: 12 EKMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQD 191
E+MA LKPAFIKP+GT+TAAN+SFLTDGASA L+ +E++A MG+KPKAYLRDFIYVSQD
Sbjct: 288 EQMAKLKPAFIKPYGTVTAANSSFLTDGASAMLIMSEDRALAMGYKPKAYLRDFIYVSQD 347
Query: 192 PKDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRP 371
PKDQLLLGP YATPK+L++ LT+ DI AFEFHEAF+GQILAN KAMD D+F + YM R
Sbjct: 348 PKDQLLLGPTYATPKVLEKAGLTMNDIDAFEFHEAFSGQILANFKAMDSDWFAQNYMGRK 407
Query: 372 AKYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHA 551
K GS PL++ N WGGSLS+GHPFGATG RLV AANRL K+ GQ+ALVAACAAGGQGHA
Sbjct: 408 TKVGSPPLEKFNIWGGSLSLGHPFGATGCRLVMAAANRLRKDGGQYALVAACAAGGQGHA 467
Query: 552 ILIENYP 572
+++E YP
Sbjct: 468 MIVEAYP 474
>sp|Q60587|ECHB_RAT Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 283 bits (724), Expect = 3e-76
Identities = 135/187 (72%), Positives = 160/187 (85%)
Frame = +3
Query: 12 EKMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQD 191
E+MA LKPAFIKP+GT+TAAN+SFLTDGASA L+ +E++A MG+KPKAYLRDFIYVSQD
Sbjct: 288 EQMAKLKPAFIKPYGTVTAANSSFLTDGASAMLIMSEDRALAMGYKPKAYLRDFIYVSQD 347
Query: 192 PKDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRP 371
PKDQLLLGP YATPK+L++ LT+ DI AFEFHEAF+GQILAN KAMD D+F + YM R
Sbjct: 348 PKDQLLLGPTYATPKVLEKAGLTMNDIDAFEFHEAFSGQILANFKAMDSDWFAQNYMGRK 407
Query: 372 AKYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHA 551
K G+ PL++ N WGGSLS+GHPFGATG RLV AANRL K+ GQ+ALVAACAAGGQGHA
Sbjct: 408 TKVGAPPLEKFNIWGGSLSLGHPFGATGCRLVMAAANRLRKDGGQYALVAACAAGGQGHA 467
Query: 552 ILIENYP 572
+++E YP
Sbjct: 468 MIVEAYP 474
>sp|O46629|ECHB_BOVIN Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 283 bits (724), Expect = 3e-76
Identities = 136/187 (72%), Positives = 158/187 (84%)
Frame = +3
Query: 12 EKMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQD 191
++MA LKPAFIKP+GT+TAAN+SFLTDGASA L+ EEKA MG+KPKAYLRDF+YVSQD
Sbjct: 288 DQMAKLKPAFIKPYGTVTAANSSFLTDGASAVLIMAEEKALAMGYKPKAYLRDFMYVSQD 347
Query: 192 PKDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRP 371
PKDQLLLGP YATPK+L++ LT+ DI FEFHEAF+GQILANLKAMD D+F + YM R
Sbjct: 348 PKDQLLLGPTYATPKVLEKAGLTMNDIDVFEFHEAFSGQILANLKAMDSDWFAQNYMGRK 407
Query: 372 AKYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHA 551
AK G PL++ N WGGSLS+GHPFGATG RLV AANRL KE GQ+ LVAACAAGGQGHA
Sbjct: 408 AKVGLPPLEKFNNWGGSLSLGHPFGATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHA 467
Query: 552 ILIENYP 572
+++E YP
Sbjct: 468 MIVEAYP 474
>sp|Q5R1W7|ECHB_PANTR Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
sp|P55084|ECHB_HUMAN Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 283 bits (723), Expect = 3e-76
Identities = 136/187 (72%), Positives = 157/187 (83%)
Frame = +3
Query: 12 EKMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQD 191
E+MA LKPAFIKP+GT+TAAN+SFLTDGASA L+ EEKA MG+KPKAYLRDF+YVSQD
Sbjct: 288 EQMAKLKPAFIKPYGTVTAANSSFLTDGASAMLIMAEEKALAMGYKPKAYLRDFMYVSQD 347
Query: 192 PKDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRP 371
PKDQLLLGP YATPK+L++ LT+ DI AFEFHEAF+GQILAN KAMD D+F + YM R
Sbjct: 348 PKDQLLLGPTYATPKVLEKAGLTMNDIDAFEFHEAFSGQILANFKAMDSDWFAENYMGRK 407
Query: 372 AKYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHA 551
K G PL++ N WGGSLS+GHPFGATG RLV AANRL KE GQ+ LVAACAAGGQGHA
Sbjct: 408 TKVGLPPLEKFNNWGGSLSLGHPFGATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHA 467
Query: 552 ILIENYP 572
+++E YP
Sbjct: 468 MIVEAYP 474
>sp|Q8HXX4|ECHB_MACFA Trifunctional enzyme beta subunit, mitochondrial precursor
(TP-beta) [Includes: 3-ketoacyl-CoA thiolase (Acetyl-CoA
acyltransferase) (Beta-ketothiolase)]
Length = 475
Score = 283 bits (723), Expect = 3e-76
Identities = 136/187 (72%), Positives = 157/187 (83%)
Frame = +3
Query: 12 EKMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQD 191
E+MA LKPAFIKP+GT+TAAN+SFLTDGASA L+ EEKA MG+KPKAYLRDF+YVSQD
Sbjct: 288 EQMAKLKPAFIKPYGTVTAANSSFLTDGASAMLIMAEEKALAMGYKPKAYLRDFMYVSQD 347
Query: 192 PKDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRP 371
PKDQLLLGP YATPK+L++ LT+ DI AFEFHEAF+GQILAN KAMD D+F + YM R
Sbjct: 348 PKDQLLLGPTYATPKVLEKAGLTMNDIDAFEFHEAFSGQILANFKAMDSDWFAENYMGRK 407
Query: 372 AKYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHA 551
K G PL++ N WGGSLS+GHPFGATG RLV AANRL KE GQ+ LVAACAAGGQGHA
Sbjct: 408 TKVGLPPLEKFNNWGGSLSLGHPFGATGCRLVMAAANRLRKEGGQYGLVAACAAGGQGHA 467
Query: 552 ILIENYP 572
+++E YP
Sbjct: 468 MIVEAYP 474
>sp|P34255|YKA3_CAEEL Hypothetical protein B0303.3 in chromosome III
Length = 448
Score = 279 bits (714), Expect = 4e-75
Identities = 131/186 (70%), Positives = 158/186 (84%)
Frame = +3
Query: 12 EKMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQD 191
EK+++LKPAF+KPHGT+TAANAS+LTDGASA L+ TEE A G+KPKAYLRD++YV+QD
Sbjct: 261 EKLSSLKPAFVKPHGTVTAANASYLTDGASAALIMTEEYALANGYKPKAYLRDYLYVAQD 320
Query: 192 PKDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRP 371
PKDQLLL PAY PK+LD+ LTLKD+ FE HEAFAGQ+LANL AMD D+FCKE MKR
Sbjct: 321 PKDQLLLSPAYVIPKLLDKAGLTLKDVDVFEIHEAFAGQVLANLNAMDSDYFCKEQMKRS 380
Query: 372 AKYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHA 551
K+G +P+D+LN WGGSLSIGHPFGATGVRL T +A+RL +EKGQ+A++AACAAGG G
Sbjct: 381 GKFGRVPMDKLNLWGGSLSIGHPFGATGVRLATHSAHRLKEEKGQYAVIAACAAGGHGVG 440
Query: 552 ILIENY 569
+LIE Y
Sbjct: 441 MLIEAY 446
>sp|P76503|FADI_ECOLI 3-ketoacyl-CoA thiolase (Fatty acid oxidation complex beta subunit)
(Beta-ketothiolase) (Acetyl-CoA acyltransferase)
(Acyl-CoA ligase) (ACSs)
Length = 436
Score = 168 bits (425), Expect = 1e-41
Identities = 82/181 (45%), Positives = 119/181 (65%)
Frame = +3
Query: 21 ATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDPKD 200
A L+PAF + HGT+TAAN++ LTDGA+A +L TE +AK++G P YLR + + + D
Sbjct: 254 AKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQ 313
Query: 201 QLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPAKY 380
+LLGPA++TP L+R LT+ D+T + HEAFA Q LAN++ + + F +E + R
Sbjct: 314 DMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHAT 373
Query: 381 GSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAILI 560
G + + N GGS++ GHPF ATG R++T + L + G F LV ACAAGG G A+++
Sbjct: 374 GEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVL 433
Query: 561 E 563
E
Sbjct: 434 E 434
>sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 119 bits (298), Expect = 6e-27
Identities = 74/183 (40%), Positives = 105/183 (57%)
Frame = +3
Query: 15 KMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDP 194
K+ LK F K +GT+TAANAS L DGA+A +L T E A+++ KP A + F + DP
Sbjct: 260 KVPKLKTVFQKENGTVTAANASTLNDGAAAVVLMTAEAAQRLKVKPLARIAAFADAAVDP 319
Query: 195 KDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPA 374
D L PAYA PK+L L +DI +E +EAF+ +LAN+K ++ D P
Sbjct: 320 ID-FPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLEID---------PQ 369
Query: 375 KYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAI 554
K +N GG++S+GHP G +G R+V A+ L ++G+F L + C GG A+
Sbjct: 370 K--------VNVHGGAVSLGHPIGMSGARIVVHLAHAL--KQGEFGLASICNGGGGASAV 419
Query: 555 LIE 563
LIE
Sbjct: 420 LIE 422
>sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 119 bits (297), Expect = 8e-27
Identities = 75/183 (40%), Positives = 104/183 (56%)
Frame = +3
Query: 15 KMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQDP 194
K+ LK F K +GTITAANAS L DGA+A +L T E A+++ KP A + F + DP
Sbjct: 260 KVPKLKTVFQKENGTITAANASTLNDGAAALVLMTAEAAQRLNVKPLARIAAFADAAVDP 319
Query: 195 KDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRPA 374
D L PAYA PK+L L +DI +E +EAF+ +LAN+K ++ D P
Sbjct: 320 ID-FPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKMLEID---------PQ 369
Query: 375 KYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHAI 554
K +N GG++S+GHP G +G R+V A+ L + G+F L + C GG A+
Sbjct: 370 K--------VNIHGGAVSLGHPIGMSGARIVVHMAHAL--KPGEFGLASICNGGGGASAL 419
Query: 555 LIE 563
LIE
Sbjct: 420 LIE 422
>sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IA)
Length = 403
Score = 114 bits (286), Expect = 2e-25
Identities = 64/184 (34%), Positives = 103/184 (55%)
Frame = +3
Query: 12 EKMATLKPAFIKPHGTITAANASFLTDGASACLLTTEEKAKQMGWKPKAYLRDFIYVSQD 191
E++ + + F K +GT+TA NAS L DG +A +L +E K KQ+G KP A + + ++
Sbjct: 230 ERLKSARTVFQKENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKISGWGEAART 289
Query: 192 PKDQLLLGPAYATPKILDRNKLTLKDITAFEFHEAFAGQILANLKAMDCDFFCKEYMKRP 371
P D + PA A PK + LT+ + FE +EAF+ LAN
Sbjct: 290 PFD-FTIAPALAVPKAVKHAGLTVDRVDFFELNEAFSVVGLAN----------------- 331
Query: 372 AKYGSLPLDRLNTWGGSLSIGHPFGATGVRLVTTAANRLIKEKGQFALVAACAAGGQGHA 551
A+ ++PL++LN +GG++++GHP G +G R++ T + L +E G+F + C GG A
Sbjct: 332 AELVNIPLEKLNVYGGAVAMGHPLGCSGARIIVTLLSVLTQEGGRFGVAGVCNGGGGASA 391
Query: 552 ILIE 563
++IE
Sbjct: 392 VVIE 395
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,461,020
Number of Sequences: 369166
Number of extensions: 1279092
Number of successful extensions: 3483
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3360
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)